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The map label for this gene is folK

Identifier: 226222855

GI number: 226222855

Start: 230467

End: 230946

Strand: Direct

Name: folK

Synonym: Lm4b_00224

Alternate gene names: 226222855

Gene position: 230467-230946 (Clockwise)

Preceding gene: 226222854

Following gene: 226222856

Centisome position: 7.91

GC content: 36.46

Gene sequence:

>480_bases
ATGGCTAAGGTGTTTCTATCAATTGGTACAAACCTTGGCGAACGTTTAGAAAATTTAAATGACGCACTAAGTGGTTTAGC
TGCTTCAGATAAAATTAAAATTACCAACGTATCTAGTGTATACGAAACAGACGCAGTAGGTTACGAAGATCAAGCGGCAT
TTTTAAATATCGCTGTAGAAATTGAAACGGACTTTCCGCCAGTTGATTTACTAGATTTTTGTCTTGCGCTCGAACTTGAA
TTGGGGCGAGTTCGATTATTTAAGTGGGGTCCGCGACTCATTGATATCGATGTTTTATTATATGATGATGTTAAAATCGA
TACAGAAAAGCTGAAAATCCCACATCCTTATATGAAAGAACGCGCTTTTGTGCTGATTCCATTAATAGAGATTTCACCAG
AAAAAAGCAATCTTGTAGATAATCCAGCCATTTTGGAAGAACAAGGTGTTCGGAAAATAAAAAATCAAGTCAACTGGTAA

Upstream 100 bases:

>100_bases
TTAGAAGAAGTGACTGTAAAACTTATTAAACCAAATCCTCCGATTCCGGGACATTATGATTCTGTTGCAGTGGAAATTGA
ACGTAAAAGAAGTGATTTGA

Downstream 100 bases:

>100_bases
AATTGGCACTTGCAGAAAAAACTGATTATGATAACATATCACTAGCGCAATTTAAGAGGAAGGGGTTTACCATTAATGTT
TAAAATAGGTAACGTAGAAA

Product: 7,8-dihydro-6-hydroxymethylpterin pyrophosphokinase

Products: NA

Alternate protein names: 6-hydroxymethyl-7,8-dihydropterin pyrophosphokinase; PPPK; 7,8-dihydro-6-hydroxymethylpterin-pyrophosphokinase; HPPK [H]

Number of amino acids: Translated: 159; Mature: 158

Protein sequence:

>159_residues
MAKVFLSIGTNLGERLENLNDALSGLAASDKIKITNVSSVYETDAVGYEDQAAFLNIAVEIETDFPPVDLLDFCLALELE
LGRVRLFKWGPRLIDIDVLLYDDVKIDTEKLKIPHPYMKERAFVLIPLIEISPEKSNLVDNPAILEEQGVRKIKNQVNW

Sequences:

>Translated_159_residues
MAKVFLSIGTNLGERLENLNDALSGLAASDKIKITNVSSVYETDAVGYEDQAAFLNIAVEIETDFPPVDLLDFCLALELE
LGRVRLFKWGPRLIDIDVLLYDDVKIDTEKLKIPHPYMKERAFVLIPLIEISPEKSNLVDNPAILEEQGVRKIKNQVNW
>Mature_158_residues
AKVFLSIGTNLGERLENLNDALSGLAASDKIKITNVSSVYETDAVGYEDQAAFLNIAVEIETDFPPVDLLDFCLALELEL
GRVRLFKWGPRLIDIDVLLYDDVKIDTEKLKIPHPYMKERAFVLIPLIEISPEKSNLVDNPAILEEQGVRKIKNQVNW

Specific function: Dihydrofolate biosynthesis; first step. [C]

COG id: COG0801

COG function: function code H; 7,8-dihydro-6-hydroxymethylpterin-pyrophosphokinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HPPK family [H]

Homologues:

Organism=Escherichia coli, GI1786335, Length=135, Percent_Identity=43.7037037037037, Blast_Score=116, Evalue=6e-28,
Organism=Saccharomyces cerevisiae, GI6324073, Length=138, Percent_Identity=40.5797101449275, Blast_Score=110, Evalue=1e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000550 [H]

Pfam domain/function: PF01288 HPPK [H]

EC number: =2.7.6.3 [H]

Molecular weight: Translated: 17927; Mature: 17796

Theoretical pI: Translated: 4.32; Mature: 4.32

Prosite motif: PS00794 HPPK

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKVFLSIGTNLGERLENLNDALSGLAASDKIKITNVSSVYETDAVGYEDQAAFLNIAVE
CEEEEEEECCCHHHHHHHHHHHHHCCCCCCEEEEEECCHHHHCCCCCCCCCEEEEEEEEE
IETDFPPVDLLDFCLALELELGRVRLFKWGPRLIDIDVLLYDDVKIDTEKLKIPHPYMKE
EECCCCCHHHHHHHHHHHHCCCEEEEEECCCEEEEEEEEEECCCEECHHEEECCCCHHHC
RAFVLIPLIEISPEKSNLVDNPAILEEQGVRKIKNQVNW
CEEEEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
AKVFLSIGTNLGERLENLNDALSGLAASDKIKITNVSSVYETDAVGYEDQAAFLNIAVE
EEEEEEECCCHHHHHHHHHHHHHCCCCCCEEEEEECCHHHHCCCCCCCCCEEEEEEEEE
IETDFPPVDLLDFCLALELELGRVRLFKWGPRLIDIDVLLYDDVKIDTEKLKIPHPYMKE
EECCCCCHHHHHHHHHHHHCCCEEEEEECCCEEEEEEEEEECCCEECHHEEECCCCHHHC
RAFVLIPLIEISPEKSNLVDNPAILEEQGVRKIKNQVNW
CEEEEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7584024; 9384377; 2123867 [H]