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The map label for this gene is dnaN [H]

Identifier: 225630799

GI number: 225630799

Start: 1191613

End: 1192770

Strand: Direct

Name: dnaN [H]

Synonym: WRi_011020

Alternate gene names: 225630799

Gene position: 1191613-1192770 (Clockwise)

Preceding gene: 225630798

Following gene: 225630805

Centisome position: 82.41

GC content: 34.8

Gene sequence:

>1158_bases
ATGTCAGAGGTTGCAGGTGTAGATACAGAAATCAAAAAGCAAAATTCTGTGTGCGAGCAGATGCGTTTTAGCGTGAGCCG
CACAAGTCTTTTAAATACATTATCCAGAGTAAGTGGAGTGGTTGAAAGGCGTAATTCAATAGATGTTTTGGCGTGTATAA
ATATCAAAGCACAACACGGCAGCATAAAATTAATGGCAACTGATCTTGACATTTCAATGTTTGCCTCACTTGCTGCAACC
GTATTAACAGAAGGAGAAGTAAAAATTTCAGCGCATACTTTACATGACATAGTGAAGAAGTTACCCGCTGATTTGGATGT
CAATTTTGAAGTAAACAACCAAGGAAAATTATTGATGTCTTGCGGAAATGCAAACTTTTCTTTACCGAATGTAGTTTCAA
GCAGCTTTCCTGCTCTTGAAGAAGACAATTATAAACATGATTTTATTCTACTAAATACGGACCTGATAGACTTATTAACT
AAAACAAAATTTGCTGTATCACTAGATGATACAAGGTATAATTTAAATGGAATATATCTACATACAGATGAGCAGTTTCT
GTACTGCGTTGCAACTGATGGCCATCGTTTATCATGTATAAAGAGGCCAAAGCCTGGGAACATCAATGGCGAATTTGGTG
TGATCATTCCACGTAAAACTGTTATGGAGCTGTTAAAAATATTGGACGATTGTAATGAAATTAAAGTAAAACTCTCAGAC
AGAAAAATCAAGCTTACATGCGGTGAATATATTCTAATATCAAAATTAATAGATGGGACTTTTCCAAATTATAAAGCAGT
TATTCCAGCATCTCAAGATAAGCATATGATTATTGAAAGTAAAAAACTTTCAGATGTTATAGATCGAGTTTCCGTTGTTG
TATCTGATAAAGTAAAATCTATTAAATTCTCACTACAAGAAAAGCTGCTAACTCTGCATTCAAACTCCCAAGAGTGCAGT
GATGCAACTGAATCCATAGAAGTGGACTACAATGAAGCCCCTATAGAAATAGGGTTCAACTCGCGTTATTTGCTTGATGT
TCTATCTTGCATAAAAAATAAATGTAAGTTTAGCTTAGCCGATGGTAATAGTGCTACAATTATCACTGATGAAGATGATT
CAAGTGTATTATATGTAGTGATGCCAATGAGAACTTAA

Upstream 100 bases:

>100_bases
TATAGTATAAACTAGACTACTCTTTCAATGGAAATATAAATAGCACTTTACTTTATATGGGATGTGGATATCATTTAAAA
TATATTTTTGAGAGTTAGCA

Downstream 100 bases:

>100_bases
ACTCGTCATCTCGCTGCTTGTTAGCGAGATCTGGAGATACTGCGGCGGTATGACACTTATTTCACTAAGAACGTTACTTT
TTATGTATCCGTTCAGGAGA

Product: DNA polymerase III, beta subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 385; Mature: 384

Protein sequence:

>385_residues
MSEVAGVDTEIKKQNSVCEQMRFSVSRTSLLNTLSRVSGVVERRNSIDVLACINIKAQHGSIKLMATDLDISMFASLAAT
VLTEGEVKISAHTLHDIVKKLPADLDVNFEVNNQGKLLMSCGNANFSLPNVVSSSFPALEEDNYKHDFILLNTDLIDLLT
KTKFAVSLDDTRYNLNGIYLHTDEQFLYCVATDGHRLSCIKRPKPGNINGEFGVIIPRKTVMELLKILDDCNEIKVKLSD
RKIKLTCGEYILISKLIDGTFPNYKAVIPASQDKHMIIESKKLSDVIDRVSVVVSDKVKSIKFSLQEKLLTLHSNSQECS
DATESIEVDYNEAPIEIGFNSRYLLDVLSCIKNKCKFSLADGNSATIITDEDDSSVLYVVMPMRT

Sequences:

>Translated_385_residues
MSEVAGVDTEIKKQNSVCEQMRFSVSRTSLLNTLSRVSGVVERRNSIDVLACINIKAQHGSIKLMATDLDISMFASLAAT
VLTEGEVKISAHTLHDIVKKLPADLDVNFEVNNQGKLLMSCGNANFSLPNVVSSSFPALEEDNYKHDFILLNTDLIDLLT
KTKFAVSLDDTRYNLNGIYLHTDEQFLYCVATDGHRLSCIKRPKPGNINGEFGVIIPRKTVMELLKILDDCNEIKVKLSD
RKIKLTCGEYILISKLIDGTFPNYKAVIPASQDKHMIIESKKLSDVIDRVSVVVSDKVKSIKFSLQEKLLTLHSNSQECS
DATESIEVDYNEAPIEIGFNSRYLLDVLSCIKNKCKFSLADGNSATIITDEDDSSVLYVVMPMRT
>Mature_384_residues
SEVAGVDTEIKKQNSVCEQMRFSVSRTSLLNTLSRVSGVVERRNSIDVLACINIKAQHGSIKLMATDLDISMFASLAATV
LTEGEVKISAHTLHDIVKKLPADLDVNFEVNNQGKLLMSCGNANFSLPNVVSSSFPALEEDNYKHDFILLNTDLIDLLTK
TKFAVSLDDTRYNLNGIYLHTDEQFLYCVATDGHRLSCIKRPKPGNINGEFGVIIPRKTVMELLKILDDCNEIKVKLSDR
KIKLTCGEYILISKLIDGTFPNYKAVIPASQDKHMIIESKKLSDVIDRVSVVVSDKVKSIKFSLQEKLLTLHSNSQECSD
ATESIEVDYNEAPIEIGFNSRYLLDVLSCIKNKCKFSLADGNSATIITDEDDSSVLYVVMPMRT

Specific function: DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity. The beta chain is required for initiation of replication once it is clamped

COG id: COG0592

COG function: function code L; DNA polymerase sliding clamp subunit (PCNA homolog)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1790136, Length=367, Percent_Identity=33.2425068119891, Blast_Score=221, Evalue=5e-59,

Paralogues:

None

Copy number: 5954 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001001
- InterPro:   IPR022635
- InterPro:   IPR022637
- InterPro:   IPR022634 [H]

Pfam domain/function: PF00712 DNA_pol3_beta; PF02767 DNA_pol3_beta_2; PF02768 DNA_pol3_beta_3 [H]

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 42812; Mature: 42681

Theoretical pI: Translated: 5.68; Mature: 5.68

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEVAGVDTEIKKQNSVCEQMRFSVSRTSLLNTLSRVSGVVERRNSIDVLACINIKAQHG
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEEECCC
SIKLMATDLDISMFASLAATVLTEGEVKISAHTLHDIVKKLPADLDVNFEVNNQGKLLMS
EEEEEEECCCHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHCCCCEEEEEEECCCCEEEEE
CGNANFSLPNVVSSSFPALEEDNYKHDFILLNTDLIDLLTKTKFAVSLDDTRYNLNGIYL
ECCCCCCCCHHHHCCCCCCCCCCCCCCEEEEECHHHHHHHCCEEEEEECCCEEEEEEEEE
HTDEQFLYCVATDGHRLSCIKRPKPGNINGEFGVIIPRKTVMELLKILDDCNEIKVKLSD
EECCCEEEEEECCCCEEEEEECCCCCCCCCEEEEEECHHHHHHHHHHHCCCCEEEEEEEC
RKIKLTCGEYILISKLIDGTFPNYKAVIPASQDKHMIIESKKLSDVIDRVSVVVSDKVKS
CEEEEEECCEEEEHHHHCCCCCCCEEEEECCCCCEEEEECHHHHHHHHHHHHHHHHHHHH
IKFSLQEKLLTLHSNSQECSDATESIEVDYNEAPIEIGFNSRYLLDVLSCIKNKCKFSLA
HHHHHHHHHHHHCCCCHHHCCCCCEEEEECCCCCEEECCCCHHHHHHHHHHHHHCEEEEC
DGNSATIITDEDDSSVLYVVMPMRT
CCCEEEEEECCCCCCEEEEEEECCC
>Mature Secondary Structure 
SEVAGVDTEIKKQNSVCEQMRFSVSRTSLLNTLSRVSGVVERRNSIDVLACINIKAQHG
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEEECCC
SIKLMATDLDISMFASLAATVLTEGEVKISAHTLHDIVKKLPADLDVNFEVNNQGKLLMS
EEEEEEECCCHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHCCCCEEEEEEECCCCEEEEE
CGNANFSLPNVVSSSFPALEEDNYKHDFILLNTDLIDLLTKTKFAVSLDDTRYNLNGIYL
ECCCCCCCCHHHHCCCCCCCCCCCCCCEEEEECHHHHHHHCCEEEEEECCCEEEEEEEEE
HTDEQFLYCVATDGHRLSCIKRPKPGNINGEFGVIIPRKTVMELLKILDDCNEIKVKLSD
EECCCEEEEEECCCCEEEEEECCCCCCCCCEEEEEECHHHHHHHHHHHCCCCEEEEEEEC
RKIKLTCGEYILISKLIDGTFPNYKAVIPASQDKHMIIESKKLSDVIDRVSVVVSDKVKS
CEEEEEECCEEEEHHHHCCCCCCCEEEEECCCCCEEEEECHHHHHHHHHHHHHHHHHHHH
IKFSLQEKLLTLHSNSQECSDATESIEVDYNEAPIEIGFNSRYLLDVLSCIKNKCKFSLA
HHHHHHHHHHHHCCCCHHHCCCCCEEEEECCCCCEEECCCCHHHHHHHHHHHHHCEEEEC
DGNSATIITDEDDSSVLYVVMPMRT
CCCEEEEEECCCCCCEEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9079919; 8226640 [H]