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The map label for this gene is aceF [H]

Identifier: 225630846

GI number: 225630846

Start: 1241806

End: 1243170

Strand: Direct

Name: aceF [H]

Synonym: WRi_011520

Alternate gene names: 225630846

Gene position: 1241806-1243170 (Clockwise)

Preceding gene: 225630844

Following gene: 225630847

Centisome position: 85.89

GC content: 36.7

Gene sequence:

>1365_bases
ATGCCTATAGAAATATTAATGCCTGCTCTTTCACCGACAATGAGCAAAACTGGAGGAAAAATTGTAAAGTGGCATAAAAA
AGAACAAGACAAAGTTGAAGTAGGCGACGTAATTGCTGAGATAGAGACTGATAAAGCCATAATGGAGTTTGAATCTATAG
ATGAAGGAGTTTTAGCAAAAATTTTAGTAACAGAAGGAACAAGTGGCGTGCCTGTAAATCAACCAATAGCTTTAATGCTG
GAAGAAGGAGAAGATGAAAGTGCACTTAACAACTATACTTCAACTTCCATCAATAGTGCAGTTAAAAAGGAAGTGACAAA
AAGTGCTGTTGATAATCAAAAATCAGAACACCAAGATTTAAACGGAAAGCCAATAAGTCATTCTTCGGTGTCATTCCAGC
GCGTGACGCTGGAATCCAACCCCTCAATGTCATCCCAGTGCTTGACACTGGGATCTAGAAAAGAAGAATGGATCCCAGTG
TCAGCTACTCAGATGACAGAGGGTTATGCAGAAGGTAGAACAAAAATAAGCCCATTAGCTAAGAAAATAGCTCAAAATGA
AGGGGTTAATGTGCAGCAATTAAAAGGTACAGGACCATATGGTCGCATAATTAAGGCTGATGTGTTGGAGTTTTTAGGCA
GTGGTATACATACTGAAAGTCCTGAGAAAGATACCATAGTTGAAGTAAGCAACATGCGCCAAGTGATAGCACAGCGCCTG
ACTGAATCAAAACAGAATGTTCCACATTTTTATTTAACTGTAGATTGCCAGGTTGATAAGTTAATATCGTTAAAAAACGA
GATTAACTCAGCAGATGAAAACAATAAAGTAACAATTAATGATCTAATCATAAAAGCTGCAGCTTTCAGCATGAAAAAAT
TTCCTGATATAAATTCTTCGTGGATAGATAATAAAATACTGAGATATTCAAATATAGATATTTCAATCGCTGTGGCACTT
GAAGATGGGCTAATTACTCCTATAGTAAAAAATGCTGATAAAAAGGGTATTTTATCTATATCAAAAGAAGTGAAAGATTT
AGTAAGCAGAGCAAGATCTGGAAAATTGAAACCTGAAGAGTTTCAAGGAGGAGGGTTTACTATCTCCAACTTAGGGATGT
TTGGTATAAAAGCCTTCAGTGCTATAATCAATCCGCCGCAATCTTGCATTATGGCAGTTGGTGCATCTAAAAAACAACCA
ATTGTTATGAATGAGAAAATAGAAATAGCAGAAATAATGACAGTAACGCTCTCTGTTGACCATAGAGCAGTTGATGGGGC
ACTCGGAGCAAAGTTTTTAAATGCCTTTAAGCATTATATAGAGAATCCTCTGGTGATGCTTATTGAAACTGCGGTAATCT
ATTAG

Upstream 100 bases:

>100_bases
GAGGGATAGTGTAACAGACTTATTCATCATAATTCACGATTAGGTTGACATAAGATAAAGAGTAAATTAAAAATTGAATA
GATCTTTAAATGCAAAATCA

Downstream 100 bases:

>100_bases
CATTGTTATTACAAAATTAATACCTCTAAGTTTATACTTTTATTAGTTAATAGGTTTAGAGGTAAAAAAATGAATACTAC
AGTAGAAAAAAATAATCCAA

Product: branched-chain alpha-keto acid dehydrogenase subunit E2

Products: NA

Alternate protein names: Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex; E2 [H]

Number of amino acids: Translated: 454; Mature: 453

Protein sequence:

>454_residues
MPIEILMPALSPTMSKTGGKIVKWHKKEQDKVEVGDVIAEIETDKAIMEFESIDEGVLAKILVTEGTSGVPVNQPIALML
EEGEDESALNNYTSTSINSAVKKEVTKSAVDNQKSEHQDLNGKPISHSSVSFQRVTLESNPSMSSQCLTLGSRKEEWIPV
SATQMTEGYAEGRTKISPLAKKIAQNEGVNVQQLKGTGPYGRIIKADVLEFLGSGIHTESPEKDTIVEVSNMRQVIAQRL
TESKQNVPHFYLTVDCQVDKLISLKNEINSADENNKVTINDLIIKAAAFSMKKFPDINSSWIDNKILRYSNIDISIAVAL
EDGLITPIVKNADKKGILSISKEVKDLVSRARSGKLKPEEFQGGGFTISNLGMFGIKAFSAIINPPQSCIMAVGASKKQP
IVMNEKIEIAEIMTVTLSVDHRAVDGALGAKFLNAFKHYIENPLVMLIETAVIY

Sequences:

>Translated_454_residues
MPIEILMPALSPTMSKTGGKIVKWHKKEQDKVEVGDVIAEIETDKAIMEFESIDEGVLAKILVTEGTSGVPVNQPIALML
EEGEDESALNNYTSTSINSAVKKEVTKSAVDNQKSEHQDLNGKPISHSSVSFQRVTLESNPSMSSQCLTLGSRKEEWIPV
SATQMTEGYAEGRTKISPLAKKIAQNEGVNVQQLKGTGPYGRIIKADVLEFLGSGIHTESPEKDTIVEVSNMRQVIAQRL
TESKQNVPHFYLTVDCQVDKLISLKNEINSADENNKVTINDLIIKAAAFSMKKFPDINSSWIDNKILRYSNIDISIAVAL
EDGLITPIVKNADKKGILSISKEVKDLVSRARSGKLKPEEFQGGGFTISNLGMFGIKAFSAIINPPQSCIMAVGASKKQP
IVMNEKIEIAEIMTVTLSVDHRAVDGALGAKFLNAFKHYIENPLVMLIETAVIY
>Mature_453_residues
PIEILMPALSPTMSKTGGKIVKWHKKEQDKVEVGDVIAEIETDKAIMEFESIDEGVLAKILVTEGTSGVPVNQPIALMLE
EGEDESALNNYTSTSINSAVKKEVTKSAVDNQKSEHQDLNGKPISHSSVSFQRVTLESNPSMSSQCLTLGSRKEEWIPVS
ATQMTEGYAEGRTKISPLAKKIAQNEGVNVQQLKGTGPYGRIIKADVLEFLGSGIHTESPEKDTIVEVSNMRQVIAQRLT
ESKQNVPHFYLTVDCQVDKLISLKNEINSADENNKVTINDLIIKAAAFSMKKFPDINSSWIDNKILRYSNIDISIAVALE
DGLITPIVKNADKKGILSISKEVKDLVSRARSGKLKPEEFQGGGFTISNLGMFGIKAFSAIINPPQSCIMAVGASKKQPI
VMNEKIEIAEIMTVTLSVDHRAVDGALGAKFLNAFKHYIENPLVMLIETAVIY

Specific function: The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2). It contains multiple copies of three enzymatic components:pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydroge

COG id: COG0508

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide acyltransferase (E2) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain [H]

Homologues:

Organism=Homo sapiens, GI31711992, Length=464, Percent_Identity=42.4568965517241, Blast_Score=350, Evalue=2e-96,
Organism=Homo sapiens, GI203098816, Length=324, Percent_Identity=39.1975308641975, Blast_Score=223, Evalue=4e-58,
Organism=Homo sapiens, GI203098753, Length=324, Percent_Identity=39.1975308641975, Blast_Score=222, Evalue=6e-58,
Organism=Homo sapiens, GI260898739, Length=161, Percent_Identity=48.4472049689441, Blast_Score=155, Evalue=6e-38,
Organism=Homo sapiens, GI19923748, Length=243, Percent_Identity=32.9218106995885, Blast_Score=134, Evalue=2e-31,
Organism=Homo sapiens, GI110671329, Length=327, Percent_Identity=28.4403669724771, Blast_Score=125, Evalue=7e-29,
Organism=Escherichia coli, GI1786946, Length=291, Percent_Identity=28.5223367697594, Blast_Score=155, Evalue=4e-39,
Organism=Escherichia coli, GI1786305, Length=295, Percent_Identity=28.135593220339, Blast_Score=135, Evalue=6e-33,
Organism=Caenorhabditis elegans, GI17560088, Length=460, Percent_Identity=39.7826086956522, Blast_Score=313, Evalue=2e-85,
Organism=Caenorhabditis elegans, GI17538894, Length=312, Percent_Identity=35.2564102564103, Blast_Score=197, Evalue=8e-51,
Organism=Caenorhabditis elegans, GI17537937, Length=310, Percent_Identity=28.7096774193548, Blast_Score=142, Evalue=3e-34,
Organism=Caenorhabditis elegans, GI25146366, Length=442, Percent_Identity=27.3755656108597, Blast_Score=135, Evalue=4e-32,
Organism=Saccharomyces cerevisiae, GI6324258, Length=478, Percent_Identity=37.0292887029289, Blast_Score=291, Evalue=2e-79,
Organism=Saccharomyces cerevisiae, GI6320352, Length=241, Percent_Identity=34.0248962655602, Blast_Score=136, Evalue=6e-33,
Organism=Saccharomyces cerevisiae, GI6321632, Length=210, Percent_Identity=29.0476190476191, Blast_Score=78, Evalue=2e-15,
Organism=Drosophila melanogaster, GI20129315, Length=291, Percent_Identity=43.9862542955326, Blast_Score=259, Evalue=3e-69,
Organism=Drosophila melanogaster, GI24582497, Length=291, Percent_Identity=43.9862542955326, Blast_Score=258, Evalue=5e-69,
Organism=Drosophila melanogaster, GI18859875, Length=310, Percent_Identity=31.6129032258064, Blast_Score=157, Evalue=1e-38,
Organism=Drosophila melanogaster, GI24645909, Length=226, Percent_Identity=30.0884955752212, Blast_Score=111, Evalue=8e-25,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 3096 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR001078
- InterPro:   IPR006257
- InterPro:   IPR000089
- InterPro:   IPR023213
- InterPro:   IPR004167
- InterPro:   IPR011053 [H]

Pfam domain/function: PF00198 2-oxoacid_dh; PF00364 Biotin_lipoyl; PF02817 E3_binding [H]

EC number: =2.3.1.12 [H]

Molecular weight: Translated: 49644; Mature: 49512

Theoretical pI: Translated: 5.76; Mature: 5.76

Prosite motif: PS50968 BIOTINYL_LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPIEILMPALSPTMSKTGGKIVKWHKKEQDKVEVGDVIAEIETDKAIMEFESIDEGVLAK
CCCEEEEHHCCCHHHHCCCCEEEECCCCCCHHHHHHHHHHHHCCHHHHHHHHCCCCCEEE
ILVTEGTSGVPVNQPIALMLEEGEDESALNNYTSTSINSAVKKEVTKSAVDNQKSEHQDL
EEEECCCCCCCCCCCEEEEEECCCCHHHHHCCCHHHHHHHHHHHHHHHHHHCCCHHHCCC
NGKPISHSSVSFQRVTLESNPSMSSQCLTLGSRKEEWIPVSATQMTEGYAEGRTKISPLA
CCCCCCCCCCEEEEEEECCCCCCCHHHHHCCCCCCCCCCCCHHHHHHHHHCCCCHHHHHH
KKIAQNEGVNVQQLKGTGPYGRIIKADVLEFLGSGIHTESPEKDTIVEVSNMRQVIAQRL
HHHHHCCCCCEEEECCCCCCCHHHHHHHHHHHCCCCCCCCCCCCHHEEHHHHHHHHHHHH
TESKQNVPHFYLTVDCQVDKLISLKNEINSADENNKVTINDLIIKAAAFSMKKFPDINSS
HHHHCCCCEEEEEEECCHHHHHHHHHHHCCCCCCCCEEHHHHHHHHHHHHHHHCCCCCCC
WIDNKILRYSNIDISIAVALEDGLITPIVKNADKKGILSISKEVKDLVSRARSGKLKPEE
HHCCCCEEEECCCEEEEEEECCCCCCHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCCHH
FQGGGFTISNLGMFGIKAFSAIINPPQSCIMAVGASKKQPIVMNEKIEIAEIMTVTLSVD
CCCCCEEEECCCHHHHHHHHHHHCCHHHHHHHHCCCCCCCEEECCCEEEEEEEEEEEEEC
HRAVDGALGAKFLNAFKHYIENPLVMLIETAVIY
CHHHHHHHHHHHHHHHHHHHCCCCEEEHHHHHCC
>Mature Secondary Structure 
PIEILMPALSPTMSKTGGKIVKWHKKEQDKVEVGDVIAEIETDKAIMEFESIDEGVLAK
CCEEEEHHCCCHHHHCCCCEEEECCCCCCHHHHHHHHHHHHCCHHHHHHHHCCCCCEEE
ILVTEGTSGVPVNQPIALMLEEGEDESALNNYTSTSINSAVKKEVTKSAVDNQKSEHQDL
EEEECCCCCCCCCCCEEEEEECCCCHHHHHCCCHHHHHHHHHHHHHHHHHHCCCHHHCCC
NGKPISHSSVSFQRVTLESNPSMSSQCLTLGSRKEEWIPVSATQMTEGYAEGRTKISPLA
CCCCCCCCCCEEEEEEECCCCCCCHHHHHCCCCCCCCCCCCHHHHHHHHHCCCCHHHHHH
KKIAQNEGVNVQQLKGTGPYGRIIKADVLEFLGSGIHTESPEKDTIVEVSNMRQVIAQRL
HHHHHCCCCCEEEECCCCCCCHHHHHHHHHHHCCCCCCCCCCCCHHEEHHHHHHHHHHHH
TESKQNVPHFYLTVDCQVDKLISLKNEINSADENNKVTINDLIIKAAAFSMKKFPDINSS
HHHHCCCCEEEEEEECCHHHHHHHHHHHCCCCCCCCEEHHHHHHHHHHHHHHHCCCCCCC
WIDNKILRYSNIDISIAVALEDGLITPIVKNADKKGILSISKEVKDLVSRARSGKLKPEE
HHCCCCEEEECCCEEEEEEECCCCCCHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCCHH
FQGGGFTISNLGMFGIKAFSAIINPPQSCIMAVGASKKQPIVMNEKIEIAEIMTVTLSVD
CCCCCEEEECCCHHHHHHHHHHHCCHHHHHHHHCCCCCCCEEECCCEEEEEEEEEEEEEC
HRAVDGALGAKFLNAFKHYIENPLVMLIETAVIY
CHHHHHHHHHHHHHHHHHHHCCCCEEEHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA