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The map label for this gene is dus1 [H]

Identifier: 226222856

GI number: 226222856

Start: 231022

End: 232017

Strand: Direct

Name: dus1 [H]

Synonym: Lm4b_00225

Alternate gene names: 226222856

Gene position: 231022-232017 (Clockwise)

Preceding gene: 226222855

Following gene: 226222857

Centisome position: 7.93

GC content: 42.47

Gene sequence:

>996_bases
ATGTTTAAAATAGGTAACGTAGAAATTAAAAACCAAGTAGTTGTGGCGCCAATGGCCGGTATATCCAATTCCGCATTCCG
TTTAACGGTCAAAGAATTCGGAGCAGGTCTCGTTTGCTGTGAAATGATTAGCGACAAAGGAATCGCATACCGCAATGCTA
AAACGCTTGATATGCTATATATTGATGAAAAAGAAAAACCATTGAGCTTACAAATTTTTGGTGGTGAGAAAGAAACGCTT
GTAGAAGCTGCGAAATTCGTAGCTGAAAATACAACGGCAGATATTATTGATATTAATATGGGCTGTCCGGTAAACAAAAT
TATCAAATGTGAAGCTGGAGCGAAATGGCTACTTGATCCAAATAAAGTGTATGACATGGTAGCTGCTGTAGTGGACGCTG
TTGATAAACCGGTAACAGTAAAAATGCGTATTGGTTGGGACGAAGAACATGTCTTTGCTATCGAAAATGCACTTGCTGCT
GAACGTGCCGGAGCTGCAGCTGTTGCAATGCACGGCCGTACTCGTGTGCAAATGTACGAAGGTAGCGCGAACTGGGATGT
GCTTAGAGACGTAAAACGCGAACTGAAAATCCCGTTCATGGCAAATGGGGACGTGAGAACACCAGAAGATGCCAAACGAA
TTTTAGAACATACTGGAGCAGATGGAGTAATGATTGGTCGAGCTGCACTTGGAAACCCATGGATGATTTATCGCACGGTG
AAATTCCTAGAAACAGGCGAACTTCTTCCAGAACCAGAACCACGCGAAAAAATGCAAACTGCCATGCTTCACCTTAATCG
CCTAGTCGAGCTAAAAGGAGAAAACATTGCAGTTCGCGAGTTTAGACAACACGCAGCTTATTATCTAAAAGGAGCTCGCG
GAAGCACTCGCGCGAAAGTGGCTGCTAACCAAGCGACAAAACAATCAGAGATGGAAGCAATTTTAAATGAATTTGTTCTT
CAATATGAAGAAAAAGCATTAGCGAAACAAGACTAA

Upstream 100 bases:

>100_bases
AATAAAAAATCAAGTCAACTGGTAAAATTGGCACTTGCAGAAAAAACTGATTATGATAACATATCACTAGCGCAATTTAA
GAGGAAGGGGTTTACCATTA

Downstream 100 bases:

>100_bases
TTAGGATTGCCTCGAGATTTCGGGGCAATTTTTAAATTTATACTAGAAAAACCAGACCAAAAAAGCGTATAATGGAAACA
TCTGTAGAAATGAATAGAAT

Product: hypothetical protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 331; Mature: 331

Protein sequence:

>331_residues
MFKIGNVEIKNQVVVAPMAGISNSAFRLTVKEFGAGLVCCEMISDKGIAYRNAKTLDMLYIDEKEKPLSLQIFGGEKETL
VEAAKFVAENTTADIIDINMGCPVNKIIKCEAGAKWLLDPNKVYDMVAAVVDAVDKPVTVKMRIGWDEEHVFAIENALAA
ERAGAAAVAMHGRTRVQMYEGSANWDVLRDVKRELKIPFMANGDVRTPEDAKRILEHTGADGVMIGRAALGNPWMIYRTV
KFLETGELLPEPEPREKMQTAMLHLNRLVELKGENIAVREFRQHAAYYLKGARGSTRAKVAANQATKQSEMEAILNEFVL
QYEEKALAKQD

Sequences:

>Translated_331_residues
MFKIGNVEIKNQVVVAPMAGISNSAFRLTVKEFGAGLVCCEMISDKGIAYRNAKTLDMLYIDEKEKPLSLQIFGGEKETL
VEAAKFVAENTTADIIDINMGCPVNKIIKCEAGAKWLLDPNKVYDMVAAVVDAVDKPVTVKMRIGWDEEHVFAIENALAA
ERAGAAAVAMHGRTRVQMYEGSANWDVLRDVKRELKIPFMANGDVRTPEDAKRILEHTGADGVMIGRAALGNPWMIYRTV
KFLETGELLPEPEPREKMQTAMLHLNRLVELKGENIAVREFRQHAAYYLKGARGSTRAKVAANQATKQSEMEAILNEFVL
QYEEKALAKQD
>Mature_331_residues
MFKIGNVEIKNQVVVAPMAGISNSAFRLTVKEFGAGLVCCEMISDKGIAYRNAKTLDMLYIDEKEKPLSLQIFGGEKETL
VEAAKFVAENTTADIIDINMGCPVNKIIKCEAGAKWLLDPNKVYDMVAAVVDAVDKPVTVKMRIGWDEEHVFAIENALAA
ERAGAAAVAMHGRTRVQMYEGSANWDVLRDVKRELKIPFMANGDVRTPEDAKRILEHTGADGVMIGRAALGNPWMIYRTV
KFLETGELLPEPEPREKMQTAMLHLNRLVELKGENIAVREFRQHAAYYLKGARGSTRAKVAANQATKQSEMEAILNEFVL
QYEEKALAKQD

Specific function: Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs [H]

COG id: COG0042

COG function: function code J; tRNA-dihydrouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dus family [H]

Homologues:

Organism=Homo sapiens, GI239788483, Length=231, Percent_Identity=29.8701298701299, Blast_Score=123, Evalue=3e-28,
Organism=Homo sapiens, GI239788462, Length=231, Percent_Identity=29.8701298701299, Blast_Score=122, Evalue=6e-28,
Organism=Homo sapiens, GI8923374, Length=247, Percent_Identity=29.5546558704453, Blast_Score=106, Evalue=3e-23,
Organism=Homo sapiens, GI31742496, Length=230, Percent_Identity=32.1739130434783, Blast_Score=103, Evalue=2e-22,
Organism=Homo sapiens, GI40807366, Length=224, Percent_Identity=29.9107142857143, Blast_Score=96, Evalue=4e-20,
Organism=Escherichia coli, GI1789660, Length=291, Percent_Identity=40.2061855670103, Blast_Score=214, Evalue=6e-57,
Organism=Escherichia coli, GI1788462, Length=298, Percent_Identity=29.1946308724832, Blast_Score=105, Evalue=3e-24,
Organism=Escherichia coli, GI145693211, Length=244, Percent_Identity=28.6885245901639, Blast_Score=81, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI17543114, Length=228, Percent_Identity=31.5789473684211, Blast_Score=117, Evalue=9e-27,
Organism=Caenorhabditis elegans, GI17507177, Length=233, Percent_Identity=31.3304721030043, Blast_Score=105, Evalue=3e-23,
Organism=Caenorhabditis elegans, GI17510279, Length=247, Percent_Identity=27.9352226720648, Blast_Score=92, Evalue=4e-19,
Organism=Caenorhabditis elegans, GI25144369, Length=222, Percent_Identity=30.6306306306306, Blast_Score=90, Evalue=2e-18,
Organism=Saccharomyces cerevisiae, GI6323560, Length=230, Percent_Identity=33.0434782608696, Blast_Score=107, Evalue=2e-24,
Organism=Saccharomyces cerevisiae, GI6323437, Length=223, Percent_Identity=27.3542600896861, Blast_Score=84, Evalue=4e-17,
Organism=Saccharomyces cerevisiae, GI6323433, Length=258, Percent_Identity=26.3565891472868, Blast_Score=83, Evalue=7e-17,
Organism=Drosophila melanogaster, GI24585320, Length=240, Percent_Identity=32.0833333333333, Blast_Score=119, Evalue=4e-27,
Organism=Drosophila melanogaster, GI24580595, Length=253, Percent_Identity=32.4110671936759, Blast_Score=113, Evalue=2e-25,
Organism=Drosophila melanogaster, GI19920448, Length=253, Percent_Identity=32.4110671936759, Blast_Score=113, Evalue=2e-25,
Organism=Drosophila melanogaster, GI45549423, Length=260, Percent_Identity=31.5384615384615, Blast_Score=104, Evalue=1e-22,
Organism=Drosophila melanogaster, GI19921524, Length=316, Percent_Identity=25.6329113924051, Blast_Score=88, Evalue=6e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR004652
- InterPro:   IPR001269
- InterPro:   IPR018517 [H]

Pfam domain/function: PF01207 Dus [H]

EC number: NA

Molecular weight: Translated: 36775; Mature: 36775

Theoretical pI: Translated: 6.39; Mature: 6.39

Prosite motif: PS01136 UPF0034

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
4.5 %Met     (Mature Protein)
5.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFKIGNVEIKNQVVVAPMAGISNSAFRLTVKEFGAGLVCCEMISDKGIAYRNAKTLDMLY
CCCCCCEEECCEEEEEECCCCCCCEEEEEHHHHCCCEEEEEHHHCCCCEEECCCEEEEEE
IDEKEKPLSLQIFGGEKETLVEAAKFVAENTTADIIDINMGCPVNKIIKCEAGAKWLLDP
ECCCCCCEEEEEECCCHHHHHHHHHHHHCCCCCEEEEEECCCCCCCEEEECCCCEEEECC
NKVYDMVAAVVDAVDKPVTVKMRIGWDEEHVFAIENALAAERAGAAAVAMHGRTRVQMYE
HHHHHHHHHHHHHCCCCEEEEEEECCCCCCEEHHHHHHHHHHCCCEEEEECCCEEEEEEE
GSANWDVLRDVKRELKIPFMANGDVRTPEDAKRILEHTGADGVMIGRAALGNPWMIYRTV
CCCCHHHHHHHHHHCCCCEEECCCCCCCHHHHHHHHHCCCCCEEEEHHHCCCCEEEEEEH
KFLETGELLPEPEPREKMQTAMLHLNRLVELKGENIAVREFRQHAAYYLKGARGSTRAKV
HHHHCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHEEECCCCCCHHHH
AANQATKQSEMEAILNEFVLQYEEKALAKQD
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MFKIGNVEIKNQVVVAPMAGISNSAFRLTVKEFGAGLVCCEMISDKGIAYRNAKTLDMLY
CCCCCCEEECCEEEEEECCCCCCCEEEEEHHHHCCCEEEEEHHHCCCCEEECCCEEEEEE
IDEKEKPLSLQIFGGEKETLVEAAKFVAENTTADIIDINMGCPVNKIIKCEAGAKWLLDP
ECCCCCCEEEEEECCCHHHHHHHHHHHHCCCCCEEEEEECCCCCCCEEEECCCCEEEECC
NKVYDMVAAVVDAVDKPVTVKMRIGWDEEHVFAIENALAAERAGAAAVAMHGRTRVQMYE
HHHHHHHHHHHHHCCCCEEEEEEECCCCCCEEHHHHHHHHHHCCCEEEEECCCEEEEEEE
GSANWDVLRDVKRELKIPFMANGDVRTPEDAKRILEHTGADGVMIGRAALGNPWMIYRTV
CCCCHHHHHHHHHHCCCCEEECCCCCCCHHHHHHHHHCCCCCEEEEHHHCCCCEEEEEEH
KFLETGELLPEPEPREKMQTAMLHLNRLVELKGENIAVREFRQHAAYYLKGARGSTRAKV
HHHHCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHEEECCCCCCHHHH
AANQATKQSEMEAILNEFVLQYEEKALAKQD
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7584024; 9384377 [H]