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The map label for this gene is tilS/hprT [H]

Identifier: 226222848

GI number: 226222848

Start: 221151

End: 223097

Strand: Direct

Name: tilS/hprT [H]

Synonym: Lm4b_00217

Alternate gene names: 226222848

Gene position: 221151-223097 (Clockwise)

Preceding gene: 226222847

Following gene: 226222849

Centisome position: 7.59

GC content: 38.57

Gene sequence:

>1947_bases
ATGGATGACACCGATAAACGAGTACATAAGTATATCGAGAAACATGATTTAATCCGATCCGATGATAAATTGCTTGTGGC
AGTTTCTGGCGGTCCGGATTCTTTGGCGTTACTGCATTTTTTATGGAATTCAAATTTAGTCCCAAAAGAAGCAATTTCCG
TCGCACATATAAATCACCACTTAAGGGAAAATGCGGCGAATGAACAAAACGTCGTCGAAGCTTTTTGCGAGAGCCAGGGC
ATTCCTTTTTACATAGAAGAAGTAGACGTAAAAAGTCGAGCTGAGAGTTTGCAAAAAGGTATCGAGGAAACAGCGCGCAT
TGTCAGGTATGATTTCTTTGAGAAAGTAATGGCTGAGAAAAAGATCAACAAACTAGCGCTCGCACACCATGCAGATGACC
AAATCGAGACAATCTTAATGCGACTAGTTCGCGGAAGTGCTAGTATCGGCTGGTCAGGCATCCAACCAAAACGCGAGTTA
AAAGGAGGACAAGCAATCCGTCCTTTTTTACCAATTACAAAAGCAGAAATTATTGATTACGCCCAGAAACACGAATTGGC
ATACGAAATCGATGAATCAAATACTAGTCAAGAATACACAAGAAATCGTTACAGAGCGCAACTTTTACCGTTTTTAAAGC
AAGAAAATCCAGCTGTCTATTCGCACTTCGAAAGATTTTCAGAAGAAACAAGCGAAGACTTCCAATTTTTGGAGGCACTT
GCGAGTGATTTATTGAAGAAAAACCTCATCAAAAACGGCAAACGGACAACACTTTTACTTACTAACTTTAAAAATGAAGC
GAATCCTTTACAACGCCGCGCAATTCATTTACTATTGAGATATCTGTACAATGAAGATGCCAGTTTTATAACGGTAAATC
ACATATATCAGATTATCCAAATGATTCAAAGCGACAATCCATCTAGTTCGATTGACTTACCAAATAAACTCGTTGCAAAC
AGAGCATACGATAAACTTCATTTTCAATTTGGAGAAAGAGAAGCTCCTTCCGAGTTTTATCATCAACTAGAACTGAATGA
CCGCATTGAATTAGATAATAAAGCAAGTATCCGTTTAAAGTTAAAAAGTTCCGTTGTTCAAACAAATGGGCTAAATGGAA
TGCTACTGGATGCTGAAGAGATAACACTTCCTTTGATTGTTCGAAACCGTGTGAACGGCGACAGGATGACGATGAAAGGG
CAAGCAGGAAGTAAGAAACTTAAAGATATTTTCATCGATGCCAAAATACCAAGGCAAGAACGCGACAAGCTCCCTGTGAT
AACAGACTATACTGGGAAAATCCTTTGGGTTCCCGGTGTGAAGAAGTCTGCATATGACCGAGAATTTAGTCGTAGCAAAA
AGCAATACATTATTAGGTACACTCGAAATATAGGAGGAAACGAGAGCATGCATAATGATATTCAGAAAGTGCTGATATCG
GAGGACGAATTACAAGAGAAAATTCGTGAACTAGGTCGTGAGTTAACAACAGAATATGAAGGACGAAACCCATTAGTAGT
TGGGGTGTTAAAAGGAGCAACTCCTTTTATGACTGATTTACTTAAAAGAGTAGACACATACTTAGAAATGGACTTCATGG
ACGTATCCAGTTATGGAAATGGTACTGTATCATCGGGTGAAGTGAAAATCATTAAAGACCTTAATGCGTCAGTAGAAGGT
CGCGATGTACTTGTAATTGAAGATATCATTGATAGCGGACGTACACTTAGTTATCTAGTAGACCTAATCAAATACCGTAA
AGCAAAATCAGTTAAGTTGGTTACTTTACTTGATAAACCAGCTGGACGAAATGTAGAAATTGAAGCGGATTATGTAGGCT
TTGTCGTACCAAATGAATTCGTCGTTGGATATGGTTTGGATTATGCAGAACGTTACCGCAATTTACCATATATTGGTATT
TTAAAACCAGAAATATACAGCGAGTGA

Upstream 100 bases:

>100_bases
AAAATTAATTGTAAAGTAGATGCACCGTATTGGATCATTAATACGGTGCACTTTTTAATAAAAAATGTGAGAGCCGAATT
TCGAAGGAAGGTTTTCTTGA

Downstream 100 bases:

>100_bases
AATTAATTAACAAACAGAACAATATTTTGTTAAAATGTAATAGATAGCTTTTCAGAAAACGAAAACAAAAAATTGATGTC
AAACATGCAAAATAGTGGGC

Product: hypothetical protein

Products: NA

Alternate protein names: tRNA(Ile)-lysidine synthase; tRNA(Ile)-2-lysyl-cytidine synthase; tRNA(Ile)-lysidine synthetase; Hypoxanthine-guanine phosphoribosyltransferase; HGPRT; HGPRTase [H]

Number of amino acids: Translated: 648; Mature: 648

Protein sequence:

>648_residues
MDDTDKRVHKYIEKHDLIRSDDKLLVAVSGGPDSLALLHFLWNSNLVPKEAISVAHINHHLRENAANEQNVVEAFCESQG
IPFYIEEVDVKSRAESLQKGIEETARIVRYDFFEKVMAEKKINKLALAHHADDQIETILMRLVRGSASIGWSGIQPKREL
KGGQAIRPFLPITKAEIIDYAQKHELAYEIDESNTSQEYTRNRYRAQLLPFLKQENPAVYSHFERFSEETSEDFQFLEAL
ASDLLKKNLIKNGKRTTLLLTNFKNEANPLQRRAIHLLLRYLYNEDASFITVNHIYQIIQMIQSDNPSSSIDLPNKLVAN
RAYDKLHFQFGEREAPSEFYHQLELNDRIELDNKASIRLKLKSSVVQTNGLNGMLLDAEEITLPLIVRNRVNGDRMTMKG
QAGSKKLKDIFIDAKIPRQERDKLPVITDYTGKILWVPGVKKSAYDREFSRSKKQYIIRYTRNIGGNESMHNDIQKVLIS
EDELQEKIRELGRELTTEYEGRNPLVVGVLKGATPFMTDLLKRVDTYLEMDFMDVSSYGNGTVSSGEVKIIKDLNASVEG
RDVLVIEDIIDSGRTLSYLVDLIKYRKAKSVKLVTLLDKPAGRNVEIEADYVGFVVPNEFVVGYGLDYAERYRNLPYIGI
LKPEIYSE

Sequences:

>Translated_648_residues
MDDTDKRVHKYIEKHDLIRSDDKLLVAVSGGPDSLALLHFLWNSNLVPKEAISVAHINHHLRENAANEQNVVEAFCESQG
IPFYIEEVDVKSRAESLQKGIEETARIVRYDFFEKVMAEKKINKLALAHHADDQIETILMRLVRGSASIGWSGIQPKREL
KGGQAIRPFLPITKAEIIDYAQKHELAYEIDESNTSQEYTRNRYRAQLLPFLKQENPAVYSHFERFSEETSEDFQFLEAL
ASDLLKKNLIKNGKRTTLLLTNFKNEANPLQRRAIHLLLRYLYNEDASFITVNHIYQIIQMIQSDNPSSSIDLPNKLVAN
RAYDKLHFQFGEREAPSEFYHQLELNDRIELDNKASIRLKLKSSVVQTNGLNGMLLDAEEITLPLIVRNRVNGDRMTMKG
QAGSKKLKDIFIDAKIPRQERDKLPVITDYTGKILWVPGVKKSAYDREFSRSKKQYIIRYTRNIGGNESMHNDIQKVLIS
EDELQEKIRELGRELTTEYEGRNPLVVGVLKGATPFMTDLLKRVDTYLEMDFMDVSSYGNGTVSSGEVKIIKDLNASVEG
RDVLVIEDIIDSGRTLSYLVDLIKYRKAKSVKLVTLLDKPAGRNVEIEADYVGFVVPNEFVVGYGLDYAERYRNLPYIGI
LKPEIYSE
>Mature_648_residues
MDDTDKRVHKYIEKHDLIRSDDKLLVAVSGGPDSLALLHFLWNSNLVPKEAISVAHINHHLRENAANEQNVVEAFCESQG
IPFYIEEVDVKSRAESLQKGIEETARIVRYDFFEKVMAEKKINKLALAHHADDQIETILMRLVRGSASIGWSGIQPKREL
KGGQAIRPFLPITKAEIIDYAQKHELAYEIDESNTSQEYTRNRYRAQLLPFLKQENPAVYSHFERFSEETSEDFQFLEAL
ASDLLKKNLIKNGKRTTLLLTNFKNEANPLQRRAIHLLLRYLYNEDASFITVNHIYQIIQMIQSDNPSSSIDLPNKLVAN
RAYDKLHFQFGEREAPSEFYHQLELNDRIELDNKASIRLKLKSSVVQTNGLNGMLLDAEEITLPLIVRNRVNGDRMTMKG
QAGSKKLKDIFIDAKIPRQERDKLPVITDYTGKILWVPGVKKSAYDREFSRSKKQYIIRYTRNIGGNESMHNDIQKVLIS
EDELQEKIRELGRELTTEYEGRNPLVVGVLKGATPFMTDLLKRVDTYLEMDFMDVSSYGNGTVSSGEVKIIKDLNASVEG
RDVLVIEDIIDSGRTLSYLVDLIKYRKAKSVKLVTLLDKPAGRNVEIEADYVGFVVPNEFVVGYGLDYAERYRNLPYIGI
LKPEIYSE

Specific function: Ligates lysine onto the cytidine present at position 34 of the AUA codon-specific tRNA(Ile) that contains the anticodon CAU, in an ATP-dependent manner. Cytidine is converted to lysidine, thus changing the amino acid specificity of the tRNA from methionin

COG id: COG0037

COG function: function code D; Predicted ATPase of the PP-loop superfamily implicated in cell cycle control

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the purine/pyrimidine phosphoribosyltransferase family [H]

Homologues:

Organism=Homo sapiens, GI4504483, Length=179, Percent_Identity=34.0782122905028, Blast_Score=111, Evalue=2e-24,
Organism=Homo sapiens, GI9910262, Length=181, Percent_Identity=33.7016574585635, Blast_Score=99, Evalue=1e-20,
Organism=Escherichia coli, GI87081689, Length=166, Percent_Identity=49.3975903614458, Blast_Score=176, Evalue=4e-45,
Organism=Escherichia coli, GI1786386, Length=206, Percent_Identity=35.9223300970874, Blast_Score=116, Evalue=5e-27,
Organism=Caenorhabditis elegans, GI17510817, Length=180, Percent_Identity=31.1111111111111, Blast_Score=99, Evalue=6e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005904
- InterPro:   IPR012094
- InterPro:   IPR012796
- InterPro:   IPR012795
- InterPro:   IPR020825
- InterPro:   IPR000836
- InterPro:   IPR014729
- InterPro:   IPR011063 [H]

Pfam domain/function: PF01171 ATP_bind_3; PF00156 Pribosyltran; PF11734 TilS_C [H]

EC number: =2.4.2.8 [H]

Molecular weight: Translated: 74237; Mature: 74237

Theoretical pI: Translated: 6.59; Mature: 6.59

Prosite motif: PS00103 PUR_PYR_PR_TRANSFER

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDDTDKRVHKYIEKHDLIRSDDKLLVAVSGGPDSLALLHFLWNSNLVPKEAISVAHINHH
CCCHHHHHHHHHHHHHCCCCCCCEEEEECCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHH
LRENAANEQNVVEAFCESQGIPFYIEEVDVKSRAESLQKGIEETARIVRYDFFEKVMAEK
HHHCCCCHHHHHHHHHHCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KINKLALAHHADDQIETILMRLVRGSASIGWSGIQPKRELKGGQAIRPFLPITKAEIIDY
HHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCCCCHHHCCCCCCCCCCCCCHHHHHHHH
AQKHELAYEIDESNTSQEYTRNRYRAQLLPFLKQENPAVYSHFERFSEETSEDFQFLEAL
HHHCCEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHH
ASDLLKKNLIKNGKRTTLLLTNFKNEANPLQRRAIHLLLRYLYNEDASFITVNHIYQIIQ
HHHHHHHHHHCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHCCCCCEEEHHHHHHHHH
MIQSDNPSSSIDLPNKLVANRAYDKLHFQFGEREAPSEFYHQLELNDRIELDNKASIRLK
HHHCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCCCEECCCCCEEEEE
LKSSVVQTNGLNGMLLDAEEITLPLIVRNRVNGDRMTMKGQAGSKKLKDIFIDAKIPRQE
EEHHHHHCCCCCEEEEEHHHCEEEEEEECCCCCCEEEEECCCCCHHHHHEEECCCCCHHH
RDKLPVITDYTGKILWVPGVKKSAYDREFSRSKKQYIIRYTRNIGGNESMHNDIQKVLIS
HCCCCEEECCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCC
EDELQEKIRELGRELTTEYEGRNPLVVGVLKGATPFMTDLLKRVDTYLEMDFMDVSSYGN
HHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHEECCCCCC
GTVSSGEVKIIKDLNASVEGRDVLVIEDIIDSGRTLSYLVDLIKYRKAKSVKLVTLLDKP
CCCCCCCEEEEEECCCCCCCCEEEEEHHHHCCCCHHHHHHHHHHHHCCCCEEEEEEECCC
AGRNVEIEADYVGFVVPNEFVVGYGLDYAERYRNLPYIGILKPEIYSE
CCCCEEEEECEEEEEECCCEEEECCCCHHHHHCCCCEEEEECCHHCCC
>Mature Secondary Structure
MDDTDKRVHKYIEKHDLIRSDDKLLVAVSGGPDSLALLHFLWNSNLVPKEAISVAHINHH
CCCHHHHHHHHHHHHHCCCCCCCEEEEECCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHH
LRENAANEQNVVEAFCESQGIPFYIEEVDVKSRAESLQKGIEETARIVRYDFFEKVMAEK
HHHCCCCHHHHHHHHHHCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KINKLALAHHADDQIETILMRLVRGSASIGWSGIQPKRELKGGQAIRPFLPITKAEIIDY
HHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCCCCHHHCCCCCCCCCCCCCHHHHHHHH
AQKHELAYEIDESNTSQEYTRNRYRAQLLPFLKQENPAVYSHFERFSEETSEDFQFLEAL
HHHCCEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHH
ASDLLKKNLIKNGKRTTLLLTNFKNEANPLQRRAIHLLLRYLYNEDASFITVNHIYQIIQ
HHHHHHHHHHCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHCCCCCEEEHHHHHHHHH
MIQSDNPSSSIDLPNKLVANRAYDKLHFQFGEREAPSEFYHQLELNDRIELDNKASIRLK
HHHCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCCCEECCCCCEEEEE
LKSSVVQTNGLNGMLLDAEEITLPLIVRNRVNGDRMTMKGQAGSKKLKDIFIDAKIPRQE
EEHHHHHCCCCCEEEEEHHHCEEEEEEECCCCCCEEEEECCCCCHHHHHEEECCCCCHHH
RDKLPVITDYTGKILWVPGVKKSAYDREFSRSKKQYIIRYTRNIGGNESMHNDIQKVLIS
HCCCCEEECCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCC
EDELQEKIRELGRELTTEYEGRNPLVVGVLKGATPFMTDLLKRVDTYLEMDFMDVSSYGN
HHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHEECCCCCC
GTVSSGEVKIIKDLNASVEGRDVLVIEDIIDSGRTLSYLVDLIKYRKAKSVKLVTLLDKP
CCCCCCCEEEEEECCCCCCCCEEEEEHHHHCCCCHHHHHHHHHHHHCCCCEEEEEEECCC
AGRNVEIEADYVGFVVPNEFVVGYGLDYAERYRNLPYIGILKPEIYSE
CCCCEEEEECEEEEEECCCEEEECCCCHHHHHCCCCEEEEECCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11679669 [H]