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The map label for this gene is nfdA [H]

Identifier: 225686415

GI number: 225686415

Start: 594866

End: 596695

Strand: Direct

Name: nfdA [H]

Synonym: BMEA_B0608

Alternate gene names: 225686415

Gene position: 594866-596695 (Clockwise)

Preceding gene: 225686414

Following gene: 225686419

Centisome position: 50.18

GC content: 56.17

Gene sequence:

>1830_bases
TTGACTTGTTTTAGAAAACAATATAAAATTATAATAATACTTATTAAGCCATCTCTAATATACGTAAAATTCATCGGAAG
CAAAATATTGGGGGTGTCTCCCATGTGCCTGATGTGCGGCCCTGCAGCAAAAACAATACTGAATATTACACGGCGCAACC
TTCTCAAGGGGTTGGGAGCGGCCAGCCTGACAGCGGCAATACCATTTCGCGCGGAAAGTGCGGATAGCGAGCCGGGGCAG
ATCGTCTTTTTCGGCGGGCCGATCATCACCATGGAACCCGGCGCAGAACAGGTTGAAGCCGTAGCCGTCACCAATGGTGT
TATCAGCGCAGCCGGAAGCAAGGCGGAAATACTGGACAAGAAGGCTACCGACGTGCTGCTCGTGGATTTGAAGGGGCGCA
CGCTTCTGCCCGGCTTCATCGATCCGCATATGCACACGATCTTTTCCTATTTCGAGGACTGGCTCGATATGGGCCCCTTC
GTCAACGGCTCCTACGAGCAGATCATGGACAAGCTGCGCGCCGACGCAAAAGCCGCCAAGCCCGGCGATTGGGTGCGTGG
CTGGCAGTATGATCCCTCCATCACGCCGGGTGCGCAGCCGATCACGCTGGCACTTCTTGATGAAATCGCGCCGAACAATC
CGCTTTTTATCCTCGAAGGCAACGGTCATGTCACTTACGCCAATTCAAAAGCGTTTGAATTGGCCGGTATCACAAAAGAC
ACACAAGACCCGCCCGAAGGCCGCTATATCAAGGATAAGAACGGCAATCTTTCCGGCCGCATCGAAGAATATCCGGCTTT
TGTTCCCTTTGTGGAGAAGATGCCGCAGGTTTCCCCCGATGAAATGGAGCGCCGTGTGCGTGCGCTCTTCGACAGGGGCG
CCAGCAATGGCTGCACTGCGATGATGGATTGCGGCCTCGGCCTTCTCATGGGGGAACAGGATGTGCGCACCATTCAGGGC
GTCGTGGCAAAAGATACGCCGGTACGTGTGCGCGGCGCACTCGCTTCTACCATGTATGACAAATGGGTGGAGCTTGGTTT
CAAACCGGGCTTTGGCGATGATCTCTTCCGCATTCACGCCATAAAGGCATGGGTGGATGGTTCAAATCAGGCGCGCACCG
GCTATATGCGCGAGCCTTATCTGAATTCCACCACGCGCGGCCAGTTGAACTATTCTTTGGAAGACTTGACCGCAACGATG
AAACGCTCGCATGAGGACGGCTGGCAAATCTGCGTTCACGCCAACGGGGATGCGGGCATCGACAATGCGCTGAAGGCCTA
TGCGGCCACCTTGCAGGATGCGCCCCGCGCCGATCATCGTCACCGCATCGAGCATTGCTCGGTCCTGCATGACGAGCAGA
TCAAATCCATGGGTGATCTCGGCCTCTCCCCCTCTTTCCTCATCGGCCATGTGGACTATTGGGGCGAAGCTTTCCGCGAC
GATATTCTCGGTCCCGAACGCGCCAATCTCTATGATCGTTGCGGCTCGGCCCGGAAGGCCGGCCTCCGGATCAGCCTTCA
CTCCGATTATAACGTCACCCCGATCAAGCCCTTGCAGCATATCCAGACGGCTGTCACGCGCATCATGCGCGCCAATGGTG
AAGTGCTGAACCCGGATGAGCGCATTCCGGTTGAAGCGGCAATCCGCGCCGTGACGATCGACGCGGCCTGGCAGTGCCAC
ATGGATGATCTCACCGGCAGCATCAAGGCGGGAAAGGCTGGGGATTTCGTCATTCTGGAAGACAATCCACTGACGGTTGA
CCCGGACAAGATCAGCCAGATCAAGGTTAGCGAAACCTGGCTGGACGGCAAGCGCCGCTATTCGGCCTGA

Upstream 100 bases:

>100_bases
ACAGAAAAATCGCCAATATTACCCAACTCAACAAGGAAGAATGAGTATCTATTGACACTTAAATATCTAGAACAGAAATT
GGATTTATCTATTTTAACTA

Downstream 100 bases:

>100_bases
CCGAAGCCTTCTCATGCAAAACCGGCCCAAACAAAACCCGGATGGCTCGCCATCCGGGTTTTGTTTTGGTTCATGCCGTA
TCAAGTGCTTTCAAGCACAC

Product: amidohydrolase 3

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 609; Mature: 608

Protein sequence:

>609_residues
MTCFRKQYKIIIILIKPSLIYVKFIGSKILGVSPMCLMCGPAAKTILNITRRNLLKGLGAASLTAAIPFRAESADSEPGQ
IVFFGGPIITMEPGAEQVEAVAVTNGVISAAGSKAEILDKKATDVLLVDLKGRTLLPGFIDPHMHTIFSYFEDWLDMGPF
VNGSYEQIMDKLRADAKAAKPGDWVRGWQYDPSITPGAQPITLALLDEIAPNNPLFILEGNGHVTYANSKAFELAGITKD
TQDPPEGRYIKDKNGNLSGRIEEYPAFVPFVEKMPQVSPDEMERRVRALFDRGASNGCTAMMDCGLGLLMGEQDVRTIQG
VVAKDTPVRVRGALASTMYDKWVELGFKPGFGDDLFRIHAIKAWVDGSNQARTGYMREPYLNSTTRGQLNYSLEDLTATM
KRSHEDGWQICVHANGDAGIDNALKAYAATLQDAPRADHRHRIEHCSVLHDEQIKSMGDLGLSPSFLIGHVDYWGEAFRD
DILGPERANLYDRCGSARKAGLRISLHSDYNVTPIKPLQHIQTAVTRIMRANGEVLNPDERIPVEAAIRAVTIDAAWQCH
MDDLTGSIKAGKAGDFVILEDNPLTVDPDKISQIKVSETWLDGKRRYSA

Sequences:

>Translated_609_residues
MTCFRKQYKIIIILIKPSLIYVKFIGSKILGVSPMCLMCGPAAKTILNITRRNLLKGLGAASLTAAIPFRAESADSEPGQ
IVFFGGPIITMEPGAEQVEAVAVTNGVISAAGSKAEILDKKATDVLLVDLKGRTLLPGFIDPHMHTIFSYFEDWLDMGPF
VNGSYEQIMDKLRADAKAAKPGDWVRGWQYDPSITPGAQPITLALLDEIAPNNPLFILEGNGHVTYANSKAFELAGITKD
TQDPPEGRYIKDKNGNLSGRIEEYPAFVPFVEKMPQVSPDEMERRVRALFDRGASNGCTAMMDCGLGLLMGEQDVRTIQG
VVAKDTPVRVRGALASTMYDKWVELGFKPGFGDDLFRIHAIKAWVDGSNQARTGYMREPYLNSTTRGQLNYSLEDLTATM
KRSHEDGWQICVHANGDAGIDNALKAYAATLQDAPRADHRHRIEHCSVLHDEQIKSMGDLGLSPSFLIGHVDYWGEAFRD
DILGPERANLYDRCGSARKAGLRISLHSDYNVTPIKPLQHIQTAVTRIMRANGEVLNPDERIPVEAAIRAVTIDAAWQCH
MDDLTGSIKAGKAGDFVILEDNPLTVDPDKISQIKVSETWLDGKRRYSA
>Mature_608_residues
TCFRKQYKIIIILIKPSLIYVKFIGSKILGVSPMCLMCGPAAKTILNITRRNLLKGLGAASLTAAIPFRAESADSEPGQI
VFFGGPIITMEPGAEQVEAVAVTNGVISAAGSKAEILDKKATDVLLVDLKGRTLLPGFIDPHMHTIFSYFEDWLDMGPFV
NGSYEQIMDKLRADAKAAKPGDWVRGWQYDPSITPGAQPITLALLDEIAPNNPLFILEGNGHVTYANSKAFELAGITKDT
QDPPEGRYIKDKNGNLSGRIEEYPAFVPFVEKMPQVSPDEMERRVRALFDRGASNGCTAMMDCGLGLLMGEQDVRTIQGV
VAKDTPVRVRGALASTMYDKWVELGFKPGFGDDLFRIHAIKAWVDGSNQARTGYMREPYLNSTTRGQLNYSLEDLTATMK
RSHEDGWQICVHANGDAGIDNALKAYAATLQDAPRADHRHRIEHCSVLHDEQIKSMGDLGLSPSFLIGHVDYWGEAFRDD
ILGPERANLYDRCGSARKAGLRISLHSDYNVTPIKPLQHIQTAVTRIMRANGEVLNPDERIPVEAAIRAVTIDAAWQCHM
DDLTGSIKAGKAGDFVILEDNPLTVDPDKISQIKVSETWLDGKRRYSA

Specific function: Hydrolyzes N-substituted formamides, but not amides. N- benzylformamide is the preferred substrate, while N-butylformamide is hydrolyzed at a much lower rate. Has very low activity towards allylformamide, N-(2-cyclohex-1-enylethyl)formamide and N-(alpha-

COG id: COG1574

COG function: function code R; Predicted metal-dependent hydrolase with the TIM-barrel fold

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013108
- InterPro:   IPR011059 [H]

Pfam domain/function: PF07969 Amidohydro_3 [H]

EC number: =3.5.1.91 [H]

Molecular weight: Translated: 67017; Mature: 66886

Theoretical pI: Translated: 6.29; Mature: 6.29

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTCFRKQYKIIIILIKPSLIYVKFIGSKILGVSPMCLMCGPAAKTILNITRRNLLKGLGA
CCCCCCCCEEEEEEECCCEEEEEECCCHHCCCCCHHEEECHHHHHHHHHHHHHHHHHCCC
ASLTAAIPFRAESADSEPGQIVFFGGPIITMEPGAEQVEAVAVTNGVISAAGSKAEILDK
HHHHEECCEECCCCCCCCCEEEEECCEEEEECCCHHHHEEEEEECCHHCCCCCHHHHHCC
KATDVLLVDLKGRTLLPGFIDPHMHTIFSYFEDWLDMGPFVNGSYEQIMDKLRADAKAAK
CCCCEEEEEECCCEECCCCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCC
PGDWVRGWQYDPSITPGAQPITLALLDEIAPNNPLFILEGNGHVTYANSKAFELAGITKD
CCCCCCCCCCCCCCCCCCCCEEHEEHHHHCCCCCEEEEECCCEEEEECCCEEEEECCCCC
TQDPPEGRYIKDKNGNLSGRIEEYPAFVPFVEKMPQVSPDEMERRVRALFDRGASNGCTA
CCCCCCCCEEECCCCCCCCCHHHCCCCHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCHH
MMDCGLGLLMGEQDVRTIQGVVAKDTPVRVRGALASTMYDKWVELGFKPGFGDDLFRIHA
HHHCCCEEEECCHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHCCCCCCCCCCCEEEEE
IKAWVDGSNQARTGYMREPYLNSTTRGQLNYSLEDLTATMKRSHEDGWQICVHANGDAGI
EEEEECCCCCCCCCCCCCCCCCCCCCCEEEECHHHHHHHHHHCCCCCEEEEEECCCCCCH
DNALKAYAATLQDAPRADHRHRIEHCSVLHDEQIKSMGDLGLSPSFLIGHVDYWGEAFRD
HHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEECHHHHHHHHHH
DILGPERANLYDRCGSARKAGLRISLHSDYNVTPIKPLQHIQTAVTRIMRANGEVLNPDE
HCCCCCCCCHHHHCCCCHHCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHCCCCEECCCC
RIPVEAAIRAVTIDAAWQCHMDDLTGSIKAGKAGDFVILEDNPLTVDPDKISQIKVSETW
CCCHHHHEEEEEEECCEEEEHHHCCCCCCCCCCCCEEEECCCCCEECCCCCCEEEEEHHH
LDGKRRYSA
CCCCCCCCC
>Mature Secondary Structure 
TCFRKQYKIIIILIKPSLIYVKFIGSKILGVSPMCLMCGPAAKTILNITRRNLLKGLGA
CCCCCCCEEEEEEECCCEEEEEECCCHHCCCCCHHEEECHHHHHHHHHHHHHHHHHCCC
ASLTAAIPFRAESADSEPGQIVFFGGPIITMEPGAEQVEAVAVTNGVISAAGSKAEILDK
HHHHEECCEECCCCCCCCCEEEEECCEEEEECCCHHHHEEEEEECCHHCCCCCHHHHHCC
KATDVLLVDLKGRTLLPGFIDPHMHTIFSYFEDWLDMGPFVNGSYEQIMDKLRADAKAAK
CCCCEEEEEECCCEECCCCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCC
PGDWVRGWQYDPSITPGAQPITLALLDEIAPNNPLFILEGNGHVTYANSKAFELAGITKD
CCCCCCCCCCCCCCCCCCCCEEHEEHHHHCCCCCEEEEECCCEEEEECCCEEEEECCCCC
TQDPPEGRYIKDKNGNLSGRIEEYPAFVPFVEKMPQVSPDEMERRVRALFDRGASNGCTA
CCCCCCCCEEECCCCCCCCCHHHCCCCHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCHH
MMDCGLGLLMGEQDVRTIQGVVAKDTPVRVRGALASTMYDKWVELGFKPGFGDDLFRIHA
HHHCCCEEEECCHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHCCCCCCCCCCCEEEEE
IKAWVDGSNQARTGYMREPYLNSTTRGQLNYSLEDLTATMKRSHEDGWQICVHANGDAGI
EEEEECCCCCCCCCCCCCCCCCCCCCCEEEECHHHHHHHHHHCCCCCEEEEEECCCCCCH
DNALKAYAATLQDAPRADHRHRIEHCSVLHDEQIKSMGDLGLSPSFLIGHVDYWGEAFRD
HHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEECHHHHHHHHHH
DILGPERANLYDRCGSARKAGLRISLHSDYNVTPIKPLQHIQTAVTRIMRANGEVLNPDE
HCCCCCCCCHHHHCCCCHHCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHCCCCEECCCC
RIPVEAAIRAVTIDAAWQCHMDDLTGSIKAGKAGDFVILEDNPLTVDPDKISQIKVSETW
CCCHHHHEEEEEEECCEEEEHHHCCCCCCCCCCCCEEEECCCCCEECCCCCCEEEEEHHH
LDGKRRYSA
CCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA