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The map label for this gene is dnaX [H]

Identifier: 225630532

GI number: 225630532

Start: 845658

End: 846479

Strand: Reverse

Name: dnaX [H]

Synonym: WRi_007850

Alternate gene names: 225630532

Gene position: 846479-845658 (Counterclockwise)

Preceding gene: 225630533

Following gene: 225630531

Centisome position: 58.54

GC content: 30.9

Gene sequence:

>822_bases
ATGAAAAAAGTAATAGGCCACAACCAAGCCAAAAAAAAAATAATGAACAACTTATCTGTTCAGTCTTGGCTGATCTGTGG
TAAAAAAGGTATAGGAAAAGCAACACTTGCAAAATCCTTCTCCAATTGGTTACTCATAAAAAACTATAATGAAGTAGCAT
TGGACTTACACATTGTTGAAGGTGATACTATCGGAATAGAAAAAGTCAGAGAAATGAAAAACTTTCTACACTTAAGTCCC
ATTCAATCAGAACACAAAATAGCTATAATAGATAGCTTAGAGGCAATGACTAATAACGCTAAAAACGCAATATTGAAAAT
ATTAGAAGAGCCGCCAAAAAATTCTAAGATATTTATAATTAGCCATAAGCCATATGATATACAAACCACTATCTTGTGTA
GGTGCTTTCAGCTGAATTTACTTCCATTAACTTATGATGAAACAAAGCAAGTTGTTTCATCTCAATGTAAATTTGATAAT
CAAACGTTTGACGAAATGATTACTTTATTTCCTGGAATGCCAGGAATGATAATAAATACAATAAGTAGTGATGCTTATGA
AGCATACAAATGCTTTTATAAATTTCTTCATGATTTAAAGAATCCCAATATGATTAATAAAGTAATTAATAGCGAAATCG
AACTAGAACTAGCATCATATATAATTCAGGCCTTCATTTTAGAAAGCATAAAGAGAGAGGTAAATGGTGTTGAAATTCTG
CTAAGTCAGTGGAAAAAAATAGATGAACTTTTCGTTGCCGCAAAGCAACTCCACTTAGATAAAAAGCATGTTTTAGCTAA
TGTAGTTAATATCATGACATAA

Upstream 100 bases:

>100_bases
TTGACTTAACCGAAGATAAGATTAAGGTTTTAAGGGAAGGTGCAATTTCATTACAAACAGTAAACAACGCATTTTCAAAC
TAAAATCTATAGGAGAAAGA

Downstream 100 bases:

>100_bases
GTTTACAAAGTATAATACTTTTAACATAAATTCATGATAAAATTCTGTTAATTTAATACTCGAAGTATATAATTTGACTG
ATAATAAATTAACTGTATGG

Product: DNA polymerase III, delta' subunit, putative

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 273; Mature: 273

Protein sequence:

>273_residues
MKKVIGHNQAKKKIMNNLSVQSWLICGKKGIGKATLAKSFSNWLLIKNYNEVALDLHIVEGDTIGIEKVREMKNFLHLSP
IQSEHKIAIIDSLEAMTNNAKNAILKILEEPPKNSKIFIISHKPYDIQTTILCRCFQLNLLPLTYDETKQVVSSQCKFDN
QTFDEMITLFPGMPGMIINTISSDAYEAYKCFYKFLHDLKNPNMINKVINSEIELELASYIIQAFILESIKREVNGVEIL
LSQWKKIDELFVAAKQLHLDKKHVLANVVNIMT

Sequences:

>Translated_273_residues
MKKVIGHNQAKKKIMNNLSVQSWLICGKKGIGKATLAKSFSNWLLIKNYNEVALDLHIVEGDTIGIEKVREMKNFLHLSP
IQSEHKIAIIDSLEAMTNNAKNAILKILEEPPKNSKIFIISHKPYDIQTTILCRCFQLNLLPLTYDETKQVVSSQCKFDN
QTFDEMITLFPGMPGMIINTISSDAYEAYKCFYKFLHDLKNPNMINKVINSEIELELASYIIQAFILESIKREVNGVEIL
LSQWKKIDELFVAAKQLHLDKKHVLANVVNIMT
>Mature_273_residues
MKKVIGHNQAKKKIMNNLSVQSWLICGKKGIGKATLAKSFSNWLLIKNYNEVALDLHIVEGDTIGIEKVREMKNFLHLSP
IQSEHKIAIIDSLEAMTNNAKNAILKILEEPPKNSKIFIISHKPYDIQTTILCRCFQLNLLPLTYDETKQVVSSQCKFDN
QTFDEMITLFPGMPGMIINTISSDAYEAYKCFYKFLHDLKNPNMINKVINSEIELELASYIIQAFILESIKREVNGVEIL
LSQWKKIDELFVAAKQLHLDKKHVLANVVNIMT

Specific function: DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity [H]

COG id: COG0470

COG function: function code L; ATPase involved in DNA replication

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1786676, Length=167, Percent_Identity=25.1497005988024, Blast_Score=67, Evalue=9e-13,
Organism=Saccharomyces cerevisiae, GI6324039, Length=208, Percent_Identity=24.0384615384615, Blast_Score=65, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR003959
- InterPro:   IPR008921
- InterPro:   IPR022754
- InterPro:   IPR012763 [H]

Pfam domain/function: PF00004 AAA; PF12169 DNA_pol3_gamma3 [H]

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 31194; Mature: 31194

Theoretical pI: Translated: 8.68; Mature: 8.68

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKVIGHNQAKKKIMNNLSVQSWLICGKKGIGKATLAKSFSNWLLIKNYNEVALDLHIVE
CCCCCCCCHHHHHHHHCCCHHHHHEECCCCCCHHHHHHHHHCEEEEECCCEEEEEEEEEC
GDTIGIEKVREMKNFLHLSPIQSEHKIAIIDSLEAMTNNAKNAILKILEEPPKNSKIFII
CCCCCHHHHHHHHHHHHCCCCCCCCCEEEEHHHHHHHCHHHHHHHHHHHCCCCCCEEEEE
SHKPYDIQTTILCRCFQLNLLPLTYDETKQVVSSQCKFDNQTFDEMITLFPGMPGMIINT
ECCCCCHHHHHHHHHHHCCCEECCHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCHHHHH
ISSDAYEAYKCFYKFLHDLKNPNMINKVINSEIELELASYIIQAFILESIKREVNGVEIL
HCHHHHHHHHHHHHHHHHCCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LSQWKKIDELFVAAKQLHLDKKHVLANVVNIMT
HHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHCC
>Mature Secondary Structure
MKKVIGHNQAKKKIMNNLSVQSWLICGKKGIGKATLAKSFSNWLLIKNYNEVALDLHIVE
CCCCCCCCHHHHHHHHCCCHHHHHEECCCCCCHHHHHHHHHCEEEEECCCEEEEEEEEEC
GDTIGIEKVREMKNFLHLSPIQSEHKIAIIDSLEAMTNNAKNAILKILEEPPKNSKIFII
CCCCCHHHHHHHHHHHHCCCCCCCCCEEEEHHHHHHHCHHHHHHHHHHHCCCCCCEEEEE
SHKPYDIQTTILCRCFQLNLLPLTYDETKQVVSSQCKFDNQTFDEMITLFPGMPGMIINT
ECCCCCHHHHHHHHHHHCCCEECCHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCHHHHH
ISSDAYEAYKCFYKFLHDLKNPNMINKVINSEIELELASYIIQAFILESIKREVNGVEIL
HCHHHHHHHHHHHHHHHHCCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LSQWKKIDELFVAAKQLHLDKKHVLANVVNIMT
HHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10993077 [H]