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The map label for this gene is nrdE [H]

Identifier: 225686157

GI number: 225686157

Start: 298238

End: 300379

Strand: Direct

Name: nrdE [H]

Synonym: BMEA_B0316

Alternate gene names: 225686157

Gene position: 298238-300379 (Clockwise)

Preceding gene: 225686156

Following gene: 225686158

Centisome position: 25.16

GC content: 53.5

Gene sequence:

>2142_bases
ATGACCGCCGGAAAGCCTGCCAAGTCTTCGGACGGGCTGGACTACCATGCGCTCAACGCCATGCTGAATCTCTATGATGA
GGACGGTAAAATTCAGCTCGACTGCGACCGCCAGGCCGCACGGCAATATTTCCTCCAGCATGTGAACCAGAATACGGTCT
TCTTCCATAACCTCAGGGAGAAGCTCGATTATCTGGTGGCGGAAGGCTATTACGAACAGACGGTGCTGGATCAATATTCC
TTCAATTTCGTGCGCGATCTTTTCGATGAAGCCTATGCGAGGAAATTCCGCTTTCCGACCTTTCTCGGCGCCTTCAAATA
TTACACCAGCTACACGCTGAAAACCTTCGATGGAAAGCGTTATCTGGAGCGTTATGAGGATCGTGTCTGCATGGTGGCGC
TGACGCTTGCGCGCGGTGACGAGCAGCTTGCGCGCGACCTTGTGGAAGAGATTATTTCAGGGCGCTACCAGCCCGCGACG
CCCACTTTCCTCAATGCCGGCAAGAAGCAGCGCGGCGAACTGGTATCCTGTTTTCTGCTGCGTGTTGAAGACAATATGGA
ATCCATCGGACGGTCCATCAATTCCGCGCTGCAATTGTCGAAGCGCGGTGGCGGCGTGGCGCTCTGCCTCACCAATCTTC
GGGAAGCCGGTGCGCCGATCAAGCAGATCGAGAACCAGTCTTCCGGCATTATTCCGGTGATGAAATTGCTGGAGGATTCC
TTTTCCTATGCCAACCAGCTTGGCGCACGGCAGGGGGCTGGCGCGGTCTACCTCCATGCGCATCACCCGGATATCATGCG
TTTTCTCGATACCAAACGCGAAAATGCGGATGAAAAGATCCGCATCAAGACCCTTTCACTTGGCGTTGTCATTCCCGACA
TTACTTTCGAGCTCGCCCGCAACAACGAGGATATGTATCTCTTTTCACCTTACGATGTGCAGCGGGTTTACGGTGTTCCG
CTGACCGAAATTTCGGTGACGGAAAAGTATCGTGAGATGGTCAATGATGCGCAGATTGCCAAAAAGAAGATCAATGCGCG
CGAATTCTTTCAGGTGCTGGCCGAAATTCAGTTTGAATCCGGTTATCCTTACATCATGTTCGAGGATACGGTGAACCGCG
CCAACCCGATCGATGGGCGCATCACCATGAGCAATCTCTGTTCGGAAATTCTTCAGGTGAGCGAGGCGAGCCTCTTCCAT
GAAGACCTTTCCTATGAGTATCTCGGCAAGGATATTTCGTGCAATCTGGGTTCGCTGAACATTGCCGCCGCGATGGATGC
GCCTGATTTCGGCAAGACCATTGAAACATCCATTCGCGCCCTGACGGCTGTCAGCGACATGAGCCATATTGGTTGCGTGC
CATCGGTTGCGCGCGGCAATGCTGAAAGCCACGCCATTGGCCTTGGCCAGATGAACCTGCATGGCTATCTTGCCCGCGAG
CGGATTTTCTACGGTTCCGAAGAAGGCGTCGATTTCACGAATATCTATTTCTATACCGTGGCTTACCATGCGGTGCGCGC
TTCAAATCGCATTGCGGTCGAAACCGGAAAATCCTTCAAGGGATTTGAAAAATCCAAATATGCCTCGGGTGAATATTTCG
ACAAATATACAGATCAGGCATGGGCTCCGGCCACGGAAAAGGTGCGCCGGATATTCGAGGATGCCGGCATTGCGATCCCG
ACCCAGGATGACTGGTGCGCGTTGAAAAAGGCTGTGATGGAAGGCGGGCTTTATAACCAGAACCTTCAGGCTGTGCCGCC
GACCGGATCGATCTCCTACATCAATCATTCGACCTCCTCAATCCACCCCATCGTGTCGAAGATCGAAATCCGCAAGGAGG
GCAAGATCGGCCGTGTCTATTACCCGGCGGCCTTCATGACCAACGATAATCTTGAATATTATCAGGATGCCTATGAGATT
GGGCCGGAGAAGATCATCGACACCTATGCGGCGGCAACCCAGCATGTCGATCAGGGTCTGTCGCTGACCCTGTTCTTCCG
CGATACCGCAACCACCCGCGATATCAACCGCGCCCAGATCTATGCGTGGAAAAAAGGCATCAAGACCATCTACTACATTC
GCCTGCGCCAGATGGCGCTTGAAGGAACGCAGGTGCAAGGCTGCGTTTCCTGCGCGCTGTGA

Upstream 100 bases:

>100_bases
ATGTCAGAAACGGGATGGAACAATTTTGGACACGCCAGACACAAGCCTGATCCGGGAACGCGAGGCAGAGGCGGTGCAGG
GCGCACCGGAGCCGGTGGCA

Downstream 100 bases:

>100_bases
CCGAGGAAAGTTTCATGAATATGCAACATAAAACCGCGACCAAAGGCAGGCCCGCTTCCGTGCGCGCCATCAACTGGAAC
CGCATCGAGGATGATAAGGA

Product: ribonucleotide-diphosphate reductase subunit alpha

Products: NA

Alternate protein names: Ribonucleotide reductase R1 subunit [H]

Number of amino acids: Translated: 713; Mature: 712

Protein sequence:

>713_residues
MTAGKPAKSSDGLDYHALNAMLNLYDEDGKIQLDCDRQAARQYFLQHVNQNTVFFHNLREKLDYLVAEGYYEQTVLDQYS
FNFVRDLFDEAYARKFRFPTFLGAFKYYTSYTLKTFDGKRYLERYEDRVCMVALTLARGDEQLARDLVEEIISGRYQPAT
PTFLNAGKKQRGELVSCFLLRVEDNMESIGRSINSALQLSKRGGGVALCLTNLREAGAPIKQIENQSSGIIPVMKLLEDS
FSYANQLGARQGAGAVYLHAHHPDIMRFLDTKRENADEKIRIKTLSLGVVIPDITFELARNNEDMYLFSPYDVQRVYGVP
LTEISVTEKYREMVNDAQIAKKKINAREFFQVLAEIQFESGYPYIMFEDTVNRANPIDGRITMSNLCSEILQVSEASLFH
EDLSYEYLGKDISCNLGSLNIAAAMDAPDFGKTIETSIRALTAVSDMSHIGCVPSVARGNAESHAIGLGQMNLHGYLARE
RIFYGSEEGVDFTNIYFYTVAYHAVRASNRIAVETGKSFKGFEKSKYASGEYFDKYTDQAWAPATEKVRRIFEDAGIAIP
TQDDWCALKKAVMEGGLYNQNLQAVPPTGSISYINHSTSSIHPIVSKIEIRKEGKIGRVYYPAAFMTNDNLEYYQDAYEI
GPEKIIDTYAAATQHVDQGLSLTLFFRDTATTRDINRAQIYAWKKGIKTIYYIRLRQMALEGTQVQGCVSCAL

Sequences:

>Translated_713_residues
MTAGKPAKSSDGLDYHALNAMLNLYDEDGKIQLDCDRQAARQYFLQHVNQNTVFFHNLREKLDYLVAEGYYEQTVLDQYS
FNFVRDLFDEAYARKFRFPTFLGAFKYYTSYTLKTFDGKRYLERYEDRVCMVALTLARGDEQLARDLVEEIISGRYQPAT
PTFLNAGKKQRGELVSCFLLRVEDNMESIGRSINSALQLSKRGGGVALCLTNLREAGAPIKQIENQSSGIIPVMKLLEDS
FSYANQLGARQGAGAVYLHAHHPDIMRFLDTKRENADEKIRIKTLSLGVVIPDITFELARNNEDMYLFSPYDVQRVYGVP
LTEISVTEKYREMVNDAQIAKKKINAREFFQVLAEIQFESGYPYIMFEDTVNRANPIDGRITMSNLCSEILQVSEASLFH
EDLSYEYLGKDISCNLGSLNIAAAMDAPDFGKTIETSIRALTAVSDMSHIGCVPSVARGNAESHAIGLGQMNLHGYLARE
RIFYGSEEGVDFTNIYFYTVAYHAVRASNRIAVETGKSFKGFEKSKYASGEYFDKYTDQAWAPATEKVRRIFEDAGIAIP
TQDDWCALKKAVMEGGLYNQNLQAVPPTGSISYINHSTSSIHPIVSKIEIRKEGKIGRVYYPAAFMTNDNLEYYQDAYEI
GPEKIIDTYAAATQHVDQGLSLTLFFRDTATTRDINRAQIYAWKKGIKTIYYIRLRQMALEGTQVQGCVSCAL
>Mature_712_residues
TAGKPAKSSDGLDYHALNAMLNLYDEDGKIQLDCDRQAARQYFLQHVNQNTVFFHNLREKLDYLVAEGYYEQTVLDQYSF
NFVRDLFDEAYARKFRFPTFLGAFKYYTSYTLKTFDGKRYLERYEDRVCMVALTLARGDEQLARDLVEEIISGRYQPATP
TFLNAGKKQRGELVSCFLLRVEDNMESIGRSINSALQLSKRGGGVALCLTNLREAGAPIKQIENQSSGIIPVMKLLEDSF
SYANQLGARQGAGAVYLHAHHPDIMRFLDTKRENADEKIRIKTLSLGVVIPDITFELARNNEDMYLFSPYDVQRVYGVPL
TEISVTEKYREMVNDAQIAKKKINAREFFQVLAEIQFESGYPYIMFEDTVNRANPIDGRITMSNLCSEILQVSEASLFHE
DLSYEYLGKDISCNLGSLNIAAAMDAPDFGKTIETSIRALTAVSDMSHIGCVPSVARGNAESHAIGLGQMNLHGYLARER
IFYGSEEGVDFTNIYFYTVAYHAVRASNRIAVETGKSFKGFEKSKYASGEYFDKYTDQAWAPATEKVRRIFEDAGIAIPT
QDDWCALKKAVMEGGLYNQNLQAVPPTGSISYINHSTSSIHPIVSKIEIRKEGKIGRVYYPAAFMTNDNLEYYQDAYEIG
PEKIIDTYAAATQHVDQGLSLTLFFRDTATTRDINRAQIYAWKKGIKTIYYIRLRQMALEGTQVQGCVSCAL

Specific function: Provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides [H]

COG id: COG0209

COG function: function code F; Ribonucleotide reductase, alpha subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ribonucleoside diphosphate reductase large chain family [H]

Homologues:

Organism=Homo sapiens, GI4506749, Length=657, Percent_Identity=26.027397260274, Blast_Score=177, Evalue=3e-44,
Organism=Escherichia coli, GI1789030, Length=705, Percent_Identity=71.7730496453901, Blast_Score=1081, Evalue=0.0,
Organism=Escherichia coli, GI2367133, Length=583, Percent_Identity=25.0428816466552, Blast_Score=159, Evalue=5e-40,
Organism=Caenorhabditis elegans, GI17555242, Length=653, Percent_Identity=26.0336906584992, Blast_Score=174, Evalue=2e-43,
Organism=Saccharomyces cerevisiae, GI6322123, Length=715, Percent_Identity=26.4335664335664, Blast_Score=173, Evalue=7e-44,
Organism=Saccharomyces cerevisiae, GI6320914, Length=655, Percent_Identity=26.1068702290076, Blast_Score=171, Evalue=5e-43,
Organism=Drosophila melanogaster, GI17352481, Length=656, Percent_Identity=25.9146341463415, Blast_Score=162, Evalue=5e-40,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013346
- InterPro:   IPR013509
- InterPro:   IPR000788
- InterPro:   IPR008926
- InterPro:   IPR013554 [H]

Pfam domain/function: PF02867 Ribonuc_red_lgC; PF00317 Ribonuc_red_lgN; PF08343 RNR_N [H]

EC number: =1.17.4.1 [H]

Molecular weight: Translated: 80573; Mature: 80442

Theoretical pI: Translated: 6.06; Mature: 6.06

Prosite motif: PS00089 RIBORED_LARGE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAGKPAKSSDGLDYHALNAMLNLYDEDGKIQLDCDRQAARQYFLQHVNQNTVFFHNLRE
CCCCCCCCCCCCCCHHHHHHHHHHCCCCCCEEEECCHHHHHHHHHHHCCCCEEEHHHHHH
KLDYLVAEGYYEQTVLDQYSFNFVRDLFDEAYARKFRFPTFLGAFKYYTSYTLKTFDGKR
HHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHEEEEECCCHH
YLERYEDRVCMVALTLARGDEQLARDLVEEIISGRYQPATPTFLNAGKKQRGELVSCFLL
HHHHHHCCEEEEEEEHHCCHHHHHHHHHHHHHCCCCCCCCCHHHCCCCHHHCCCEEHHEE
RVEDNMESIGRSINSALQLSKRGGGVALCLTNLREAGAPIKQIENQSSGIIPVMKLLEDS
HHHHHHHHHHHHHHHHHHHHCCCCCEEEEEHHHHHHCCCHHHHCCCCCCHHHHHHHHHHH
FSYANQLGARQGAGAVYLHAHHPDIMRFLDTKRENADEKIRIKTLSLGVVIPDITFELAR
HHHHHHHCCCCCCCEEEEEECCHHHHHHHHHHCCCCCCEEEEEEEEEEEEECCCEEEEEC
NNEDMYLFSPYDVQRVYGVPLTEISVTEKYREMVNDAQIAKKKINAREFFQVLAEIQFES
CCCCEEEECCCCCHHHCCCCCCEEHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCC
GYPYIMFEDTVNRANPIDGRITMSNLCSEILQVSEASLFHEDLSYEYLGKDISCNLGSLN
CCCEEEEECCCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHCCHHHCCCCCCCCCCCEE
IAAAMDAPDFGKTIETSIRALTAVSDMSHIGCVPSVARGNAESHAIGLGQMNLHGYLARE
EEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHCCCCCHHEEEECCCCHHHHHHHH
RIFYGSEEGVDFTNIYFYTVAYHAVRASNRIAVETGKSFKGFEKSKYASGEYFDKYTDQA
HEEECCCCCCCHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCCCCCHHHHHCCCC
WAPATEKVRRIFEDAGIAIPTQDDWCALKKAVMEGGLYNQNLQAVPPTGSISYINHSTSS
CCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCCCCCEECCCCCCEEEEECCCCH
IHPIVSKIEIRKEGKIGRVYYPAAFMTNDNLEYYQDAYEIGPEKIIDTYAAATQHVDQGL
HHHHHHHHHHHCCCCCCEEEECEEEEECCCHHHHHHHHHCCHHHHHHHHHHHHHHHCCCC
SLTLFFRDTATTRDINRAQIYAWKKGIKTIYYIRLRQMALEGTQVQGCVSCAL
EEEEEEECCCCCCCCCHHHHHHHHHCCCEEEHHHHHHHHHCCCCHHHHHHHCC
>Mature Secondary Structure 
TAGKPAKSSDGLDYHALNAMLNLYDEDGKIQLDCDRQAARQYFLQHVNQNTVFFHNLRE
CCCCCCCCCCCCCHHHHHHHHHHCCCCCCEEEECCHHHHHHHHHHHCCCCEEEHHHHHH
KLDYLVAEGYYEQTVLDQYSFNFVRDLFDEAYARKFRFPTFLGAFKYYTSYTLKTFDGKR
HHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHEEEEECCCHH
YLERYEDRVCMVALTLARGDEQLARDLVEEIISGRYQPATPTFLNAGKKQRGELVSCFLL
HHHHHHCCEEEEEEEHHCCHHHHHHHHHHHHHCCCCCCCCCHHHCCCCHHHCCCEEHHEE
RVEDNMESIGRSINSALQLSKRGGGVALCLTNLREAGAPIKQIENQSSGIIPVMKLLEDS
HHHHHHHHHHHHHHHHHHHHCCCCCEEEEEHHHHHHCCCHHHHCCCCCCHHHHHHHHHHH
FSYANQLGARQGAGAVYLHAHHPDIMRFLDTKRENADEKIRIKTLSLGVVIPDITFELAR
HHHHHHHCCCCCCCEEEEEECCHHHHHHHHHHCCCCCCEEEEEEEEEEEEECCCEEEEEC
NNEDMYLFSPYDVQRVYGVPLTEISVTEKYREMVNDAQIAKKKINAREFFQVLAEIQFES
CCCCEEEECCCCCHHHCCCCCCEEHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCC
GYPYIMFEDTVNRANPIDGRITMSNLCSEILQVSEASLFHEDLSYEYLGKDISCNLGSLN
CCCEEEEECCCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHCCHHHCCCCCCCCCCCEE
IAAAMDAPDFGKTIETSIRALTAVSDMSHIGCVPSVARGNAESHAIGLGQMNLHGYLARE
EEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHCCCCCHHEEEECCCCHHHHHHHH
RIFYGSEEGVDFTNIYFYTVAYHAVRASNRIAVETGKSFKGFEKSKYASGEYFDKYTDQA
HEEECCCCCCCHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCCCCCHHHHHCCCC
WAPATEKVRRIFEDAGIAIPTQDDWCALKKAVMEGGLYNQNLQAVPPTGSISYINHSTSS
CCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCCCCCEECCCCCCEEEEECCCCH
IHPIVSKIEIRKEGKIGRVYYPAAFMTNDNLEYYQDAYEIGPEKIIDTYAAATQHVDQGL
HHHHHHHHHHHCCCCCCEEEECEEEEECCCHHHHHHHHHCCHHHHHHHHHHHHHHHCCCC
SLTLFFRDTATTRDINRAQIYAWKKGIKTIYYIRLRQMALEGTQVQGCVSCAL
EEEEEEECCCCCCCCCHHHHHHHHHCCCEEEHHHHHHHHHCCCCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]