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The map label for this gene is miaA

Identifier: 226223896

GI number: 226223896

Start: 1306959

End: 1307876

Strand: Direct

Name: miaA

Synonym: Lm4b_01303

Alternate gene names: 226223896

Gene position: 1306959-1307876 (Clockwise)

Preceding gene: 226223895

Following gene: 226223897

Centisome position: 44.87

GC content: 37.25

Gene sequence:

>918_bases
TTGAGCAAGATTCCTGTCATCGTCATCGTTGGCCCAACCGCTGTCGGAAAGACAAGTTTAAGTATTGAACTAGCGAAAAA
ATTGGACGGAGAAATCATCAGTGGTGACTCTATGCAAGTTTATCGTGGATTAGATATCGGTACAGCAAAAATCACACCAG
AAGAAATGGATGGAATCAAACATTATCTTATTGATGTGACGAATCCTTCCGAACCATTCACTGCAGCGAAATTCCAAATA
GAAACAAGAAAATGTATAGAAACAATACATCAAGCTGGTAGGCTACCAATTATTGTTGGTGGAACAGGACTTTATATTCA
ATCTGTTTTCTATGATTATGATTTTGGTAACGTAAGCGAAGATAAGGCTTATCGAGCGGAATTAGAACAACTAGACAAAA
CTGTATTGTGGCAAATGCTCGAACAAAAAGATCCAGAAAGCGCAGCACAGATACACGAAAACAATAAGCGCCGAGTGATT
AGAGCATTAGAAGTGATGCATCTAACAGGAAAGCCTTTCTCAGAGTATCAAGTGAATAATGTACTTAATGATAGATACCA
ACCACTCTTTCTAGGACTTGATTTGGACAGAGCGTTACTTTACGAAAGAATTAATCAGCGAGTGAATTTAATGTTTGAAG
AAGGTTTAGTGACAGAAGCAAAAAAATTATATGAGCAACATTTAGTGGATGTTCCAGCAGTTCGCGGCATCGGCTACAAA
GAATTATTTCCTTATTTTGAAGGAAAGAGCTCACTAGAAGAAGCAAAAGAATTAATTCAAAAGAACTCAAGGCATTTCGC
GAAAAGACAGTTAACCTGGTTTAGAAATAGAATGGATATTGACTGGATTCAGGCGGGTGTTAGCAGTACAGAATCAGAAG
CATTAAATAAAGCAACAACCTTTTTAACAGCTAAATAA

Upstream 100 bases:

>100_bases
TTCAAGGATTTGCGCTTTTTTTCTGGAAAAAATCATTTTCACTATTTTCAAGAAACCTTTTTCATGGTACATTGATTACA
GGTGAAAAGGAGGTTTTTTT

Downstream 100 bases:

>100_bases
CGCTTTTCTGTTTGGTAAGAAGAAATAAAGGGTATTTGAAATTAGCCAACCAATTGCACAATGGAACTTCATTGGGGTTT
GGGAAATTTTTAGTATAAGA

Product: tRNA delta(2)-isopentenylpyrophosphate transferase

Products: NA

Alternate protein names: Dimethylallyl diphosphate:tRNA dimethylallyltransferase; DMAPP:tRNA dimethylallyltransferase; DMATase; Isopentenyl-diphosphate:tRNA isopentenyltransferase; IPP transferase; IPPT; IPTase

Number of amino acids: Translated: 305; Mature: 304

Protein sequence:

>305_residues
MSKIPVIVIVGPTAVGKTSLSIELAKKLDGEIISGDSMQVYRGLDIGTAKITPEEMDGIKHYLIDVTNPSEPFTAAKFQI
ETRKCIETIHQAGRLPIIVGGTGLYIQSVFYDYDFGNVSEDKAYRAELEQLDKTVLWQMLEQKDPESAAQIHENNKRRVI
RALEVMHLTGKPFSEYQVNNVLNDRYQPLFLGLDLDRALLYERINQRVNLMFEEGLVTEAKKLYEQHLVDVPAVRGIGYK
ELFPYFEGKSSLEEAKELIQKNSRHFAKRQLTWFRNRMDIDWIQAGVSSTESEALNKATTFLTAK

Sequences:

>Translated_305_residues
MSKIPVIVIVGPTAVGKTSLSIELAKKLDGEIISGDSMQVYRGLDIGTAKITPEEMDGIKHYLIDVTNPSEPFTAAKFQI
ETRKCIETIHQAGRLPIIVGGTGLYIQSVFYDYDFGNVSEDKAYRAELEQLDKTVLWQMLEQKDPESAAQIHENNKRRVI
RALEVMHLTGKPFSEYQVNNVLNDRYQPLFLGLDLDRALLYERINQRVNLMFEEGLVTEAKKLYEQHLVDVPAVRGIGYK
ELFPYFEGKSSLEEAKELIQKNSRHFAKRQLTWFRNRMDIDWIQAGVSSTESEALNKATTFLTAK
>Mature_304_residues
SKIPVIVIVGPTAVGKTSLSIELAKKLDGEIISGDSMQVYRGLDIGTAKITPEEMDGIKHYLIDVTNPSEPFTAAKFQIE
TRKCIETIHQAGRLPIIVGGTGLYIQSVFYDYDFGNVSEDKAYRAELEQLDKTVLWQMLEQKDPESAAQIHENNKRRVIR
ALEVMHLTGKPFSEYQVNNVLNDRYQPLFLGLDLDRALLYERINQRVNLMFEEGLVTEAKKLYEQHLVDVPAVRGIGYKE
LFPYFEGKSSLEEAKELIQKNSRHFAKRQLTWFRNRMDIDWIQAGVSSTESEALNKATTFLTAK

Specific function: Catalyzes the transfer of a dimethylallyl group onto the adenine at position 37 in tRNAs that read codons beginning with uridine, leading to the formation of N6-(dimethylallyl)adenosine (i(6)A)

COG id: COG0324

COG function: function code J; tRNA delta(2)-isopentenylpyrophosphate transferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the IPP transferase family

Homologues:

Organism=Homo sapiens, GI31581534, Length=311, Percent_Identity=32.7974276527331, Blast_Score=161, Evalue=8e-40,
Organism=Escherichia coli, GI1790613, Length=286, Percent_Identity=37.0629370629371, Blast_Score=181, Evalue=7e-47,
Organism=Caenorhabditis elegans, GI25144712, Length=274, Percent_Identity=33.9416058394161, Blast_Score=136, Evalue=2e-32,
Organism=Saccharomyces cerevisiae, GI6324848, Length=291, Percent_Identity=37.4570446735395, Blast_Score=181, Evalue=9e-47,
Organism=Drosophila melanogaster, GI24649830, Length=340, Percent_Identity=33.5294117647059, Blast_Score=172, Evalue=2e-43,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MIAA_LISMC (C1L2K1)

Other databases:

- EMBL:   FM242711
- RefSeq:   YP_002758003.1
- ProteinModelPortal:   C1L2K1
- SMR:   C1L2K1
- GeneID:   7702938
- GenomeReviews:   FM242711_GR
- KEGG:   lmc:Lm4b_01303
- OMA:   VNADSMQ
- ProtClustDB:   PRK00091
- HAMAP:   MF_00185
- InterPro:   IPR002627
- InterPro:   IPR018022
- PANTHER:   PTHR11088
- TIGRFAMs:   TIGR00174

Pfam domain/function: PF01715 IPPT

EC number: =2.5.1.75

Molecular weight: Translated: 34749; Mature: 34618

Theoretical pI: Translated: 6.00; Mature: 6.00

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKIPVIVIVGPTAVGKTSLSIELAKKLDGEIISGDSMQVYRGLDIGTAKITPEEMDGIK
CCCCCEEEEECCCCCCCCHHHHHHHHHCCCCEECCCCEEEEECCCCCCCCCCHHHHCCCE
HYLIDVTNPSEPFTAAKFQIETRKCIETIHQAGRLPIIVGGTGLYIQSVFYDYDFGNVSE
EEEEECCCCCCCCEEEEEEEHHHHHHHHHHHCCCCEEEECCCHHHHHHHHHCCCCCCCCC
DKAYRAELEQLDKTVLWQMLEQKDPESAAQIHENNKRRVIRALEVMHLTGKPFSEYQVNN
HHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCHHHHHHHHHHHHHCCCCHHHHHHHH
VLNDRYQPLFLGLDLDRALLYERINQRVNLMFEEGLVTEAKKLYEQHLVDVPAVRGIGYK
HHCCCCCEEEEECCHHHHHHHHHHHHHHEEHHHCCHHHHHHHHHHHHHCCCCCCCCCCHH
ELFPYFEGKSSLEEAKELIQKNSRHFAKRQLTWFRNRMDIDWIQAGVSSTESEALNKATT
HHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCHHHHHHHHHHH
FLTAK
EEECC
>Mature Secondary Structure 
SKIPVIVIVGPTAVGKTSLSIELAKKLDGEIISGDSMQVYRGLDIGTAKITPEEMDGIK
CCCCEEEEECCCCCCCCHHHHHHHHHCCCCEECCCCEEEEECCCCCCCCCCHHHHCCCE
HYLIDVTNPSEPFTAAKFQIETRKCIETIHQAGRLPIIVGGTGLYIQSVFYDYDFGNVSE
EEEEECCCCCCCCEEEEEEEHHHHHHHHHHHCCCCEEEECCCHHHHHHHHHCCCCCCCCC
DKAYRAELEQLDKTVLWQMLEQKDPESAAQIHENNKRRVIRALEVMHLTGKPFSEYQVNN
HHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCHHHHHHHHHHHHHCCCCHHHHHHHH
VLNDRYQPLFLGLDLDRALLYERINQRVNLMFEEGLVTEAKKLYEQHLVDVPAVRGIGYK
HHCCCCCEEEEECCHHHHHHHHHHHHHHEEHHHCCHHHHHHHHHHHHHCCCCCCCCCCHH
ELFPYFEGKSSLEEAKELIQKNSRHFAKRQLTWFRNRMDIDWIQAGVSSTESEALNKATT
HHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCHHHHHHHHHHH
FLTAK
EEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA