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The map label for this gene is lmrB [H]

Identifier: 226223144

GI number: 226223144

Start: 549795

End: 551210

Strand: Direct

Name: lmrB [H]

Synonym: Lm4b_00541

Alternate gene names: 226223144

Gene position: 549795-551210 (Clockwise)

Preceding gene: 226223140

Following gene: 226223145

Centisome position: 18.88

GC content: 41.31

Gene sequence:

>1416_bases
ATGCAACAAGAAGCAACAGGTGGGCAGAAAATTCGGCCGATACCGATTATTGCCTCATTTTTGATGGCGGGGTTCATTGG
GCTATTCAGTGAAACTGCTCTTAACATGGCGCTTAGTGATTTGATTCAGGTGTTTGATATTAGTTCAGCGACAGTGCAGT
GGCTTACGACAGGTTATTTGCTAACGCTTGGAATATTAGTACCGATTTCGGGATTACTTTTACAATGGTTTACAACACGA
GGTTTATTTTTTACAGCAGTGAGTTTTTCGATTGCTGGTACGCTGATTGCGGCGCTTTCGCCAACGTTTGCAATGTTAAT
GATTGGGCGTGTAGTGCAAGCAGTAGGTACGGCGCTATTACTACCATTAATGTTTAACACGATTTTACTGATTTTCCCAG
AGCATAAACGTGGCTCAGCAATGGGGATGATCGGGCTGGTAATTATGTTTGCTCCAGCAGTTGGTCCGACAATTTCAGGA
CTAATTTTAGAAAACTTAACTTGGAACTGGATTTTCTGGATTTCCTTGCCATTCCTTATTATTGCGTTATTATTCGGAAT
GAAATTTATGCAAAATGTTTCGGTTGTTACGAAGCCGAAAATTGATATTTTATCGATTATTCTTTCGACGCTAGGTTTTG
GTGGAGTTGTATTTGCCTTTAGTAGTGCGGGAGAAAGTGGTTGGGGAAGCGCAACGGTATTAGTTTCAATTATCGTTGGT
GGACTTGCGCTTGGACTCTTTGTTTGGCGCCAACTAACAATGGAAAAACCTTTGATGGACTTGAAAGTATTTAAATACCC
AATGTTCACATTAGGACTTATTTTAGTATTTATCAGCTTTATGATGATTCTTTCGACGATGATTTTACTACCGCTATACT
TGCAAAATAGTTTAGCGCTCGCAGCATTTTCAGCAGGATTAGTATTACTTCCGGGTGGGGTGCTGAATGGTTTAATGTCA
CCATTTACTGGCCGTTTGTTCGATGCATACGGTCCACGCGCACTTGTTATCCCAGGGTTTATCGTAGCGGTTGTGGCACT
ATTTTTCTTAACGAGAATAGAAGTTGGAACATCTGCATTAACCATCATCGTACTTCATTCGGTGTTAATGATTGGGATTT
CGATGGTCATGATGCCGGCACAAACAAACGGATTAAACCAATTACCGCCAAAATTATATCCTGATGGTACGGCGATTATG
AACACGTTGCAACAAGTTTCCGGCGCGATTGGAACGGCTGTTGCGATTACAATCATGTCAGCTGGACAAAAAGCTTATAT
GGAAACGGCGCAAGGAGTAGGACCAGAGCAAATGGTTGCTTCACTGACAGCAGGAATTCAAAATGCCTTTGTCTTTGGAC
TGATTATGGCTTGTATTGGTCTTCTGTGCTCGTTATTTATTCGTAAAGCTAAATAA

Upstream 100 bases:

>100_bases
TTTTCGTAAAAATAGAGCTTGAAACGTTGACTTATTGGCGAAATCTCCGTAATATCAAACGTAGACTACTTAATTTTTAG
TCTGAAAAGGGGGAAACTAT

Downstream 100 bases:

>100_bases
TAAAGCGAAGAGAACTTCTCGTAATGAGAGGTTCTTTTTTGTTTTGACAAATGGGTTCTGAGTGGTTAAAATGATAGTAA
ATATAGTTTATAAAGTTTGT

Product: multidrug resistance protein

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 471; Mature: 471

Protein sequence:

>471_residues
MQQEATGGQKIRPIPIIASFLMAGFIGLFSETALNMALSDLIQVFDISSATVQWLTTGYLLTLGILVPISGLLLQWFTTR
GLFFTAVSFSIAGTLIAALSPTFAMLMIGRVVQAVGTALLLPLMFNTILLIFPEHKRGSAMGMIGLVIMFAPAVGPTISG
LILENLTWNWIFWISLPFLIIALLFGMKFMQNVSVVTKPKIDILSIILSTLGFGGVVFAFSSAGESGWGSATVLVSIIVG
GLALGLFVWRQLTMEKPLMDLKVFKYPMFTLGLILVFISFMMILSTMILLPLYLQNSLALAAFSAGLVLLPGGVLNGLMS
PFTGRLFDAYGPRALVIPGFIVAVVALFFLTRIEVGTSALTIIVLHSVLMIGISMVMMPAQTNGLNQLPPKLYPDGTAIM
NTLQQVSGAIGTAVAITIMSAGQKAYMETAQGVGPEQMVASLTAGIQNAFVFGLIMACIGLLCSLFIRKAK

Sequences:

>Translated_471_residues
MQQEATGGQKIRPIPIIASFLMAGFIGLFSETALNMALSDLIQVFDISSATVQWLTTGYLLTLGILVPISGLLLQWFTTR
GLFFTAVSFSIAGTLIAALSPTFAMLMIGRVVQAVGTALLLPLMFNTILLIFPEHKRGSAMGMIGLVIMFAPAVGPTISG
LILENLTWNWIFWISLPFLIIALLFGMKFMQNVSVVTKPKIDILSIILSTLGFGGVVFAFSSAGESGWGSATVLVSIIVG
GLALGLFVWRQLTMEKPLMDLKVFKYPMFTLGLILVFISFMMILSTMILLPLYLQNSLALAAFSAGLVLLPGGVLNGLMS
PFTGRLFDAYGPRALVIPGFIVAVVALFFLTRIEVGTSALTIIVLHSVLMIGISMVMMPAQTNGLNQLPPKLYPDGTAIM
NTLQQVSGAIGTAVAITIMSAGQKAYMETAQGVGPEQMVASLTAGIQNAFVFGLIMACIGLLCSLFIRKAK
>Mature_471_residues
MQQEATGGQKIRPIPIIASFLMAGFIGLFSETALNMALSDLIQVFDISSATVQWLTTGYLLTLGILVPISGLLLQWFTTR
GLFFTAVSFSIAGTLIAALSPTFAMLMIGRVVQAVGTALLLPLMFNTILLIFPEHKRGSAMGMIGLVIMFAPAVGPTISG
LILENLTWNWIFWISLPFLIIALLFGMKFMQNVSVVTKPKIDILSIILSTLGFGGVVFAFSSAGESGWGSATVLVSIIVG
GLALGLFVWRQLTMEKPLMDLKVFKYPMFTLGLILVFISFMMILSTMILLPLYLQNSLALAAFSAGLVLLPGGVLNGLMS
PFTGRLFDAYGPRALVIPGFIVAVVALFFLTRIEVGTSALTIIVLHSVLMIGISMVMMPAQTNGLNQLPPKLYPDGTAIM
NTLQQVSGAIGTAVAITIMSAGQKAYMETAQGVGPEQMVASLTAGIQNAFVFGLIMACIGLLCSLFIRKAK

Specific function: Proton-dependent transporter. May mediate the efflux of lincomycin [H]

COG id: COG0477

COG function: function code GEPR; Permeases of the major facilitator superfamily

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. EmrB family [H]

Homologues:

Organism=Escherichia coli, GI1788710, Length=414, Percent_Identity=26.5700483091787, Blast_Score=150, Evalue=2e-37,
Organism=Escherichia coli, GI1789042, Length=438, Percent_Identity=25.5707762557078, Blast_Score=136, Evalue=2e-33,
Organism=Escherichia coli, GI87081983, Length=332, Percent_Identity=29.8192771084337, Blast_Score=132, Evalue=4e-32,
Organism=Escherichia coli, GI1790195, Length=417, Percent_Identity=26.6187050359712, Blast_Score=118, Evalue=9e-28,
Organism=Escherichia coli, GI1788392, Length=394, Percent_Identity=28.4263959390863, Blast_Score=103, Evalue=2e-23,
Organism=Saccharomyces cerevisiae, GI6325455, Length=246, Percent_Identity=28.4552845528455, Blast_Score=74, Evalue=4e-14,
Organism=Saccharomyces cerevisiae, GI6322958, Length=444, Percent_Identity=23.4234234234234, Blast_Score=66, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004638
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR001411 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 50314; Mature: 50314

Theoretical pI: Translated: 9.81; Mature: 9.81

Prosite motif: PS00639 THIOL_PROTEASE_HIS ; PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
5.7 %Met     (Translated Protein)
6.2 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
5.7 %Met     (Mature Protein)
6.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQQEATGGQKIRPIPIIASFLMAGFIGLFSETALNMALSDLIQVFDISSATVQWLTTGYL
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
LTLGILVPISGLLLQWFTTRGLFFTAVSFSIAGTLIAALSPTFAMLMIGRVVQAVGTALL
HHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LPLMFNTILLIFPEHKRGSAMGMIGLVIMFAPAVGPTISGLILENLTWNWIFWISLPFLI
HHHHHHHHEEEECCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHCCCCHHHHHHHHHHHH
IALLFGMKFMQNVSVVTKPKIDILSIILSTLGFGGVVFAFSSAGESGWGSATVLVSIIVG
HHHHHHHHHHHCCCHHCCCCHHHHHHHHHHHCCCHHHHHHHCCCCCCCCHHHHHHHHHHH
GLALGLFVWRQLTMEKPLMDLKVFKYPMFTLGLILVFISFMMILSTMILLPLYLQNSLAL
HHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AAFSAGLVLLPGGVLNGLMSPFTGRLFDAYGPRALVIPGFIVAVVALFFLTRIEVGTSAL
HHHHCCHHEECCHHHHHHHCHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHHHHHCHHHH
TIIVLHSVLMIGISMVMMPAQTNGLNQLPPKLYPDGTAIMNTLQQVSGAIGTAVAITIMS
HHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
AGQKAYMETAQGVGPEQMVASLTAGIQNAFVFGLIMACIGLLCSLFIRKAK
CCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MQQEATGGQKIRPIPIIASFLMAGFIGLFSETALNMALSDLIQVFDISSATVQWLTTGYL
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
LTLGILVPISGLLLQWFTTRGLFFTAVSFSIAGTLIAALSPTFAMLMIGRVVQAVGTALL
HHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LPLMFNTILLIFPEHKRGSAMGMIGLVIMFAPAVGPTISGLILENLTWNWIFWISLPFLI
HHHHHHHHEEEECCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHCCCCHHHHHHHHHHHH
IALLFGMKFMQNVSVVTKPKIDILSIILSTLGFGGVVFAFSSAGESGWGSATVLVSIIVG
HHHHHHHHHHHCCCHHCCCCHHHHHHHHHHHCCCHHHHHHHCCCCCCCCHHHHHHHHHHH
GLALGLFVWRQLTMEKPLMDLKVFKYPMFTLGLILVFISFMMILSTMILLPLYLQNSLAL
HHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AAFSAGLVLLPGGVLNGLMSPFTGRLFDAYGPRALVIPGFIVAVVALFFLTRIEVGTSAL
HHHHCCHHEECCHHHHHHHCHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHHHHHCHHHH
TIIVLHSVLMIGISMVMMPAQTNGLNQLPPKLYPDGTAIMNTLQQVSGAIGTAVAITIMS
HHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
AGQKAYMETAQGVGPEQMVASLTAGIQNAFVFGLIMACIGLLCSLFIRKAK
CCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11679669 [H]