Click here to switch to the map view.

The map label for this gene is gltX [H]

Identifier: 225630392

GI number: 225630392

Start: 668247

End: 669569

Strand: Direct

Name: gltX [H]

Synonym: WRi_006210

Alternate gene names: 225630392

Gene position: 668247-669569 (Clockwise)

Preceding gene: 225630390

Following gene: 225630393

Centisome position: 46.22

GC content: 36.36

Gene sequence:

>1323_bases
ATGCTAACAAGATTCGCTCCAAGCCCAACTGGCTACCTCCACGTAGGAAACGTACGAACTGCCTTGGTTTGTTGGATGTA
CACACGCAATCAAAATGGAAAATTTTTACTCCGTTTTGATGATACTGACCTTCAGCGTTCAGATGTCAAATATATAAATG
ATATCATACAAGATTTGAAATGGATTGGTATCAATTGGGATGCAAGTTTCAAGCAATCAGAGCGCTTTGAGCGTTACAAT
GAGGTGTTTTTGCAGTTAATAAAAGAAGGGCATATTTATGCATGCTATGAAACAAGAGAAGAGTTAGACATTAAACGAAA
ATTGCAGTTAAAACAAGGACTTCCCCCTGTATATGACAGAAGCGCGTTACTTCTAACTGAGCAAGAGAAAATTAGTTATG
AGCAAGAAGGAAGAAGACCACATTTTAGATTTAAGTTGGATAGAAATGAGGTTGTTAAATGGAATGATGAAGTTAAAGAT
GAAATAAATATTGCAACCAGTAGCATAAGTGACCCTGTGGTAAAAAGAGAAGATGGAATTTACACATACATGTTACCTTC
TGTTATTGATGATGTTGACTTTAATGTAACCCATGTTGTACGCGGGGAAGATCATGTAACTAATACCGCAGTTCAGCTCC
AAATGATTAAAGCATTAAAAGCGAAAATCCCTACATTTGCTCACCTTTCTCTGCTACATTTTGATGATAGCAAGATATCC
AAGCGAACCGGCGTGCTGAACATTAAATCTATAAAAGAAGATGAAATTGAACCGATGGCACTAGTTAGTTATTTAGTGAA
GCTTGGAACATCCAATCCAATTGAAGCTTGCGCCTGTATGCAATCTTTGATTGATTCATTTAATATTAAAAAATTTAGCT
CAGCATCTGTACAATTCAGCTTGAGTGAAATATATAAGCTAAATAGCAAAGTTCTGCAACAAATGCCATTTGAAATGGTG
CAAGACCGTTTAAATCAAATTGGAGTGGACTCCTCAGAGTTTTGGTATTTTATAAGGAACAATATAGAAAGGTTTTCTGA
GGTGGCCAAGTGGTGGAAAATATGCAAATCTGATATAGAGCCTGTGATTCTTGATAAGGAGCTTATAAAAATTGCGTTCA
ATGCATTGCCCCAAGGTGATTGTAATGAAAACACGTTGTCAGAATGGGTCAAAACTATTCGACAAACAGTGGATATAAAG
GCAAAAAACCTATTTACGCAGTTGCGTTCAGCTTTAACAGGAACAGAAACAGGACCAGAGCTCGCTAAACTATTAATTTT
TATCGGCAAAGAAAATATCATTGCAAGGTTAAAAGAAAAGTGA

Upstream 100 bases:

>100_bases
TATTCTTCTCTCGCTCAATTGTATTAGCAGATCTCACTTGCTTTAGCCATATATTTATATAAAATTAAAATAAATTAGAG
TAAACAATATTGCTGTTTAA

Downstream 100 bases:

>100_bases
AAACTAAAGAATTATAGCTAATGCAATCCCTACGTCATACCGCGATTCATTCGCGGTATCTCAGCCGCTAACAAGAGATC
CCGCTAACAAGCAGCGGGAT

Product: glutamyl-tRNA synthetase

Products: NA

Alternate protein names: Glutamate--tRNA ligase 2; GluRS 2 [H]

Number of amino acids: Translated: 440; Mature: 440

Protein sequence:

>440_residues
MLTRFAPSPTGYLHVGNVRTALVCWMYTRNQNGKFLLRFDDTDLQRSDVKYINDIIQDLKWIGINWDASFKQSERFERYN
EVFLQLIKEGHIYACYETREELDIKRKLQLKQGLPPVYDRSALLLTEQEKISYEQEGRRPHFRFKLDRNEVVKWNDEVKD
EINIATSSISDPVVKREDGIYTYMLPSVIDDVDFNVTHVVRGEDHVTNTAVQLQMIKALKAKIPTFAHLSLLHFDDSKIS
KRTGVLNIKSIKEDEIEPMALVSYLVKLGTSNPIEACACMQSLIDSFNIKKFSSASVQFSLSEIYKLNSKVLQQMPFEMV
QDRLNQIGVDSSEFWYFIRNNIERFSEVAKWWKICKSDIEPVILDKELIKIAFNALPQGDCNENTLSEWVKTIRQTVDIK
AKNLFTQLRSALTGTETGPELAKLLIFIGKENIIARLKEK

Sequences:

>Translated_440_residues
MLTRFAPSPTGYLHVGNVRTALVCWMYTRNQNGKFLLRFDDTDLQRSDVKYINDIIQDLKWIGINWDASFKQSERFERYN
EVFLQLIKEGHIYACYETREELDIKRKLQLKQGLPPVYDRSALLLTEQEKISYEQEGRRPHFRFKLDRNEVVKWNDEVKD
EINIATSSISDPVVKREDGIYTYMLPSVIDDVDFNVTHVVRGEDHVTNTAVQLQMIKALKAKIPTFAHLSLLHFDDSKIS
KRTGVLNIKSIKEDEIEPMALVSYLVKLGTSNPIEACACMQSLIDSFNIKKFSSASVQFSLSEIYKLNSKVLQQMPFEMV
QDRLNQIGVDSSEFWYFIRNNIERFSEVAKWWKICKSDIEPVILDKELIKIAFNALPQGDCNENTLSEWVKTIRQTVDIK
AKNLFTQLRSALTGTETGPELAKLLIFIGKENIIARLKEK
>Mature_440_residues
MLTRFAPSPTGYLHVGNVRTALVCWMYTRNQNGKFLLRFDDTDLQRSDVKYINDIIQDLKWIGINWDASFKQSERFERYN
EVFLQLIKEGHIYACYETREELDIKRKLQLKQGLPPVYDRSALLLTEQEKISYEQEGRRPHFRFKLDRNEVVKWNDEVKD
EINIATSSISDPVVKREDGIYTYMLPSVIDDVDFNVTHVVRGEDHVTNTAVQLQMIKALKAKIPTFAHLSLLHFDDSKIS
KRTGVLNIKSIKEDEIEPMALVSYLVKLGTSNPIEACACMQSLIDSFNIKKFSSASVQFSLSEIYKLNSKVLQQMPFEMV
QDRLNQIGVDSSEFWYFIRNNIERFSEVAKWWKICKSDIEPVILDKELIKIAFNALPQGDCNENTLSEWVKTIRQTVDIK
AKNLFTQLRSALTGTETGPELAKLLIFIGKENIIARLKEK

Specific function: Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction:glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu) [H]

COG id: COG0008

COG function: function code J; Glutamyl- and glutaminyl-tRNA synthetases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI134288884, Length=487, Percent_Identity=26.8993839835729, Blast_Score=164, Evalue=1e-40,
Organism=Homo sapiens, GI62241042, Length=242, Percent_Identity=26.4462809917355, Blast_Score=72, Evalue=1e-12,
Organism=Escherichia coli, GI1788743, Length=458, Percent_Identity=31.6593886462882, Blast_Score=243, Evalue=2e-65,
Organism=Escherichia coli, GI226510925, Length=274, Percent_Identity=27.007299270073, Blast_Score=101, Evalue=9e-23,
Organism=Escherichia coli, GI1786895, Length=141, Percent_Identity=30.4964539007092, Blast_Score=76, Evalue=4e-15,
Organism=Caenorhabditis elegans, GI71993394, Length=250, Percent_Identity=36, Blast_Score=153, Evalue=2e-37,
Organism=Saccharomyces cerevisiae, GI6324540, Length=327, Percent_Identity=32.1100917431193, Blast_Score=163, Evalue=5e-41,
Organism=Saccharomyces cerevisiae, GI6321192, Length=260, Percent_Identity=25.7692307692308, Blast_Score=80, Evalue=7e-16,
Organism=Saccharomyces cerevisiae, GI6324742, Length=127, Percent_Identity=34.6456692913386, Blast_Score=78, Evalue=2e-15,
Organism=Drosophila melanogaster, GI24665321, Length=481, Percent_Identity=31.1850311850312, Blast_Score=184, Evalue=1e-46,
Organism=Drosophila melanogaster, GI24649466, Length=243, Percent_Identity=26.3374485596708, Blast_Score=74, Evalue=1e-13,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008925
- InterPro:   IPR020751
- InterPro:   IPR001412
- InterPro:   IPR004527
- InterPro:   IPR000924
- InterPro:   IPR020061
- InterPro:   IPR020058
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00749 tRNA-synt_1c [H]

EC number: =6.1.1.17 [H]

Molecular weight: Translated: 50996; Mature: 50996

Theoretical pI: Translated: 7.15; Mature: 7.15

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLTRFAPSPTGYLHVGNVRTALVCWMYTRNQNGKFLLRFDDTDLQRSDVKYINDIIQDLK
CCCCCCCCCCCEEEECCHHEEEEEEEEEECCCCEEEEEECCCCCCHHHHHHHHHHHHHHE
WIGINWDASFKQSERFERYNEVFLQLIKEGHIYACYETREELDIKRKLQLKQGLPPVYDR
EEECCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEEECCHHHHHHHHHHHHHHCCCCCCCC
SALLLTEQEKISYEQEGRRPHFRFKLDRNEVVKWNDEVKDEINIATSSISDPVVKREDGI
CEEEEECHHHHCHHHHCCCCCEEEEECCCCEEECCCHHHHHHHHHHCCCCCCCCCCCCCE
YTYMLPSVIDDVDFNVTHVVRGEDHVTNTAVQLQMIKALKAKIPTFAHLSLLHFDDSKIS
EEEECHHHHCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHCCCCHHEEEEEEECCHHHH
KRTGVLNIKSIKEDEIEPMALVSYLVKLGTSNPIEACACMQSLIDSFNIKKFSSASVQFS
HHCCCEEECCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCCCEEEE
LSEIYKLNSKVLQQMPFEMVQDRLNQIGVDSSEFWYFIRNNIERFSEVAKWWKICKSDIE
HHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
PVILDKELIKIAFNALPQGDCNENTLSEWVKTIRQTVDIKAKNLFTQLRSALTGTETGPE
CEEEHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHCCCCCCHH
LAKLLIFIGKENIIARLKEK
HHHHHHHHCCCHHHHHHCCC
>Mature Secondary Structure
MLTRFAPSPTGYLHVGNVRTALVCWMYTRNQNGKFLLRFDDTDLQRSDVKYINDIIQDLK
CCCCCCCCCCCEEEECCHHEEEEEEEEEECCCCEEEEEECCCCCCHHHHHHHHHHHHHHE
WIGINWDASFKQSERFERYNEVFLQLIKEGHIYACYETREELDIKRKLQLKQGLPPVYDR
EEECCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEEECCHHHHHHHHHHHHHHCCCCCCCC
SALLLTEQEKISYEQEGRRPHFRFKLDRNEVVKWNDEVKDEINIATSSISDPVVKREDGI
CEEEEECHHHHCHHHHCCCCCEEEEECCCCEEECCCHHHHHHHHHHCCCCCCCCCCCCCE
YTYMLPSVIDDVDFNVTHVVRGEDHVTNTAVQLQMIKALKAKIPTFAHLSLLHFDDSKIS
EEEECHHHHCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHCCCCHHEEEEEEECCHHHH
KRTGVLNIKSIKEDEIEPMALVSYLVKLGTSNPIEACACMQSLIDSFNIKKFSSASVQFS
HHCCCEEECCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCCCEEEE
LSEIYKLNSKVLQQMPFEMVQDRLNQIGVDSSEFWYFIRNNIERFSEVAKWWKICKSDIE
HHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
PVILDKELIKIAFNALPQGDCNENTLSEWVKTIRQTVDIKAKNLFTQLRSALTGTETGPE
CEEEHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHCCCCCCHH
LAKLLIFIGKENIIARLKEK
HHHHHHHHCCCHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA