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The map label for this gene is ampD [H]

Identifier: 225630805

GI number: 225630805

Start: 1198207

End: 1199784

Strand: Direct

Name: ampD [H]

Synonym: WRi_011090

Alternate gene names: 225630805

Gene position: 1198207-1199784 (Clockwise)

Preceding gene: 225630799

Following gene: 225630807

Centisome position: 82.87

GC content: 31.37

Gene sequence:

>1578_bases
ATGATTTTGTCTTGTAAATTTATTCAACAACCCTATTCTAACTATATAATGAAAAATTATCTACAAAGTGTTTATGAAGA
TGGTATGAAAATCCAACTATCTAAAGTCAACAAATATTTATTAGTTTTACTGATTACTGTCTCAGCATTTCTCATATCTG
GGCAAGTATCCAGTTCAAGCAATATCGAGAATGATTTTCAAGATTTACAAGAAAAATTACCATTATTGAAAGATCAGGAT
TTGTTATTTTTAGATCCAGCTTCAGCTTTAAATTATGGTGATAGGGCAGGTAAAAAAGTGTTAATGGTTATAGTTCACCA
TACTGAAACATCAACACTAAAAGGTACAAAAGATACGCTGAATGCTAGAGGATTGTCAGTTCATTTTATTGTCGATAGGG
ATGGCAACATCACCTTAATGGTTCCATTAGAGAAAGAAGCATGGCATGCTGGTATTAGTTATGCAAGAGTTAAGGTAGAT
AGTAAGCTTGAAGAGTTACGGAAGCTTAACAACTATTCTGTAGGTATTGAAATTGTAAACACAGGACTTGAGCCTTTTCC
GGAAGAGCAAATGAGATCTGTTAAAGAGCTGATTTTATACCTTATGGAGCGTTTTAAAATTAAAAGGGATATGATATTTA
GTCATTCTGAAATAGGAACTATAGTGTATGATCCAGAGCTTGGCTATACAATGCGTAAACCAGATCCACACAAATTGTTT
GATTGGGAGTTATTAGAGAAAAATGAAATTGGATTGCACATAAGTGACAGAATAAACCCTAAAGATGCTAAACACAAAAT
GGGCAAAACATTGTATAAAGCAGGTGATAGAAATGAAGGTATTTTGAAATTAAAACAAAGGCTGAACAGATTTTTCTATA
AGATAGAACCTTGGAATGATAAGAAAGGTAATGTAATTTTTCCTGATAATAATGCTGATTACTCAGATGAGTTTGATGAG
AATTTTGTATGGGTTATTTATCAGTTTTCAATACATAATTTACCAAGAGAAATTAGAAAAGATCTACCTCTTAAACTAGA
ACAAGCAGATATATTTCCTGAATTCTTTAGTGAATATAGTCATGGCATTTCTTCTAGTTATCTAACCTTTAGTGAAAAAA
TTAAATCGACTCTACAACCATGTTTAAGCAAAGTAGATTATGAGAATTTATTATCTTCTCTTGCACAATATGAGAATAAC
ATTTCTCCTGATGCATCTACCACTTTAATGTACAAAATTAAGTTATACTATGATTCTTATTTAAGATATCGCATATGGTC
TTCACTCTATAAACCATTTAAGCTTAATGTATTGGAAGAGTTAGAGATTTTAAAGAGTGGAGTGTTATCTTTAAAATCAC
TAGATAGCTCTAAAGCTGCAGAAGTTTCCAGTTTAATTGATAGCTTTAAAGTAGATATTTCTTTAGAATTTCAGGGTTTT
GAAAAACAGTGGTTTCAGGAGTTTAAGAATGCTTGGAGACAAGAATTTATACCTTCCCTGGAAGAACAGATAACATGGAC
TGCACTACATGAAGCAATATTGGAGTATTTAGAAAAAGCAAAGGAAGAAATTCGTTAA

Upstream 100 bases:

>100_bases
TTTTGATATGCTTTATTGATTTCATTTTGGTTCGCTCCAGAATCTATTCCTAGCACTTTAAATGCTCCATCTCTGAACAT
GTTACTACTACCAGACTAGT

Downstream 100 bases:

>100_bases
ACGAAAACTTTTTTTAAGATCACTTTTTTAACAATTTATCACGTGCCGCATTTAGCTTTTGCGCAAAATATTCCGAGCCT
CCCTTATCTGGGTGAACTAA

Product: N-acetylmuramoyl-L-alanine amidase, family 2

Products: NA

Alternate protein names: N-acetylmuramoyl-L-alanine amidase [H]

Number of amino acids: Translated: 525; Mature: 525

Protein sequence:

>525_residues
MILSCKFIQQPYSNYIMKNYLQSVYEDGMKIQLSKVNKYLLVLLITVSAFLISGQVSSSSNIENDFQDLQEKLPLLKDQD
LLFLDPASALNYGDRAGKKVLMVIVHHTETSTLKGTKDTLNARGLSVHFIVDRDGNITLMVPLEKEAWHAGISYARVKVD
SKLEELRKLNNYSVGIEIVNTGLEPFPEEQMRSVKELILYLMERFKIKRDMIFSHSEIGTIVYDPELGYTMRKPDPHKLF
DWELLEKNEIGLHISDRINPKDAKHKMGKTLYKAGDRNEGILKLKQRLNRFFYKIEPWNDKKGNVIFPDNNADYSDEFDE
NFVWVIYQFSIHNLPREIRKDLPLKLEQADIFPEFFSEYSHGISSSYLTFSEKIKSTLQPCLSKVDYENLLSSLAQYENN
ISPDASTTLMYKIKLYYDSYLRYRIWSSLYKPFKLNVLEELEILKSGVLSLKSLDSSKAAEVSSLIDSFKVDISLEFQGF
EKQWFQEFKNAWRQEFIPSLEEQITWTALHEAILEYLEKAKEEIR

Sequences:

>Translated_525_residues
MILSCKFIQQPYSNYIMKNYLQSVYEDGMKIQLSKVNKYLLVLLITVSAFLISGQVSSSSNIENDFQDLQEKLPLLKDQD
LLFLDPASALNYGDRAGKKVLMVIVHHTETSTLKGTKDTLNARGLSVHFIVDRDGNITLMVPLEKEAWHAGISYARVKVD
SKLEELRKLNNYSVGIEIVNTGLEPFPEEQMRSVKELILYLMERFKIKRDMIFSHSEIGTIVYDPELGYTMRKPDPHKLF
DWELLEKNEIGLHISDRINPKDAKHKMGKTLYKAGDRNEGILKLKQRLNRFFYKIEPWNDKKGNVIFPDNNADYSDEFDE
NFVWVIYQFSIHNLPREIRKDLPLKLEQADIFPEFFSEYSHGISSSYLTFSEKIKSTLQPCLSKVDYENLLSSLAQYENN
ISPDASTTLMYKIKLYYDSYLRYRIWSSLYKPFKLNVLEELEILKSGVLSLKSLDSSKAAEVSSLIDSFKVDISLEFQGF
EKQWFQEFKNAWRQEFIPSLEEQITWTALHEAILEYLEKAKEEIR
>Mature_525_residues
MILSCKFIQQPYSNYIMKNYLQSVYEDGMKIQLSKVNKYLLVLLITVSAFLISGQVSSSSNIENDFQDLQEKLPLLKDQD
LLFLDPASALNYGDRAGKKVLMVIVHHTETSTLKGTKDTLNARGLSVHFIVDRDGNITLMVPLEKEAWHAGISYARVKVD
SKLEELRKLNNYSVGIEIVNTGLEPFPEEQMRSVKELILYLMERFKIKRDMIFSHSEIGTIVYDPELGYTMRKPDPHKLF
DWELLEKNEIGLHISDRINPKDAKHKMGKTLYKAGDRNEGILKLKQRLNRFFYKIEPWNDKKGNVIFPDNNADYSDEFDE
NFVWVIYQFSIHNLPREIRKDLPLKLEQADIFPEFFSEYSHGISSSYLTFSEKIKSTLQPCLSKVDYENLLSSLAQYENN
ISPDASTTLMYKIKLYYDSYLRYRIWSSLYKPFKLNVLEELEILKSGVLSLKSLDSSKAAEVSSLIDSFKVDISLEFQGF
EKQWFQEFKNAWRQEFIPSLEEQITWTALHEAILEYLEKAKEEIR

Specific function: Involved in both cell wall peptidoglycans recycling and beta-lactamase induction. Specifically cleaves the amide bond between the lactyl group of N-acetylmuramic acid and the alpha- amino group of the L-alanine in degradation products containing an anhydr

COG id: COG3023

COG function: function code V; Negative regulator of beta-lactamase expression

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N-acetylmuramoyl-L-alanine amidase 2 family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002502 [H]

Pfam domain/function: PF01510 Amidase_2 [H]

EC number: =3.5.1.28 [H]

Molecular weight: Translated: 61214; Mature: 61214

Theoretical pI: Translated: 6.02; Mature: 6.02

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MILSCKFIQQPYSNYIMKNYLQSVYEDGMKIQLSKVNKYLLVLLITVSAFLISGQVSSSS
CEEECHHHHCHHHHHHHHHHHHHHHHCCCEEEHHHHHHHHHHHHHHHHHHHHHCCCCCCC
NIENDFQDLQEKLPLLKDQDLLFLDPASALNYGDRAGKKVLMVIVHHTETSTLKGTKDTL
CCHHHHHHHHHHCCCCCCCCEEEECCHHHCCCCCCCCCEEEEEEEEECCCCCCCCCHHHH
NARGLSVHFIVDRDGNITLMVPLEKEAWHAGISYARVKVDSKLEELRKLNNYSVGIEIVN
CCCCEEEEEEEECCCCEEEEEECCHHHHHCCCHHEEEEHHHHHHHHHHHCCCEEEEEEEE
TGLEPFPEEQMRSVKELILYLMERFKIKRDMIFSHSEIGTIVYDPELGYTMRKPDPHKLF
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCCCCCCCCCCCC
DWELLEKNEIGLHISDRINPKDAKHKMGKTLYKAGDRNEGILKLKQRLNRFFYKIEPWND
CCEEECCCCCCEEEECCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHEEEECCCCC
KKGNVIFPDNNADYSDEFDENFVWVIYQFSIHNLPREIRKDLPLKLEQADIFPEFFSEYS
CCCCEEECCCCCCCCCCCCCCEEEEEEEEHHHHCCHHHHHCCCCEEHHHHCCHHHHHHHC
HGISSSYLTFSEKIKSTLQPCLSKVDYENLLSSLAQYENNISPDASTTLMYKIKLYYDSY
CCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCCEEEEEEEEHHHHH
LRYRIWSSLYKPFKLNVLEELEILKSGVLSLKSLDSSKAAEVSSLIDSFKVDISLEFQGF
HHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHEEEEEEEECCC
EKQWFQEFKNAWRQEFIPSLEEQITWTALHEAILEYLEKAKEEIR
HHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MILSCKFIQQPYSNYIMKNYLQSVYEDGMKIQLSKVNKYLLVLLITVSAFLISGQVSSSS
CEEECHHHHCHHHHHHHHHHHHHHHHCCCEEEHHHHHHHHHHHHHHHHHHHHHCCCCCCC
NIENDFQDLQEKLPLLKDQDLLFLDPASALNYGDRAGKKVLMVIVHHTETSTLKGTKDTL
CCHHHHHHHHHHCCCCCCCCEEEECCHHHCCCCCCCCCEEEEEEEEECCCCCCCCCHHHH
NARGLSVHFIVDRDGNITLMVPLEKEAWHAGISYARVKVDSKLEELRKLNNYSVGIEIVN
CCCCEEEEEEEECCCCEEEEEECCHHHHHCCCHHEEEEHHHHHHHHHHHCCCEEEEEEEE
TGLEPFPEEQMRSVKELILYLMERFKIKRDMIFSHSEIGTIVYDPELGYTMRKPDPHKLF
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCCCCCCCCCCCC
DWELLEKNEIGLHISDRINPKDAKHKMGKTLYKAGDRNEGILKLKQRLNRFFYKIEPWND
CCEEECCCCCCEEEECCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHEEEECCCCC
KKGNVIFPDNNADYSDEFDENFVWVIYQFSIHNLPREIRKDLPLKLEQADIFPEFFSEYS
CCCCEEECCCCCCCCCCCCCCEEEEEEEEHHHHCCHHHHHCCCCEEHHHHCCHHHHHHHC
HGISSSYLTFSEKIKSTLQPCLSKVDYENLLSSLAQYENNISPDASTTLMYKIKLYYDSY
CCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCCEEEEEEEEHHHHH
LRYRIWSSLYKPFKLNVLEELEILKSGVLSLKSLDSSKAAEVSSLIDSFKVDISLEFQGF
HHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHEEEEEEEECCC
EKQWFQEFKNAWRQEFIPSLEEQITWTALHEAILEYLEKAKEEIR
HHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]