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The map label for this gene is gltP [H]

Identifier: 225630049

GI number: 225630049

Start: 222501

End: 223685

Strand: Reverse

Name: gltP [H]

Synonym: WRi_002180

Alternate gene names: 225630049

Gene position: 223685-222501 (Counterclockwise)

Preceding gene: 225630050

Following gene: 225630048

Centisome position: 15.47

GC content: 33.76

Gene sequence:

>1185_bases
ATGCACAAGTTTGCAATACTTTTTTCTATACTGCTTGCAATAGTTGCTTACTACCTAAACAATGAAATCATATTTGAAAT
AGCAAAGCTATTTAGTGATATCTTTATCAGCTTGCTCAAATTAATAAGCTTACCTCTGGTTTTTTTATCCATAGTTTCCA
CAATTTCAGGGCTCAAGGACTCAATTGAAATCAAAGTCTTAGTTAAGAAAACACTATTTTATACTCTGCTTACAACTATT
ATAGCTGCGTTTGTTGCATTATCGTTTTATTTGTTAATAGATCCAGCAAGAAAAAATTTCATAAGTAATACAATAGGGAG
TATTAACAGCAATAACCACAGTTATTTTTCATATTTAAAATCACTCATTCCTTCTAATTTTGTGCAAGTTTTTCTTGAAA
ATAATATGATTGGTAGCATATTGATCGCTTTTTTGATGGGTGGAGCTATTGTTTCACTTTCAAAAGAAAAGCAGGATATA
TTATATCAAGTATTCTCTGCACTTTTTGATACGCTACTTAAAATTGCTCAAGGGTTATTAAAATTTATTCCGCTTGCTGT
GTGGTCCTTTATCACATGTTTTTTGCACGAATTAAAAGGCGGTAGCGAGTTCTACAGTCTTTTTTGGTATTTTGTTTGCA
TAATGTCTGCAAATTTTGTACAGGCATTTCTGATTTTACCGCTACTTATGTGGTATAAAGGCATATCACCAATTCAAACG
CTAAGGGGAGTTATGCCGGCACTCACACTTGCGTTTTTTTCTAAATCATCCAGTGCAACGCTGCCCACAACCATAGACTG
TGTGCAGAACAAGCTAAAGGTGCCAAAGAAATTGTCGTCTTTTATTTTACCAATATGCACAACAATTAATATGAATGCAT
GTGCTGCATTTATACTAATCACTGTAATGTTTGTTGCAGAAATGAACGGATACGCATTTTCCTTGAGCGAAATGTTGATA
TGGATTTTTTTAGCTACAGGGGCTGCAATTGGTAACGCTGGAGTGCCGATGGGGTGCTATTTTATGGCAACCAGTTATCT
CGTATCGATGAATGTTCCTTTGCATATTATGGGACTGATTTTGCCTATTTATACAATCATCGATATGTTTGAAACTGCAA
TAAATGTGTGGTCTGATATTTGCATAACTCAGATAATTAATAAAGAATGTAATAGTGAAACTTAA

Upstream 100 bases:

>100_bases
TCCTCTTATCTTTCCCTAATCTGTAAAGTTATTTTGTCTATCAATCTGATTCCAAAACAGCAAAATTATTGTATAATGTT
ACTTTGAAGTTTTATTTTCA

Downstream 100 bases:

>100_bases
AAAATGATATTAGACGAAAATAACCTAATAATAAAAGAATTTAAAGAAGCAGAAGCAATTTTGCATGGGCATTTTATTTT
ATCTTCTGGATTGCATAGCA

Product: Na+/H+-dicarboxylate symporter family protein

Products: L-aspartate [Cytoplasm]; Proton [Cytoplasm]; L-glutamate [Cytoplasm] [C]

Alternate protein names: Glutamate-aspartate carrier protein [H]

Number of amino acids: Translated: 394; Mature: 394

Protein sequence:

>394_residues
MHKFAILFSILLAIVAYYLNNEIIFEIAKLFSDIFISLLKLISLPLVFLSIVSTISGLKDSIEIKVLVKKTLFYTLLTTI
IAAFVALSFYLLIDPARKNFISNTIGSINSNNHSYFSYLKSLIPSNFVQVFLENNMIGSILIAFLMGGAIVSLSKEKQDI
LYQVFSALFDTLLKIAQGLLKFIPLAVWSFITCFLHELKGGSEFYSLFWYFVCIMSANFVQAFLILPLLMWYKGISPIQT
LRGVMPALTLAFFSKSSSATLPTTIDCVQNKLKVPKKLSSFILPICTTINMNACAAFILITVMFVAEMNGYAFSLSEMLI
WIFLATGAAIGNAGVPMGCYFMATSYLVSMNVPLHIMGLILPIYTIIDMFETAINVWSDICITQIINKECNSET

Sequences:

>Translated_394_residues
MHKFAILFSILLAIVAYYLNNEIIFEIAKLFSDIFISLLKLISLPLVFLSIVSTISGLKDSIEIKVLVKKTLFYTLLTTI
IAAFVALSFYLLIDPARKNFISNTIGSINSNNHSYFSYLKSLIPSNFVQVFLENNMIGSILIAFLMGGAIVSLSKEKQDI
LYQVFSALFDTLLKIAQGLLKFIPLAVWSFITCFLHELKGGSEFYSLFWYFVCIMSANFVQAFLILPLLMWYKGISPIQT
LRGVMPALTLAFFSKSSSATLPTTIDCVQNKLKVPKKLSSFILPICTTINMNACAAFILITVMFVAEMNGYAFSLSEMLI
WIFLATGAAIGNAGVPMGCYFMATSYLVSMNVPLHIMGLILPIYTIIDMFETAINVWSDICITQIINKECNSET
>Mature_394_residues
MHKFAILFSILLAIVAYYLNNEIIFEIAKLFSDIFISLLKLISLPLVFLSIVSTISGLKDSIEIKVLVKKTLFYTLLTTI
IAAFVALSFYLLIDPARKNFISNTIGSINSNNHSYFSYLKSLIPSNFVQVFLENNMIGSILIAFLMGGAIVSLSKEKQDI
LYQVFSALFDTLLKIAQGLLKFIPLAVWSFITCFLHELKGGSEFYSLFWYFVCIMSANFVQAFLILPLLMWYKGISPIQT
LRGVMPALTLAFFSKSSSATLPTTIDCVQNKLKVPKKLSSFILPICTTINMNACAAFILITVMFVAEMNGYAFSLSEMLI
WIFLATGAAIGNAGVPMGCYFMATSYLVSMNVPLHIMGLILPIYTIIDMFETAINVWSDICITQIINKECNSET

Specific function: This carrier protein is part of the Na(+)-independent, binding-protein-independent glutamate-aspartate transport system [H]

COG id: COG1301

COG function: function code C; Na+/H+-dicarboxylate symporters

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sodium:dicarboxylate (SDF) symporter (TC 2.A.23) family [H]

Homologues:

Organism=Homo sapiens, GI66773030, Length=261, Percent_Identity=30.2681992337165, Blast_Score=118, Evalue=1e-26,
Organism=Homo sapiens, GI21314632, Length=391, Percent_Identity=25.8312020460358, Blast_Score=116, Evalue=4e-26,
Organism=Homo sapiens, GI223468564, Length=247, Percent_Identity=27.5303643724696, Blast_Score=106, Evalue=4e-23,
Organism=Homo sapiens, GI223468566, Length=247, Percent_Identity=27.5303643724696, Blast_Score=105, Evalue=5e-23,
Organism=Homo sapiens, GI4827012, Length=254, Percent_Identity=25.9842519685039, Blast_Score=102, Evalue=4e-22,
Organism=Homo sapiens, GI5032093, Length=247, Percent_Identity=27.5303643724696, Blast_Score=102, Evalue=7e-22,
Organism=Homo sapiens, GI194239697, Length=226, Percent_Identity=28.7610619469027, Blast_Score=101, Evalue=1e-21,
Organism=Homo sapiens, GI169790839, Length=238, Percent_Identity=26.890756302521, Blast_Score=99, Evalue=6e-21,
Organism=Homo sapiens, GI40254478, Length=406, Percent_Identity=21.9211822660099, Blast_Score=86, Evalue=4e-17,
Organism=Homo sapiens, GI301601644, Length=122, Percent_Identity=34.4262295081967, Blast_Score=86, Evalue=6e-17,
Organism=Homo sapiens, GI262359914, Length=178, Percent_Identity=25.8426966292135, Blast_Score=74, Evalue=3e-13,
Organism=Escherichia coli, GI1790514, Length=399, Percent_Identity=24.3107769423559, Blast_Score=117, Evalue=1e-27,
Organism=Escherichia coli, GI1788024, Length=395, Percent_Identity=26.8354430379747, Blast_Score=94, Evalue=2e-20,
Organism=Escherichia coli, GI1789473, Length=385, Percent_Identity=23.8961038961039, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1789947, Length=400, Percent_Identity=20.25, Blast_Score=69, Evalue=5e-13,
Organism=Caenorhabditis elegans, GI71996953, Length=414, Percent_Identity=22.463768115942, Blast_Score=124, Evalue=7e-29,
Organism=Caenorhabditis elegans, GI193206505, Length=420, Percent_Identity=23.8095238095238, Blast_Score=122, Evalue=2e-28,
Organism=Caenorhabditis elegans, GI71983099, Length=398, Percent_Identity=23.1155778894472, Blast_Score=114, Evalue=1e-25,
Organism=Caenorhabditis elegans, GI71983106, Length=398, Percent_Identity=23.1155778894472, Blast_Score=113, Evalue=2e-25,
Organism=Caenorhabditis elegans, GI193206654, Length=400, Percent_Identity=25.75, Blast_Score=110, Evalue=1e-24,
Organism=Caenorhabditis elegans, GI17537407, Length=385, Percent_Identity=24.9350649350649, Blast_Score=104, Evalue=8e-23,
Organism=Caenorhabditis elegans, GI17541374, Length=394, Percent_Identity=22.8426395939086, Blast_Score=92, Evalue=3e-19,
Organism=Drosophila melanogaster, GI24583025, Length=394, Percent_Identity=24.8730964467005, Blast_Score=112, Evalue=4e-25,
Organism=Drosophila melanogaster, GI17137668, Length=394, Percent_Identity=24.8730964467005, Blast_Score=112, Evalue=4e-25,
Organism=Drosophila melanogaster, GI24583023, Length=394, Percent_Identity=24.8730964467005, Blast_Score=112, Evalue=4e-25,
Organism=Drosophila melanogaster, GI281360481, Length=420, Percent_Identity=20.7142857142857, Blast_Score=72, Evalue=6e-13,
Organism=Drosophila melanogaster, GI281360483, Length=420, Percent_Identity=20.7142857142857, Blast_Score=72, Evalue=8e-13,
Organism=Drosophila melanogaster, GI17137666, Length=420, Percent_Identity=20.7142857142857, Blast_Score=72, Evalue=8e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001991
- InterPro:   IPR018107 [H]

Pfam domain/function: PF00375 SDF [H]

EC number: NA

Molecular weight: Translated: 43959; Mature: 43959

Theoretical pI: Translated: 8.18; Mature: 8.18

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure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HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: L-aspartate [Periplasm]; Proton [Periplasm]; L-glutamate [Periplasm] [C]

Specific reaction: Proton [Periplasm] + L-aspartate [Periplasm] = Proton [Cytoplasm] + L-aspartate [Cytoplasm] Proton [Periplasm] + L-glutamate [Periplasm] = Proton [Cytoplasm] + L-glutamate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1971622; 1551855; 8265357; 9278503 [H]