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The map label for this gene is pyrP [H]

Identifier: 225860883

GI number: 225860883

Start: 886212

End: 887495

Strand: Reverse

Name: pyrP [H]

Synonym: SPT_0941

Alternate gene names: 225860883

Gene position: 887495-886212 (Counterclockwise)

Preceding gene: 225860884

Following gene: 225860879

Centisome position: 42.02

GC content: 43.15

Gene sequence:

>1284_bases
ATGAAACAAGAATCAACTGTTGATTTGTTACTAGACGTTGACCAACGTCCTTCGGCTGGAAAAGGAATTCTCCTTAGTTT
CCAACACGTTTTCGCCATGTTTGGTGCGACCATCTTGGTACCATTGATTTTGGGAATGCCTGTATCTGTTGCCCTTTTTG
CTTCAGGTGTTGGAACACTCATCTACATGATTGCTACTGGTTTTAAAGTTCCAGTTTATCTAGGTTCTTCATTCGCCTTT
ATCACAGCTATGTCACTGGCTATGAAAGAAATGGGGGGGGATGTATCTGCTGCCCAAACAGGGGTTATCTTGACTGGTTT
GGTCTATGTCCTTGTTGCTACCAGCATCCGATTTGTAGGAACAAAATGGATTGATAAACTCTTGCCACCAATCATTATCG
GTCCTATGATCATCGTTATCGGTCTTGGACTTGCAGGTTCAGCTGTTACCAATGCAGGTCTTGTAGCAGACGGAAATTGG
AAAAATGCTCTGGTAGCCGTTGTTACTTTCCTAATTGCTGCCTTTATCAATACAAAAGGAAAAGGCTTCCTACGAATCAT
TCCATTCCTCTTTGCCATTATCGGTGGTTACCTCTTCGCACTAACTCTTGGTTTGGTTGACTTTACGCCTGTCCTAGAAG
CTAACTGGTTTGAAATTCCTGGTTTCTACTTGCCATTTAGCACAGGTGGTGCCTTTAAAGAGTACAATCTTTACTTTGGT
CCAGAAGCCATCGCTATCTTGCCAATCGCTATCGTAACAATTTCTGAACATATCGGAGACCATACTGTTTTGGGTCAAAT
CTGTGGCCGTCAATTCTTAAAAGAACCAGGTCTTCACCGTACTCTTATTGGTGACGGTATCGCAACTTCTGTTTCTGCCT
TCCTTGGTGGACCAGCCAATACAACTTACGGAGAAAATACAGGGGTTATCGGTATGACTCGTATCGCTTCTGTCTCAGTT
ATCCGTAACGCTGCCTTCATCGCGATTGCCCTCAGCTTCCTTGGTAAATTCACTGCCTTGATTTCAACTATTCCAAACGC
TGTACTTGGTGGTATGTCAATCCTTCTCTATGGGGTTATCGCCAGCAATGGTTTGAAAGTCTTGATTAAAGAACGTGTTG
ATTTCGCTCAAATGCGAAACCTCATCATCGCAAGTGCTATGTTGGTTCTTGGACTTGGAGGAGCTATCCTTAAACTTGGT
CCAGTTACACTTTCAGGTACTGCCCTTTCAGCCATGACAGGAATCATCTTGAACTTGATCTTGCCATACGAAAATAAAGA
CTAA

Upstream 100 bases:

>100_bases
TTAAAACTCTTTAAAATCTAAATTTTAAAGAAGTCTTACTCTGAGGGCCTATTGCTGTAAAATAATGGGCTCTTTTTTGA
TGCCCAAAAGTGAGGTTTAT

Downstream 100 bases:

>100_bases
GAGTCTAAATACACCTAATCCACTCAGACAGCTGAGTGGATTTTTCGTATACCATAATAAAAGTGTCTTAACAAAATTAT
TAAAATCAAAAAACGTATAA

Product: uracil permease (Uracil transporter)

Products: Proton [Cytoplasm]; uracil [Cytoplasm] [C]

Alternate protein names: Uracil transporter [H]

Number of amino acids: Translated: 427; Mature: 427

Protein sequence:

>427_residues
MKQESTVDLLLDVDQRPSAGKGILLSFQHVFAMFGATILVPLILGMPVSVALFASGVGTLIYMIATGFKVPVYLGSSFAF
ITAMSLAMKEMGGDVSAAQTGVILTGLVYVLVATSIRFVGTKWIDKLLPPIIIGPMIIVIGLGLAGSAVTNAGLVADGNW
KNALVAVVTFLIAAFINTKGKGFLRIIPFLFAIIGGYLFALTLGLVDFTPVLEANWFEIPGFYLPFSTGGAFKEYNLYFG
PEAIAILPIAIVTISEHIGDHTVLGQICGRQFLKEPGLHRTLIGDGIATSVSAFLGGPANTTYGENTGVIGMTRIASVSV
IRNAAFIAIALSFLGKFTALISTIPNAVLGGMSILLYGVIASNGLKVLIKERVDFAQMRNLIIASAMLVLGLGGAILKLG
PVTLSGTALSAMTGIILNLILPYENKD

Sequences:

>Translated_427_residues
MKQESTVDLLLDVDQRPSAGKGILLSFQHVFAMFGATILVPLILGMPVSVALFASGVGTLIYMIATGFKVPVYLGSSFAF
ITAMSLAMKEMGGDVSAAQTGVILTGLVYVLVATSIRFVGTKWIDKLLPPIIIGPMIIVIGLGLAGSAVTNAGLVADGNW
KNALVAVVTFLIAAFINTKGKGFLRIIPFLFAIIGGYLFALTLGLVDFTPVLEANWFEIPGFYLPFSTGGAFKEYNLYFG
PEAIAILPIAIVTISEHIGDHTVLGQICGRQFLKEPGLHRTLIGDGIATSVSAFLGGPANTTYGENTGVIGMTRIASVSV
IRNAAFIAIALSFLGKFTALISTIPNAVLGGMSILLYGVIASNGLKVLIKERVDFAQMRNLIIASAMLVLGLGGAILKLG
PVTLSGTALSAMTGIILNLILPYENKD
>Mature_427_residues
MKQESTVDLLLDVDQRPSAGKGILLSFQHVFAMFGATILVPLILGMPVSVALFASGVGTLIYMIATGFKVPVYLGSSFAF
ITAMSLAMKEMGGDVSAAQTGVILTGLVYVLVATSIRFVGTKWIDKLLPPIIIGPMIIVIGLGLAGSAVTNAGLVADGNW
KNALVAVVTFLIAAFINTKGKGFLRIIPFLFAIIGGYLFALTLGLVDFTPVLEANWFEIPGFYLPFSTGGAFKEYNLYFG
PEAIAILPIAIVTISEHIGDHTVLGQICGRQFLKEPGLHRTLIGDGIATSVSAFLGGPANTTYGENTGVIGMTRIASVSV
IRNAAFIAIALSFLGKFTALISTIPNAVLGGMSILLYGVIASNGLKVLIKERVDFAQMRNLIIASAMLVLGLGGAILKLG
PVTLSGTALSAMTGIILNLILPYENKD

Specific function: Transport of uracil in the cell [H]

COG id: COG2233

COG function: function code F; Xanthine/uracil permeases

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the xanthine/uracil permease family. Nucleobase:cation symporter-2 (NCS2) (TC 2.A.40) subfamily [H]

Homologues:

Organism=Homo sapiens, GI44680145, Length=467, Percent_Identity=25.0535331905782, Blast_Score=100, Evalue=2e-21,
Organism=Homo sapiens, GI44680143, Length=471, Percent_Identity=24.8407643312102, Blast_Score=99, Evalue=7e-21,
Organism=Homo sapiens, GI44680148, Length=463, Percent_Identity=24.1900647948164, Blast_Score=94, Evalue=3e-19,
Organism=Homo sapiens, GI40316845, Length=463, Percent_Identity=24.1900647948164, Blast_Score=94, Evalue=3e-19,
Organism=Escherichia coli, GI1788843, Length=413, Percent_Identity=41.8886198547215, Blast_Score=295, Evalue=4e-81,
Organism=Escherichia coli, GI87081818, Length=434, Percent_Identity=35.7142857142857, Blast_Score=237, Evalue=1e-63,
Organism=Escherichia coli, GI87082181, Length=452, Percent_Identity=26.5486725663717, Blast_Score=115, Evalue=4e-27,
Organism=Escherichia coli, GI1790087, Length=472, Percent_Identity=27.5423728813559, Blast_Score=114, Evalue=2e-26,
Organism=Escherichia coli, GI87082178, Length=472, Percent_Identity=25, Blast_Score=96, Evalue=4e-21,
Organism=Caenorhabditis elegans, GI17558856, Length=458, Percent_Identity=22.4890829694323, Blast_Score=80, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI17541904, Length=452, Percent_Identity=22.5663716814159, Blast_Score=70, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI71993493, Length=434, Percent_Identity=23.2718894009217, Blast_Score=65, Evalue=5e-11,
Organism=Caenorhabditis elegans, GI17542262, Length=436, Percent_Identity=22.4770642201835, Blast_Score=65, Evalue=6e-11,
Organism=Drosophila melanogaster, GI21356175, Length=448, Percent_Identity=23.4375, Blast_Score=82, Evalue=5e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006042
- InterPro:   IPR006043 [H]

Pfam domain/function: PF00860 Xan_ur_permease [H]

EC number: NA

Molecular weight: Translated: 44804; Mature: 44804

Theoretical pI: Translated: 9.16; Mature: 9.16

Prosite motif: PS01116 XANTH_URACIL_PERMASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKQESTVDLLLDVDQRPSAGKGILLSFQHVFAMFGATILVPLILGMPVSVALFASGVGTL
CCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHH
IYMIATGFKVPVYLGSSFAFITAMSLAMKEMGGDVSAAQTGVILTGLVYVLVATSIRFVG
HHHHHCCCEEEEEECCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
TKWIDKLLPPIIIGPMIIVIGLGLAGSAVTNAGLVADGNWKNALVAVVTFLIAAFINTKG
HHHHHHHCCHHHHHHHHHHHHCCCCCCHHCCCCEEECCCCHHHHHHHHHHHHHHHHCCCC
KGFLRIIPFLFAIIGGYLFALTLGLVDFTPVLEANWFEIPGFYLPFSTGGAFKEYNLYFG
CHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHCCCCEECCCEEEECCCCCCCEECEEEEC
PEAIAILPIAIVTISEHIGDHTVLGQICGRQFLKEPGLHRTLIGDGIATSVSAFLGGPAN
CCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCEEHHCCCHHHHHHHHHCCCCC
TTYGENTGVIGMTRIASVSVIRNAAFIAIALSFLGKFTALISTIPNAVLGGMSILLYGVI
CCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ASNGLKVLIKERVDFAQMRNLIIASAMLVLGLGGAILKLGPVTLSGTALSAMTGIILNLI
HCCCCEEHHHHHCCHHHHHHHHHHHHHHHHHCCCHHHEECCEEECCHHHHHHHHHHHHHH
LPYENKD
CCCCCCC
>Mature Secondary Structure
MKQESTVDLLLDVDQRPSAGKGILLSFQHVFAMFGATILVPLILGMPVSVALFASGVGTL
CCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHH
IYMIATGFKVPVYLGSSFAFITAMSLAMKEMGGDVSAAQTGVILTGLVYVLVATSIRFVG
HHHHHCCCEEEEEECCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
TKWIDKLLPPIIIGPMIIVIGLGLAGSAVTNAGLVADGNWKNALVAVVTFLIAAFINTKG
HHHHHHHCCHHHHHHHHHHHHCCCCCCHHCCCCEEECCCCHHHHHHHHHHHHHHHHCCCC
KGFLRIIPFLFAIIGGYLFALTLGLVDFTPVLEANWFEIPGFYLPFSTGGAFKEYNLYFG
CHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHCCCCEECCCEEEECCCCCCCEECEEEEC
PEAIAILPIAIVTISEHIGDHTVLGQICGRQFLKEPGLHRTLIGDGIATSVSAFLGGPAN
CCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCEEHHCCCHHHHHHHHHCCCCC
TTYGENTGVIGMTRIASVSVIRNAAFIAIALSFLGKFTALISTIPNAVLGGMSILLYGVI
CCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ASNGLKVLIKERVDFAQMRNLIIASAMLVLGLGGAILKLGPVTLSGTALSAMTGIILNLI
HCCCCEEHHHHHCCHHHHHHHHHHHHHHHHHCCCHHHEECCEEECCHHHHHHHHHHHHHH
LPYENKD
CCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; uracil [Periplasm] [C]

Specific reaction: Proton [Periplasm] + uracil [Periplasm] = Proton [Cytoplasm] + uracil [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8206848 [H]