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The map label for this gene is rho [H]

Identifier: 225630405

GI number: 225630405

Start: 685893

End: 687296

Strand: Reverse

Name: rho [H]

Synonym: WRi_006360

Alternate gene names: 225630405

Gene position: 687296-685893 (Counterclockwise)

Preceding gene: 225630406

Following gene: 225630402

Centisome position: 47.54

GC content: 35.97

Gene sequence:

>1404_bases
ATGACAACAATCAATGAAGATGTTTTGGCAAAAGGAAAGGTTACAGCTCAACCTGAAAAAAGTGAGCAAATTTACAATAA
CGATACAGTAAAACAGATGGTCAATGAAGGTACTGAGAAAAACAGAAAAACATTAGATCTGAGCGAATTAAAAGAGAAAA
CAGCAGAAGAATTGTTAGAGTTAGCTGAAGAAAGAAAAATTTCAACTAACGGTAAAAGCAATGGTAGAATGCTAAAGCAG
GAAATAATATTCAGCTTAATGAAAAAAATGAGTGAAGAAGGAGGCATAACCACAGGGAGTGGAATAGTGGAAATATTGCC
TGATGGTTTTGGTTTCTTACGTTCAGCAAGTGCAAATTATGCCCCAAGTACCGATGATGTTTATATTTCTAACGGGCAAA
TAAAGAAGTTTAATTTACGTACAGGAGATATGGCATATGGAGAAATAAGGCCACCTGGTGATAAAGAGAGATATTTTACT
TTAACTAAGGTTCAAAGTATAAACTCTACTGAAGTAAGTGAATTAAGAAAGTACGTCCATTTTGATAATTTGCTTCCTCT
TTATCCTGAGGAAAGCATAGTACTTGAGTGTAATAATGGTGATGATAAAAAAGGCTTAAGCATGCGTGCTATAGATATAG
TAGCTCCTCTTGGAAAAGGGCAAAGAGCGTTAGTAGTTGCTCCACCTCGCACAGGAAAAACTGTATTGCTTCAGCAAATG
GCTAACTCTATAGCTATAAATCACCCTGAAGTAGAATTAATAGTGTTACTTATAGATGAAAGACCTGAAGAAGTAACAGA
TATGATACGTTCTGTAAAAGGTGAGGTAGTAAGTTCTACGTTCGATGAGCCTGCTTACCGTCACGTACAGCTTGCTGAAA
TAGTAATAGAAAAAGCTAAAAGAATGGTTGAGCATAAAAAAGATGTGGTAATTCTACTTGACAATATTACTAGACTTGCA
CGTGCTTATAATGCGGTTATTCCTTCGTCTGGGAAAGTTCTAACTGGTGGTGTAGATTCAAACGCACTGCAGAGACCAAA
ACGCTTTTTTGGAGCAGCTCGTAATATTGAGAATGGGGGTTCTTTAACTATAATCGCCACAGCTCTTATAGAGACTGGTT
CAAAAATGGATGAAGTTATTTTTGAAGAGTTTAAAGGTACAGGAAATGCTGAGATCATACTTGATAGAAAACTTTCTGAT
AAACGTATATTTCCAGCTATTGATATTACAAAATCCGGAACAAGGAAGGAAGAGCTATTAGTTGATAAGGCTATACTAAA
TAAAATATGGGTGGTGCGTAGGATACTCAATCCTATGGGACCCGTTGAAGCAATGGAATTTTTACGTGACAAACTACTTT
TAACAAAGAGCAATGCTGACTTTTTTAACTCCATGAATCACTAA

Upstream 100 bases:

>100_bases
ATAATTAAGCTTAATACGCACTGAAACTAACTCAGCCTAATTTATTATCTTATAGTCACATTTTAGGTTAGTAAGCATCA
ATTAAAATTTTTGGAGGATT

Downstream 100 bases:

>100_bases
AAGTAAGCCAGTTATGATGGATATGCTGAGCAAAAAGTAAAACGCTCTTATAGCTAAAATAATTTGGATTTACCAAAATA
TATTTATAGTTAAGTTGGCA

Product: transcription termination factor Rho

Products: NA

Alternate protein names: ATP-dependent helicase Rho 1 [H]

Number of amino acids: Translated: 467; Mature: 466

Protein sequence:

>467_residues
MTTINEDVLAKGKVTAQPEKSEQIYNNDTVKQMVNEGTEKNRKTLDLSELKEKTAEELLELAEERKISTNGKSNGRMLKQ
EIIFSLMKKMSEEGGITTGSGIVEILPDGFGFLRSASANYAPSTDDVYISNGQIKKFNLRTGDMAYGEIRPPGDKERYFT
LTKVQSINSTEVSELRKYVHFDNLLPLYPEESIVLECNNGDDKKGLSMRAIDIVAPLGKGQRALVVAPPRTGKTVLLQQM
ANSIAINHPEVELIVLLIDERPEEVTDMIRSVKGEVVSSTFDEPAYRHVQLAEIVIEKAKRMVEHKKDVVILLDNITRLA
RAYNAVIPSSGKVLTGGVDSNALQRPKRFFGAARNIENGGSLTIIATALIETGSKMDEVIFEEFKGTGNAEIILDRKLSD
KRIFPAIDITKSGTRKEELLVDKAILNKIWVVRRILNPMGPVEAMEFLRDKLLLTKSNADFFNSMNH

Sequences:

>Translated_467_residues
MTTINEDVLAKGKVTAQPEKSEQIYNNDTVKQMVNEGTEKNRKTLDLSELKEKTAEELLELAEERKISTNGKSNGRMLKQ
EIIFSLMKKMSEEGGITTGSGIVEILPDGFGFLRSASANYAPSTDDVYISNGQIKKFNLRTGDMAYGEIRPPGDKERYFT
LTKVQSINSTEVSELRKYVHFDNLLPLYPEESIVLECNNGDDKKGLSMRAIDIVAPLGKGQRALVVAPPRTGKTVLLQQM
ANSIAINHPEVELIVLLIDERPEEVTDMIRSVKGEVVSSTFDEPAYRHVQLAEIVIEKAKRMVEHKKDVVILLDNITRLA
RAYNAVIPSSGKVLTGGVDSNALQRPKRFFGAARNIENGGSLTIIATALIETGSKMDEVIFEEFKGTGNAEIILDRKLSD
KRIFPAIDITKSGTRKEELLVDKAILNKIWVVRRILNPMGPVEAMEFLRDKLLLTKSNADFFNSMNH
>Mature_466_residues
TTINEDVLAKGKVTAQPEKSEQIYNNDTVKQMVNEGTEKNRKTLDLSELKEKTAEELLELAEERKISTNGKSNGRMLKQE
IIFSLMKKMSEEGGITTGSGIVEILPDGFGFLRSASANYAPSTDDVYISNGQIKKFNLRTGDMAYGEIRPPGDKERYFTL
TKVQSINSTEVSELRKYVHFDNLLPLYPEESIVLECNNGDDKKGLSMRAIDIVAPLGKGQRALVVAPPRTGKTVLLQQMA
NSIAINHPEVELIVLLIDERPEEVTDMIRSVKGEVVSSTFDEPAYRHVQLAEIVIEKAKRMVEHKKDVVILLDNITRLAR
AYNAVIPSSGKVLTGGVDSNALQRPKRFFGAARNIENGGSLTIIATALIETGSKMDEVIFEEFKGTGNAEIILDRKLSDK
RIFPAIDITKSGTRKEELLVDKAILNKIWVVRRILNPMGPVEAMEFLRDKLLLTKSNADFFNSMNH

Specific function: Facilitates transcription termination by a mechanism that involves Rho binding to the nascent RNA, activation of Rho's RNA-dependent ATPase activity, and release of the mRNA from the DNA template [H]

COG id: COG1158

COG function: function code K; Transcription termination factor

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Rho family [H]

Homologues:

Organism=Escherichia coli, GI1790217, Length=421, Percent_Identity=59.8574821852732, Blast_Score=521, Evalue=1e-149,

Paralogues:

None

Copy number: 300 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000194
- InterPro:   IPR003593
- InterPro:   IPR011129
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR011112
- InterPro:   IPR011113
- InterPro:   IPR004665 [H]

Pfam domain/function: PF00006 ATP-synt_ab; PF07498 Rho_N; PF07497 Rho_RNA_bind [H]

EC number: NA

Molecular weight: Translated: 52042; Mature: 51910

Theoretical pI: Translated: 6.42; Mature: 6.42

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTINEDVLAKGKVTAQPEKSEQIYNNDTVKQMVNEGTEKNRKTLDLSELKEKTAEELLE
CCCCCCHHHCCCCCCCCCCHHHHHCCCHHHHHHHHCCCHHCCCCCCHHHHHHHHHHHHHH
LAEERKISTNGKSNGRMLKQEIIFSLMKKMSEEGGITTGSGIVEILPDGFGFLRSASANY
HHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCHHHHHCCCCCC
APSTDDVYISNGQIKKFNLRTGDMAYGEIRPPGDKERYFTLTKVQSINSTEVSELRKYVH
CCCCCCEEEECCEEEEEECCCCCCCCCCCCCCCCCCCEEEEEEECCCCCHHHHHHHHHHH
FDNLLPLYPEESIVLECNNGDDKKGLSMRAIDIVAPLGKGQRALVVAPPRTGKTVLLQQM
HCCCCCCCCCCCEEEEECCCCCCCCCEEEEEEEEECCCCCCEEEEEECCCCCHHHHHHHH
ANSIAINHPEVELIVLLIDERPEEVTDMIRSVKGEVVSSTFDEPAYRHVQLAEIVIEKAK
HHHHCCCCCCEEEEEEEECCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHH
RMVEHKKDVVILLDNITRLARAYNAVIPSSGKVLTGGVDSNALQRPKRFFGAARNIENGG
HHHHCCCCEEEEEHHHHHHHHHHHHCCCCCCCEEECCCCCHHHHHHHHHHHHHCCCCCCC
SLTIIATALIETGSKMDEVIFEEFKGTGNAEIILDRKLSDKRIFPAIDITKSGTRKEELL
CEEEEEEHHHHCCCHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCEEECCCCCCCHHHHH
VDKAILNKIWVVRRILNPMGPVEAMEFLRDKLLLTKSNADFFNSMNH
HHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHEEEECCCHHHHCCCC
>Mature Secondary Structure 
TTINEDVLAKGKVTAQPEKSEQIYNNDTVKQMVNEGTEKNRKTLDLSELKEKTAEELLE
CCCCCHHHCCCCCCCCCCHHHHHCCCHHHHHHHHCCCHHCCCCCCHHHHHHHHHHHHHH
LAEERKISTNGKSNGRMLKQEIIFSLMKKMSEEGGITTGSGIVEILPDGFGFLRSASANY
HHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCHHHHHCCCCCC
APSTDDVYISNGQIKKFNLRTGDMAYGEIRPPGDKERYFTLTKVQSINSTEVSELRKYVH
CCCCCCEEEECCEEEEEECCCCCCCCCCCCCCCCCCCEEEEEEECCCCCHHHHHHHHHHH
FDNLLPLYPEESIVLECNNGDDKKGLSMRAIDIVAPLGKGQRALVVAPPRTGKTVLLQQM
HCCCCCCCCCCCEEEEECCCCCCCCCEEEEEEEEECCCCCCEEEEEECCCCCHHHHHHHH
ANSIAINHPEVELIVLLIDERPEEVTDMIRSVKGEVVSSTFDEPAYRHVQLAEIVIEKAK
HHHHCCCCCCEEEEEEEECCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHH
RMVEHKKDVVILLDNITRLARAYNAVIPSSGKVLTGGVDSNALQRPKRFFGAARNIENGG
HHHHCCCCEEEEEHHHHHHHHHHHHCCCCCCCEEECCCCCHHHHHHHHHHHHHCCCCCCC
SLTIIATALIETGSKMDEVIFEEFKGTGNAEIILDRKLSDKRIFPAIDITKSGTRKEELL
CEEEEEEHHHHCCCHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCEEECCCCCCCHHHHH
VDKAILNKIWVVRRILNPMGPVEAMEFLRDKLLLTKSNADFFNSMNH
HHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHEEEECCCHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA