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The map label for this gene is livJ [C]

Identifier: 225852784

GI number: 225852784

Start: 1273485

End: 1274672

Strand: Direct

Name: livJ [C]

Synonym: BMEA_A1340

Alternate gene names: 225852784

Gene position: 1273485-1274672 (Clockwise)

Preceding gene: 225852781

Following gene: 225852789

Centisome position: 59.91

GC content: 58.75

Gene sequence:

>1188_bases
TTGCCTGCATCCATTACGGGCATTGCACAAAGGCGAGGAAACCGCTACCCACTTTTCTATCAAATTGCCACGAATTGTTT
AAAGAAGACCGATCACGTGATTGCGCCAGGGAAATGTCCGGCTACCGTGCTGCTGACAAGCATTGTCCTCTCTTGGCTGG
CCAGTGGTGCGCTGGCACAGGCGGAAGATGTGCGCGTCGGGCTTGCGGCGCCGCTTTCCGGCACCTTCGCACCGCTGGGC
AACCAACTGGTTGAAGGCGCGCGCGCGGCAGCAGGAACGGGCGTCCAGCTGACCGTTTTTGATGATCGCTGCGACGCCGA
GGGCGGCAAGGAAGCTGCCGAACGTTTTGTTGAGCAGAATATACGCATCGCCACCGGCTTTCTATGCCCGGAAGCCTTGG
AGACCGCGCTTCCCATCCTTTCGCAACGCAATATCCCGATCATCATATCAGACGTCAGCGAACTGACCCTTGCCGAAAGA
CGCGCCGCCTCGCCGCTTCCCACCTTTCGCCTGACGACCGGGCTCAATAAGGAAACGCAGGCAACGGGCAGCTTTCTGGG
CAGTCTGTGGCGTGCGCAACCCTTCGCCATAATCGATGACGGCACCATCGAGGGGCGTGAGCGGGCAGCGCGCGTATTGG
CAAATCTCAAGGAACAGCAATTGCAGCCGGTCTTCACCGATACCTATCGGCCCGGCCTTGATAAGCAGAATGCGCTGGTC
TCACGCTTGCGGCGGGCGGGCGCCACCCATGTCTATGTGGGCGGCGAGCGCGACGATATCGCGGCGATTGGCGCAAGTGC
AGCAGCCTTGAACTATCCGCTTGTCATTGCAGGCGGGAGCCTGCTCGACGCTGCGCCCGGCCCCCATCCCCTCGCGCAGG
GCACATTGATGATCGCCCCCGTTCGCCCGTCGGATTTATCGACAGCAAAACCGGCCATCGACGCATTGCATCAGGCCGAT
AAACTTGCTGATTCCTATGCCATTATCGGCTATGCGAGCGTGGAAATCGCCGACGAGGCTATCAAACGCACAGATGAACA
GAAGCAGCCTATCATCAATCTTCTGCGCAGCGGCTCCTTTGAAACCGCTCTGGGCACCATCAAATTTGATGAAAACGGCA
TCAGGACGGACAATCCCAACCGTTTGCAGCGCTTCGACGGCAAACGGTTCATCCTAACCGACAAATAG

Upstream 100 bases:

>100_bases
CTTTCGATTCCACCGTCGATACGGAGGAGATGATGTTATTTACCGTTTGCACGTCAACACAGACCCGTATTCCACAGACA
GTTACCAGAGCTGACCCCAA

Downstream 100 bases:

>100_bases
GCCGACAAGGCTCGCCGCTATCACCAGTCCGGTCATCAGGCTCATCGTGGACCAGAACAGCACCAGCGCGGCATCGATAT
CCCTCGCCTCGGCCTCGCGT

Product: extracellular ligand-binding receptor

Products: ADP; phosphate; L-leucine [Cytoplasm]; ADP; L-valine [Cytoplasm]; L-iso-leucine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 395; Mature: 394

Protein sequence:

>395_residues
MPASITGIAQRRGNRYPLFYQIATNCLKKTDHVIAPGKCPATVLLTSIVLSWLASGALAQAEDVRVGLAAPLSGTFAPLG
NQLVEGARAAAGTGVQLTVFDDRCDAEGGKEAAERFVEQNIRIATGFLCPEALETALPILSQRNIPIIISDVSELTLAER
RAASPLPTFRLTTGLNKETQATGSFLGSLWRAQPFAIIDDGTIEGRERAARVLANLKEQQLQPVFTDTYRPGLDKQNALV
SRLRRAGATHVYVGGERDDIAAIGASAAALNYPLVIAGGSLLDAAPGPHPLAQGTLMIAPVRPSDLSTAKPAIDALHQAD
KLADSYAIIGYASVEIADEAIKRTDEQKQPIINLLRSGSFETALGTIKFDENGIRTDNPNRLQRFDGKRFILTDK

Sequences:

>Translated_395_residues
MPASITGIAQRRGNRYPLFYQIATNCLKKTDHVIAPGKCPATVLLTSIVLSWLASGALAQAEDVRVGLAAPLSGTFAPLG
NQLVEGARAAAGTGVQLTVFDDRCDAEGGKEAAERFVEQNIRIATGFLCPEALETALPILSQRNIPIIISDVSELTLAER
RAASPLPTFRLTTGLNKETQATGSFLGSLWRAQPFAIIDDGTIEGRERAARVLANLKEQQLQPVFTDTYRPGLDKQNALV
SRLRRAGATHVYVGGERDDIAAIGASAAALNYPLVIAGGSLLDAAPGPHPLAQGTLMIAPVRPSDLSTAKPAIDALHQAD
KLADSYAIIGYASVEIADEAIKRTDEQKQPIINLLRSGSFETALGTIKFDENGIRTDNPNRLQRFDGKRFILTDK
>Mature_394_residues
PASITGIAQRRGNRYPLFYQIATNCLKKTDHVIAPGKCPATVLLTSIVLSWLASGALAQAEDVRVGLAAPLSGTFAPLGN
QLVEGARAAAGTGVQLTVFDDRCDAEGGKEAAERFVEQNIRIATGFLCPEALETALPILSQRNIPIIISDVSELTLAERR
AASPLPTFRLTTGLNKETQATGSFLGSLWRAQPFAIIDDGTIEGRERAARVLANLKEQQLQPVFTDTYRPGLDKQNALVS
RLRRAGATHVYVGGERDDIAAIGASAAALNYPLVIAGGSLLDAAPGPHPLAQGTLMIAPVRPSDLSTAKPAIDALHQADK
LADSYAIIGYASVEIADEAIKRTDEQKQPIINLLRSGSFETALGTIKFDENGIRTDNPNRLQRFDGKRFILTDK

Specific function: Component of an amino-acid transport system (Potential) [H]

COG id: COG0683

COG function: function code E; ABC-type branched-chain amino acid transport systems, periplasmic component

Gene ontology:

Cell location: Periplasmic Protein [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the leucine-binding protein family [H]

Homologues:

Organism=Escherichia coli, GI48994941, Length=343, Percent_Identity=27.1137026239067, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI1789867, Length=341, Percent_Identity=25.8064516129032, Blast_Score=78, Evalue=9e-16,

Paralogues:

None

Copy number: 10140 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 8822 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 8,000 Molecules/Cell In: Glucose minimal med

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001828
- InterPro:   IPR000709 [H]

Pfam domain/function: PF01094 ANF_receptor [H]

EC number: NA

Molecular weight: Translated: 42229; Mature: 42097

Theoretical pI: Translated: 6.93; Mature: 6.93

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
0.5 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
0.3 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPASITGIAQRRGNRYPLFYQIATNCLKKTDHVIAPGKCPATVLLTSIVLSWLASGALAQ
CCCCHHHHHHHCCCCCCCHHHHHHHHHHHCCCEECCCCCCHHHHHHHHHHHHHHCCCCCC
AEDVRVGLAAPLSGTFAPLGNQLVEGARAAAGTGVQLTVFDDRCDAEGGKEAAERFVEQN
HHHCEEEEECCCCCCHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCHHHHHHHHHCC
IRIATGFLCPEALETALPILSQRNIPIIISDVSELTLAERRAASPLPTFRLTTGLNKETQ
CEEEECCCCHHHHHHHHHHHHCCCCCEEEECHHHHHHHHHHCCCCCCCEEEECCCCCHHH
ATGSFLGSLWRAQPFAIIDDGTIEGRERAARVLANLKEQQLQPVFTDTYRPGLDKQNALV
HHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHHHHHHHCCCHHHHCCCCCCCHHHHHH
SRLRRAGATHVYVGGERDDIAAIGASAAALNYPLVIAGGSLLDAAPGPHPLAQGTLMIAP
HHHHHCCCCEEEECCCCCCHHHHCCHHHHHCCCEEEECCCEECCCCCCCCCCCCEEEEEE
VRPSDLSTAKPAIDALHQADKLADSYAIIGYASVEIADEAIKRTDEQKQPIINLLRSGSF
CCCCCCCCCHHHHHHHHHHHHHHCCEEEEEEEHHHHHHHHHHHCCHHHHHHHHHHHCCCC
ETALGTIKFDENGIRTDNPNRLQRFDGKRFILTDK
CCEEEEEEECCCCCCCCCCHHHHHCCCCEEEEECC
>Mature Secondary Structure 
PASITGIAQRRGNRYPLFYQIATNCLKKTDHVIAPGKCPATVLLTSIVLSWLASGALAQ
CCCHHHHHHHCCCCCCCHHHHHHHHHHHCCCEECCCCCCHHHHHHHHHHHHHHCCCCCC
AEDVRVGLAAPLSGTFAPLGNQLVEGARAAAGTGVQLTVFDDRCDAEGGKEAAERFVEQN
HHHCEEEEECCCCCCHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCHHHHHHHHHCC
IRIATGFLCPEALETALPILSQRNIPIIISDVSELTLAERRAASPLPTFRLTTGLNKETQ
CEEEECCCCHHHHHHHHHHHHCCCCCEEEECHHHHHHHHHHCCCCCCCEEEECCCCCHHH
ATGSFLGSLWRAQPFAIIDDGTIEGRERAARVLANLKEQQLQPVFTDTYRPGLDKQNALV
HHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHHHHHHHCCCHHHHCCCCCCCHHHHHH
SRLRRAGATHVYVGGERDDIAAIGASAAALNYPLVIAGGSLLDAAPGPHPLAQGTLMIAP
HHHHHCCCCEEEECCCCCCHHHHCCHHHHHCCCEEEECCCEECCCCCCCCCCCCEEEEEE
VRPSDLSTAKPAIDALHQADKLADSYAIIGYASVEIADEAIKRTDEQKQPIINLLRSGSF
CCCCCCCCCHHHHHHHHHHHHHHCCEEEEEEEHHHHHHHHHHHCCHHHHHHHHHHHCCCC
ETALGTIKFDENGIRTDNPNRLQRFDGKRFILTDK
CCEEEEEEECCCCCCCCCCHHHHHCCCCEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-leucine [Periplasm]; H2O; ATP; L-valine [Periplasm]; L-iso-leucine [Periplasm] [C]

Specific reaction: ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] ATP + L-valine [Periplasm] + H2O = ADP + phosphate + L-valine [Cytoplasm] ATP + L-iso-leucine [Periplasm] + H2O = ADP + phosphate + L-iso-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11756688 [H]