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The map label for this gene is hisF

Identifier: 225853516

GI number: 225853516

Start: 2026109

End: 2026894

Strand: Direct

Name: hisF

Synonym: BMEA_A2147

Alternate gene names: 225853516

Gene position: 2026109-2026894 (Clockwise)

Preceding gene: 225853515

Following gene: 225853517

Centisome position: 95.31

GC content: 60.18

Gene sequence:

>786_bases
ATGACTTTGAAAGCAAGAGTGATTCCCTGTCTCGATGTGAAAGATGGCCGCGTCGTCAAAGGTGTGAACTTCGTTGATCT
GATCGATGCCGGTGATCCGGTGGAAGCCGCCCGCGCCTATGATGCGGCAGGCGCCGACGAATTGTGCTTTCTCGACATCA
CGGCGTCTTCGGACAATCGCGAGACAATTTTCGATGTGGTGGCGCGTACAGCCGAACAGTGCTTCATGCCCTTGACCGTT
GGTGGCGGGGTTCGCCAGGTGGCCGATATCCGCAAGCTTCTTTTGGCGGGAGCCGACAAGGTGTCGATCAATACCGCAGC
GGTGAAGAACCCGGAATTCGTGGCGGAAGCCGCCGACAAGTTTGGCAATCAATGCATTGTTGTCGCCATCGATGCGAAAA
AGGTTTCCGGCGCGGGTGAAAATGATCACTGGGAAATCTTCACCCATGGCGGGCGGCAGCCGACCGGCATCGATGCGGTG
GAATTTGCACAGAAGGTCGTGGACCTTGGCGCAGGCGAAATCCTGCTCACCTCCATGGACCGCGATGGCACCAAGGCGGG
GTACGACGTGGCGCTGACCCGCGCCGTGGCCGATAGCGTGCGCGCGCCGGTCATCGCATCGGGCGGCGTCGGCACTCTCG
ATCATCTGGTGGCGGGCATTCGCGATGGCCATGCGACGGCGGTTCTCGCCGCTTCGATTTTCCACTTCGGCACCTATACG
ATCGGCGAGGCCAAGCGCTATATGGCCGAAGCCGGCATTCCGATGCGGCTCGATCCGGTCCGTTGA

Upstream 100 bases:

>100_bases
CCCCTGAAATGCGCAAGCTTGAAGGGGCGATTTCCGGCCGTGCGCTTTACGATGGCCGCATCGACCCGGCGGAAGCATTA
TCAGTATTGAGGGCTGCGGC

Downstream 100 bases:

>100_bases
TAGCAAGGGTGCATCAGAGCGGTTCCGGTTAAAAGGAAATCGCTCTAACTATTTGTTTTGTCGCATTTTCCAACGCAAAA
CCGTTTCACACTTGTGCTGG

Product: imidazole glycerol phosphate synthase subunit HisF

Products: NA

Alternate protein names: IGP synthase cyclase subunit; IGP synthase subunit hisF; ImGP synthase subunit hisF; IGPS subunit hisF

Number of amino acids: Translated: 261; Mature: 260

Protein sequence:

>261_residues
MTLKARVIPCLDVKDGRVVKGVNFVDLIDAGDPVEAARAYDAAGADELCFLDITASSDNRETIFDVVARTAEQCFMPLTV
GGGVRQVADIRKLLLAGADKVSINTAAVKNPEFVAEAADKFGNQCIVVAIDAKKVSGAGENDHWEIFTHGGRQPTGIDAV
EFAQKVVDLGAGEILLTSMDRDGTKAGYDVALTRAVADSVRAPVIASGGVGTLDHLVAGIRDGHATAVLAASIFHFGTYT
IGEAKRYMAEAGIPMRLDPVR

Sequences:

>Translated_261_residues
MTLKARVIPCLDVKDGRVVKGVNFVDLIDAGDPVEAARAYDAAGADELCFLDITASSDNRETIFDVVARTAEQCFMPLTV
GGGVRQVADIRKLLLAGADKVSINTAAVKNPEFVAEAADKFGNQCIVVAIDAKKVSGAGENDHWEIFTHGGRQPTGIDAV
EFAQKVVDLGAGEILLTSMDRDGTKAGYDVALTRAVADSVRAPVIASGGVGTLDHLVAGIRDGHATAVLAASIFHFGTYT
IGEAKRYMAEAGIPMRLDPVR
>Mature_260_residues
TLKARVIPCLDVKDGRVVKGVNFVDLIDAGDPVEAARAYDAAGADELCFLDITASSDNRETIFDVVARTAEQCFMPLTVG
GGVRQVADIRKLLLAGADKVSINTAAVKNPEFVAEAADKFGNQCIVVAIDAKKVSGAGENDHWEIFTHGGRQPTGIDAVE
FAQKVVDLGAGEILLTSMDRDGTKAGYDVALTRAVADSVRAPVIASGGVGTLDHLVAGIRDGHATAVLAASIFHFGTYTI
GEAKRYMAEAGIPMRLDPVR

Specific function: IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The hisF subunit catalyzes the cyclization activity that produces IGP and AICAR from PRFAR using the ammonia provided by the hisH subunit

COG id: COG0107

COG function: function code E; Imidazoleglycerol-phosphate synthase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hisA/hisF family

Homologues:

Organism=Escherichia coli, GI1788336, Length=257, Percent_Identity=42.8015564202335, Blast_Score=199, Evalue=1e-52,
Organism=Escherichia coli, GI87082028, Length=244, Percent_Identity=29.9180327868852, Blast_Score=96, Evalue=2e-21,
Organism=Saccharomyces cerevisiae, GI6319725, Length=315, Percent_Identity=32.3809523809524, Blast_Score=147, Evalue=2e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HIS6_BRUMB (C0RFX3)

Other databases:

- EMBL:   CP001488
- RefSeq:   YP_002733749.1
- ProteinModelPortal:   C0RFX3
- SMR:   C0RFX3
- GeneID:   7678114
- GenomeReviews:   CP001488_GR
- KEGG:   bmi:BMEA_A2147
- OMA:   RVVKGTN
- ProtClustDB:   PRK02083
- GO:   GO:0005737
- HAMAP:   MF_01013
- InterPro:   IPR013785
- InterPro:   IPR006062
- InterPro:   IPR004651
- InterPro:   IPR011060
- Gene3D:   G3DSA:3.20.20.70
- TIGRFAMs:   TIGR00735

Pfam domain/function: PF00977 His_biosynth; SSF51366 RibP_bind_barrel

EC number: 4.1.3.-

Molecular weight: Translated: 27456; Mature: 27324

Theoretical pI: Translated: 4.87; Mature: 4.87

Prosite motif: NA

Important sites: ACT_SITE 12-12 ACT_SITE 131-131

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLKARVIPCLDVKDGRVVKGVNFVDLIDAGDPVEAARAYDAAGADELCFLDITASSDNR
CCCCCEEEEEEECCCCCEEECCCEEEEECCCCCHHHHHHHCCCCCCCEEEEEEECCCCCH
ETIFDVVARTAEQCFMPLTVGGGVRQVADIRKLLLAGADKVSINTAAVKNPEFVAEAADK
HHHHHHHHHHHHHHEEEEEECCCHHHHHHHHHHHHCCCCCEEEEHEECCCHHHHHHHHHH
FGNQCIVVAIDAKKVSGAGENDHWEIFTHGGRQPTGIDAVEFAQKVVDLGAGEILLTSMD
HCCEEEEEEEECEECCCCCCCCCEEEEECCCCCCCCCHHHHHHHHHHHCCCCEEEEEECC
RDGTKAGYDVALTRAVADSVRAPVIASGGVGTLDHLVAGIRDGHATAVLAASIFHFGTYT
CCCCCCCHHHHHHHHHHHHHCCCEEECCCCCHHHHHHHHCCCCCHHHHHHHHHHHHCCEE
IGEAKRYMAEAGIPMRLDPVR
HHHHHHHHHHCCCCEEECCCC
>Mature Secondary Structure 
TLKARVIPCLDVKDGRVVKGVNFVDLIDAGDPVEAARAYDAAGADELCFLDITASSDNR
CCCCEEEEEEECCCCCEEECCCEEEEECCCCCHHHHHHHCCCCCCCEEEEEEECCCCCH
ETIFDVVARTAEQCFMPLTVGGGVRQVADIRKLLLAGADKVSINTAAVKNPEFVAEAADK
HHHHHHHHHHHHHHEEEEEECCCHHHHHHHHHHHHCCCCCEEEEHEECCCHHHHHHHHHH
FGNQCIVVAIDAKKVSGAGENDHWEIFTHGGRQPTGIDAVEFAQKVVDLGAGEILLTSMD
HCCEEEEEEEECEECCCCCCCCCEEEEECCCCCCCCCHHHHHHHHHHHCCCCEEEEEECC
RDGTKAGYDVALTRAVADSVRAPVIASGGVGTLDHLVAGIRDGHATAVLAASIFHFGTYT
CCCCCCCHHHHHHHHHHHHHCCCEEECCCCCHHHHHHHHCCCCCHHHHHHHHHHHHCCEE
IGEAKRYMAEAGIPMRLDPVR
HHHHHHHHHHCCCCEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Lyases; Carbon-Nitrogen Lyases; Amidine-Lyases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA