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The map label for this gene is pyrC [H]

Identifier: 225630050

GI number: 225630050

Start: 223884

End: 225209

Strand: Reverse

Name: pyrC [H]

Synonym: WRi_002190

Alternate gene names: 225630050

Gene position: 225209-223884 (Counterclockwise)

Preceding gene: 225630051

Following gene: 225630049

Centisome position: 15.58

GC content: 38.84

Gene sequence:

>1326_bases
ATGACACAGACTTGGAATTTGTTACAAACAGGGCAAAAGGAAGGCTATAAAATTGCGTATACTAATGCTCGTATTATCGA
CCCAGAAACTAAACTTGATATAAAAGGTTCGCTATTGACTGAAGGGGATAAAATTGTTGATTTTGGCGAATCATTATTTT
CAACAAGTGGAGTTGATGAAACAATAGACTGTAAAGGGCTTGTTCTAATGCCAGGCCTTATTGACATTCACGTACATTTT
CGTGAACCAGGGCAGGAACATAAAGAAACTATATATACAGGTAGCAAATCTGCAGCTGCAGGAGGCGTTACAACTGTAGT
TTGTCAGCCAAACACTATTCCAGCAATTGATAGCGTTGTTTTGGCTAAATATTTGAAATATAGAGCACTTGAAACTTCAC
ATGTAAACGTTGAATTTTACGCTAAAATCACCACTTTGGAAGAAAAGTTGACTGAAATGGCACTTTTAAAGGAAGCAGGT
GCGGTTGGGTTCACTGATGATGGCATGCCAGTTATGAACCCAATGATTATGAGACAGGCGCTGCTTTATTCAAGCATGCT
GGGTGTTCCTATTGCTCAACATGCAGAAGATTTAAATTTATCAGCAGGTGGTGCAATCAACGAAGGTAAAATTTCTGAAG
AATTGGGAGTAAAGGGGATCTTGAGTGCGTCAGAATCGGTTATGGTAAATCGTGATATACTGCTAATGAAGGATATGGAA
AATGTGCACTACCATATTCTACATGTCTCTTCAAAAGATTCACTTGATGCTATTAAACGTGCAAAAGATTTGGGATTAAA
TGTTACGTGCGAAGTAACCCCTCATCACTTCACTTTGACTGAAGATATAGTAAAACAACATGGAGCAATTGCAAAAATGA
ATCCGCCGCTTCGCACTGAAGAAGATCGTTTAGCTATGGTTGAAGGTTTAAAGACAGGTGTGATTGATTGCATTGCAACT
GATCACGCTCCGCATGATCGTAGTTCTAAAGATCTACCACTTGAAAATGCTGCATTTGGTATTGTTGGTCTTGAAACAAT
GCTGCCCCTTTCACTTGAACTCTATCACAGTGGGCAAATGGGTCTACTTGATGTGCTTGCAAAATTGACATATAAGCCTG
CAGATATAATACACATACCACGTGGTCGTATACAAAAAAACCTTGCTGCAGACTTAATTTTAGTTGACCTCAATCATGAA
TGGGAAATTAAAACTGACAACTTTGCTAGTAAATCAAAGAATTCTCCTTTCGATGGTCGCAAAGTAAAAGGACACGTAGT
ACGTACTGTTGTGTCCGGCAAAACTATATACTCGCAGAAGAGTTAG

Upstream 100 bases:

>100_bases
GGATAATTTTAATTCAAGACAGCTCTTAGTCTGTGTTTTAATTTGGTTGTGTTTTATTAAAAAAACTTTAGATTTATTGC
ATAATCGATACTCAAATATA

Downstream 100 bases:

>100_bases
TTATCATATCTTATCTATTCCAATTTGTTATCACCAGAATGGGCTAAGTGCACCTAAATTTAAATTAAGAAATCTTACAC
TGTCTCCGAATAAACACATC

Product: dihydroorotase

Products: NA

Alternate protein names: DHOase [H]

Number of amino acids: Translated: 441; Mature: 440

Protein sequence:

>441_residues
MTQTWNLLQTGQKEGYKIAYTNARIIDPETKLDIKGSLLTEGDKIVDFGESLFSTSGVDETIDCKGLVLMPGLIDIHVHF
REPGQEHKETIYTGSKSAAAGGVTTVVCQPNTIPAIDSVVLAKYLKYRALETSHVNVEFYAKITTLEEKLTEMALLKEAG
AVGFTDDGMPVMNPMIMRQALLYSSMLGVPIAQHAEDLNLSAGGAINEGKISEELGVKGILSASESVMVNRDILLMKDME
NVHYHILHVSSKDSLDAIKRAKDLGLNVTCEVTPHHFTLTEDIVKQHGAIAKMNPPLRTEEDRLAMVEGLKTGVIDCIAT
DHAPHDRSSKDLPLENAAFGIVGLETMLPLSLELYHSGQMGLLDVLAKLTYKPADIIHIPRGRIQKNLAADLILVDLNHE
WEIKTDNFASKSKNSPFDGRKVKGHVVRTVVSGKTIYSQKS

Sequences:

>Translated_441_residues
MTQTWNLLQTGQKEGYKIAYTNARIIDPETKLDIKGSLLTEGDKIVDFGESLFSTSGVDETIDCKGLVLMPGLIDIHVHF
REPGQEHKETIYTGSKSAAAGGVTTVVCQPNTIPAIDSVVLAKYLKYRALETSHVNVEFYAKITTLEEKLTEMALLKEAG
AVGFTDDGMPVMNPMIMRQALLYSSMLGVPIAQHAEDLNLSAGGAINEGKISEELGVKGILSASESVMVNRDILLMKDME
NVHYHILHVSSKDSLDAIKRAKDLGLNVTCEVTPHHFTLTEDIVKQHGAIAKMNPPLRTEEDRLAMVEGLKTGVIDCIAT
DHAPHDRSSKDLPLENAAFGIVGLETMLPLSLELYHSGQMGLLDVLAKLTYKPADIIHIPRGRIQKNLAADLILVDLNHE
WEIKTDNFASKSKNSPFDGRKVKGHVVRTVVSGKTIYSQKS
>Mature_440_residues
TQTWNLLQTGQKEGYKIAYTNARIIDPETKLDIKGSLLTEGDKIVDFGESLFSTSGVDETIDCKGLVLMPGLIDIHVHFR
EPGQEHKETIYTGSKSAAAGGVTTVVCQPNTIPAIDSVVLAKYLKYRALETSHVNVEFYAKITTLEEKLTEMALLKEAGA
VGFTDDGMPVMNPMIMRQALLYSSMLGVPIAQHAEDLNLSAGGAINEGKISEELGVKGILSASESVMVNRDILLMKDMEN
VHYHILHVSSKDSLDAIKRAKDLGLNVTCEVTPHHFTLTEDIVKQHGAIAKMNPPLRTEEDRLAMVEGLKTGVIDCIATD
HAPHDRSSKDLPLENAAFGIVGLETMLPLSLELYHSGQMGLLDVLAKLTYKPADIIHIPRGRIQKNLAADLILVDLNHEW
EIKTDNFASKSKNSPFDGRKVKGHVVRTVVSGKTIYSQKS

Specific function: Involved In The Anaerobic Utilization Of Allantoin. [C]

COG id: COG0044

COG function: function code F; Dihydroorotase and related cyclic amidohydrolases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DHOase family. Type 2 subfamily [H]

Homologues:

Organism=Homo sapiens, GI18105007, Length=387, Percent_Identity=33.3333333333333, Blast_Score=172, Evalue=5e-43,
Organism=Homo sapiens, GI19923821, Length=431, Percent_Identity=23.8979118329466, Blast_Score=67, Evalue=4e-11,
Organism=Escherichia coli, GI1786722, Length=445, Percent_Identity=27.6404494382022, Blast_Score=170, Evalue=1e-43,
Organism=Caenorhabditis elegans, GI193204318, Length=385, Percent_Identity=33.5064935064935, Blast_Score=176, Evalue=2e-44,
Organism=Saccharomyces cerevisiae, GI6322218, Length=389, Percent_Identity=26.4781491002571, Blast_Score=115, Evalue=1e-26,
Organism=Drosophila melanogaster, GI24642586, Length=383, Percent_Identity=30.8093994778068, Blast_Score=170, Evalue=1e-42,
Organism=Drosophila melanogaster, GI18859883, Length=436, Percent_Identity=27.5229357798165, Blast_Score=98, Evalue=1e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006680
- InterPro:   IPR004722
- InterPro:   IPR002195
- InterPro:   IPR011059 [H]

Pfam domain/function: PF01979 Amidohydro_1 [H]

EC number: =3.5.2.3 [H]

Molecular weight: Translated: 48318; Mature: 48187

Theoretical pI: Translated: 6.17; Mature: 6.17

Prosite motif: PS00482 DIHYDROOROTASE_1 ; PS00483 DIHYDROOROTASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTQTWNLLQTGQKEGYKIAYTNARIIDPETKLDIKGSLLTEGDKIVDFGESLFSTSGVDE
CCCCCCHHHCCCCCCEEEEEECCEEECCCCCEEECCCEECCCCHHHHHHHHHHHCCCCCC
TIDCKGLVLMPGLIDIHVHFREPGQEHKETIYTGSKSAAAGGVTTVVCQPNTIPAIDSVV
CCCCCCEEEECCEEEEEEEECCCCHHHHHHEECCCCCCCCCCEEEEEECCCCCCHHHHHH
LAKYLKYRALETSHVNVEFYAKITTLEEKLTEMALLKEAGAVGFTDDGMPVMNPMIMRQA
HHHHHHHHHCCCCCCCEEEEEEEHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHH
LLYSSMLGVPIAQHAEDLNLSAGGAINEGKISEELGVKGILSASESVMVNRDILLMKDME
HHHHHHHCCCHHHHHCCCCCCCCCCCCCCCCHHHHCCCEEECCCCCEEECCCEEEEECCC
NVHYHILHVSSKDSLDAIKRAKDLGLNVTCEVTPHHFTLTEDIVKQHGAIAKMNPPLRTE
CCEEEEEEECCCCHHHHHHHHHHCCCEEEEEECCCEEECHHHHHHHCCCEEECCCCCCCC
EDRLAMVEGLKTGVIDCIATDHAPHDRSSKDLPLENAAFGIVGLETMLPLSLELYHSGQM
CHHHHHHHHHHHCCEEEEECCCCCCCCCCCCCCCCCCCEEEEEHHHHCCEEEEEEECCCC
GLLDVLAKLTYKPADIIHIPRGRIQKNLAADLILVDLNHEWEIKTDNFASKSKNSPFDGR
CHHHHHHHHCCCCCCEEECCCHHHHHCCCEEEEEEECCCCEEEECCCCCCCCCCCCCCCC
KVKGHVVRTVVSGKTIYSQKS
CCCCEEEEEEECCCEEECCCC
>Mature Secondary Structure 
TQTWNLLQTGQKEGYKIAYTNARIIDPETKLDIKGSLLTEGDKIVDFGESLFSTSGVDE
CCCCCHHHCCCCCCEEEEEECCEEECCCCCEEECCCEECCCCHHHHHHHHHHHCCCCCC
TIDCKGLVLMPGLIDIHVHFREPGQEHKETIYTGSKSAAAGGVTTVVCQPNTIPAIDSVV
CCCCCCEEEECCEEEEEEEECCCCHHHHHHEECCCCCCCCCCEEEEEECCCCCCHHHHHH
LAKYLKYRALETSHVNVEFYAKITTLEEKLTEMALLKEAGAVGFTDDGMPVMNPMIMRQA
HHHHHHHHHCCCCCCCEEEEEEEHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHH
LLYSSMLGVPIAQHAEDLNLSAGGAINEGKISEELGVKGILSASESVMVNRDILLMKDME
HHHHHHHCCCHHHHHCCCCCCCCCCCCCCCCHHHHCCCEEECCCCCEEECCCEEEEECCC
NVHYHILHVSSKDSLDAIKRAKDLGLNVTCEVTPHHFTLTEDIVKQHGAIAKMNPPLRTE
CCEEEEEEECCCCHHHHHHHHHHCCCEEEEEECCCEEECHHHHHHHCCCEEECCCCCCCC
EDRLAMVEGLKTGVIDCIATDHAPHDRSSKDLPLENAAFGIVGLETMLPLSLELYHSGQM
CHHHHHHHHHHHCCEEEEECCCCCCCCCCCCCCCCCCCEEEEEHHHHCCEEEEEEECCCC
GLLDVLAKLTYKPADIIHIPRGRIQKNLAADLILVDLNHEWEIKTDNFASKSKNSPFDGR
CHHHHHHHHCCCCCCEEECCCHHHHHCCCEEEEEEECCCCEEEECCCCCCCCCCCCCCCC
KVKGHVVRTVVSGKTIYSQKS
CCCCEEEEEEECCCEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA