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The map label for this gene is ispA [C]

Identifier: 226223964

GI number: 226223964

Start: 1369641

End: 1370522

Strand: Direct

Name: ispA [C]

Synonym: Lm4b_01371

Alternate gene names: 226223964

Gene position: 1369641-1370522 (Clockwise)

Preceding gene: 226223963

Following gene: 226223965

Centisome position: 47.02

GC content: 38.21

Gene sequence:

>882_bases
TTGCAAGATTTAACCCTTTTTCTAGAACAATATAAAAAAGTGATTGATGAGTCGCTTTTTAAAGAAATAAGCGAGCGAAA
TATCGAACCTAGACTAAAAGAGTCCATGTTATATTCTGTTCAAGCAGGTGGAAAACGGATTCGTCCCATGCTAGTTTTTG
CTACACTTCAAGCCTTAAAAGTAAATCCGCTTTTAGGTGTGAAAACAGCAACGGCGTTAGAAATGATTCATACGTATAGC
CTGATCCATGATGATTTACCAGCAATGGATAATGATGACTATCGTCGCGGCAAATATACTAATCATAAAGTTTTTGGGGA
TGCAACTGCGATTTTGGCAGGAGACGCTTTACTAACGCTTGCTTTTTCTATTTTAGCGGAAGACGAAAATTTATCTTTTG
AAACACGCATAGCTTTGATTAATCAAATTAGTTTTAGTAGTGGTGCAGAAGGAATGGTTGGCGGTCAACTTGCAGATATG
GAGGCAGAAAACAAACAAGTGACATTAGAAGAACTATCATCCATCCATGCACGGAAAACAGGCGAATTATTAATTTTTGC
CGTAACTTCTGCTGCGAAAATTGCAGAAGCTGACCCGGAACAAACGAAAAGATTACGGATTTTTGCGGAAAATATTGGGA
TTGGATTCCAAATCAGCGACGATATTTTAGATGTTATTGGTGATGAAACAAAAATGGGTAAAAAGACAGGGGTCGACGCT
TTTCTGAACAAAAGTACCTATCCCGGATTACTCACGCTTGATGGTGCCAAAAGGGCATTAAATGAGCATGTTGCGATTGC
AAAGTCAGCGCTTTCAGGGCATGATTTTGATGATGAAATTCTCTTGAAACTTGCTGATTTAATCGCACTTAGAGAAAATT
AA

Upstream 100 bases:

>100_bases
AATTGTGCCAAGATAAATTACAATCAGCAGAAAAACGAATGGCAAAAGTAGTCACAGATGCAGGAGAAGAAATTCCTTTT
GAAGCGGATGGTGAATAAAT

Downstream 100 bases:

>100_bases
TCATAATTATCTAGTAATTTCAAATTTTTTTCACATATATAATTCAAATTGATTTGCTTTTCCTAAAATACCGTGTTATA
CTAATGTAAGATTATTTTTG

Product: Trans-Isoprenyl Diphosphate Synthase

Products: NA

Alternate protein names: FPP synthase; (2E,6E)-farnesyl diphosphate synthase; Geranyltranstransferase [H]

Number of amino acids: Translated: 293; Mature: 293

Protein sequence:

>293_residues
MQDLTLFLEQYKKVIDESLFKEISERNIEPRLKESMLYSVQAGGKRIRPMLVFATLQALKVNPLLGVKTATALEMIHTYS
LIHDDLPAMDNDDYRRGKYTNHKVFGDATAILAGDALLTLAFSILAEDENLSFETRIALINQISFSSGAEGMVGGQLADM
EAENKQVTLEELSSIHARKTGELLIFAVTSAAKIAEADPEQTKRLRIFAENIGIGFQISDDILDVIGDETKMGKKTGVDA
FLNKSTYPGLLTLDGAKRALNEHVAIAKSALSGHDFDDEILLKLADLIALREN

Sequences:

>Translated_293_residues
MQDLTLFLEQYKKVIDESLFKEISERNIEPRLKESMLYSVQAGGKRIRPMLVFATLQALKVNPLLGVKTATALEMIHTYS
LIHDDLPAMDNDDYRRGKYTNHKVFGDATAILAGDALLTLAFSILAEDENLSFETRIALINQISFSSGAEGMVGGQLADM
EAENKQVTLEELSSIHARKTGELLIFAVTSAAKIAEADPEQTKRLRIFAENIGIGFQISDDILDVIGDETKMGKKTGVDA
FLNKSTYPGLLTLDGAKRALNEHVAIAKSALSGHDFDDEILLKLADLIALREN
>Mature_293_residues
MQDLTLFLEQYKKVIDESLFKEISERNIEPRLKESMLYSVQAGGKRIRPMLVFATLQALKVNPLLGVKTATALEMIHTYS
LIHDDLPAMDNDDYRRGKYTNHKVFGDATAILAGDALLTLAFSILAEDENLSFETRIALINQISFSSGAEGMVGGQLADM
EAENKQVTLEELSSIHARKTGELLIFAVTSAAKIAEADPEQTKRLRIFAENIGIGFQISDDILDVIGDETKMGKKTGVDA
FLNKSTYPGLLTLDGAKRALNEHVAIAKSALSGHDFDDEILLKLADLIALREN

Specific function: Unknown

COG id: COG0142

COG function: function code H; Geranylgeranyl pyrophosphate synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FPP/GGPP synthase family [H]

Homologues:

Organism=Homo sapiens, GI50659086, Length=234, Percent_Identity=32.9059829059829, Blast_Score=96, Evalue=3e-20,
Organism=Escherichia coli, GI1786623, Length=267, Percent_Identity=46.0674157303371, Blast_Score=180, Evalue=8e-47,
Organism=Escherichia coli, GI1789578, Length=212, Percent_Identity=34.4339622641509, Blast_Score=106, Evalue=2e-24,
Organism=Caenorhabditis elegans, GI17505681, Length=188, Percent_Identity=31.9148936170213, Blast_Score=76, Evalue=2e-14,
Organism=Drosophila melanogaster, GI24651612, Length=237, Percent_Identity=29.957805907173, Blast_Score=89, Evalue=3e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000092
- InterPro:   IPR017446
- InterPro:   IPR008949 [H]

Pfam domain/function: PF00348 polyprenyl_synt [H]

EC number: =2.5.1.10 [H]

Molecular weight: Translated: 32258; Mature: 32258

Theoretical pI: Translated: 4.74; Mature: 4.74

Prosite motif: PS00052 RIBOSOMAL_S7 ; PS00723 POLYPRENYL_SYNTHET_1 ; PS00444 POLYPRENYL_SYNTHET_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQDLTLFLEQYKKVIDESLFKEISERNIEPRLKESMLYSVQAGGKRIRPMLVFATLQALK
CCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
VNPLLGVKTATALEMIHTYSLIHDDLPAMDNDDYRRGKYTNHKVFGDATAILAGDALLTL
CCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEECCCHHHHHHHHHHHHH
AFSILAEDENLSFETRIALINQISFSSGAEGMVGGQLADMEAENKQVTLEELSSIHARKT
HHHHHCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCEEECCCCCCCEEEHHHHHHHHCCCC
GELLIFAVTSAAKIAEADPEQTKRLRIFAENIGIGFQISDDILDVIGDETKMGKKTGVDA
CCEEEEEECCCHHHHCCCHHHHHHEEEEEHHCCCCEEECHHHHHHHCCCHHCCCCCCHHH
FLNKSTYPGLLTLDGAKRALNEHVAIAKSALSGHDFDDEILLKLADLIALREN
HHCCCCCCCEEEECCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MQDLTLFLEQYKKVIDESLFKEISERNIEPRLKESMLYSVQAGGKRIRPMLVFATLQALK
CCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
VNPLLGVKTATALEMIHTYSLIHDDLPAMDNDDYRRGKYTNHKVFGDATAILAGDALLTL
CCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEECCCHHHHHHHHHHHHH
AFSILAEDENLSFETRIALINQISFSSGAEGMVGGQLADMEAENKQVTLEELSSIHARKT
HHHHHCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCEEECCCCCCCEEEHHHHHHHHCCCC
GELLIFAVTSAAKIAEADPEQTKRLRIFAENIGIGFQISDDILDVIGDETKMGKKTGVDA
CCEEEEEECCCHHHHCCCHHHHHHEEEEEHHCCCCEEECHHHHHHHCCCHHCCCCCCHHH
FLNKSTYPGLLTLDGAKRALNEHVAIAKSALSGHDFDDEILLKLADLIALREN
HHCCCCCCCEEEECCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8486607; 7918490 [H]