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The map label for this gene is metG [H]

Identifier: 225630227

GI number: 225630227

Start: 467460

End: 469106

Strand: Reverse

Name: metG [H]

Synonym: WRi_004350

Alternate gene names: 225630227

Gene position: 469106-467460 (Counterclockwise)

Preceding gene: 225630228

Following gene: 225630225

Centisome position: 32.44

GC content: 39.95

Gene sequence:

>1647_bases
ATGGAACAATTCGAGAACTTTTACATCACCACGCCAGTATATTACGTAAACGATAAGCCGCATATTGGTCATGCATACAC
TTCTCTCTTGTGTGACGTGGTGGCAAGGTTCATGAAACTAGCTGGGAAAAATGTCAAATTTACCACCGGTACAGATGAAC
ATGGACAAAAAATAGAAAAAGCAGCTAAAGCAAAGGAAATGCAGCCAAAAGAATTTACAGATGATGTGAGCGTTTCATTT
AAAGAATTGGCTAAGTTCATGAATTTTGAGTATGACGATTTTATTCGCACTACGGAGGAACGTCACAAAAAAGCAGTTAT
AGCTTTATGGAATAGACTTGAAGAGAGAGGGCAAATATATTTGGATTCCTATTCGGGTTGGTATTCAGTTCGTGATGAAG
CATTTTATCAGGAATCAGAGCTGATAGATGGCAAAGCACCAACAGGCGCAGAAGTTCAGTGGATAAAAGAAGAGAGCTAC
TTTTTTCGCTTGTCAAACTGGCAAGACAAATTACTGGAACTCTATAAAAATCAGCCAAGTTTTATCTTTCCTGAGAGCAG
AAGAAATGAAGTGATATCGTTTGTAAGATCAGGGCTCATTGACCTCTCAATCTCTCGCACTAGTTTTAACTGGGGAATAA
AAGTACCAGGTAATGACAAACACGTAATCTATGTCTGGATAGATGCATTAACCAATTATCTTACATCAATAGGTTTCCCT
AGTACTGAAGGTGAGGAATATAAGAGGTTTTGGGCAGAACCAGAAACACAGATTCCAGTGTCAAGCACTGGAATGACACC
AAACCAAGGGAAAACGCAGATTCCAGTGTCAAGCACTGGAATGACGCCGAACCTAGCTGACAACTCTTTCAACGTTCATG
TAATCGGTAAAGACATATTGCGCTTTCATGCTGTATATTGGCCAGCGATTCTCCTTGCAGCAGACCTGCCACTGCCAAAA
CAAATTGCAGTTCATGGTTGGTGGTTGAACGAGGGAGAGAAAATATCCAAGTCCCTTGGTAATGTCATAGATCCAATTGG
CCTCGCTCAAGAGTTTGGTGTTGATCAGCTACGCTATTTTCTCCTCAGGGAAGCAAGCTTCGGCCAAGATGGCAACTTTA
GTAAGAAAAATATGATCAGCCGGATAAATTCAGAACTGGCAAACAACATAGGCAATTTAGTGCAAAGAACAATTTCATTT
TTACACAAGCAATGTTCTGGAATTGTACCAACAATCGATCAAATCCTACTTAAAGGTGAAGAAAGTCTTCCAGGTTGCAA
AGCTATACTCGATCAAGTGATGAATCACCTATCAAAGTATGAATTTAACCATATCATACTTCTAATCATCAACATCTCTT
CTGAAGCAAATGCCTACATAGACAAAAGTGCACCCTGGACACTAAGCAAAACTGACAGAGAGCGCGTGAATTTAGTAATT
TACAAGTTACTGGAATATATCAGGATAATAGGCATTTTATTGCAGCCAATCGTTCCCAAGTCAGCAGAGATGATACTAAA
TCAACTGCAAATTCCGAAAGAACAACGAGATTTAAAATCTCTGTGCAACGCATGCGTAAGCTTAGGCATTACGCTGCCTA
AACCTACGCCGGTTTTTTTGAGGGTTGATGCTAAAGAGGAGAAATAG

Upstream 100 bases:

>100_bases
GCTTTTTTCAAAAATTATGCTTAACCCAATCAATATTTATTTATTACTGCCTTTTTAATACTATAATTAACTCAGTTGAT
TTTTATCAAAATGCTAAACC

Downstream 100 bases:

>100_bases
TGTAAAAACTATTTCTCCTGGATGGGATTATCTTTAGTTAACAACGGTGCGCACCGCTGTCTACATATTCAAGTTAGGTG
GCAAGTGCCTTAGTTACTTT

Product: methionyl-tRNA synthetase

Products: NA

Alternate protein names: Methionine--tRNA ligase; MetRS [H]

Number of amino acids: Translated: 548; Mature: 548

Protein sequence:

>548_residues
MEQFENFYITTPVYYVNDKPHIGHAYTSLLCDVVARFMKLAGKNVKFTTGTDEHGQKIEKAAKAKEMQPKEFTDDVSVSF
KELAKFMNFEYDDFIRTTEERHKKAVIALWNRLEERGQIYLDSYSGWYSVRDEAFYQESELIDGKAPTGAEVQWIKEESY
FFRLSNWQDKLLELYKNQPSFIFPESRRNEVISFVRSGLIDLSISRTSFNWGIKVPGNDKHVIYVWIDALTNYLTSIGFP
STEGEEYKRFWAEPETQIPVSSTGMTPNQGKTQIPVSSTGMTPNLADNSFNVHVIGKDILRFHAVYWPAILLAADLPLPK
QIAVHGWWLNEGEKISKSLGNVIDPIGLAQEFGVDQLRYFLLREASFGQDGNFSKKNMISRINSELANNIGNLVQRTISF
LHKQCSGIVPTIDQILLKGEESLPGCKAILDQVMNHLSKYEFNHIILLIINISSEANAYIDKSAPWTLSKTDRERVNLVI
YKLLEYIRIIGILLQPIVPKSAEMILNQLQIPKEQRDLKSLCNACVSLGITLPKPTPVFLRVDAKEEK

Sequences:

>Translated_548_residues
MEQFENFYITTPVYYVNDKPHIGHAYTSLLCDVVARFMKLAGKNVKFTTGTDEHGQKIEKAAKAKEMQPKEFTDDVSVSF
KELAKFMNFEYDDFIRTTEERHKKAVIALWNRLEERGQIYLDSYSGWYSVRDEAFYQESELIDGKAPTGAEVQWIKEESY
FFRLSNWQDKLLELYKNQPSFIFPESRRNEVISFVRSGLIDLSISRTSFNWGIKVPGNDKHVIYVWIDALTNYLTSIGFP
STEGEEYKRFWAEPETQIPVSSTGMTPNQGKTQIPVSSTGMTPNLADNSFNVHVIGKDILRFHAVYWPAILLAADLPLPK
QIAVHGWWLNEGEKISKSLGNVIDPIGLAQEFGVDQLRYFLLREASFGQDGNFSKKNMISRINSELANNIGNLVQRTISF
LHKQCSGIVPTIDQILLKGEESLPGCKAILDQVMNHLSKYEFNHIILLIINISSEANAYIDKSAPWTLSKTDRERVNLVI
YKLLEYIRIIGILLQPIVPKSAEMILNQLQIPKEQRDLKSLCNACVSLGITLPKPTPVFLRVDAKEEK
>Mature_548_residues
MEQFENFYITTPVYYVNDKPHIGHAYTSLLCDVVARFMKLAGKNVKFTTGTDEHGQKIEKAAKAKEMQPKEFTDDVSVSF
KELAKFMNFEYDDFIRTTEERHKKAVIALWNRLEERGQIYLDSYSGWYSVRDEAFYQESELIDGKAPTGAEVQWIKEESY
FFRLSNWQDKLLELYKNQPSFIFPESRRNEVISFVRSGLIDLSISRTSFNWGIKVPGNDKHVIYVWIDALTNYLTSIGFP
STEGEEYKRFWAEPETQIPVSSTGMTPNQGKTQIPVSSTGMTPNLADNSFNVHVIGKDILRFHAVYWPAILLAADLPLPK
QIAVHGWWLNEGEKISKSLGNVIDPIGLAQEFGVDQLRYFLLREASFGQDGNFSKKNMISRINSELANNIGNLVQRTISF
LHKQCSGIVPTIDQILLKGEESLPGCKAILDQVMNHLSKYEFNHIILLIINISSEANAYIDKSAPWTLSKTDRERVNLVI
YKLLEYIRIIGILLQPIVPKSAEMILNQLQIPKEQRDLKSLCNACVSLGITLPKPTPVFLRVDAKEEK

Specific function: Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation [H]

COG id: COG0143

COG function: function code J; Methionyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family. MetG type 2B subfamily [H]

Homologues:

Organism=Homo sapiens, GI24308436, Length=552, Percent_Identity=35.8695652173913, Blast_Score=345, Evalue=5e-95,
Organism=Homo sapiens, GI14043022, Length=550, Percent_Identity=25.6363636363636, Blast_Score=148, Evalue=1e-35,
Organism=Escherichia coli, GI1788432, Length=540, Percent_Identity=23.7037037037037, Blast_Score=153, Evalue=2e-38,
Organism=Caenorhabditis elegans, GI212645096, Length=540, Percent_Identity=35.3703703703704, Blast_Score=320, Evalue=9e-88,
Organism=Caenorhabditis elegans, GI71992781, Length=434, Percent_Identity=32.9493087557604, Blast_Score=220, Evalue=2e-57,
Organism=Caenorhabditis elegans, GI17540794, Length=594, Percent_Identity=24.4107744107744, Blast_Score=145, Evalue=4e-35,
Organism=Saccharomyces cerevisiae, GI6321610, Length=548, Percent_Identity=35.5839416058394, Blast_Score=322, Evalue=9e-89,
Organism=Saccharomyces cerevisiae, GI6321704, Length=588, Percent_Identity=23.6394557823129, Blast_Score=117, Evalue=4e-27,
Organism=Drosophila melanogaster, GI21358483, Length=534, Percent_Identity=36.3295880149813, Blast_Score=334, Evalue=9e-92,
Organism=Drosophila melanogaster, GI24655311, Length=596, Percent_Identity=25.503355704698, Blast_Score=147, Evalue=2e-35,

Paralogues:

None

Copy number: 871 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015413
- InterPro:   IPR014758
- InterPro:   IPR014729
- InterPro:   IPR009080
- InterPro:   IPR013155 [H]

Pfam domain/function: PF08264 Anticodon_1; PF09334 tRNA-synt_1g [H]

EC number: =6.1.1.10 [H]

Molecular weight: Translated: 62584; Mature: 62584

Theoretical pI: Translated: 6.60; Mature: 6.60

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEQFENFYITTPVYYVNDKPHIGHAYTSLLCDVVARFMKLAGKNVKFTTGTDEHGQKIEK
CCCCCCEEEECCEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEECCCCHHHHHHHH
AAKAKEMQPKEFTDDVSVSFKELAKFMNFEYDDFIRTTEERHKKAVIALWNRLEERGQIY
HHHHHCCCCCCCCCHHCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCEE
LDSYSGWYSVRDEAFYQESELIDGKAPTGAEVQWIKEESYFFRLSNWQDKLLELYKNQPS
EECCCCCEEHHHHHHHHHHHCCCCCCCCCCCEEEEECCCEEEEECCHHHHHHHHHCCCCC
FIFPESRRNEVISFVRSGLIDLSISRTSFNWGIKVPGNDKHVIYVWIDALTNYLTSIGFP
EECCCHHHHHHHHHHHCCCEEEEEEEEECCCEEEECCCCCEEEEEEHHHHHHHHHHCCCC
STEGEEYKRFWAEPETQIPVSSTGMTPNQGKTQIPVSSTGMTPNLADNSFNVHVIGKDIL
CCCCHHHHHHHCCCCCCCCCCCCCCCCCCCCEECCCCCCCCCCCCCCCCEEEEEECHHHH
RFHAVYWPAILLAADLPLPKQIAVHGWWLNEGEKISKSLGNVIDPIGLAQEFGVDQLRYF
HHHHHHHHHHHHHCCCCCCCEEEEEEEECCCCHHHHHHHHHHHCCCHHHHHHCHHHHHHH
LLREASFGQDGNFSKKNMISRINSELANNIGNLVQRTISFLHKQCSGIVPTIDQILLKGE
HHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCC
ESLPGCKAILDQVMNHLSKYEFNHIILLIINISSEANAYIDKSAPWTLSKTDRERVNLVI
CCCCHHHHHHHHHHHHHHHCCCCEEEEEEEEECCCCCEEECCCCCEECCCCCHHHHHHHH
YKLLEYIRIIGILLQPIVPKSAEMILNQLQIPKEQRDLKSLCNACVSLGITLPKPTPVFL
HHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCCCCEEE
RVDAKEEK
EECCCCCC
>Mature Secondary Structure
MEQFENFYITTPVYYVNDKPHIGHAYTSLLCDVVARFMKLAGKNVKFTTGTDEHGQKIEK
CCCCCCEEEECCEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEECCCCHHHHHHHH
AAKAKEMQPKEFTDDVSVSFKELAKFMNFEYDDFIRTTEERHKKAVIALWNRLEERGQIY
HHHHHCCCCCCCCCHHCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCEE
LDSYSGWYSVRDEAFYQESELIDGKAPTGAEVQWIKEESYFFRLSNWQDKLLELYKNQPS
EECCCCCEEHHHHHHHHHHHCCCCCCCCCCCEEEEECCCEEEEECCHHHHHHHHHCCCCC
FIFPESRRNEVISFVRSGLIDLSISRTSFNWGIKVPGNDKHVIYVWIDALTNYLTSIGFP
EECCCHHHHHHHHHHHCCCEEEEEEEEECCCEEEECCCCCEEEEEEHHHHHHHHHHCCCC
STEGEEYKRFWAEPETQIPVSSTGMTPNQGKTQIPVSSTGMTPNLADNSFNVHVIGKDIL
CCCCHHHHHHHCCCCCCCCCCCCCCCCCCCCEECCCCCCCCCCCCCCCCEEEEEECHHHH
RFHAVYWPAILLAADLPLPKQIAVHGWWLNEGEKISKSLGNVIDPIGLAQEFGVDQLRYF
HHHHHHHHHHHHHCCCCCCCEEEEEEEECCCCHHHHHHHHHHHCCCHHHHHHCHHHHHHH
LLREASFGQDGNFSKKNMISRINSELANNIGNLVQRTISFLHKQCSGIVPTIDQILLKGE
HHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCC
ESLPGCKAILDQVMNHLSKYEFNHIILLIINISSEANAYIDKSAPWTLSKTDRERVNLVI
CCCCHHHHHHHHHHHHHHHCCCCEEEEEEEEECCCCCEEECCCCCEECCCCCHHHHHHHH
YKLLEYIRIIGILLQPIVPKSAEMILNQLQIPKEQRDLKSLCNACVSLGITLPKPTPVFL
HHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCCCCEEE
RVDAKEEK
EECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA