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The map label for this gene is nifU [H]

Identifier: 225630588

GI number: 225630588

Start: 925737

End: 926120

Strand: Reverse

Name: nifU [H]

Synonym: WRi_008540

Alternate gene names: 225630588

Gene position: 926120-925737 (Counterclockwise)

Preceding gene: 225630590

Following gene: 225630587

Centisome position: 64.05

GC content: 36.46

Gene sequence:

>384_bases
ATGAGTTATAATGAGAAAATTTTAGATCATTACGAAAATCCAAGAAATGTTGGCTCTCTGGATAAAAATGATCCAAATGT
TGGCACTGGTCTAGTTGGTGCACCATCGTGCGGTGATGTAATGAAATTGCAGATAAAGGTCAATGACAAAGGTGTTATTG
AAGATGCAAAATTTAAAACTTTTGGCTGCGGTTCTGCCATTGCTTCAAGTTCCTTGTTAACAGAGATGATAAAGGGAAGA
ACCATCAGTGACGTTACACAGATAAAGAATACTCAAATAGTGGAAGAGTTGTCTTTACCTCCAGTGAAGATACACTGTTC
GGTACTTGCTGAAGATGCTATAAAGGCTGCTATTCATGACTATCAAAGTAAACAAAAGAATTAA

Upstream 100 bases:

>100_bases
ATATATTTTGTGAGTAAAATATACTGTTTGCTAGTGCATTTTTCTTTAGTTTTTAACCTAAATATGTTATAATGTAGAAT
AGCGGTTCACAGGTAATTTT

Downstream 100 bases:

>100_bases
GAAGCTACGAACATGGGTGAATATCAAGAAGTAGGCGGAGGTGAAACTGTAAAAAAGAATAAAAAACTTATCACTATAAC
TGCGGAAGCAGTAGAAAGGA

Product: scaffold protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 127; Mature: 126

Protein sequence:

>127_residues
MSYNEKILDHYENPRNVGSLDKNDPNVGTGLVGAPSCGDVMKLQIKVNDKGVIEDAKFKTFGCGSAIASSSLLTEMIKGR
TISDVTQIKNTQIVEELSLPPVKIHCSVLAEDAIKAAIHDYQSKQKN

Sequences:

>Translated_127_residues
MSYNEKILDHYENPRNVGSLDKNDPNVGTGLVGAPSCGDVMKLQIKVNDKGVIEDAKFKTFGCGSAIASSSLLTEMIKGR
TISDVTQIKNTQIVEELSLPPVKIHCSVLAEDAIKAAIHDYQSKQKN
>Mature_126_residues
SYNEKILDHYENPRNVGSLDKNDPNVGTGLVGAPSCGDVMKLQIKVNDKGVIEDAKFKTFGCGSAIASSSLLTEMIKGRT
ISDVTQIKNTQIVEELSLPPVKIHCSVLAEDAIKAAIHDYQSKQKN

Specific function: May be involved in the formation or repair of [Fe-S] clusters present in iron-sulfur proteins (Potential) [H]

COG id: COG0822

COG function: function code C; NifU homolog involved in Fe-S cluster formation

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nifU family [H]

Homologues:

Organism=Homo sapiens, GI56699456, Length=124, Percent_Identity=71.7741935483871, Blast_Score=196, Evalue=6e-51,
Organism=Homo sapiens, GI24307953, Length=121, Percent_Identity=71.900826446281, Blast_Score=189, Evalue=5e-49,
Organism=Escherichia coli, GI1788878, Length=126, Percent_Identity=73.8095238095238, Blast_Score=209, Evalue=3e-56,
Organism=Caenorhabditis elegans, GI17543474, Length=123, Percent_Identity=73.1707317073171, Blast_Score=197, Evalue=1e-51,
Organism=Saccharomyces cerevisiae, GI6325122, Length=124, Percent_Identity=73.3870967741936, Blast_Score=197, Evalue=3e-52,
Organism=Saccharomyces cerevisiae, GI6324800, Length=124, Percent_Identity=68.5483870967742, Blast_Score=189, Evalue=2e-49,
Organism=Drosophila melanogaster, GI21355597, Length=124, Percent_Identity=70.9677419354839, Blast_Score=191, Evalue=8e-50,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011339
- InterPro:   IPR002871 [H]

Pfam domain/function: PF01592 NifU_N [H]

EC number: NA

Molecular weight: Translated: 13775; Mature: 13644

Theoretical pI: Translated: 6.77; Mature: 6.77

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSYNEKILDHYENPRNVGSLDKNDPNVGTGLVGAPSCGDVMKLQIKVNDKGVIEDAKFKT
CCCCHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEEECCCCCCCCCCCCC
FGCGSAIASSSLLTEMIKGRTISDVTQIKNTQIVEELSLPPVKIHCSVLAEDAIKAAIHD
CCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHH
YQSKQKN
HHHHCCC
>Mature Secondary Structure 
SYNEKILDHYENPRNVGSLDKNDPNVGTGLVGAPSCGDVMKLQIKVNDKGVIEDAKFKT
CCCHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEEECCCCCCCCCCCCC
FGCGSAIASSSLLTEMIKGRTISDVTQIKNTQIVEELSLPPVKIHCSVLAEDAIKAAIHD
CCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHH
YQSKQKN
HHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]