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The map label for this gene is pflC

Identifier: 226224008

GI number: 226224008

Start: 1421653

End: 1422399

Strand: Direct

Name: pflC

Synonym: Lm4b_01416

Alternate gene names: 226224008

Gene position: 1421653-1422399 (Clockwise)

Preceding gene: 226224007

Following gene: 226224010

Centisome position: 48.81

GC content: 39.09

Gene sequence:

>747_bases
ATGACAGAGGTTTTAGGAAGAGTTCATTCAGTAGAAACAATGGGAACAGTAGACGGTCCAGGTATCCGCTTTATTGTTTT
TATGCAGGGGTGTTTACTTCGTTGCCAATTTTGTCATAATCCCGATACTTGGAAAATTGGTACTGGCACAGAACGTTCTG
CCCAAGATGTATTTGACGAAGCAATTAAGTATAAAGAGTTTTGGGATGCATCAGGTGGCGGCGTGACAGTTAGTGGCGGT
GAACCGTTGCTTCAAGTAGATTTCCTAATCGAGTTTTTCACGCTATGTAAAGCAGCAGGAGTGCACACAACTATTGATTC
TTGTGGTGGTTGTTTCACACGTGATCCGGAGTTTATTGAGAAATTGGATCGTTTGATGGAAGTAACGGATTTAATTTTAC
TGGATATTAAGCAAATCAACCCAGAAAAGCATTTAAAACTGACAACGAAATCTAATGCACCAATAATCGATTTTGCTCAT
TATTTACGTGACAAAGAGCAACCAATTTGGATTAGACATGTACTAATTCCAACCAAGACAGATGATCCAGAAGATTTAAC
AAAGCTCCACGAATTCATTCAAACTCTTCCAAACGTAAAACAAGTGGATGTGCTTCCATACCATACAATGGGTGTTTACA
AATGGAAAGAAATGGGCATTCGTTATCCGTTAGAAGGAATCGAGGCGCCAGAAGAAGAAGTGGTAGCACTTGCCAATAAA
ATCTTAGAAACTAGTAGTTATAAATAG

Upstream 100 bases:

>100_bases
CGTACAATGCACGAATCCATGTAATAATTAGGCGAGAACGCTTTGGCAAGGATTGCTCACCGTAGCAATCCTTGTTATTT
AGGAAAGGAAGAGGAGAATT

Downstream 100 bases:

>100_bases
TAAAAACCGGCGAACTTAGTATTCGCCGGTTTTTTTATAGTATTTTATACATTTCCTCACACCATTTTAATTCGGTATGA
ATCAAATCTAATCGTCTTGC

Product: pyruvate-formate lyase activating enzyme

Products: NA

Alternate protein names: PFL-activating enzyme

Number of amino acids: Translated: 248; Mature: 247

Protein sequence:

>248_residues
MTEVLGRVHSVETMGTVDGPGIRFIVFMQGCLLRCQFCHNPDTWKIGTGTERSAQDVFDEAIKYKEFWDASGGGVTVSGG
EPLLQVDFLIEFFTLCKAAGVHTTIDSCGGCFTRDPEFIEKLDRLMEVTDLILLDIKQINPEKHLKLTTKSNAPIIDFAH
YLRDKEQPIWIRHVLIPTKTDDPEDLTKLHEFIQTLPNVKQVDVLPYHTMGVYKWKEMGIRYPLEGIEAPEEEVVALANK
ILETSSYK

Sequences:

>Translated_248_residues
MTEVLGRVHSVETMGTVDGPGIRFIVFMQGCLLRCQFCHNPDTWKIGTGTERSAQDVFDEAIKYKEFWDASGGGVTVSGG
EPLLQVDFLIEFFTLCKAAGVHTTIDSCGGCFTRDPEFIEKLDRLMEVTDLILLDIKQINPEKHLKLTTKSNAPIIDFAH
YLRDKEQPIWIRHVLIPTKTDDPEDLTKLHEFIQTLPNVKQVDVLPYHTMGVYKWKEMGIRYPLEGIEAPEEEVVALANK
ILETSSYK
>Mature_247_residues
TEVLGRVHSVETMGTVDGPGIRFIVFMQGCLLRCQFCHNPDTWKIGTGTERSAQDVFDEAIKYKEFWDASGGGVTVSGGE
PLLQVDFLIEFFTLCKAAGVHTTIDSCGGCFTRDPEFIEKLDRLMEVTDLILLDIKQINPEKHLKLTTKSNAPIIDFAHY
LRDKEQPIWIRHVLIPTKTDDPEDLTKLHEFIQTLPNVKQVDVLPYHTMGVYKWKEMGIRYPLEGIEAPEEEVVALANKI
LETSSYK

Specific function: Activation of pyruvate formate-lyase under anaerobic conditions by generation of an organic free radical, using S- adenosylmethionine and reduced flavodoxin as cosubstrates to produce 5'-deoxy-adenosine

COG id: COG1180

COG function: function code O; Pyruvate-formate lyase-activating enzyme

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the organic radical-activating enzymes family

Homologues:

Organism=Escherichia coli, GI1787130, Length=245, Percent_Identity=45.7142857142857, Blast_Score=244, Evalue=4e-66,
Organism=Escherichia coli, GI1790389, Length=274, Percent_Identity=25.1824817518248, Blast_Score=89, Evalue=3e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PFLA_LISIN (P0A443)

Other databases:

- EMBL:   AL596168
- PIR:   AC1613
- RefSeq:   NP_470780.1
- ProteinModelPortal:   P0A443
- SMR:   P0A443
- GeneID:   1130045
- GenomeReviews:   AL592022_GR
- KEGG:   lin:lin1444
- GenoList:   LIN1444
- HOGENOM:   HBG554871
- OMA:   YLKRIQN
- ProtClustDB:   CLSK564406
- BioCyc:   LINN272626:LIN1444-MONOMER
- BRENDA:   1.97.1.4
- GO:   GO:0005737
- InterPro:   IPR012838
- InterPro:   IPR001989
- InterPro:   IPR007197
- TIGRFAMs:   TIGR02493

Pfam domain/function: PF04055 Radical_SAM

EC number: =1.97.1.4

Molecular weight: Translated: 28116; Mature: 27984

Theoretical pI: Translated: 4.93; Mature: 4.93

Prosite motif: PS01087 RADICAL_ACTIVATING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTEVLGRVHSVETMGTVDGPGIRFIVFMQGCLLRCQFCHNPDTWKIGTGTERSAQDVFDE
CCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCEEECCCCCHHHHHHHHH
AIKYKEFWDASGGGVTVSGGEPLLQVDFLIEFFTLCKAAGVHTTIDSCGGCFTRDPEFIE
HHHHHHHHCCCCCCEEECCCCCHHHHHHHHHHHHHHHHCCCCCCHHHCCCCCCCCHHHHH
KLDRLMEVTDLILLDIKQINPEKHLKLTTKSNAPIIDFAHYLRDKEQPIWIRHVLIPTKT
HHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHCCCCCCEEEEEEEECCCC
DDPEDLTKLHEFIQTLPNVKQVDVLPYHTMGVYKWKEMGIRYPLEGIEAPEEEVVALANK
CCHHHHHHHHHHHHHCCCCCEEECCCCHHHCHHHHHHCCCCCCCCCCCCCHHHHHHHHHH
ILETSSYK
HHHHCCCC
>Mature Secondary Structure 
TEVLGRVHSVETMGTVDGPGIRFIVFMQGCLLRCQFCHNPDTWKIGTGTERSAQDVFDE
CHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCEEECCCCCHHHHHHHHH
AIKYKEFWDASGGGVTVSGGEPLLQVDFLIEFFTLCKAAGVHTTIDSCGGCFTRDPEFIE
HHHHHHHHCCCCCCEEECCCCCHHHHHHHHHHHHHHHHCCCCCCHHHCCCCCCCCHHHHH
KLDRLMEVTDLILLDIKQINPEKHLKLTTKSNAPIIDFAHYLRDKEQPIWIRHVLIPTKT
HHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHCCCCCCEEEEEEEECCCC
DDPEDLTKLHEFIQTLPNVKQVDVLPYHTMGVYKWKEMGIRYPLEGIEAPEEEVVALANK
CCHHHHHHHHHHHHHCCCCCEEECCCCHHHCHHHHHHCCCCCCCCCCCCCHHHHHHHHHH
ILETSSYK
HHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11679669