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The map label for this gene is mrcA [H]

Identifier: 225852427

GI number: 225852427

Start: 909752

End: 912211

Strand: Direct

Name: mrcA [H]

Synonym: BMEA_A0954

Alternate gene names: 225852427

Gene position: 909752-912211 (Clockwise)

Preceding gene: 225852426

Following gene: 225852429

Centisome position: 42.8

GC content: 58.29

Gene sequence:

>2460_bases
ATGGTAAGGCTTATCGGATATTTCTTCGGGATAGGCACCGTTTTGATGCTGTTGGTGGCAGGCGGTGCGGCGCTTTATAT
TGGAAGCCTTGCGAAGGATTTGCCGGATTATGAGGTGCTCGCCAAGTACGAGCCACCGGTGATGACGCGAGTTCATGCTT
CCGATGGCAGCCTGATGGCCGAGTTTGCCCGTGAGCGACGCCTTTATCTGCCGATTCAGGCCGTGCCGAACCGCGTGAAG
GCGGCTTTCGTTTCCGCCGAAGACAAGACCTTTTATGAGCATCATGGCCTCGATTTCGGTGGCCTTGCGCGTGCGGTGGT
CACGAATCTGAAGAATATAGGTTCTGGCCGCCGTCCGGTCGGCGCCTCCACCATCACGCAACAGGTGGCGAAGAACTTCC
TGCTGAACTCCAAGCAGACCTATGATCGCAAGATCAAGGAAGCCATCCTTGCGATGCGCATTGAGCAGGCATATTCCAAG
GATCGCATTCTTGAGCTTTACCTGAACGAGATTTTCTTTGGTCTCGGTTCCTATGGCATTGCCAGTGCGGCACTGACCTA
TTTCGACAAGTCGGTGGGCGAACTGACCATTGCGGAAAGTGCCTATCTTGCGGCCCTGCCCAAGGGGCCGAACAATTACC
ATCCCTTCCGCCAGCCCGAGCGCGCCATCGAGCGCCGCAACTGGGTGATCGACCGCATGGCCGAGAATGGCTATATCACG
ACTGCCGAAGCGGAAGACGCAAAGAAGCAGCCCCTTGGCGTCACCCCCCGCACGGCTACCCATTATCTCTTTGCTTCCGA
ATATTTCACCGAAGAAGTGCGCCGCCAGATCATTCAGAAATACGGTGTCGATGCGCTTTACGAAGGCGGCTTGTCGGTTC
GCACGACCCTCAATCCCAAATTGCAGCTTGAAGCGCGCAAATCCTTGCAGGCGGCATTGATACGCTATGACGAGGCGCGC
GGCTGGCGCGGCCCGTTGAAAAATGTCGAGCTTGGCGGCGATTGGGGTACGGCTTTCGGCAATATGGAGCCCTATTCGGA
TGTGCCGGAATGGCAGCTTGCCGTCGTCCTGAATGTTACGCCTGCCGGTGCTGATATCGGCCTCCAGCCGCAGTTTGACG
CGTCCGGTTCACGCAGCAAGGAGCGCAAGCGCGCTTTCATCGCTGCCGACGATATGAAATGGGCAATGCGCATCGTCAAT
ATCGGCGGAAAGCGCACCAGTGCCAAGTCGCCGGAAGGCGTGCTGAAGCCGGGCGATATTATCTATGTGTCAAAGACGGG
CGATTCCCGGTATAGTCTCCAGCAGCCGCCGAAGCTTGAAGGAGCACTGGTTGCCATGGACCCCCATACGGGCCGTGTGC
TGGCCATGGTTGGCGGTTTTTCCTTTGGCGAATCGGAGTTTAATCGCGCCACGCAGGCCTATCGCCAGCCGGGTTCGTCC
TTCAAGCCGTTTGTCTATGCGGCTGCACTGGATAATGGCTATACGCCTGCTTCCGTGGTGCTGGACGGGCCGCTGGAAAT
TGACCAGGGCGGGTCGCTCGGTATCTGGGCTCCGAAAAACTTTTCGGGCAAGTTCGCCGGCCCCTCTACGCTGCGCTACG
GCATCGAGCAGTCCCGTAACGTCATGACCGTGCGTCTGGCGCAGGATATGGGCATGAAGCTCGTGGTCGAATATGCCGAG
CGTTTCGGTATCTACGACAAGATGTTGCCTGTTCTCTCCATGGTGCTTGGCGCGGGCGAAACCACGGTGCTTCGTATGGT
GACGGCTTATTCGATTATAGCCAATGGCGGGCAGAGCATCACGCCATCGATGATCGACCGCATTCAGGACCGCTACGGCA
AGACCGTGTTCAAGCATGACAACCGCCAGTGCGAAGGTTGCAATGCGCAGGAATGGGCCAATCAGGACGAGCCGACACTG
ATCGACAATCGCGATCAGGTTCTCGATCCCATGACGGCCTATCAGATCACGTCGATGATGGAAGGTGTCGTGCAGCGCGG
AACGGCACAGATATTGAAGAGCCTGGACCGCCCGCTGGCGGGCAAGACCGGAACGACCAACGAGGAAAAGGATGCCTGGT
TCGTGGGCTTCACACCTGATCTCGTGGTTGGCGTTTTCATGGGATATGATACGCCTACTCCGCTTGGCCGCGGCAATACG
GGCGGTGGCCTTGCCGCGCCGGTGTTCAAGTCCTTCATGGAGCAGGCTTTGGCTCGAACGCCGAAGGTTGATTTCCGTGT
GCCGGAAGGCATGACCGTCATCGCGATCGACCGCAAGACGGGCATGCGCGCCAATCCGGGCGATCCGAATGTCATTATGG
AAGCTTTCAAGCCGGGCACCGGCCCGTCCGACAGCTATTCGGTCATCGGCATGGACACGTTCCGCGAAGGCTCCCCGGTT
GCGCCGCAGTCCCCGCAGGCAACCCGCGCGATCAATTCCGGTTCCGGCGGACTTTACTGA

Upstream 100 bases:

>100_bases
TAAGCCAATTAGTTGCCATGCGGCACAATAGGTTCGAGGGTTGGCAGTTTATGTTTCGACCCTTATCATGTGAATAATCT
GAAGGCAGGATTCCGGGTTT

Downstream 100 bases:

>100_bases
CCCGTCAAGCTTTACACGGCGCCGCGTACTCACTATGGTCCGCGGCGCTTGCGGTTCATTCGCAAGTCAAAGCCTGAACC
CTGAATATTCCGGTTCGGCT

Product: 1A family penicillin-binding protein

Products: NA

Alternate protein names: PBP-1a; PBP1a; Penicillin-insensitive transglycosylase; Peptidoglycan TGase; Penicillin-sensitive transpeptidase; DD-transpeptidase [H]

Number of amino acids: Translated: 819; Mature: 819

Protein sequence:

>819_residues
MVRLIGYFFGIGTVLMLLVAGGAALYIGSLAKDLPDYEVLAKYEPPVMTRVHASDGSLMAEFARERRLYLPIQAVPNRVK
AAFVSAEDKTFYEHHGLDFGGLARAVVTNLKNIGSGRRPVGASTITQQVAKNFLLNSKQTYDRKIKEAILAMRIEQAYSK
DRILELYLNEIFFGLGSYGIASAALTYFDKSVGELTIAESAYLAALPKGPNNYHPFRQPERAIERRNWVIDRMAENGYIT
TAEAEDAKKQPLGVTPRTATHYLFASEYFTEEVRRQIIQKYGVDALYEGGLSVRTTLNPKLQLEARKSLQAALIRYDEAR
GWRGPLKNVELGGDWGTAFGNMEPYSDVPEWQLAVVLNVTPAGADIGLQPQFDASGSRSKERKRAFIAADDMKWAMRIVN
IGGKRTSAKSPEGVLKPGDIIYVSKTGDSRYSLQQPPKLEGALVAMDPHTGRVLAMVGGFSFGESEFNRATQAYRQPGSS
FKPFVYAAALDNGYTPASVVLDGPLEIDQGGSLGIWAPKNFSGKFAGPSTLRYGIEQSRNVMTVRLAQDMGMKLVVEYAE
RFGIYDKMLPVLSMVLGAGETTVLRMVTAYSIIANGGQSITPSMIDRIQDRYGKTVFKHDNRQCEGCNAQEWANQDEPTL
IDNRDQVLDPMTAYQITSMMEGVVQRGTAQILKSLDRPLAGKTGTTNEEKDAWFVGFTPDLVVGVFMGYDTPTPLGRGNT
GGGLAAPVFKSFMEQALARTPKVDFRVPEGMTVIAIDRKTGMRANPGDPNVIMEAFKPGTGPSDSYSVIGMDTFREGSPV
APQSPQATRAINSGSGGLY

Sequences:

>Translated_819_residues
MVRLIGYFFGIGTVLMLLVAGGAALYIGSLAKDLPDYEVLAKYEPPVMTRVHASDGSLMAEFARERRLYLPIQAVPNRVK
AAFVSAEDKTFYEHHGLDFGGLARAVVTNLKNIGSGRRPVGASTITQQVAKNFLLNSKQTYDRKIKEAILAMRIEQAYSK
DRILELYLNEIFFGLGSYGIASAALTYFDKSVGELTIAESAYLAALPKGPNNYHPFRQPERAIERRNWVIDRMAENGYIT
TAEAEDAKKQPLGVTPRTATHYLFASEYFTEEVRRQIIQKYGVDALYEGGLSVRTTLNPKLQLEARKSLQAALIRYDEAR
GWRGPLKNVELGGDWGTAFGNMEPYSDVPEWQLAVVLNVTPAGADIGLQPQFDASGSRSKERKRAFIAADDMKWAMRIVN
IGGKRTSAKSPEGVLKPGDIIYVSKTGDSRYSLQQPPKLEGALVAMDPHTGRVLAMVGGFSFGESEFNRATQAYRQPGSS
FKPFVYAAALDNGYTPASVVLDGPLEIDQGGSLGIWAPKNFSGKFAGPSTLRYGIEQSRNVMTVRLAQDMGMKLVVEYAE
RFGIYDKMLPVLSMVLGAGETTVLRMVTAYSIIANGGQSITPSMIDRIQDRYGKTVFKHDNRQCEGCNAQEWANQDEPTL
IDNRDQVLDPMTAYQITSMMEGVVQRGTAQILKSLDRPLAGKTGTTNEEKDAWFVGFTPDLVVGVFMGYDTPTPLGRGNT
GGGLAAPVFKSFMEQALARTPKVDFRVPEGMTVIAIDRKTGMRANPGDPNVIMEAFKPGTGPSDSYSVIGMDTFREGSPV
APQSPQATRAINSGSGGLY
>Mature_819_residues
MVRLIGYFFGIGTVLMLLVAGGAALYIGSLAKDLPDYEVLAKYEPPVMTRVHASDGSLMAEFARERRLYLPIQAVPNRVK
AAFVSAEDKTFYEHHGLDFGGLARAVVTNLKNIGSGRRPVGASTITQQVAKNFLLNSKQTYDRKIKEAILAMRIEQAYSK
DRILELYLNEIFFGLGSYGIASAALTYFDKSVGELTIAESAYLAALPKGPNNYHPFRQPERAIERRNWVIDRMAENGYIT
TAEAEDAKKQPLGVTPRTATHYLFASEYFTEEVRRQIIQKYGVDALYEGGLSVRTTLNPKLQLEARKSLQAALIRYDEAR
GWRGPLKNVELGGDWGTAFGNMEPYSDVPEWQLAVVLNVTPAGADIGLQPQFDASGSRSKERKRAFIAADDMKWAMRIVN
IGGKRTSAKSPEGVLKPGDIIYVSKTGDSRYSLQQPPKLEGALVAMDPHTGRVLAMVGGFSFGESEFNRATQAYRQPGSS
FKPFVYAAALDNGYTPASVVLDGPLEIDQGGSLGIWAPKNFSGKFAGPSTLRYGIEQSRNVMTVRLAQDMGMKLVVEYAE
RFGIYDKMLPVLSMVLGAGETTVLRMVTAYSIIANGGQSITPSMIDRIQDRYGKTVFKHDNRQCEGCNAQEWANQDEPTL
IDNRDQVLDPMTAYQITSMMEGVVQRGTAQILKSLDRPLAGKTGTTNEEKDAWFVGFTPDLVVGVFMGYDTPTPLGRGNT
GGGLAAPVFKSFMEQALARTPKVDFRVPEGMTVIAIDRKTGMRANPGDPNVIMEAFKPGTGPSDSYSVIGMDTFREGSPV
APQSPQATRAINSGSGGLY

Specific function: Cell wall formation. Synthesis of cross-linked peptidoglycan from the lipid intermediates. The enzyme has a penicillin-insensitive transglycosylase N-terminal domain (formation of linear glycan strands) and a penicillin-sensitive transpeptidase C-terminal

COG id: COG5009

COG function: function code M; Membrane carboxypeptidase/penicillin-binding protein

Gene ontology:

Cell location: Cell inner membrane; Single-pass type II membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the transpeptidase family [H]

Homologues:

Organism=Escherichia coli, GI87082258, Length=847, Percent_Identity=31.9952774498229, Blast_Score=416, Evalue=1e-117,
Organism=Escherichia coli, GI1786343, Length=271, Percent_Identity=33.9483394833948, Blast_Score=147, Evalue=3e-36,
Organism=Escherichia coli, GI1788867, Length=304, Percent_Identity=31.9078947368421, Blast_Score=123, Evalue=4e-29,
Organism=Escherichia coli, GI1789601, Length=156, Percent_Identity=38.4615384615385, Blast_Score=99, Evalue=9e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR001264
- InterPro:   IPR011816
- InterPro:   IPR001460 [H]

Pfam domain/function: PF00912 Transgly; PF00905 Transpeptidase [H]

EC number: 2.4.2.-; 3.4.-.-

Molecular weight: Translated: 89658; Mature: 89658

Theoretical pI: Translated: 8.81; Mature: 8.81

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVRLIGYFFGIGTVLMLLVAGGAALYIGSLAKDLPDYEVLAKYEPPVMTRVHASDGSLMA
CEEHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCHHEECCCCCCEEEEECCCCHHHH
EFARERRLYLPIQAVPNRVKAAFVSAEDKTFYEHHGLDFGGLARAVVTNLKNIGSGRRPV
HHHHCCEEEEEHHHCCCHHHEEEECCCCCHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCC
GASTITQQVAKNFLLNSKQTYDRKIKEAILAMRIEQAYSKDRILELYLNEIFFGLGSYGI
CHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHCCCHHH
ASAALTYFDKSVGELTIAESAYLAALPKGPNNYHPFRQPERAIERRNWVIDRMAENGYIT
HHHHHHHHHHCCCCEEEECCEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEE
TAEAEDAKKQPLGVTPRTATHYLFASEYFTEEVRRQIIQKYGVDALYEGGLSVRTTLNPK
ECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCEEEEEECCCC
LQLEARKSLQAALIRYDEARGWRGPLKNVELGGDWGTAFGNMEPYSDVPEWQLAVVLNVT
EEHHHHHHHHHHHHHHHHCCCCCCCCCCCEECCCCCCCCCCCCCCCCCCCEEEEEEEEEC
PAGADIGLQPQFDASGSRSKERKRAFIAADDMKWAMRIVNIGGKRTSAKSPEGVLKPGDI
CCCCCCCCCCCCCCCCCCCHHHHHEEEEECCHHHHHHHHHCCCCCCCCCCCCCCCCCCCE
IYVSKTGDSRYSLQQPPKLEGALVAMDPHTGRVLAMVGGFSFGESEFNRATQAYRQPGSS
EEEECCCCCCCCCCCCCCCCCEEEEECCCCCEEEEEECCCCCCHHHHHHHHHHHHCCCCC
FKPFVYAAALDNGYTPASVVLDGPLEIDQGGSLGIWAPKNFSGKFAGPSTLRYGIEQSRN
CCCEEEEEECCCCCCCCEEEEECCEEECCCCCEEEECCCCCCCCCCCHHHHHHHHHCCCC
VMTVRLAQDMGMKLVVEYAERFGIYDKMLPVLSMVLGAGETTVLRMVTAYSIIANGGQSI
EEEEEECHHCCHHHHHHHHHHHCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCC
TPSMIDRIQDRYGKTVFKHDNRQCEGCNAQEWANQDEPTLIDNRDQVLDPMTAYQITSMM
CHHHHHHHHHHHCCCCEECCCCCCCCCCHHHHCCCCCCCEECCCHHHHCCHHHHHHHHHH
EGVVQRGTAQILKSLDRPLAGKTGTTNEEKDAWFVGFTPDLVVGVFMGYDTPTPLGRGNT
HHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEECCHHHHHHHHHCCCCCCCCCCCCC
GGGLAAPVFKSFMEQALARTPKVDFRVPEGMTVIAIDRKTGMRANPGDPNVIMEAFKPGT
CCCHHHHHHHHHHHHHHHHCCCCEEECCCCCEEEEEECCCCCCCCCCCCHHHHHHCCCCC
GPSDSYSVIGMDTFREGSPVAPQSPQATRAINSGSGGLY
CCCCCEEEEEECCCCCCCCCCCCCCCHHHHCCCCCCCCC
>Mature Secondary Structure
MVRLIGYFFGIGTVLMLLVAGGAALYIGSLAKDLPDYEVLAKYEPPVMTRVHASDGSLMA
CEEHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCHHEECCCCCCEEEEECCCCHHHH
EFARERRLYLPIQAVPNRVKAAFVSAEDKTFYEHHGLDFGGLARAVVTNLKNIGSGRRPV
HHHHCCEEEEEHHHCCCHHHEEEECCCCCHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCC
GASTITQQVAKNFLLNSKQTYDRKIKEAILAMRIEQAYSKDRILELYLNEIFFGLGSYGI
CHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHCCCHHH
ASAALTYFDKSVGELTIAESAYLAALPKGPNNYHPFRQPERAIERRNWVIDRMAENGYIT
HHHHHHHHHHCCCCEEEECCEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEE
TAEAEDAKKQPLGVTPRTATHYLFASEYFTEEVRRQIIQKYGVDALYEGGLSVRTTLNPK
ECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCEEEEEECCCC
LQLEARKSLQAALIRYDEARGWRGPLKNVELGGDWGTAFGNMEPYSDVPEWQLAVVLNVT
EEHHHHHHHHHHHHHHHHCCCCCCCCCCCEECCCCCCCCCCCCCCCCCCCEEEEEEEEEC
PAGADIGLQPQFDASGSRSKERKRAFIAADDMKWAMRIVNIGGKRTSAKSPEGVLKPGDI
CCCCCCCCCCCCCCCCCCCHHHHHEEEEECCHHHHHHHHHCCCCCCCCCCCCCCCCCCCE
IYVSKTGDSRYSLQQPPKLEGALVAMDPHTGRVLAMVGGFSFGESEFNRATQAYRQPGSS
EEEECCCCCCCCCCCCCCCCCEEEEECCCCCEEEEEECCCCCCHHHHHHHHHHHHCCCCC
FKPFVYAAALDNGYTPASVVLDGPLEIDQGGSLGIWAPKNFSGKFAGPSTLRYGIEQSRN
CCCEEEEEECCCCCCCCEEEEECCEEECCCCCEEEECCCCCCCCCCCHHHHHHHHHCCCC
VMTVRLAQDMGMKLVVEYAERFGIYDKMLPVLSMVLGAGETTVLRMVTAYSIIANGGQSI
EEEEEECHHCCHHHHHHHHHHHCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCC
TPSMIDRIQDRYGKTVFKHDNRQCEGCNAQEWANQDEPTLIDNRDQVLDPMTAYQITSMM
CHHHHHHHHHHHCCCCEECCCCCCCCCCHHHHCCCCCCCEECCCHHHHCCHHHHHHHHHH
EGVVQRGTAQILKSLDRPLAGKTGTTNEEKDAWFVGFTPDLVVGVFMGYDTPTPLGRGNT
HHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEECCHHHHHHHHHCCCCCCCCCCCCC
GGGLAAPVFKSFMEQALARTPKVDFRVPEGMTVIAIDRKTGMRANPGDPNVIMEAFKPGT
CCCHHHHHHHHHHHHHHHHCCCCEEECCCCCEEEEEECCCCCCCCCCCCHHHHHHCCCCC
GPSDSYSVIGMDTFREGSPVAPQSPQATRAINSGSGGLY
CCCCCEEEEEECCCCCCCCCCCCCCCHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferases; Acyltransferases; Aminoacyltransferases [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA