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The map label for this gene is greA [H]

Identifier: 225630273

GI number: 225630273

Start: 536755

End: 537249

Strand: Reverse

Name: greA [H]

Synonym: WRi_004910

Alternate gene names: 225630273

Gene position: 537249-536755 (Counterclockwise)

Preceding gene: 225630274

Following gene: 225630272

Centisome position: 37.16

GC content: 36.36

Gene sequence:

>495_bases
ATGGCTTCATCCATTATTAATAAATTTCCTATGACGAGGGAAGGTTTTGAGAATATGCAAGCCGAACTTGAAAAATTAAA
GGAAGAAAAACCTTCTGTGATACAGGCTATTTCTGATGCTCGTGATCAAGGTGATTTGTCTGAAAACGCAGAGTATCATG
CAGCACGGGAAAGGTTAGGTTTTATTGAAGGCCGTATAATGGAGATAGAAAGTAAGCTCTCACATGCGGAAGTAATAGAA
GTAAAAAATTTGTCTGGTGATTCAGTAATGTTTGGTGCAACTGTGACATTAAGCATGTTAAGTGATAATAACGGTGAAGT
GGAATATGTTTATAAGGTTGTAGGCGAATATGAAGCTGATGTTTCAAAGCAGTTAATATCTACTAGTTCACCACTTGGGA
GTGCCTTAATCGGCAAAAAAGTCGGTGAATATGTGGAAGTGACAGTGCCAAATGGAGAGAAATTGTATAAAATAGTTAAG
ATTGAATTTAAGTAA

Upstream 100 bases:

>100_bases
AAAAAAGTTGTCAAATGACATGAGTGATGATATAAAAGGTGATATTTTTAACATTGTGAGTAATTTCGTTACTCAATTTA
ATTGCGTTTAGGTGGTGATT

Downstream 100 bases:

>100_bases
ATTATTATGGTACAGGCCACTTATGCTTCAATGAGTCTACCAGGTATTTCTTCTGTGGAAGATGTATTAGAGGATGCTCG
TTCCGGTAAATTATTCATTT

Product: transcription elongation factor GreA

Products: NA

Alternate protein names: Transcript cleavage factor greA [H]

Number of amino acids: Translated: 164; Mature: 163

Protein sequence:

>164_residues
MASSIINKFPMTREGFENMQAELEKLKEEKPSVIQAISDARDQGDLSENAEYHAARERLGFIEGRIMEIESKLSHAEVIE
VKNLSGDSVMFGATVTLSMLSDNNGEVEYVYKVVGEYEADVSKQLISTSSPLGSALIGKKVGEYVEVTVPNGEKLYKIVK
IEFK

Sequences:

>Translated_164_residues
MASSIINKFPMTREGFENMQAELEKLKEEKPSVIQAISDARDQGDLSENAEYHAARERLGFIEGRIMEIESKLSHAEVIE
VKNLSGDSVMFGATVTLSMLSDNNGEVEYVYKVVGEYEADVSKQLISTSSPLGSALIGKKVGEYVEVTVPNGEKLYKIVK
IEFK
>Mature_163_residues
ASSIINKFPMTREGFENMQAELEKLKEEKPSVIQAISDARDQGDLSENAEYHAARERLGFIEGRIMEIESKLSHAEVIEV
KNLSGDSVMFGATVTLSMLSDNNGEVEYVYKVVGEYEADVSKQLISTSSPLGSALIGKKVGEYVEVTVPNGEKLYKIVKI
EFK

Specific function: Necessary for efficient RNA polymerase transcription elongation past template-encoded arresting sites. The arresting sites in DNA have the property of trapping a certain fraction of elongating RNA polymerases that pass through, resulting in locked ternary

COG id: COG0782

COG function: function code K; Transcription elongation factor

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the greA/greB family [H]

Homologues:

Organism=Escherichia coli, GI87082225, Length=158, Percent_Identity=47.4683544303797, Blast_Score=139, Evalue=8e-35,
Organism=Escherichia coli, GI87082261, Length=158, Percent_Identity=33.5443037974684, Blast_Score=84, Evalue=3e-18,

Paralogues:

None

Copy number: 259 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018151
- InterPro:   IPR006359
- InterPro:   IPR001437
- InterPro:   IPR022691 [H]

Pfam domain/function: PF01272 GreA_GreB; PF03449 GreA_GreB_N [H]

EC number: NA

Molecular weight: Translated: 18169; Mature: 18038

Theoretical pI: Translated: 4.54; Mature: 4.54

Prosite motif: PS00829 GREAB_1 ; PS00830 GREAB_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MASSIINKFPMTREGFENMQAELEKLKEEKPSVIQAISDARDQGDLSENAEYHAARERLG
CCCHHHHHCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHH
FIEGRIMEIESKLSHAEVIEVKNLSGDSVMFGATVTLSMLSDNNGEVEYVYKVVGEYEAD
HHHHHHHHHHHHCCHHHEEEEECCCCCEEEEHHEEEEEEECCCCCCHHHHHHHHHHHHHH
VSKQLISTSSPLGSALIGKKVGEYVEVTVPNGEKLYKIVKIEFK
HHHHHHHCCCCCHHHHHHHHCCCEEEEECCCCCEEEEEEEEEEC
>Mature Secondary Structure 
ASSIINKFPMTREGFENMQAELEKLKEEKPSVIQAISDARDQGDLSENAEYHAARERLG
CCHHHHHCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHH
FIEGRIMEIESKLSHAEVIEVKNLSGDSVMFGATVTLSMLSDNNGEVEYVYKVVGEYEAD
HHHHHHHHHHHHCCHHHEEEEECCCCCEEEEHHEEEEEEECCCCCCHHHHHHHHHHHHHH
VSKQLISTSSPLGSALIGKKVGEYVEVTVPNGEKLYKIVKIEFK
HHHHHHHCCCCCHHHHHHHHCCCEEEEECCCCCEEEEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA