Click here to switch to the map view.

The map label for this gene is gltX

Identifier: 225852645

GI number: 225852645

Start: 1144949

End: 1146370

Strand: Direct

Name: gltX

Synonym: BMEA_A1191

Alternate gene names: 225852645

Gene position: 1144949-1146370 (Clockwise)

Preceding gene: 225852643

Following gene: 225852646

Centisome position: 53.86

GC content: 59.92

Gene sequence:

>1422_bases
ATGTCGAAACCGGTTATTACCCGTTTTGCCCCCTCGCCGACGGGTTATCTTCACATTGGCGGAGCCCGCACCGCTCTGTT
CAACTGGCTTTACGCCAAGCATTGCGGCGGCAAGATGCTGCTGCGCATCGAAGATACCGACCGCGAACGTTCCACGGAGG
CTGCAACGGCAGCCATTCTGGACGGCCTGACCTGGCTTGGCCTGGATTGGGATGGCGAGGCAATCTCGCAGTTCGAGCGC
GCGCCGCGCCACCGCGAAGTGGCCGAAGAGCTGGTTGCAAACGGCAAGGCCTATTATTGCTATGCAAGCCCGGAAGAGCT
GGAAGAAATGCGCGAGAAGGCACGCGCCGAAGGCCGCCCGCCGCGTTATGACGGGCGCTGGCGCGATCGCGATCCCTCCG
AGGCACCGGCAGGCGTAAAGCCGGTCATCCGAATCAAGGCGCCTCGGGATGGCGAAACCGTCGTGCACGATGCCGTGCAG
GGCGATGTTCGCTTCCCGAACAAGGATCTGGACGATTTCATCATTCTCCGTTCGGATGGCACACCGACCTATATGCACGC
TGTCGTGGTCGATGACCACGACATGGGCGTCACCCATATCATTCGCGGCGACGACCATCTCACCAACGCCGCGCGCCAGA
CGATCATCTATAATGCCATGGGCTGGGACGTACCGCAGATGTCGCATATCCCCCTCATCCATGGCGCCGATGGCGCAAAG
CTTTCCAAGCGTCACGGCGCGCTTGGCGTGGATGCCTATCGGGCCATAGGCTATCTGCCGGCCGCCCTGCGCAACTATCT
CGTCCGCCTCGGCTGGAGCCATGGCGACGACGAAATCATGTCAACCGAACAGATGATCGAATGGTTCGATGTGAAGGACA
TCAACAAGGGCGCTGCCCGCTTTGATTTCCAGAAGCTGGAGGCAATCAACGGTCTTTATATGCGTTCAAGCGACGATCAG
GCGCTGTTTGATGCGCTCGTTGCCGTTTTGCCGGAAATTGAAGGCGGCAAGGAGCTGGCCGAAGCCCTGGACGACAAAGG
CCGCGCACAACTTCTGCTCGCTATGCCGGGGCTGAAGGAGCGCGCAAAGACGCTTGTTGAGCTTGCCGATGGTGCAAAGT
TCATTTTTGCCAGCCGCCCGCTCGCGCTGGACGAAAAAGCCGCATCCTTGCTCAACGATGAAGGCCGCGCTGTACTGAAG
CCGGTCTATCCTGTTCTGGAAGCAATGGGCGAATGGACGGCTGAATCGCTTGATGCGGCAATCCGCGCCCATGCGGAAGC
CGAAGGGCTGAAACTTGGCAAGATTGCGCAACCCTTACGCGCCGCCCTGACTGGGCGCGCCACTTCTCCGGGTGTATTCG
ACGTATTGGTGGTGCTTGGAAGAGAGGAATCCCTCGCCCGCATCGGCGATCAAATCGGTTAA

Upstream 100 bases:

>100_bases
CCTGCCCATTAACAATATGCGGCATCAAAAATTCAGGAATGCGCCTCTGCGCGGCATTTGCCACTGCTTTCAGACGGGCG
CGCGACATAGTGGAGCTATC

Downstream 100 bases:

>100_bases
GCTGGGCCTATTCACGCTGACGTGACAAAACCCGCGAAAATAGTCCAAATTGCGCATTGCATCGGAGAAACTTTGGGATA
GTTTGGCGGGCAAAATCGTT

Product: glutamyl-tRNA synthetase

Products: NA

Alternate protein names: Glutamate--tRNA ligase 1; GluRS 1

Number of amino acids: Translated: 473; Mature: 472

Protein sequence:

>473_residues
MSKPVITRFAPSPTGYLHIGGARTALFNWLYAKHCGGKMLLRIEDTDRERSTEAATAAILDGLTWLGLDWDGEAISQFER
APRHREVAEELVANGKAYYCYASPEELEEMREKARAEGRPPRYDGRWRDRDPSEAPAGVKPVIRIKAPRDGETVVHDAVQ
GDVRFPNKDLDDFIILRSDGTPTYMHAVVVDDHDMGVTHIIRGDDHLTNAARQTIIYNAMGWDVPQMSHIPLIHGADGAK
LSKRHGALGVDAYRAIGYLPAALRNYLVRLGWSHGDDEIMSTEQMIEWFDVKDINKGAARFDFQKLEAINGLYMRSSDDQ
ALFDALVAVLPEIEGGKELAEALDDKGRAQLLLAMPGLKERAKTLVELADGAKFIFASRPLALDEKAASLLNDEGRAVLK
PVYPVLEAMGEWTAESLDAAIRAHAEAEGLKLGKIAQPLRAALTGRATSPGVFDVLVVLGREESLARIGDQIG

Sequences:

>Translated_473_residues
MSKPVITRFAPSPTGYLHIGGARTALFNWLYAKHCGGKMLLRIEDTDRERSTEAATAAILDGLTWLGLDWDGEAISQFER
APRHREVAEELVANGKAYYCYASPEELEEMREKARAEGRPPRYDGRWRDRDPSEAPAGVKPVIRIKAPRDGETVVHDAVQ
GDVRFPNKDLDDFIILRSDGTPTYMHAVVVDDHDMGVTHIIRGDDHLTNAARQTIIYNAMGWDVPQMSHIPLIHGADGAK
LSKRHGALGVDAYRAIGYLPAALRNYLVRLGWSHGDDEIMSTEQMIEWFDVKDINKGAARFDFQKLEAINGLYMRSSDDQ
ALFDALVAVLPEIEGGKELAEALDDKGRAQLLLAMPGLKERAKTLVELADGAKFIFASRPLALDEKAASLLNDEGRAVLK
PVYPVLEAMGEWTAESLDAAIRAHAEAEGLKLGKIAQPLRAALTGRATSPGVFDVLVVLGREESLARIGDQIG
>Mature_472_residues
SKPVITRFAPSPTGYLHIGGARTALFNWLYAKHCGGKMLLRIEDTDRERSTEAATAAILDGLTWLGLDWDGEAISQFERA
PRHREVAEELVANGKAYYCYASPEELEEMREKARAEGRPPRYDGRWRDRDPSEAPAGVKPVIRIKAPRDGETVVHDAVQG
DVRFPNKDLDDFIILRSDGTPTYMHAVVVDDHDMGVTHIIRGDDHLTNAARQTIIYNAMGWDVPQMSHIPLIHGADGAKL
SKRHGALGVDAYRAIGYLPAALRNYLVRLGWSHGDDEIMSTEQMIEWFDVKDINKGAARFDFQKLEAINGLYMRSSDDQA
LFDALVAVLPEIEGGKELAEALDDKGRAQLLLAMPGLKERAKTLVELADGAKFIFASRPLALDEKAASLLNDEGRAVLKP
VYPVLEAMGEWTAESLDAAIRAHAEAEGLKLGKIAQPLRAALTGRATSPGVFDVLVVLGREESLARIGDQIG

Specific function: Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction:glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu)

COG id: COG0008

COG function: function code J; Glutamyl- and glutaminyl-tRNA synthetases

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI134288884, Length=489, Percent_Identity=31.9018404907975, Blast_Score=228, Evalue=1e-59,
Organism=Escherichia coli, GI1788743, Length=466, Percent_Identity=43.3476394849785, Blast_Score=376, Evalue=1e-105,
Organism=Escherichia coli, GI226510925, Length=283, Percent_Identity=37.1024734982332, Blast_Score=139, Evalue=5e-34,
Organism=Escherichia coli, GI1786895, Length=347, Percent_Identity=23.9193083573487, Blast_Score=69, Evalue=5e-13,
Organism=Caenorhabditis elegans, GI71993394, Length=487, Percent_Identity=30.8008213552361, Blast_Score=205, Evalue=5e-53,
Organism=Caenorhabditis elegans, GI17543332, Length=107, Percent_Identity=37.3831775700935, Blast_Score=67, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6324540, Length=495, Percent_Identity=31.9191919191919, Blast_Score=238, Evalue=2e-63,
Organism=Saccharomyces cerevisiae, GI6321192, Length=335, Percent_Identity=26.865671641791, Blast_Score=80, Evalue=8e-16,
Organism=Saccharomyces cerevisiae, GI6324742, Length=130, Percent_Identity=36.9230769230769, Blast_Score=77, Evalue=5e-15,
Organism=Drosophila melanogaster, GI24665321, Length=488, Percent_Identity=31.9672131147541, Blast_Score=250, Evalue=2e-66,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYE1_BRUME (Q8YHG4)

Other databases:

- EMBL:   AE008917
- PIR:   AG3356
- RefSeq:   NP_539754.1
- ProteinModelPortal:   Q8YHG4
- SMR:   Q8YHG4
- GeneID:   1196548
- GenomeReviews:   AE008917_GR
- KEGG:   bme:BMEI0837
- NMPDR:   fig|224914.1.peg.836
- HOGENOM:   HBG628189
- OMA:   MAHIPLI
- PhylomeDB:   Q8YHG4
- ProtClustDB:   PRK01406
- BioCyc:   BMEL224914:BMEI0837-MONOMER
- BRENDA:   6.1.1.17
- GO:   GO:0005737
- HAMAP:   MF_00022_B
- InterPro:   IPR008925
- InterPro:   IPR020751
- InterPro:   IPR001412
- InterPro:   IPR004527
- InterPro:   IPR000924
- InterPro:   IPR020061
- InterPro:   IPR020058
- InterPro:   IPR014729
- Gene3D:   G3DSA:1.10.1160.10
- Gene3D:   G3DSA:3.40.50.620
- Gene3D:   G3DSA:1.10.10.350
- PANTHER:   PTHR10119
- PRINTS:   PR00987
- TIGRFAMs:   TIGR00464

Pfam domain/function: PF00749 tRNA-synt_1c; SSF48163 tRNA-synt_bind

EC number: =6.1.1.17

Molecular weight: Translated: 52064; Mature: 51933

Theoretical pI: Translated: 5.28; Mature: 5.28

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: BINDING 243-243

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKPVITRFAPSPTGYLHIGGARTALFNWLYAKHCGGKMLLRIEDTDRERSTEAATAAIL
CCCCCEEEECCCCCCEEEECCHHHHHHHHHHHHHCCCEEEEEEECCCCCHHHHHHHHHHH
DGLTWLGLDWDGEAISQFERAPRHREVAEELVANGKAYYCYASPEELEEMREKARAEGRP
CCCEEEECCCCHHHHHHHHHCCCHHHHHHHHHHCCCEEEEECCHHHHHHHHHHHHHCCCC
PRYDGRWRDRDPSEAPAGVKPVIRIKAPRDGETVVHDAVQGDVRFPNKDLDDFIILRSDG
CCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCHHHHHHHCCCCCCCCCCCCCEEEEECCC
TPTYMHAVVVDDHDMGVTHIIRGDDHLTNAARQTIIYNAMGWDVPQMSHIPLIHGADGAK
CCEEEEEEEEECCCCCEEEEECCCHHHHHHHHHHEEEECCCCCCCCCCCCCEEECCCCCC
LSKRHGALGVDAYRAIGYLPAALRNYLVRLGWSHGDDEIMSTEQMIEWFDVKDINKGAAR
HHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCCHHH
FDFQKLEAINGLYMRSSDDQALFDALVAVLPEIEGGKELAEALDDKGRAQLLLAMPGLKE
HHHHHHHHHCCEEEECCCCHHHHHHHHHHHCCCCCHHHHHHHHCCCCCEEEEEECCCHHH
RAKTLVELADGAKFIFASRPLALDEKAASLLNDEGRAVLKPVYPVLEAMGEWTAESLDAA
HHHHHHHHHCCCEEEEECCCCCCCHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
IRAHAEAEGLKLGKIAQPLRAALTGRATSPGVFDVLVVLGREESLARIGDQIG
HHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCHHHHHHHHCCC
>Mature Secondary Structure 
SKPVITRFAPSPTGYLHIGGARTALFNWLYAKHCGGKMLLRIEDTDRERSTEAATAAIL
CCCCEEEECCCCCCEEEECCHHHHHHHHHHHHHCCCEEEEEEECCCCCHHHHHHHHHHH
DGLTWLGLDWDGEAISQFERAPRHREVAEELVANGKAYYCYASPEELEEMREKARAEGRP
CCCEEEECCCCHHHHHHHHHCCCHHHHHHHHHHCCCEEEEECCHHHHHHHHHHHHHCCCC
PRYDGRWRDRDPSEAPAGVKPVIRIKAPRDGETVVHDAVQGDVRFPNKDLDDFIILRSDG
CCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCHHHHHHHCCCCCCCCCCCCCEEEEECCC
TPTYMHAVVVDDHDMGVTHIIRGDDHLTNAARQTIIYNAMGWDVPQMSHIPLIHGADGAK
CCEEEEEEEEECCCCCEEEEECCCHHHHHHHHHHEEEECCCCCCCCCCCCCEEECCCCCC
LSKRHGALGVDAYRAIGYLPAALRNYLVRLGWSHGDDEIMSTEQMIEWFDVKDINKGAAR
HHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCCHHH
FDFQKLEAINGLYMRSSDDQALFDALVAVLPEIEGGKELAEALDDKGRAQLLLAMPGLKE
HHHHHHHHHCCEEEECCCCHHHHHHHHHHHCCCCCHHHHHHHHCCCCCEEEEEECCCHHH
RAKTLVELADGAKFIFASRPLALDEKAASLLNDEGRAVLKPVYPVLEAMGEWTAESLDAA
HHHHHHHHHCCCEEEEECCCCCCCHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
IRAHAEAEGLKLGKIAQPLRAALTGRATSPGVFDVLVVLGREESLARIGDQIG
HHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11756688