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The map label for this gene is yofA [H]

Identifier: 226222930

GI number: 226222930

Start: 327322

End: 328185

Strand: Reverse

Name: yofA [H]

Synonym: Lm4b_00322

Alternate gene names: 226222930

Gene position: 328185-327322 (Counterclockwise)

Preceding gene: 226222933

Following gene: 226222921

Centisome position: 11.27

GC content: 33.68

Gene sequence:

>864_bases
ATGGATATTGAAAACATGAAAGCATTTAATAAAGTAGCTGAACTAAAGAGTATTTCTGCAGCAGCGAACGAACTGCATCA
TTTACAGTCTAATATGTCCAATAAAATAAAAAATATCGAAAAACAGTTCCAAACTCAACTTTTCTTCCGGCACTCGAATG
GTGTGGAACCAACTAAAGAGGGCGAAAAAATCTATCAACAATTCAAAAAAATGATTTTATTATGGGAGGAAACGATTGAT
ATTATCAATAATGAAGAAGAAACTATCTCAATCGGTATTACGCAATCTTCTTTGCCTATGGAATTTAACACAATCATTAA
AGAGTTCTATCAACAGTTTCCAAATAAAAAATTATCTATCGTTAGCGGAAGTACAAGTGAATTAATACCAAAAATAGCTA
ACAGAGAACTAACCATCGCATATGTCGCTGAACTCGAAAAAGAAAATTTATTTCAAGATAGCCAAATCATCTCACAAACG
CTCTCATGGGATAAACTAGTTTTCGCAGGCAACACAGCTGGAAAATCAGTCCAAAAAATATTAGCGGAAGAACGATTATA
CGTCTTCAGCAAACAATGTTATTCATACCGCGCCCTGGCTGCTCTCATTAACGATATTAATATCCCAAATGTTTCCATAT
CAGAGATCAATATTCCCGAAACACTTGTTGAAATCTGCAATAATGAACTTGGTATTGGAATTATCCCCGAAAGCATCGCA
CTAAACTATCATTTTCTAAACTATGAAACATTACCCACAGAATACGCATCACTTCGAAAAACATTAATTTATCATGCAGA
TCATACAATTTCAAATGGGGAAAAATGGCTTATCGAAAAAAGTAAACCAGCATTCAAAAGTTAA

Upstream 100 bases:

>100_bases
AATTTATATTGTAAATAAAGTATAATCTATCTGCGCTATCATTTGAAATGCGAAAATCATATGCCGGTATCATTATTTTT
GATACCAAAAGGAGAAAATT

Downstream 100 bases:

>100_bases
GTTGTTGATTACAACCCTTTCAACCAGCTAGCTATTTATGATAAGGCTCCCCCCGAACAATTCGAAACGCCCGGTAAACC
TGCTCCACCAGCACCAATCT

Product: transcription regulator LysR-gltR family

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 287; Mature: 287

Protein sequence:

>287_residues
MDIENMKAFNKVAELKSISAAANELHHLQSNMSNKIKNIEKQFQTQLFFRHSNGVEPTKEGEKIYQQFKKMILLWEETID
IINNEEETISIGITQSSLPMEFNTIIKEFYQQFPNKKLSIVSGSTSELIPKIANRELTIAYVAELEKENLFQDSQIISQT
LSWDKLVFAGNTAGKSVQKILAEERLYVFSKQCYSYRALAALINDINIPNVSISEINIPETLVEICNNELGIGIIPESIA
LNYHFLNYETLPTEYASLRKTLIYHADHTISNGEKWLIEKSKPAFKS

Sequences:

>Translated_287_residues
MDIENMKAFNKVAELKSISAAANELHHLQSNMSNKIKNIEKQFQTQLFFRHSNGVEPTKEGEKIYQQFKKMILLWEETID
IINNEEETISIGITQSSLPMEFNTIIKEFYQQFPNKKLSIVSGSTSELIPKIANRELTIAYVAELEKENLFQDSQIISQT
LSWDKLVFAGNTAGKSVQKILAEERLYVFSKQCYSYRALAALINDINIPNVSISEINIPETLVEICNNELGIGIIPESIA
LNYHFLNYETLPTEYASLRKTLIYHADHTISNGEKWLIEKSKPAFKS
>Mature_287_residues
MDIENMKAFNKVAELKSISAAANELHHLQSNMSNKIKNIEKQFQTQLFFRHSNGVEPTKEGEKIYQQFKKMILLWEETID
IINNEEETISIGITQSSLPMEFNTIIKEFYQQFPNKKLSIVSGSTSELIPKIANRELTIAYVAELEKENLFQDSQIISQT
LSWDKLVFAGNTAGKSVQKILAEERLYVFSKQCYSYRALAALINDINIPNVSISEINIPETLVEICNNELGIGIIPESIA
LNYHFLNYETLPTEYASLRKTLIYHADHTISNGEKWLIEKSKPAFKS

Specific function: Regulates expression of the cell division protein ftsW, and is essential for cell viability during stationary phase [H]

COG id: COG0583

COG function: function code K; Transcriptional regulator

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787806, Length=247, Percent_Identity=18.6234817813765, Blast_Score=70, Evalue=2e-13,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 32802; Mature: 32802

Theoretical pI: Translated: 5.44; Mature: 5.44

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDIENMKAFNKVAELKSISAAANELHHLQSNMSNKIKNIEKQFQTQLFFRHSNGVEPTKE
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHH
GEKIYQQFKKMILLWEETIDIINNEEETISIGITQSSLPMEFNTIIKEFYQQFPNKKLSI
HHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCHHHHHHHHHHHHHCCCCEEEE
VSGSTSELIPKIANRELTIAYVAELEKENLFQDSQIISQTLSWDKLVFAGNTAGKSVQKI
ECCCHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCHHHHHHH
LAEERLYVFSKQCYSYRALAALINDINIPNVSISEINIPETLVEICNNELGIGIIPESIA
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEECCCCCHHHHHHHHCCCCCCEECCHHHE
LNYHFLNYETLPTEYASLRKTLIYHADHTISNGEKWLIEKSKPAFKS
EEEEEECCCCCCHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCCC
>Mature Secondary Structure
MDIENMKAFNKVAELKSISAAANELHHLQSNMSNKIKNIEKQFQTQLFFRHSNGVEPTKE
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHH
GEKIYQQFKKMILLWEETIDIINNEEETISIGITQSSLPMEFNTIIKEFYQQFPNKKLSI
HHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCHHHHHHHHHHHHHCCCCEEEE
VSGSTSELIPKIANRELTIAYVAELEKENLFQDSQIISQTLSWDKLVFAGNTAGKSVQKI
ECCCHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCHHHHHHH
LAEERLYVFSKQCYSYRALAALINDINIPNVSISEINIPETLVEICNNELGIGIIPESIA
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEECCCCCHHHHHHHHCCCCCCEECCHHHE
LNYHFLNYETLPTEYASLRKTLIYHADHTISNGEKWLIEKSKPAFKS
EEEEEECCCCCCHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA