Click here to switch to the map view.

The map label for this gene is cobJ [H]

Identifier: 225852781

GI number: 225852781

Start: 1269622

End: 1271367

Strand: Direct

Name: cobJ [H]

Synonym: BMEA_A1336

Alternate gene names: 225852781

Gene position: 1269622-1271367 (Clockwise)

Preceding gene: 225852780

Following gene: 225852784

Centisome position: 59.73

GC content: 60.48

Gene sequence:

>1746_bases
TTGCCCCTATTTCTCGCTCATTCTGGTGCCCGGCGAAAAATGGAACGGCGCATGATCCCCGCCATTCTCGTTCTTGCAGA
AGCAGCACTCCCGACCACGCGCCAGATCGCGGCCGCATTGGAACGCGCTGAAGTTCTGGGCCTGCACGACCGCGTGAGCA
CCGCCGACAAAACCTTCGCCCATTTCGGAGAGGCCCTTCGGGCGCTTTATGAGGAAGGGCGCCCCGTTATTGCGCTTTGC
GCGGCTGGCATCGTCATTCGTGCTCTTGCGCCACTTCTGCAAAACAAGCGCATGGAGCCGCCGGTGCTCGCAGTGGCAGA
AGATGGAAGCGCCGTCGTGCCGCTTCTGGGCGGGCTTTCCGGCGTCAACGATCTGGCGCGGCAGATCGCGGCCGCGCTCG
GCATTGCGCCCGCAATCACCACATCCGGTGAATTGCGCTTCGGCATCAATCTTCTGCATCCGCCTTCGGATCTGACGCTC
GCCAATCCCGATGATGCCAAAACCTTCACCTCCAATCTTCTGGCCGGTGAAAGACTGAAGCTCAACGGAAAATCCAATTG
GCTGCGGCAATCGAAACTTCCCTTTGCCGATGATGGCAAGCTTACCATCACCATCACGCCGCATAATCGTACACCGCGCA
CGAATGAGTTGATCTATCATCCGCAATGCGTGGCAATTGGCATCGAAACACCGGATGAGAGCCTACAGGGCCAGATAGAA
ACCGCATTGGCCGGGGCCGGGCTGGCAAAAACATCGCTCGCCCTCCTCCTCGCGCATGAGAAGGATTGCACCTCTCCCTT
TCTCCATGAAACAGCCCGTAGGCTCAATGTCCAGTTGCGCTTCGTGACCGGCGCTCCCGATGCGCGTGAACTTGCTTTGC
GCTCTGTCGAACATCCTTTGGACCTGATCGAAAGCAGCGGGCTTGCCCTCGCCGTGGCCAAAGCCCCGGCGGATGTTCTT
TTCGTCGGGAGAAAGCGCGGCAGGCTTGCCGTGGTCGGACTTGGTCCCGGCGCGCGTGACCTGATGACGCCTGCCGTGCA
GCGCGAACTGGAACAGGCGGAGGATATTCTGGGCTACGAAACCTATGTGCGCATGGCAGGCCCCTTCCGCACCGGGCAGA
CCATCCATATGACGGACAATCGCGAGGAAATGCAGCGCGCGCGCCATGCCTTCGCGCTCGCTGCATCGGGGCATAATGTG
GCGATGGTTTCCTCTGGCGATCCCGGTGTTTTCGCAATGGCGGCGGCAGTGGTCGAGGCCCTGCATGAATCGGATAACCC
GGCATGGCACGGCGTAGAGCTGTCCATTCAGCCCGGTATTTCCGCCGCCATGGCAGCAGCTTCGCGCATCGGCGCACCGC
TTGGCCATGATTTCTGCATCATTTCGCTGTCGGACAATCTGAAGCCGTGGGATATCATCGAAAAACGGCTGGCGCTTGCC
GCCGAAGCCGATCTCGCCATGGCCTTCTATAATCCGATCTCAAAAGCGCGCCCGCACCAGCTTGGCCGCGCGCTGGAACT
CCTGCGCCAATACCGCTCCGGCGAAACACCCGTCGTTCTGGGGCGCGACATCGGCAGAGCTGGAGAGACAACCCGCGTGA
TGACCCTCGGTGCACTGACGCCCGGCGATGTCGATATGCGCACGGTGGTTATCATCGGCTCATCGCACACGCGCCGCTTT
GCGCAGCCTGATGGTGGCGAATGGGTTTATACGCCGCGCTGGTATGGTCAAAAGCCGGATAAATAA

Upstream 100 bases:

>100_bases
TGCTGGGCGATCTCGGCCTCATGGGCCGCGCGCTTTATGTCGAGCGCGCCACCATGGCCAATCAACGTATCGTGCCGTTG
GCCGAGGTTAATGGCAGCGA

Downstream 100 bases:

>100_bases
AACAAAAAAGGCAGGAAAACCCTGCCTTTTTCGTAAGTTAACAACGATTGTTGAATCGCTTCTTTTTCTTTGCCGCGGCC
TTTTTCGGCTGGGCGGCTGC

Product: precorrin-3B C17-methyltransferase

Products: NA

Alternate protein names: Precorrin-3 methylase; Precorrin-3 methyltransferase [H]

Number of amino acids: Translated: 581; Mature: 580

Protein sequence:

>581_residues
MPLFLAHSGARRKMERRMIPAILVLAEAALPTTRQIAAALERAEVLGLHDRVSTADKTFAHFGEALRALYEEGRPVIALC
AAGIVIRALAPLLQNKRMEPPVLAVAEDGSAVVPLLGGLSGVNDLARQIAAALGIAPAITTSGELRFGINLLHPPSDLTL
ANPDDAKTFTSNLLAGERLKLNGKSNWLRQSKLPFADDGKLTITITPHNRTPRTNELIYHPQCVAIGIETPDESLQGQIE
TALAGAGLAKTSLALLLAHEKDCTSPFLHETARRLNVQLRFVTGAPDARELALRSVEHPLDLIESSGLALAVAKAPADVL
FVGRKRGRLAVVGLGPGARDLMTPAVQRELEQAEDILGYETYVRMAGPFRTGQTIHMTDNREEMQRARHAFALAASGHNV
AMVSSGDPGVFAMAAAVVEALHESDNPAWHGVELSIQPGISAAMAAASRIGAPLGHDFCIISLSDNLKPWDIIEKRLALA
AEADLAMAFYNPISKARPHQLGRALELLRQYRSGETPVVLGRDIGRAGETTRVMTLGALTPGDVDMRTVVIIGSSHTRRF
AQPDGGEWVYTPRWYGQKPDK

Sequences:

>Translated_581_residues
MPLFLAHSGARRKMERRMIPAILVLAEAALPTTRQIAAALERAEVLGLHDRVSTADKTFAHFGEALRALYEEGRPVIALC
AAGIVIRALAPLLQNKRMEPPVLAVAEDGSAVVPLLGGLSGVNDLARQIAAALGIAPAITTSGELRFGINLLHPPSDLTL
ANPDDAKTFTSNLLAGERLKLNGKSNWLRQSKLPFADDGKLTITITPHNRTPRTNELIYHPQCVAIGIETPDESLQGQIE
TALAGAGLAKTSLALLLAHEKDCTSPFLHETARRLNVQLRFVTGAPDARELALRSVEHPLDLIESSGLALAVAKAPADVL
FVGRKRGRLAVVGLGPGARDLMTPAVQRELEQAEDILGYETYVRMAGPFRTGQTIHMTDNREEMQRARHAFALAASGHNV
AMVSSGDPGVFAMAAAVVEALHESDNPAWHGVELSIQPGISAAMAAASRIGAPLGHDFCIISLSDNLKPWDIIEKRLALA
AEADLAMAFYNPISKARPHQLGRALELLRQYRSGETPVVLGRDIGRAGETTRVMTLGALTPGDVDMRTVVIIGSSHTRRF
AQPDGGEWVYTPRWYGQKPDK
>Mature_580_residues
PLFLAHSGARRKMERRMIPAILVLAEAALPTTRQIAAALERAEVLGLHDRVSTADKTFAHFGEALRALYEEGRPVIALCA
AGIVIRALAPLLQNKRMEPPVLAVAEDGSAVVPLLGGLSGVNDLARQIAAALGIAPAITTSGELRFGINLLHPPSDLTLA
NPDDAKTFTSNLLAGERLKLNGKSNWLRQSKLPFADDGKLTITITPHNRTPRTNELIYHPQCVAIGIETPDESLQGQIET
ALAGAGLAKTSLALLLAHEKDCTSPFLHETARRLNVQLRFVTGAPDARELALRSVEHPLDLIESSGLALAVAKAPADVLF
VGRKRGRLAVVGLGPGARDLMTPAVQRELEQAEDILGYETYVRMAGPFRTGQTIHMTDNREEMQRARHAFALAASGHNVA
MVSSGDPGVFAMAAAVVEALHESDNPAWHGVELSIQPGISAAMAAASRIGAPLGHDFCIISLSDNLKPWDIIEKRLALAA
EADLAMAFYNPISKARPHQLGRALELLRQYRSGETPVVLGRDIGRAGETTRVMTLGALTPGDVDMRTVVIIGSSHTRRFA
QPDGGEWVYTPRWYGQKPDK

Specific function: Methyltransferase that catalyzes the methylation of C-17 in precorrin-3B to form precorrin-4 [H]

COG id: COG2073

COG function: function code H; Cobalamin biosynthesis protein CbiG

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the precorrin methyltransferase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000878
- InterPro:   IPR014777
- InterPro:   IPR014776
- InterPro:   IPR006363 [H]

Pfam domain/function: PF00590 TP_methylase [H]

EC number: =2.1.1.131 [H]

Molecular weight: Translated: 62463; Mature: 62331

Theoretical pI: Translated: 7.52; Mature: 7.52

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPLFLAHSGARRKMERRMIPAILVLAEAALPTTRQIAAALERAEVLGLHDRVSTADKTFA
CCEEEECCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHH
HFGEALRALYEEGRPVIALCAAGIVIRALAPLLQNKRMEPPVLAVAEDGSAVVPLLGGLS
HHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCEEHHHHCCCC
GVNDLARQIAAALGIAPAITTSGELRFGINLLHPPSDLTLANPDDAKTFTSNLLAGERLK
CHHHHHHHHHHHHCCCHHCCCCCCEEEEEEEECCCCCCEECCCCCHHHHHHHHCCCCEEE
LNGKSNWLRQSKLPFADDGKLTITITPHNRTPRTNELIYHPQCVAIGIETPDESLQGQIE
ECCCCCHHHHCCCCCCCCCEEEEEEECCCCCCCCCCEEECCEEEEEEECCCCHHHHHHHH
TALAGAGLAKTSLALLLAHEKDCTSPFLHETARRLNVQLRFVTGAPDARELALRSVEHPL
HHHHCCCHHHHHHHHEEECCCCCCCHHHHHHHHHEEEEEEEEECCCCHHHHHHHHHHCHH
DLIESSGLALAVAKAPADVLFVGRKRGRLAVVGLGPGARDLMTPAVQRELEQAEDILGYE
HHHHCCCCEEEEECCCCEEEEEECCCCCEEEEECCCCCHHHHCHHHHHHHHHHHHHHCHH
TYVRMAGPFRTGQTIHMTDNREEMQRARHAFALAASGHNVAMVSSGDPGVFAMAAAVVEA
HHHEECCCCCCCCEEEECCCHHHHHHHHHHEEEEECCCCEEEEECCCCCHHHHHHHHHHH
LHESDNPAWHGVELSIQPGISAAMAAASRIGAPLGHDFCIISLSDNLKPWDIIEKRLALA
HHCCCCCCEEEEEEEECCCHHHHHHHHHHHCCCCCCCEEEEEECCCCCHHHHHHHHHHHH
AEADLAMAFYNPISKARPHQLGRALELLRQYRSGETPVVLGRDIGRAGETTRVMTLGALT
HHCHHHHHHHCCHHHCCHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCEEEEEEECCC
PGDVDMRTVVIIGSSHTRRFAQPDGGEWVYTPRWYGQKPDK
CCCCCEEEEEEEECCCCCCCCCCCCCCEEECCCCCCCCCCC
>Mature Secondary Structure 
PLFLAHSGARRKMERRMIPAILVLAEAALPTTRQIAAALERAEVLGLHDRVSTADKTFA
CEEEECCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHH
HFGEALRALYEEGRPVIALCAAGIVIRALAPLLQNKRMEPPVLAVAEDGSAVVPLLGGLS
HHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCEEHHHHCCCC
GVNDLARQIAAALGIAPAITTSGELRFGINLLHPPSDLTLANPDDAKTFTSNLLAGERLK
CHHHHHHHHHHHHCCCHHCCCCCCEEEEEEEECCCCCCEECCCCCHHHHHHHHCCCCEEE
LNGKSNWLRQSKLPFADDGKLTITITPHNRTPRTNELIYHPQCVAIGIETPDESLQGQIE
ECCCCCHHHHCCCCCCCCCEEEEEEECCCCCCCCCCEEECCEEEEEEECCCCHHHHHHHH
TALAGAGLAKTSLALLLAHEKDCTSPFLHETARRLNVQLRFVTGAPDARELALRSVEHPL
HHHHCCCHHHHHHHHEEECCCCCCCHHHHHHHHHEEEEEEEEECCCCHHHHHHHHHHCHH
DLIESSGLALAVAKAPADVLFVGRKRGRLAVVGLGPGARDLMTPAVQRELEQAEDILGYE
HHHHCCCCEEEEECCCCEEEEEECCCCCEEEEECCCCCHHHHCHHHHHHHHHHHHHHCHH
TYVRMAGPFRTGQTIHMTDNREEMQRARHAFALAASGHNVAMVSSGDPGVFAMAAAVVEA
HHHEECCCCCCCCEEEECCCHHHHHHHHHHEEEEECCCCEEEEECCCCCHHHHHHHHHHH
LHESDNPAWHGVELSIQPGISAAMAAASRIGAPLGHDFCIISLSDNLKPWDIIEKRLALA
HHCCCCCCEEEEEEEECCCHHHHHHHHHHHCCCCCCCEEEEEECCCCCHHHHHHHHHHHH
AEADLAMAFYNPISKARPHQLGRALELLRQYRSGETPVVLGRDIGRAGETTRVMTLGALT
HHCHHHHHHHCCHHHCCHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCEEEEEEECCC
PGDVDMRTVVIIGSSHTRRFAQPDGGEWVYTPRWYGQKPDK
CCCCCEEEEEEEECCCCCCCCCCCCCCEEECCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2211521; 8226690 [H]