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The map label for this gene is dfrA [H]

Identifier: 226224078

GI number: 226224078

Start: 1493922

End: 1494950

Strand: Reverse

Name: dfrA [H]

Synonym: Lm4b_01487

Alternate gene names: 226224078

Gene position: 1494950-1493922 (Counterclockwise)

Preceding gene: 226224080

Following gene: 226224077

Centisome position: 51.33

GC content: 36.54

Gene sequence:

>1029_bases
ATGAAAAATAACGTATTAGTAACAGGCGGAACTGGATTTTTAGGAATGCATATTATCTTTCAATTATTGCAACAAGGCTA
TCAAGTCAAAACAACCGTACGCTCTTTAAAAAGCAAAGAAAAAGTAATAGAAGTACTGCAAAATAATGGAATAACGGATT
TTACGCAATTATCCTTTGTCGAACTTGATTTATCCAAAGATGAAGGATGGAAAGAAGCAATGCTCGATTGTAAATATGTG
CTAAGTGTCGCATCTCCAGTATTTTTCGGTAAATTTAAAAATGAAGAAGAATTAATACGCCCAGCAATAGAAGGTATTAC
AAGAATTTTACAGGCCGCAAAAGAGGCAAAAGTAAAACGAGTAGTTATGACTTCGAATTTTGGCGCTATTGGTTTTAGCA
ACGCAGATAAAAATAGCATTACAACAGAAGCATATTGGACCGATGAACTCGCTAAGGGTTTGTCAGCATATGAAAAATCT
AAACTAATTGCAGAAAAAGAAGCTTGGAAATTCATGGAAAATGAAACAGAATTGGAATTTGCTACCATCAATCCGGTTGC
CATTTTTGGTCCATCACAAAGCAGTCATGTCTCCGGAAGCTTTGACTTATTAAAAAATCTGTTAAATGGTTCTATGAAAC
GTATCATAAATATTCCGTTAAACGTAGTAGATGCACGAGATGTTGCCGATTTACATATTCGCGCAATGATTACTCCTGAA
GCAAACGGAGAACGTTTTATTGCTTCAGCTGACGGAGAAATTAGTATGGCAGATATCGCACATTTGCTTCAACGAGAGCG
TCCTGAACTAGTGAGTAAAATGCCGAAAAAAACATTGCCAAATGCCGCTATAAGAGCAGCTGCTATATTTAGTAAACACG
CCAAAGAAGGCGAGTTGATGATTAATATGAATCGGCAAATAAGCAATTCAAAAGCGAGAGATCTTTTAGGATGGCAGCCG
ATTTCGACAAAAGAAGAAGCCGTTCTTGCTGCAGTGGATAGTTTGGCAAAGTATGGTTTATTAGATTAA

Upstream 100 bases:

>100_bases
TAAATAATTTACTTGCCCTACACTATACTCTAGGGTTTAAAGTAAGTATAAAGTTAATAAAGGAAATAAGCAAAATTAAC
TACTATTGGAGGAATTCACG

Downstream 100 bases:

>100_bases
AAGCGAATCCTGCACCAAAGCACTGGAAAATATGTTAAACTGACTAATGGACAACGAAGCAAATTTTAAGTGCAAGGAAT
TGATAAAAATGATAAATACG

Product: oxidoreductase

Products: NA

Alternate protein names: DFR; Dihydrokaempferol 4-reductase [H]

Number of amino acids: Translated: 342; Mature: 342

Protein sequence:

>342_residues
MKNNVLVTGGTGFLGMHIIFQLLQQGYQVKTTVRSLKSKEKVIEVLQNNGITDFTQLSFVELDLSKDEGWKEAMLDCKYV
LSVASPVFFGKFKNEEELIRPAIEGITRILQAAKEAKVKRVVMTSNFGAIGFSNADKNSITTEAYWTDELAKGLSAYEKS
KLIAEKEAWKFMENETELEFATINPVAIFGPSQSSHVSGSFDLLKNLLNGSMKRIINIPLNVVDARDVADLHIRAMITPE
ANGERFIASADGEISMADIAHLLQRERPELVSKMPKKTLPNAAIRAAAIFSKHAKEGELMINMNRQISNSKARDLLGWQP
ISTKEEAVLAAVDSLAKYGLLD

Sequences:

>Translated_342_residues
MKNNVLVTGGTGFLGMHIIFQLLQQGYQVKTTVRSLKSKEKVIEVLQNNGITDFTQLSFVELDLSKDEGWKEAMLDCKYV
LSVASPVFFGKFKNEEELIRPAIEGITRILQAAKEAKVKRVVMTSNFGAIGFSNADKNSITTEAYWTDELAKGLSAYEKS
KLIAEKEAWKFMENETELEFATINPVAIFGPSQSSHVSGSFDLLKNLLNGSMKRIINIPLNVVDARDVADLHIRAMITPE
ANGERFIASADGEISMADIAHLLQRERPELVSKMPKKTLPNAAIRAAAIFSKHAKEGELMINMNRQISNSKARDLLGWQP
ISTKEEAVLAAVDSLAKYGLLD
>Mature_342_residues
MKNNVLVTGGTGFLGMHIIFQLLQQGYQVKTTVRSLKSKEKVIEVLQNNGITDFTQLSFVELDLSKDEGWKEAMLDCKYV
LSVASPVFFGKFKNEEELIRPAIEGITRILQAAKEAKVKRVVMTSNFGAIGFSNADKNSITTEAYWTDELAKGLSAYEKS
KLIAEKEAWKFMENETELEFATINPVAIFGPSQSSHVSGSFDLLKNLLNGSMKRIINIPLNVVDARDVADLHIRAMITPE
ANGERFIASADGEISMADIAHLLQRERPELVSKMPKKTLPNAAIRAAAIFSKHAKEGELMINMNRQISNSKARDLLGWQP
ISTKEEAVLAAVDSLAKYGLLD

Specific function: Galactose metabolism; third step. [C]

COG id: COG0451

COG function: function code MG; Nucleoside-diphosphate-sugar epimerases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dihydroflavonol-4-reductase family [H]

Homologues:

Organism=Homo sapiens, GI193211614, Length=232, Percent_Identity=28.0172413793103, Blast_Score=68, Evalue=1e-11,
Organism=Homo sapiens, GI8393516, Length=232, Percent_Identity=28.0172413793103, Blast_Score=68, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI71987463, Length=351, Percent_Identity=32.7635327635328, Blast_Score=176, Evalue=1e-44,
Organism=Saccharomyces cerevisiae, GI6320750, Length=360, Percent_Identity=30, Blast_Score=134, Evalue=2e-32,
Organism=Saccharomyces cerevisiae, GI6324421, Length=297, Percent_Identity=31.986531986532, Blast_Score=123, Evalue=4e-29,
Organism=Saccharomyces cerevisiae, GI6321281, Length=352, Percent_Identity=29.5454545454545, Blast_Score=122, Evalue=1e-28,
Organism=Saccharomyces cerevisiae, GI6321399, Length=338, Percent_Identity=28.4023668639053, Blast_Score=117, Evalue=4e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001509
- InterPro:   IPR017829
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: =1.1.1.219 [H]

Molecular weight: Translated: 37880; Mature: 37880

Theoretical pI: Translated: 7.09; Mature: 7.09

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNNVLVTGGTGFLGMHIIFQLLQQGYQVKTTVRSLKSKEKVIEVLQNNGITDFTQLSFV
CCCCEEEECCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHEEEEE
ELDLSKDEGWKEAMLDCKYVLSVASPVFFGKFKNEEELIRPAIEGITRILQAAKEAKVKR
EEECCCCCCHHHHHHHHHHHHHHHCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHE
VVMTSNFGAIGFSNADKNSITTEAYWTDELAKGLSAYEKSKLIAEKEAWKFMENETELEF
EEEECCCCCEECCCCCCCCCEEHHEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEE
ATINPVAIFGPSQSSHVSGSFDLLKNLLNGSMKRIINIPLNVVDARDVADLHIRAMITPE
EEECEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCEEEEEEECCC
ANGERFIASADGEISMADIAHLLQRERPELVSKMPKKTLPNAAIRAAAIFSKHAKEGELM
CCCCEEEEECCCCEEHHHHHHHHHHCCHHHHHHCCHHHCCHHHHHHHHHHHHCCCCCCEE
INMNRQISNSKARDLLGWQPISTKEEAVLAAVDSLAKYGLLD
EEECCCCCCCHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MKNNVLVTGGTGFLGMHIIFQLLQQGYQVKTTVRSLKSKEKVIEVLQNNGITDFTQLSFV
CCCCEEEECCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHEEEEE
ELDLSKDEGWKEAMLDCKYVLSVASPVFFGKFKNEEELIRPAIEGITRILQAAKEAKVKR
EEECCCCCCHHHHHHHHHHHHHHHCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHE
VVMTSNFGAIGFSNADKNSITTEAYWTDELAKGLSAYEKSKLIAEKEAWKFMENETELEF
EEEECCCCCEECCCCCCCCCEEHHEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEE
ATINPVAIFGPSQSSHVSGSFDLLKNLLNGSMKRIINIPLNVVDARDVADLHIRAMITPE
EEECEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCEEEEEEECCC
ANGERFIASADGEISMADIAHLLQRERPELVSKMPKKTLPNAAIRAAAIFSKHAKEGELM
CCCCEEEEECCCCEEHHHHHHHHHHCCHHHHHHCCHHHCCHHHHHHHHHHHHCCCCCCEE
INMNRQISNSKARDLLGWQPISTKEEAVLAAVDSLAKYGLLD
EEECCCCCCCHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]