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The map label for this gene is prfB [H]

Identifier: 225852429

GI number: 225852429

Start: 912515

End: 913480

Strand: Direct

Name: prfB [H]

Synonym: BMEA_A0956

Alternate gene names: 225852429

Gene position: 912515-913480 (Clockwise)

Preceding gene: 225852427

Following gene: 225852431

Centisome position: 42.93

GC content: 57.66

Gene sequence:

>966_bases
ATGCGTGAGCGCCAGCAACTGGAAGACAGTATCAATGGCATCAATCACCTCAAGCAGACGCTGAACGATAGTGTCGAACT
CATCGCCATGGGCGAGGAGGAAGGCGACAAGTCGATCGTCGAGGATGCGGAAAAAACCATCCGTGACCTCAAGGATGAGA
TCGACCGCCGCCAGATCGACATGCTCCTCTCCGGCGAAGCCGATGCGAACGACACCTATCTGGAAGTCCATGCGGGCGCA
GGCGGCACGGAAAGCCAGGACTGGGCGTCCATGCTTTTGCGCATGTATACCCGCTGGGCCGAGCGCAACGGGCGCAAGGT
GGAGGTCATGGAAGTACATGAAGGCGAAGAAGCGGGCATCAAGTCCGCGACCATTCTCGTCAAGGGGCACAATTCCTATG
GCATGTTGAAAACCGAATCCGGCGTGCATCGTCTGGTGCGCATTTCGCCTTATGATTCTAACGCGCGCCGTCATACCTCC
TTCGCGAGCATCTGGGTCTATCCTGTCATCGATGACAATATCGAGGTTCAGGTGACGGAGGCGGATGTCCGTATCGACAC
TTATCGCGCTTCTGGCGCGGGCGGGCAGCACGTCAACACGACCGATTCGGCGGTGCGTATCACGCATATTGCAACCGGCA
TCGTGGTGCAGTGTCAGGCGGAACGTTCGCAGCACAAGAACCGTGAAAAGGCCTGGTCCATGCTTCGCGCGCGCCTCTAT
GAAGAGGAGCTGAAAAAGCGCGAAGAGGCTACGGATGCGGCGAATGCCACCAAGACCGATATCGGCTGGGGGCACCAGAT
TCGCTCCTACGTTCTCCAGCCTTATCAGCTTGTGAAAGATTTGCGCACAGGTGTTGAAAGCACCAACCCGCAGGAAGTGC
TGGACGGCGCCGTCACGCCTTTCATGGAGGCCGCTCTTGCCCATCGCATTCAGGGTGGCGGCGAAATGATCGAGGATATC
GATTGA

Upstream 100 bases:

>100_bases
CATCTTTGACTGGGATCAGGCTGTAAAACGTCTGGGCTATCTCAATCAGAGGGCCGAAGATCCCACGCTATGGAATGATG
CTCAGGAAGCTCAGAAGCTG

Downstream 100 bases:

>100_bases
TCCGGGTGAAAGGCTTCAGACAATTAGAGCGCCGATCTGATTGAATCAGATCGGCGCTCTAATCCTTTGTTTTGACACGC
ATCTTTTCCGAAAACCGTTT

Product: peptide chain release factor 2

Products: NA

Alternate protein names: RF-2 [H]

Number of amino acids: Translated: 321; Mature: 321

Protein sequence:

>321_residues
MRERQQLEDSINGINHLKQTLNDSVELIAMGEEEGDKSIVEDAEKTIRDLKDEIDRRQIDMLLSGEADANDTYLEVHAGA
GGTESQDWASMLLRMYTRWAERNGRKVEVMEVHEGEEAGIKSATILVKGHNSYGMLKTESGVHRLVRISPYDSNARRHTS
FASIWVYPVIDDNIEVQVTEADVRIDTYRASGAGGQHVNTTDSAVRITHIATGIVVQCQAERSQHKNREKAWSMLRARLY
EEELKKREEATDAANATKTDIGWGHQIRSYVLQPYQLVKDLRTGVESTNPQEVLDGAVTPFMEAALAHRIQGGGEMIEDI
D

Sequences:

>Translated_321_residues
MRERQQLEDSINGINHLKQTLNDSVELIAMGEEEGDKSIVEDAEKTIRDLKDEIDRRQIDMLLSGEADANDTYLEVHAGA
GGTESQDWASMLLRMYTRWAERNGRKVEVMEVHEGEEAGIKSATILVKGHNSYGMLKTESGVHRLVRISPYDSNARRHTS
FASIWVYPVIDDNIEVQVTEADVRIDTYRASGAGGQHVNTTDSAVRITHIATGIVVQCQAERSQHKNREKAWSMLRARLY
EEELKKREEATDAANATKTDIGWGHQIRSYVLQPYQLVKDLRTGVESTNPQEVLDGAVTPFMEAALAHRIQGGGEMIEDI
D
>Mature_321_residues
MRERQQLEDSINGINHLKQTLNDSVELIAMGEEEGDKSIVEDAEKTIRDLKDEIDRRQIDMLLSGEADANDTYLEVHAGA
GGTESQDWASMLLRMYTRWAERNGRKVEVMEVHEGEEAGIKSATILVKGHNSYGMLKTESGVHRLVRISPYDSNARRHTS
FASIWVYPVIDDNIEVQVTEADVRIDTYRASGAGGQHVNTTDSAVRITHIATGIVVQCQAERSQHKNREKAWSMLRARLY
EEELKKREEATDAANATKTDIGWGHQIRSYVLQPYQLVKDLRTGVESTNPQEVLDGAVTPFMEAALAHRIQGGGEMIEDI
D

Specific function: Peptide chain release factor 2 directs the termination of translation in response to the peptide chain termination codons UGA and UAA [H]

COG id: COG1186

COG function: function code J; Protein chain release factor B

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic/mitochondrial release factor family [H]

Homologues:

Organism=Homo sapiens, GI166795303, Length=248, Percent_Identity=41.9354838709677, Blast_Score=163, Evalue=2e-40,
Organism=Homo sapiens, GI34577120, Length=281, Percent_Identity=36.2989323843416, Blast_Score=144, Evalue=1e-34,
Organism=Homo sapiens, GI166795305, Length=174, Percent_Identity=41.3793103448276, Blast_Score=119, Evalue=4e-27,
Organism=Escherichia coli, GI2367172, Length=305, Percent_Identity=52.7868852459016, Blast_Score=333, Evalue=1e-92,
Organism=Escherichia coli, GI1787462, Length=242, Percent_Identity=43.801652892562, Blast_Score=190, Evalue=1e-49,
Organism=Caenorhabditis elegans, GI17542784, Length=302, Percent_Identity=32.1192052980132, Blast_Score=115, Evalue=3e-26,
Organism=Saccharomyces cerevisiae, GI6321295, Length=275, Percent_Identity=37.0909090909091, Blast_Score=144, Evalue=3e-35,
Organism=Drosophila melanogaster, GI19921226, Length=205, Percent_Identity=41.9512195121951, Blast_Score=151, Evalue=4e-37,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005139
- InterPro:   IPR000352
- InterPro:   IPR020853
- InterPro:   IPR004374 [H]

Pfam domain/function: PF03462 PCRF; PF00472 RF-1 [H]

EC number: NA

Molecular weight: Translated: 36046; Mature: 36046

Theoretical pI: Translated: 4.86; Mature: 4.86

Prosite motif: PS00745 RF_PROK_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRERQQLEDSINGINHLKQTLNDSVELIAMGEEEGDKSIVEDAEKTIRDLKDEIDRRQID
CCHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
MLLSGEADANDTYLEVHAGAGGTESQDWASMLLRMYTRWAERNGRKVEVMEVHEGEEAGI
HHCCCCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCC
KSATILVKGHNSYGMLKTESGVHRLVRISPYDSNARRHTSFASIWVYPVIDDNIEVQVTE
CEEEEEEEECCCCCCEEECCCCEEEEEECCCCCCCHHCCCEEEEEEEEEECCCEEEEEEE
ADVRIDTYRASGAGGQHVNTTDSAVRITHIATGIVVQCQAERSQHKNREKAWSMLRARLY
CCEEEEEEECCCCCCCCCCCCCCCEEEEEECCCEEEEEECHHHHHCCHHHHHHHHHHHHH
EEELKKREEATDAANATKTDIGWGHQIRSYVLQPYQLVKDLRTGVESTNPQEVLDGAVTP
HHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHH
FMEAALAHRIQGGGEMIEDID
HHHHHHHHHHCCCCHHHHCCC
>Mature Secondary Structure
MRERQQLEDSINGINHLKQTLNDSVELIAMGEEEGDKSIVEDAEKTIRDLKDEIDRRQID
CCHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
MLLSGEADANDTYLEVHAGAGGTESQDWASMLLRMYTRWAERNGRKVEVMEVHEGEEAGI
HHCCCCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCC
KSATILVKGHNSYGMLKTESGVHRLVRISPYDSNARRHTSFASIWVYPVIDDNIEVQVTE
CEEEEEEEECCCCCCEEECCCCEEEEEECCCCCCCHHCCCEEEEEEEEEECCCEEEEEEE
ADVRIDTYRASGAGGQHVNTTDSAVRITHIATGIVVQCQAERSQHKNREKAWSMLRARLY
CCEEEEEEECCCCCCCCCCCCCCCEEEEEECCCEEEEEECHHHHHCCHHHHHHHHHHHHH
EEELKKREEATDAANATKTDIGWGHQIRSYVLQPYQLVKDLRTGVESTNPQEVLDGAVTP
HHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHH
FMEAALAHRIQGGGEMIEDID
HHHHHHHHHHCCCCHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA