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The map label for this gene is lexA

Identifier: 225852643

GI number: 225852643

Start: 1141482

End: 1142204

Strand: Direct

Name: lexA

Synonym: BMEA_A1188

Alternate gene names: 225852643

Gene position: 1141482-1142204 (Clockwise)

Preceding gene: 225852642

Following gene: 225852645

Centisome position: 53.7

GC content: 57.95

Gene sequence:

>723_bases
ATGTTGACCCGTAAGCAGCACGAGCTTCTGCTGTTCATTCATGAACGTCTTAAAGAAACGGGCATTCCTCCTTCTTTTGA
CGAGATGAAGGAAGCTCTGGACCTTGCGTCCAAATCAGGTATTCACCGCCTCATCACGGCGCTGGAAGAGCGTGGCTTCA
TTCGCAGGCTGCCCAACCGGGCGCGCGCACTCGAAGTGCTCCGCCTGCCGGATTCGATCGCTCCCGGTCTCAGCCCGCAG
AAGAAATTCGCACCCAGTGTAATTGAAGGAAGCCTCGGAAAAGTAGCTTCCGTTCAGCCCGTGCGCCCTGCCCCGGCTCC
ACAAAACAGCGAGGCGCCAGCCACTGTCTCTGTGCCGGTCATGGGGCGTATTGCTGCCGGTGTGCCGATCTCTGCCATCC
AGAACCAGACCCATATGCTGAGCCTGCCGCCGGAAATGATCGGCGCGGGCGAACATTATGCGCTGGAAGTCAAAGGCGAT
TCCATGATCGATGCCGGAATTTTCGACGGCGATACGGTCATCATCAAACGCGGCGATACGGCCAATCCGGGAGAAATTGT
CGTGGCACTGGTGGATGAAGAGGAAGCAACCTTGAAGCGCTTCCGCCGCGAGGGCGCTTCCATCGCACTGGAAGCAGCCA
ATCCGGCCTATGAAACCCGGATTTTCGGCCCTGATCGGGTGCATGTTCAGGGGAAGCTTGTGGGCTTGATCCGCCGCTAT
TGA

Upstream 100 bases:

>100_bases
CCCCGATTAATCATTTTAAAACCATTGCAGAACAAGACTGGAACATATAGTGTTCGTTCTGGTTTTGTTTTTCTCGATTC
TGGGCCGGAGGGTATAGCGC

Downstream 100 bases:

>100_bases
GCGGAACCGTTTCTGCACCGGCCTTGTGTTTCACCAGATGCTGAACAAGCAGATGCAGCGGAACTGGATGACAGTTTCTT
CTTGTATTCCCGATCAGGCA

Product: LexA repressor

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 240; Mature: 240

Protein sequence:

>240_residues
MLTRKQHELLLFIHERLKETGIPPSFDEMKEALDLASKSGIHRLITALEERGFIRRLPNRARALEVLRLPDSIAPGLSPQ
KKFAPSVIEGSLGKVASVQPVRPAPAPQNSEAPATVSVPVMGRIAAGVPISAIQNQTHMLSLPPEMIGAGEHYALEVKGD
SMIDAGIFDGDTVIIKRGDTANPGEIVVALVDEEEATLKRFRREGASIALEAANPAYETRIFGPDRVHVQGKLVGLIRRY

Sequences:

>Translated_240_residues
MLTRKQHELLLFIHERLKETGIPPSFDEMKEALDLASKSGIHRLITALEERGFIRRLPNRARALEVLRLPDSIAPGLSPQ
KKFAPSVIEGSLGKVASVQPVRPAPAPQNSEAPATVSVPVMGRIAAGVPISAIQNQTHMLSLPPEMIGAGEHYALEVKGD
SMIDAGIFDGDTVIIKRGDTANPGEIVVALVDEEEATLKRFRREGASIALEAANPAYETRIFGPDRVHVQGKLVGLIRRY
>Mature_240_residues
MLTRKQHELLLFIHERLKETGIPPSFDEMKEALDLASKSGIHRLITALEERGFIRRLPNRARALEVLRLPDSIAPGLSPQ
KKFAPSVIEGSLGKVASVQPVRPAPAPQNSEAPATVSVPVMGRIAAGVPISAIQNQTHMLSLPPEMIGAGEHYALEVKGD
SMIDAGIFDGDTVIIKRGDTANPGEIVVALVDEEEATLKRFRREGASIALEAANPAYETRIFGPDRVHVQGKLVGLIRRY

Specific function: Represses a number of genes involved in the response to DNA damage (SOS response), including recA and lexA. In the presence of single-stranded DNA, recA interacts with lexA causing an autocatalytic cleavage which disrupts the DNA-binding part of lexA, lea

COG id: COG1974

COG function: function code KT; SOS-response transcriptional repressors (RecA-mediated autopeptidases)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S24 family

Homologues:

Organism=Escherichia coli, GI1790476, Length=239, Percent_Identity=24.2677824267782, Blast_Score=72, Evalue=4e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LEXA_BRUA1 (B2S5Z4)

Other databases:

- EMBL:   CP000887
- RefSeq:   YP_001935065.1
- ProteinModelPortal:   B2S5Z4
- SMR:   B2S5Z4
- GeneID:   6328633
- GenomeReviews:   CP000887_GR
- KEGG:   bmc:BAbS19_I10840
- HOGENOM:   HBG679610
- OMA:   LMFIHER
- ProtClustDB:   PRK00215
- GO:   GO:0006508
- GO:   GO:0006350
- HAMAP:   MF_00015
- InterPro:   IPR006199
- InterPro:   IPR006200
- InterPro:   IPR006197
- InterPro:   IPR019759
- InterPro:   IPR015927
- InterPro:   IPR011056
- InterPro:   IPR011991
- Gene3D:   G3DSA:2.10.109.10
- Gene3D:   G3DSA:1.10.10.10
- PRINTS:   PR00726
- TIGRFAMs:   TIGR00498

Pfam domain/function: PF01726 LexA_DNA_bind; PF00717 Peptidase_S24; SSF51306 Pept_S24_S26_C

EC number: =3.4.21.88

Molecular weight: Translated: 26071; Mature: 26071

Theoretical pI: Translated: 7.94; Mature: 7.94

Prosite motif: NA

Important sites: ACT_SITE 161-161 ACT_SITE 199-199

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLTRKQHELLLFIHERLKETGIPPSFDEMKEALDLASKSGIHRLITALEERGFIRRLPNR
CCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHCCHHHHCCCH
ARALEVLRLPDSIAPGLSPQKKFAPSVIEGSLGKVASVQPVRPAPAPQNSEAPATVSVPV
HHHHHHHCCCHHHCCCCCCHHHCCCHHHHCCCCCCCCCCCCCCCCCCCCCCCCCEEECCC
MGRIAAGVPISAIQNQTHMLSLPPEMIGAGEHYALEVKGDSMIDAGIFDGDTVIIKRGDT
HHHHHCCCCHHHHCCCCEEEECCHHHHCCCCEEEEEECCCCEEECCEECCCEEEEECCCC
ANPGEIVVALVDEEEATLKRFRREGASIALEAANPAYETRIFGPDRVHVQGKLVGLIRRY
CCCCEEEEEEECCCHHHHHHHHHCCCEEEEECCCCCCEEEECCCCEEEECHHHHHHHHCC
>Mature Secondary Structure
MLTRKQHELLLFIHERLKETGIPPSFDEMKEALDLASKSGIHRLITALEERGFIRRLPNR
CCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHCCHHHHCCCH
ARALEVLRLPDSIAPGLSPQKKFAPSVIEGSLGKVASVQPVRPAPAPQNSEAPATVSVPV
HHHHHHHCCCHHHCCCCCCHHHCCCHHHHCCCCCCCCCCCCCCCCCCCCCCCCCEEECCC
MGRIAAGVPISAIQNQTHMLSLPPEMIGAGEHYALEVKGDSMIDAGIFDGDTVIIKRGDT
HHHHHCCCCHHHHCCCCEEEECCHHHHCCCCEEEEEECCCCEEECCEECCCEEEEECCCC
ANPGEIVVALVDEEEATLKRFRREGASIALEAANPAYETRIFGPDRVHVQGKLVGLIRRY
CCCCEEEEEEECCCHHHHHHHHHCCCEEEEECCCCCCEEEECCCCEEEECHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA