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The map label for this gene is gidB [H]

Identifier: 225860884

GI number: 225860884

Start: 887749

End: 888462

Strand: Reverse

Name: gidB [H]

Synonym: SPT_0942

Alternate gene names: 225860884

Gene position: 888462-887749 (Counterclockwise)

Preceding gene: 225860887

Following gene: 225860883

Centisome position: 42.06

GC content: 41.74

Gene sequence:

>714_bases
ATGAAACCAAAAACATTTTACAACTTGCTTGCCGAGCAGAATCTTCCACTTTCGGACCAGCAAAAAGAACAATTTGAACG
TTATTTTGAGCTCTTGGTCGAGTGGAATGAGAAGATTAATTTGACGGCGATTACGGACAAGGAAGAAGTTTATCTCAAAC
ATTTTTACGATTCGATTGCACCCATTCTTCAAGGTTTGATTCCCAATGAAACTATCAAACTTCTTGATATCGGGGCTGGG
GCAGGATTTCCGAGTCTACCAATGAAAATTCTCTATCCGGAGTTAGATGTGACCATTATTGATTCACTCAATAAGCGCAT
CAACTTCCTACAACTCTTGGCTCAAGAACTGGATTTGAACGGAGTTCATTTCTACCACGGACGTGCCGAAGATTTTGCCC
AAGACAAGAACTTCCGTGCTCAATATGATTTTGTAACAGCTCGTGCGGTTGCCCGTATGCAGGTCCTATCTGAATTGACT
ATTCCCTACCTTAAGGTTGGTGGCAAACTATTAGCACTCAAGGCTAGCAATGCGCCTGAGGAATTATTAGAAGCTAAGAA
TGCCCTCAATCTCCTTTTTAGTAAGGTCGAAGACAATCTCAGCTACGCCCTACCGAATAGAGATCCGCGCTATATCACAG
TGGTAGAAAAGAAAAAAGAAACACCAAATAAATATCCACGTAAGGCTGGTATGCCAAATAAACGCCCACTTTAA

Upstream 100 bases:

>100_bases
AAGTCATAATATAAGTCCTTTCTGCTTTTAGATTAGTACCAGTATATCAAATTTTCTATGATTGTGGTAAAATAAGATGA
TACTAAAGAAGGAAATAACT

Downstream 100 bases:

>100_bases
ATTTTTTAGTAAACAAAGGTTTACAAAATCAGCCTCGCTCTTTTATTTCTAGGCTCGGGAAAAAATGATTTACAAAATCA
TTTTTTTCTGCTATACTATC

Product: 16S rRNA methyltransferase GidB

Products: NA

Alternate protein names: 16S rRNA 7-methylguanosine methyltransferase; 16S rRNA m7G methyltransferase [H]

Number of amino acids: Translated: 237; Mature: 237

Protein sequence:

>237_residues
MKPKTFYNLLAEQNLPLSDQQKEQFERYFELLVEWNEKINLTAITDKEEVYLKHFYDSIAPILQGLIPNETIKLLDIGAG
AGFPSLPMKILYPELDVTIIDSLNKRINFLQLLAQELDLNGVHFYHGRAEDFAQDKNFRAQYDFVTARAVARMQVLSELT
IPYLKVGGKLLALKASNAPEELLEAKNALNLLFSKVEDNLSYALPNRDPRYITVVEKKKETPNKYPRKAGMPNKRPL

Sequences:

>Translated_237_residues
MKPKTFYNLLAEQNLPLSDQQKEQFERYFELLVEWNEKINLTAITDKEEVYLKHFYDSIAPILQGLIPNETIKLLDIGAG
AGFPSLPMKILYPELDVTIIDSLNKRINFLQLLAQELDLNGVHFYHGRAEDFAQDKNFRAQYDFVTARAVARMQVLSELT
IPYLKVGGKLLALKASNAPEELLEAKNALNLLFSKVEDNLSYALPNRDPRYITVVEKKKETPNKYPRKAGMPNKRPL
>Mature_237_residues
MKPKTFYNLLAEQNLPLSDQQKEQFERYFELLVEWNEKINLTAITDKEEVYLKHFYDSIAPILQGLIPNETIKLLDIGAG
AGFPSLPMKILYPELDVTIIDSLNKRINFLQLLAQELDLNGVHFYHGRAEDFAQDKNFRAQYDFVTARAVARMQVLSELT
IPYLKVGGKLLALKASNAPEELLEAKNALNLLFSKVEDNLSYALPNRDPRYITVVEKKKETPNKYPRKAGMPNKRPL

Specific function: Specifically methylates the N7 position of a guanosine in 16S rRNA [H]

COG id: COG0357

COG function: function code M; Predicted S-adenosylmethionine-dependent methyltransferase involved in bacterial cell division

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RNA methyltransferase rsmG family [H]

Homologues:

Organism=Escherichia coli, GI1790179, Length=176, Percent_Identity=33.5227272727273, Blast_Score=100, Evalue=7e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003682 [H]

Pfam domain/function: PF02527 GidB [H]

EC number: 2.1.-.-

Molecular weight: Translated: 27199; Mature: 27199

Theoretical pI: Translated: 7.71; Mature: 7.71

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKPKTFYNLLAEQNLPLSDQQKEQFERYFELLVEWNEKINLTAITDKEEVYLKHFYDSIA
CCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCEEEEEECCHHHHHHHHHHHHHH
PILQGLIPNETIKLLDIGAGAGFPSLPMKILYPELDVTIIDSLNKRINFLQLLAQELDLN
HHHHCCCCCCEEEEEEECCCCCCCCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCC
GVHFYHGRAEDFAQDKNFRAQYDFVTARAVARMQVLSELTIPYLKVGGKLLALKASNAPE
CEEEECCCHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHCCCEEEEEECCCCHH
ELLEAKNALNLLFSKVEDNLSYALPNRDPRYITVVEKKKETPNKYPRKAGMPNKRPL
HHHHHHHHHHHHHHHHHCCCEEECCCCCCCEEEEEECCCCCCCCCCHHCCCCCCCCC
>Mature Secondary Structure
MKPKTFYNLLAEQNLPLSDQQKEQFERYFELLVEWNEKINLTAITDKEEVYLKHFYDSIA
CCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCEEEEEECCHHHHHHHHHHHHHH
PILQGLIPNETIKLLDIGAGAGFPSLPMKILYPELDVTIIDSLNKRINFLQLLAQELDLN
HHHHCCCCCCEEEEEEECCCCCCCCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCC
GVHFYHGRAEDFAQDKNFRAQYDFVTARAVARMQVLSELTIPYLKVGGKLLALKASNAPE
CEEEECCCHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHCCCEEEEEECCCCHH
ELLEAKNALNLLFSKVEDNLSYALPNRDPRYITVVEKKKETPNKYPRKAGMPNKRPL
HHHHHHHHHHHHHHHHHCCCEEECCCCCCCEEEEEECCCCCCCCCCHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA