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The map label for this gene is pflB

Identifier: 226224007

GI number: 226224007

Start: 1419345

End: 1421576

Strand: Direct

Name: pflB

Synonym: Lm4b_01415

Alternate gene names: 226224007

Gene position: 1419345-1421576 (Clockwise)

Preceding gene: 226224006

Following gene: 226224008

Centisome position: 48.73

GC content: 39.16

Gene sequence:

>2232_bases
ATGACTGAACAATGGTATGAATTCGCAGGTGGTAACTGGCAACAAGAAGTAGATGTACGTGACTTTATCTTAAAAAACTA
TCGCTTATATGACGGTGACGACACATTCTTAGTTGGCCCAACCGAAGCAACTACAAAACTTTGGGATCAAGTAATGGACT
TAACTAAAAAAGAACGTGAAAACGGTGGCGTACTGGATATGGATACCAAAATCGTTTCAACCATCACTTCACATGACCCA
GGTTACTTAAACAAAGACTTAGAAAAAGTTGTCGGTGTTCAAACTGACGTACCTTTCAAACGCGCTTTGCAACCATTCGG
TGGAATCCGTATGGCAGAAGTTGCAGCTGAATCTTATGGCTTTAAAGTAGACGAAGAAATTAGTCATATTTTTTCTGAAT
ACCGTAAAACGCATAACCAAGGTGTATTTGATGCTTATACTGCTGAAATGCGCGCAGCTCGTAAATCAGGCGTAATTACT
GGTCTTCCAGATGCTTATGGCCGTGGCCGTATTATCGGTGACTACCGTCGTGTAGCACTTTACGGAGTAGACTTCTTAAT
TAAACAAAAGAAACAAGATTTAAACAATACAGGTTTACGTACAATGAGCGATGACGTTATCCGTCAACGTGAAGAACTTA
ACGAACAAATTCGTGCTCTTGGCGAATTAAAAGTACTAGGCGAAAAACATGGTTTCGATCTTGGTCGCCCAGCTAAAACT
GCACAAGAAGCTTTCCAATGGTTATACCTTGGTTACTTAGCAGCAATTAAAGAACAAAATGGTGCTGCAATGAGCTTAGG
ACGTACATCTACATTCCTTGATATTTATGTAGAACGTGATCTTCGTAACGGCTTAATTACTGAAGAAGAAGCGCAAGAAA
TCGTTGACCACTTCATCATGAAATTACGCCTTGTAAAATTTGCTCGTACACCAGATTACAATGAATTATTCTCTGGAGAT
CCAACATGGGTTACTGAATCCATCGGTGGTATTACAGAAGAAGGCGTTCCACTTGTAACGAAAAACTCATTCCGCTTCTT
ACACACTTTAGATAACTTAGGACCTGCTCCAGAACCAAACTTAACTGTACTTTGGTCCACTCATTTACCATCAGGATTCA
AGAAATTCTGTGCTAAAATGTCTATCAAAACATCTGCTATTCAATACGAAAATGACGATGTTATGCGCCCTAAATGGGGA
GATGACTATGCAATCGCATGTTGTGTATCTGCAATGCGCGTTGGTAAACAAATGCAATTCTTTGGTGCTCGTGCCAACCT
TGCTAAAACACTTCTTTACGCAATCAATGGTGGTATCGATGAAAAATCTAAAGCACAAGTTGGACCTGCTTATCGTCCAG
TTGAAGGCGACGTATTAGACTACAAAGAAGTAATGGAAAAATATGATGCAATGATGGAATGGATTGCAGAACTTTACCTT
AATACATTAAATGTTATTCACTATATGCATGATAAATACGCTTACGAACGTATCGAAATGGCTTTACATGATACAGAAGT
ATTACGTACTATGGCAACTGGTATCGCTGGACTTTCTGTTGCAGCTGACTCCTTATCTGCTATTAAATATGCTACTGTTC
GTCCAATTCGTGACGAAGATGGCATCGTGGTTGATTATGAAATCGAAGGCGACTATCCTAAATACGGAAATAATGATGAC
CGTGTAGACGAAATTGCTGTAGAACTTCTAAAAACATTCATGACTAAAGTTAGAAAACATAAAACTTACCGTGATGCAGT
TCACACAACTTCTGTTCTTACAATCACTTCTAACGTGGTTTATGGTAAGAAAACTGGTAACACTCCAGACGGACGTCGTG
CAGGAGAACCATTTGCACCAGGTGCGAACCCAATGCATGGCCGTGATACAAAAGGTGCTTTAGCTTCTTTATCTTCTGTT
GCTAAACTTCCTTACGAATATGGTCAAGATGGTATTTCTAACACATTCTCTATCGTACCAAAAGCTTTAGGTCGCGAAGA
CGAATCTCAAATCAATAACTTAGTTGCAATGCTAGATGGTTATTCTACAAAAATGGGACATCACTTAAACATCAACGTAT
TCAATCGTGATACATTACTAGATGCGATGGATCATCCAGAAGAGTATCCGCAATTAACTATCCGTGTATCTGGATATGCA
GTTAACTTCATCAAATTAACACGTGAACAACAATTAGATGTTATTCACCGTACAATGCACGAATCCATGTAA

Upstream 100 bases:

>100_bases
ATTTTCCAGAGAATATGTTATGATAACAGTGTAGATTATTTATAAATTAGTTTGCTCAAAAAAGCACGCTCGAGAATGAA
CAATAAATGGAGGTTATATG

Downstream 100 bases:

>100_bases
TAATTAGGCGAGAACGCTTTGGCAAGGATTGCTCACCGTAGCAATCCTTGTTATTTAGGAAAGGAAGAGGAGAATTATGA
CAGAGGTTTTAGGAAGAGTT

Product: pyruvate formate-lyase

Products: NA

Alternate protein names: Pyruvate formate-lyase [H]

Number of amino acids: Translated: 743; Mature: 742

Protein sequence:

>743_residues
MTEQWYEFAGGNWQQEVDVRDFILKNYRLYDGDDTFLVGPTEATTKLWDQVMDLTKKERENGGVLDMDTKIVSTITSHDP
GYLNKDLEKVVGVQTDVPFKRALQPFGGIRMAEVAAESYGFKVDEEISHIFSEYRKTHNQGVFDAYTAEMRAARKSGVIT
GLPDAYGRGRIIGDYRRVALYGVDFLIKQKKQDLNNTGLRTMSDDVIRQREELNEQIRALGELKVLGEKHGFDLGRPAKT
AQEAFQWLYLGYLAAIKEQNGAAMSLGRTSTFLDIYVERDLRNGLITEEEAQEIVDHFIMKLRLVKFARTPDYNELFSGD
PTWVTESIGGITEEGVPLVTKNSFRFLHTLDNLGPAPEPNLTVLWSTHLPSGFKKFCAKMSIKTSAIQYENDDVMRPKWG
DDYAIACCVSAMRVGKQMQFFGARANLAKTLLYAINGGIDEKSKAQVGPAYRPVEGDVLDYKEVMEKYDAMMEWIAELYL
NTLNVIHYMHDKYAYERIEMALHDTEVLRTMATGIAGLSVAADSLSAIKYATVRPIRDEDGIVVDYEIEGDYPKYGNNDD
RVDEIAVELLKTFMTKVRKHKTYRDAVHTTSVLTITSNVVYGKKTGNTPDGRRAGEPFAPGANPMHGRDTKGALASLSSV
AKLPYEYGQDGISNTFSIVPKALGREDESQINNLVAMLDGYSTKMGHHLNINVFNRDTLLDAMDHPEEYPQLTIRVSGYA
VNFIKLTREQQLDVIHRTMHESM

Sequences:

>Translated_743_residues
MTEQWYEFAGGNWQQEVDVRDFILKNYRLYDGDDTFLVGPTEATTKLWDQVMDLTKKERENGGVLDMDTKIVSTITSHDP
GYLNKDLEKVVGVQTDVPFKRALQPFGGIRMAEVAAESYGFKVDEEISHIFSEYRKTHNQGVFDAYTAEMRAARKSGVIT
GLPDAYGRGRIIGDYRRVALYGVDFLIKQKKQDLNNTGLRTMSDDVIRQREELNEQIRALGELKVLGEKHGFDLGRPAKT
AQEAFQWLYLGYLAAIKEQNGAAMSLGRTSTFLDIYVERDLRNGLITEEEAQEIVDHFIMKLRLVKFARTPDYNELFSGD
PTWVTESIGGITEEGVPLVTKNSFRFLHTLDNLGPAPEPNLTVLWSTHLPSGFKKFCAKMSIKTSAIQYENDDVMRPKWG
DDYAIACCVSAMRVGKQMQFFGARANLAKTLLYAINGGIDEKSKAQVGPAYRPVEGDVLDYKEVMEKYDAMMEWIAELYL
NTLNVIHYMHDKYAYERIEMALHDTEVLRTMATGIAGLSVAADSLSAIKYATVRPIRDEDGIVVDYEIEGDYPKYGNNDD
RVDEIAVELLKTFMTKVRKHKTYRDAVHTTSVLTITSNVVYGKKTGNTPDGRRAGEPFAPGANPMHGRDTKGALASLSSV
AKLPYEYGQDGISNTFSIVPKALGREDESQINNLVAMLDGYSTKMGHHLNINVFNRDTLLDAMDHPEEYPQLTIRVSGYA
VNFIKLTREQQLDVIHRTMHESM
>Mature_742_residues
TEQWYEFAGGNWQQEVDVRDFILKNYRLYDGDDTFLVGPTEATTKLWDQVMDLTKKERENGGVLDMDTKIVSTITSHDPG
YLNKDLEKVVGVQTDVPFKRALQPFGGIRMAEVAAESYGFKVDEEISHIFSEYRKTHNQGVFDAYTAEMRAARKSGVITG
LPDAYGRGRIIGDYRRVALYGVDFLIKQKKQDLNNTGLRTMSDDVIRQREELNEQIRALGELKVLGEKHGFDLGRPAKTA
QEAFQWLYLGYLAAIKEQNGAAMSLGRTSTFLDIYVERDLRNGLITEEEAQEIVDHFIMKLRLVKFARTPDYNELFSGDP
TWVTESIGGITEEGVPLVTKNSFRFLHTLDNLGPAPEPNLTVLWSTHLPSGFKKFCAKMSIKTSAIQYENDDVMRPKWGD
DYAIACCVSAMRVGKQMQFFGARANLAKTLLYAINGGIDEKSKAQVGPAYRPVEGDVLDYKEVMEKYDAMMEWIAELYLN
TLNVIHYMHDKYAYERIEMALHDTEVLRTMATGIAGLSVAADSLSAIKYATVRPIRDEDGIVVDYEIEGDYPKYGNNDDR
VDEIAVELLKTFMTKVRKHKTYRDAVHTTSVLTITSNVVYGKKTGNTPDGRRAGEPFAPGANPMHGRDTKGALASLSSVA
KLPYEYGQDGISNTFSIVPKALGREDESQINNLVAMLDGYSTKMGHHLNINVFNRDTLLDAMDHPEEYPQLTIRVSGYAV
NFIKLTREQQLDVIHRTMHESM

Specific function: Glucose metabolism (nonoxidative conversion). [C]

COG id: COG1882

COG function: function code C; Pyruvate-formate lyase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 pyruvate formate lyase domain [H]

Homologues:

Organism=Escherichia coli, GI1787131, Length=752, Percent_Identity=65.8244680851064, Blast_Score=1037, Evalue=0.0,
Organism=Escherichia coli, GI48994926, Length=751, Percent_Identity=61.3848202396804, Blast_Score=982, Evalue=0.0,
Organism=Escherichia coli, GI1787044, Length=574, Percent_Identity=26.8292682926829, Blast_Score=178, Evalue=1e-45,
Organism=Escherichia coli, GI1790388, Length=687, Percent_Identity=25.9097525473071, Blast_Score=166, Evalue=6e-42,
Organism=Escherichia coli, GI1788933, Length=58, Percent_Identity=68.9655172413793, Blast_Score=86, Evalue=9e-18,

Paralogues:

None

Copy number: 3,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005949
- InterPro:   IPR001150
- InterPro:   IPR019777
- InterPro:   IPR004184 [H]

Pfam domain/function: PF01228 Gly_radical; PF02901 PFL [H]

EC number: =2.3.1.54 [H]

Molecular weight: Translated: 83812; Mature: 83681

Theoretical pI: Translated: 5.48; Mature: 5.48

Prosite motif: PS00850 GLY_RADICAL_1 ; PS51149 GLY_RADICAL_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTEQWYEFAGGNWQQEVDVRDFILKNYRLYDGDDTFLVGPTEATTKLWDQVMDLTKKERE
CCCCHHHHCCCCCCCCCCHHHHHHHCCEEECCCCCEEECCCHHHHHHHHHHHHHHHHHHH
NGGVLDMDTKIVSTITSHDPGYLNKDLEKVVGVQTDVPFKRALQPFGGIRMAEVAAESYG
CCCEEEECHHHHHHHHCCCCCCCHHHHHHHHCCCCCCCHHHHHCCCCCCHHHHHHHHHCC
FKVDEEISHIFSEYRKTHNQGVFDAYTAEMRAARKSGVITGLPDAYGRGRIIGDYRRVAL
CCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCEECCCCCCCCCCEEHHHHHHHH
YGVDFLIKQKKQDLNNTGLRTMSDDVIRQREELNEQIRALGELKVLGEKHGFDLGRPAKT
HHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHH
AQEAFQWLYLGYLAAIKEQNGAAMSLGRTSTFLDIYVERDLRNGLITEEEAQEIVDHFIM
HHHHHHHHHHHHHHHHHCCCCCEEECCCCCEEEEEEEEHHHHCCCCCHHHHHHHHHHHHH
KLRLVKFARTPDYNELFSGDPTWVTESIGGITEEGVPLVTKNSFRFLHTLDNLGPAPEPN
HHHHHHHHCCCCHHHHHCCCCCHHHHHHCCCCCCCCCEEECCCHHHHHHHHHCCCCCCCC
LTVLWSTHLPSGFKKFCAKMSIKTSAIQYENDDVMRPKWGDDYAIACCVSAMRVGKQMQF
EEEEEECCCCHHHHHHHHHHHHHHHEEEECCCCCCCCCCCCCHHHHHHHHHHHHCHHHHH
FGARANLAKTLLYAINGGIDEKSKAQVGPAYRPVEGDVLDYKEVMEKYDAMMEWIAELYL
HHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
NTLNVIHYMHDKYAYERIEMALHDTEVLRTMATGIAGLSVAADSLSAIKYATVRPIRDED
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
GIVVDYEIEGDYPKYGNNDDRVDEIAVELLKTFMTKVRKHKTYRDAVHTTSVLTITSNVV
CEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEECCEE
YGKKTGNTPDGRRAGEPFAPGANPMHGRDTKGALASLSSVAKLPYEYGQDGISNTFSIVP
EECCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCHHHCCCCCCHHHHHHH
KALGREDESQINNLVAMLDGYSTKMGHHLNINVFNRDTLLDAMDHPEEYPQLTIRVSGYA
HHHCCCCHHHHHHHHHHHCCCCCCCCCEEEEEEECCHHHHHHHCCCCCCCEEEEEEECEE
VNFIKLTREQQLDVIHRTMHESM
EEEHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TEQWYEFAGGNWQQEVDVRDFILKNYRLYDGDDTFLVGPTEATTKLWDQVMDLTKKERE
CCCHHHHCCCCCCCCCCHHHHHHHCCEEECCCCCEEECCCHHHHHHHHHHHHHHHHHHH
NGGVLDMDTKIVSTITSHDPGYLNKDLEKVVGVQTDVPFKRALQPFGGIRMAEVAAESYG
CCCEEEECHHHHHHHHCCCCCCCHHHHHHHHCCCCCCCHHHHHCCCCCCHHHHHHHHHCC
FKVDEEISHIFSEYRKTHNQGVFDAYTAEMRAARKSGVITGLPDAYGRGRIIGDYRRVAL
CCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCEECCCCCCCCCCEEHHHHHHHH
YGVDFLIKQKKQDLNNTGLRTMSDDVIRQREELNEQIRALGELKVLGEKHGFDLGRPAKT
HHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHH
AQEAFQWLYLGYLAAIKEQNGAAMSLGRTSTFLDIYVERDLRNGLITEEEAQEIVDHFIM
HHHHHHHHHHHHHHHHHCCCCCEEECCCCCEEEEEEEEHHHHCCCCCHHHHHHHHHHHHH
KLRLVKFARTPDYNELFSGDPTWVTESIGGITEEGVPLVTKNSFRFLHTLDNLGPAPEPN
HHHHHHHHCCCCHHHHHCCCCCHHHHHHCCCCCCCCCEEECCCHHHHHHHHHCCCCCCCC
LTVLWSTHLPSGFKKFCAKMSIKTSAIQYENDDVMRPKWGDDYAIACCVSAMRVGKQMQF
EEEEEECCCCHHHHHHHHHHHHHHHEEEECCCCCCCCCCCCCHHHHHHHHHHHHCHHHHH
FGARANLAKTLLYAINGGIDEKSKAQVGPAYRPVEGDVLDYKEVMEKYDAMMEWIAELYL
HHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
NTLNVIHYMHDKYAYERIEMALHDTEVLRTMATGIAGLSVAADSLSAIKYATVRPIRDED
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
GIVVDYEIEGDYPKYGNNDDRVDEIAVELLKTFMTKVRKHKTYRDAVHTTSVLTITSNVV
CEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEECCEE
YGKKTGNTPDGRRAGEPFAPGANPMHGRDTKGALASLSSVAKLPYEYGQDGISNTFSIVP
EECCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCHHHCCCCCCHHHHHHH
KALGREDESQINNLVAMLDGYSTKMGHHLNINVFNRDTLLDAMDHPEEYPQLTIRVSGYA
HHHCCCCHHHHHHHHHHHCCCCCCCCCEEEEEEECCHHHHHHHCCCCCCCEEEEEEECEE
VNFIKLTREQQLDVIHRTMHESM
EEEHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA