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The map label for this gene is lysS

Identifier: 226222857

GI number: 226222857

Start: 232132

End: 233628

Strand: Direct

Name: lysS

Synonym: Lm4b_00226

Alternate gene names: 226222857

Gene position: 232132-233628 (Clockwise)

Preceding gene: 226222856

Following gene: 226222858

Centisome position: 7.97

GC content: 39.14

Gene sequence:

>1497_bases
ATGAGTAACGAGAATCATGAAGAACTAAATGACCAACTCATCGTCCGTCGCGAAAAAGTGGACACATTACGCGAAGAAGG
CATAGATCCTTTTGGCGAAAAATTCATCCGTTCCATCAGCCCGGAAGAAATTGAAACAAAATTCGCTGATAAATCAAAAG
AAGAACTTGAAGAAGCTGCTATTGAAGTTTCTGTAGCAGGTCGTATTATGACGAAACGTGTGAAAGGTAAAGTAGGTTTC
ACACATATTCAAGACCGTTTCCATCAATTACAAATCTATATCCGTAAAGATGCAATTGGCGAAGATGCTTATGCAGTCTT
CAAATTAGCGGATCTAGGTGACATTATTGGTATAAAAGGGACTATTTTCCGTACTAATACAGGTGAACTTTCTGTTAAAG
CAACGGAATTCACTTTGCTTTCCAAATCATTACGTCCACTTCCTGATAAATATCATGGCTTAAAAGACGTAGAACAACGC
TATCGTCAACGCTATTTAGACTTAATTACAAACGAAGAAAGCCAAAATCGTTTTGTGATGCGCAGTAAAATCTTGAAATA
CACTCGTGATTACATGGATAATCAAGGATTCCTAGAAGTAGAAACTCCAGTGCTTCATACAATTGCTGGTGGTGCTGCTG
CAAAACCATTTATTACGCACCATAATGCACTTGATATGGAATTATATTTACGAATTGCTTTAGAACTACACTTAAAACGC
CTAATCGTTGGTGGGATGGATAAAGTATACGAAATCGGCCGCGTTTTCCGTAATGAAGGAACCTCCACGCGTCATAACCC
AGAATTTACAATGTTAGAATCCTATGCAGCATACGAAGATTACGAAGATGTTATGGATTTGGTAGAAGGTTTAGTATCTA
CAGTATGCAAACAAGTAAATGGAACAACAGAAATAACATACGGTGAATACAATGTAGATCTAACTCCAAACTGGCGCCGT
ATCCACATGGCAGATGCTGTAAAAGAATATGTCGGAGTGGATTTCTGGAACGTAACTTCCGATGAAGAAGCACGCGAACT
AGCTAAAAAACATAATGTGCCAGTAACGGAGCACATGACATACGGGCACATTCTTAATGAGTTTTTTGAAACATACGTAG
AAGAAAAGCTTATTCAACCAACCTTTGTTTACGGACATCCGGTGGAAATTTCACCATTAGCGAAGAAAAACAAAGAAGAT
GACCGTTTTACTGACCGTTTTGAATTATTTATCGTTGGTCGTGAACATGCAAATGCATTCTCGGAATTAAACGATCCAAT
TGATCAAAGAGAGCGTTTTGAAGCGCAAATGAAAGAACGTGAGCAAGGTAATGATGAAGCGCACGGAATGGATGCAGATT
TCCTTGAAGCTTTAGAATACGGGCTACCGCCAACTGGTGGACTTGGAATAGGTATCGATCGTCTAGTAATGCTATTAACA
GATGCCCCGTCTATTCGTGATATCCTATTATTCCCAACTATGAAACACCGCGACTAA

Upstream 100 bases:

>100_bases
AGATTTCGGGGCAATTTTTAAATTTATACTAGAAAAACCAGACCAAAAAAGCGTATAATGGAAACATCTGTAGAAATGAA
TAGAATAGGAGTGTACCTAT

Downstream 100 bases:

>100_bases
TTAAAAATCCAGAGCTGATTATATCGGTTCTGGATTTTGTTTTTTTCTCAGAAAAAAACAAATTTGCCAAAGAGAAACTT
TTTCTCAAAATATGCTTGCA

Product: lysyl-tRNA synthetase

Products: NA

Alternate protein names: Lysine--tRNA ligase; LysRS [H]

Number of amino acids: Translated: 498; Mature: 497

Protein sequence:

>498_residues
MSNENHEELNDQLIVRREKVDTLREEGIDPFGEKFIRSISPEEIETKFADKSKEELEEAAIEVSVAGRIMTKRVKGKVGF
THIQDRFHQLQIYIRKDAIGEDAYAVFKLADLGDIIGIKGTIFRTNTGELSVKATEFTLLSKSLRPLPDKYHGLKDVEQR
YRQRYLDLITNEESQNRFVMRSKILKYTRDYMDNQGFLEVETPVLHTIAGGAAAKPFITHHNALDMELYLRIALELHLKR
LIVGGMDKVYEIGRVFRNEGTSTRHNPEFTMLESYAAYEDYEDVMDLVEGLVSTVCKQVNGTTEITYGEYNVDLTPNWRR
IHMADAVKEYVGVDFWNVTSDEEARELAKKHNVPVTEHMTYGHILNEFFETYVEEKLIQPTFVYGHPVEISPLAKKNKED
DRFTDRFELFIVGREHANAFSELNDPIDQRERFEAQMKEREQGNDEAHGMDADFLEALEYGLPPTGGLGIGIDRLVMLLT
DAPSIRDILLFPTMKHRD

Sequences:

>Translated_498_residues
MSNENHEELNDQLIVRREKVDTLREEGIDPFGEKFIRSISPEEIETKFADKSKEELEEAAIEVSVAGRIMTKRVKGKVGF
THIQDRFHQLQIYIRKDAIGEDAYAVFKLADLGDIIGIKGTIFRTNTGELSVKATEFTLLSKSLRPLPDKYHGLKDVEQR
YRQRYLDLITNEESQNRFVMRSKILKYTRDYMDNQGFLEVETPVLHTIAGGAAAKPFITHHNALDMELYLRIALELHLKR
LIVGGMDKVYEIGRVFRNEGTSTRHNPEFTMLESYAAYEDYEDVMDLVEGLVSTVCKQVNGTTEITYGEYNVDLTPNWRR
IHMADAVKEYVGVDFWNVTSDEEARELAKKHNVPVTEHMTYGHILNEFFETYVEEKLIQPTFVYGHPVEISPLAKKNKED
DRFTDRFELFIVGREHANAFSELNDPIDQRERFEAQMKEREQGNDEAHGMDADFLEALEYGLPPTGGLGIGIDRLVMLLT
DAPSIRDILLFPTMKHRD
>Mature_497_residues
SNENHEELNDQLIVRREKVDTLREEGIDPFGEKFIRSISPEEIETKFADKSKEELEEAAIEVSVAGRIMTKRVKGKVGFT
HIQDRFHQLQIYIRKDAIGEDAYAVFKLADLGDIIGIKGTIFRTNTGELSVKATEFTLLSKSLRPLPDKYHGLKDVEQRY
RQRYLDLITNEESQNRFVMRSKILKYTRDYMDNQGFLEVETPVLHTIAGGAAAKPFITHHNALDMELYLRIALELHLKRL
IVGGMDKVYEIGRVFRNEGTSTRHNPEFTMLESYAAYEDYEDVMDLVEGLVSTVCKQVNGTTEITYGEYNVDLTPNWRRI
HMADAVKEYVGVDFWNVTSDEEARELAKKHNVPVTEHMTYGHILNEFFETYVEEKLIQPTFVYGHPVEISPLAKKNKEDD
RFTDRFELFIVGREHANAFSELNDPIDQRERFEAQMKEREQGNDEAHGMDADFLEALEYGLPPTGGLGIGIDRLVMLLTD
APSIRDILLFPTMKHRD

Specific function: Also Can Synthesize A Number Of Adenyl Dinucleotides (In Particular Appppa). These Dinucleotides Have Been Proposed To Act As Modulators Of The Heat-Shock Response And Stress Response. [C]

COG id: COG1190

COG function: function code J; Lysyl-tRNA synthetase (class II)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI5031815, Length=503, Percent_Identity=43.7375745526839, Blast_Score=399, Evalue=1e-111,
Organism=Homo sapiens, GI194272210, Length=508, Percent_Identity=43.503937007874, Blast_Score=399, Evalue=1e-111,
Organism=Homo sapiens, GI40789249, Length=242, Percent_Identity=26.0330578512397, Blast_Score=81, Evalue=3e-15,
Organism=Homo sapiens, GI226491502, Length=466, Percent_Identity=21.6738197424893, Blast_Score=77, Evalue=3e-14,
Organism=Escherichia coli, GI1790571, Length=490, Percent_Identity=51.4285714285714, Blast_Score=513, Evalue=1e-146,
Organism=Escherichia coli, GI1789256, Length=490, Percent_Identity=51.4285714285714, Blast_Score=510, Evalue=1e-146,
Organism=Escherichia coli, GI87082379, Length=322, Percent_Identity=32.6086956521739, Blast_Score=149, Evalue=3e-37,
Organism=Escherichia coli, GI1788173, Length=333, Percent_Identity=26.1261261261261, Blast_Score=87, Evalue=3e-18,
Organism=Caenorhabditis elegans, GI17535927, Length=510, Percent_Identity=46.4705882352941, Blast_Score=427, Evalue=1e-120,
Organism=Caenorhabditis elegans, GI17535925, Length=510, Percent_Identity=46.4705882352941, Blast_Score=427, Evalue=1e-120,
Organism=Caenorhabditis elegans, GI71994340, Length=501, Percent_Identity=47.1057884231537, Blast_Score=427, Evalue=1e-120,
Organism=Caenorhabditis elegans, GI32566633, Length=172, Percent_Identity=26.1627906976744, Blast_Score=72, Evalue=5e-13,
Organism=Saccharomyces cerevisiae, GI6320242, Length=494, Percent_Identity=44.1295546558704, Blast_Score=393, Evalue=1e-110,
Organism=Saccharomyces cerevisiae, GI6324256, Length=474, Percent_Identity=33.1223628691983, Blast_Score=214, Evalue=2e-56,
Organism=Saccharomyces cerevisiae, GI6323011, Length=493, Percent_Identity=23.3265720081136, Blast_Score=92, Evalue=2e-19,
Organism=Saccharomyces cerevisiae, GI6321807, Length=476, Percent_Identity=24.7899159663866, Blast_Score=74, Evalue=6e-14,
Organism=Drosophila melanogaster, GI24640849, Length=506, Percent_Identity=43.0830039525692, Blast_Score=405, Evalue=1e-113,
Organism=Drosophila melanogaster, GI24640851, Length=506, Percent_Identity=43.0830039525692, Blast_Score=405, Evalue=1e-113,
Organism=Drosophila melanogaster, GI17136276, Length=476, Percent_Identity=24.3697478991597, Blast_Score=92, Evalue=8e-19,
Organism=Drosophila melanogaster, GI24584738, Length=260, Percent_Identity=26.9230769230769, Blast_Score=85, Evalue=1e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004364
- InterPro:   IPR018150
- InterPro:   IPR006195
- InterPro:   IPR002313
- InterPro:   IPR018149
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR004365 [H]

Pfam domain/function: PF00152 tRNA-synt_2; PF01336 tRNA_anti [H]

EC number: =6.1.1.6 [H]

Molecular weight: Translated: 57410; Mature: 57278

Theoretical pI: Translated: 4.92; Mature: 4.92

Prosite motif: PS00178 AA_TRNA_LIGASE_I ; PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNENHEELNDQLIVRREKVDTLREEGIDPFGEKFIRSISPEEIETKFADKSKEELEEAA
CCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCHHHHHHHHHCCCHHHHHHHH
IEVSVAGRIMTKRVKGKVGFTHIQDRFHQLQIYIRKDAIGEDAYAVFKLADLGDIIGIKG
HHHHHHHHHHHHHHCCCCCHHHHHHHHHHHEEEEECCCCCCCHHHHEEHHCCCCCCCCCE
TIFRTNTGELSVKATEFTLLSKSLRPLPDKYHGLKDVEQRYRQRYLDLITNEESQNRFVM
EEEECCCCCEEEEEHHHHHHHHHCCCCCHHHCCHHHHHHHHHHHHHHHHCCCCCCCHHHH
RSKILKYTRDYMDNQGFLEVETPVLHTIAGGAAAKPFITHHNALDMELYLRIALELHLKR
HHHHHHHHHHHHCCCCEEEECCCHHHHHCCCCCCCCEEECCCCCCHHHHHHHHHHHHHHH
LIVGGMDKVYEIGRVFRNEGTSTRHNPEFTMLESYAAYEDYEDVMDLVEGLVSTVCKQVN
HHHCCHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCC
GTTEITYGEYNVDLTPNWRRIHMADAVKEYVGVDFWNVTSDEEARELAKKHNVPVTEHMT
CCEEEEECEEEEEECCCCCEEHHHHHHHHHHCCCEECCCCCHHHHHHHHHCCCCCHHHHH
YGHILNEFFETYVEEKLIQPTFVYGHPVEISPLAKKNKEDDRFTDRFELFIVGREHANAF
HHHHHHHHHHHHHHHHHCCCEEEECCCCEECCHHHCCCCCCHHHCEEEEEEEECHHHHHH
SELNDPIDQRERFEAQMKEREQGNDEAHGMDADFLEALEYGLPPTGGLGIGIDRLVMLLT
HHHCCCHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCHHHHHHHHC
DAPSIRDILLFPTMKHRD
CCCCCCCEEECCCCCCCC
>Mature Secondary Structure 
SNENHEELNDQLIVRREKVDTLREEGIDPFGEKFIRSISPEEIETKFADKSKEELEEAA
CCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCHHHHHHHHHCCCHHHHHHHH
IEVSVAGRIMTKRVKGKVGFTHIQDRFHQLQIYIRKDAIGEDAYAVFKLADLGDIIGIKG
HHHHHHHHHHHHHHCCCCCHHHHHHHHHHHEEEEECCCCCCCHHHHEEHHCCCCCCCCCE
TIFRTNTGELSVKATEFTLLSKSLRPLPDKYHGLKDVEQRYRQRYLDLITNEESQNRFVM
EEEECCCCCEEEEEHHHHHHHHHCCCCCHHHCCHHHHHHHHHHHHHHHHCCCCCCCHHHH
RSKILKYTRDYMDNQGFLEVETPVLHTIAGGAAAKPFITHHNALDMELYLRIALELHLKR
HHHHHHHHHHHHCCCCEEEECCCHHHHHCCCCCCCCEEECCCCCCHHHHHHHHHHHHHHH
LIVGGMDKVYEIGRVFRNEGTSTRHNPEFTMLESYAAYEDYEDVMDLVEGLVSTVCKQVN
HHHCCHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCC
GTTEITYGEYNVDLTPNWRRIHMADAVKEYVGVDFWNVTSDEEARELAKKHNVPVTEHMT
CCEEEEECEEEEEECCCCCEEHHHHHHHHHHCCCEECCCCCHHHHHHHHHCCCCCHHHHH
YGHILNEFFETYVEEKLIQPTFVYGHPVEISPLAKKNKEDDRFTDRFELFIVGREHANAF
HHHHHHHHHHHHHHHHHCCCEEEECCCCEECCHHHCCCCCCHHHCEEEEEEEECHHHHHH
SELNDPIDQRERFEAQMKEREQGNDEAHGMDADFLEALEYGLPPTGGLGIGIDRLVMLLT
HHHCCCHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCHHHHHHHHC
DAPSIRDILLFPTMKHRD
CCCCCCCEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11679669 [H]