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The map label for this gene is pyrE [H]

Identifier: 225630048

GI number: 225630048

Start: 221901

End: 222497

Strand: Reverse

Name: pyrE [H]

Synonym: WRi_002170

Alternate gene names: 225630048

Gene position: 222497-221901 (Counterclockwise)

Preceding gene: 225630049

Following gene: 225630047

Centisome position: 15.39

GC content: 34.0

Gene sequence:

>597_bases
ATGATATTAGACGAAAATAACCTAATAATAAAAGAATTTAAAGAAGCAGAAGCAATTTTGCATGGGCATTTTATTTTATC
TTCTGGATTGCATAGCAATACATATATACAATGCGCTAAAATTTTTGAAAATCCAAGCAGAGCCATGAAAGTTTGCGCAT
TATTAGCAGATAAAATAAAAAAAGAATTAAATAGCTCCATAGATTTAATATTATCTCCTGCAATTGGTGGAATAATTGTG
GGTTATGAGATTGGCAGGCAGCTTGGAATAAGGACAATGTTTTGCGAGCGCGTTAATGGCAAGTTTGAATTACGCCGTGG
ATTTGAAATTAAAAAAGGTGAAAAAATATTACTTATCGAGGATGTGATAACCACAGGCAAATCTTCACTTGAAGCAGTGG
AATGTGCAGAAGGGGAAGGAGGTAAAGTTGTTGCTGAAGCTTCTTTGATAAAGAGAAATAGTGAAACGAAACTGCCTTTT
CCTGTCATATCTTTGATTGAACTGAACATCAAAAACTACAGCGAGGAAGAACTTCCAAGTGAATTAAAACAACTACCCAT
AACAAAACCTGGGAGTAGAGAATATTTAACAAAGTAA

Upstream 100 bases:

>100_bases
TTATACAATCATCGATATGTTTGAAACTGCAATAAATGTGTGGTCTGATATTTGCATAACTCAGATAATTAATAAAGAAT
GTAATAGTGAAACTTAAAAA

Downstream 100 bases:

>100_bases
TCCTATAATATCTTGGAAGCACAGCACAGCTTTAGTTATTTACTCTAAGTGTATTTTATGTATACTTATTACTAAATTAA
TTTTGAAATGATAGAAAATG

Product: orotate phosphoribosyltransferase

Products: NA

Alternate protein names: OPRT; OPRTase [H]

Number of amino acids: Translated: 198; Mature: 198

Protein sequence:

>198_residues
MILDENNLIIKEFKEAEAILHGHFILSSGLHSNTYIQCAKIFENPSRAMKVCALLADKIKKELNSSIDLILSPAIGGIIV
GYEIGRQLGIRTMFCERVNGKFELRRGFEIKKGEKILLIEDVITTGKSSLEAVECAEGEGGKVVAEASLIKRNSETKLPF
PVISLIELNIKNYSEEELPSELKQLPITKPGSREYLTK

Sequences:

>Translated_198_residues
MILDENNLIIKEFKEAEAILHGHFILSSGLHSNTYIQCAKIFENPSRAMKVCALLADKIKKELNSSIDLILSPAIGGIIV
GYEIGRQLGIRTMFCERVNGKFELRRGFEIKKGEKILLIEDVITTGKSSLEAVECAEGEGGKVVAEASLIKRNSETKLPF
PVISLIELNIKNYSEEELPSELKQLPITKPGSREYLTK
>Mature_198_residues
MILDENNLIIKEFKEAEAILHGHFILSSGLHSNTYIQCAKIFENPSRAMKVCALLADKIKKELNSSIDLILSPAIGGIIV
GYEIGRQLGIRTMFCERVNGKFELRRGFEIKKGEKILLIEDVITTGKSSLEAVECAEGEGGKVVAEASLIKRNSETKLPF
PVISLIELNIKNYSEEELPSELKQLPITKPGSREYLTK

Specific function: Catalyzes the transfer of a ribosyl phosphate group from 5-phosphoribose 1-diphosphate to orotate, leading to the formation of orotidine monophosphate (OMP) [H]

COG id: COG0461

COG function: function code F; Orotate phosphoribosyltransferase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the purine/pyrimidine phosphoribosyltransferase family. PyrE subfamily [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR023031
- InterPro:   IPR006273
- InterPro:   IPR000836 [H]

Pfam domain/function: PF00156 Pribosyltran [H]

EC number: =2.4.2.10 [H]

Molecular weight: Translated: 22017; Mature: 22017

Theoretical pI: Translated: 6.78; Mature: 6.78

Prosite motif: PS00103 PUR_PYR_PR_TRANSFER

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MILDENNLIIKEFKEAEAILHGHFILSSGLHSNTYIQCAKIFENPSRAMKVCALLADKIK
CEECCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHH
KELNSSIDLILSPAIGGIIVGYEIGRQLGIRTMFCERVNGKFELRRGFEIKKGEKILLIE
HHHCCCEEEEECCCCCCEEEHHHHHHHHCHHHHHHHHCCCEEEEECCCEECCCCEEEEEH
DVITTGKSSLEAVECAEGEGGKVVAEASLIKRNSETKLPFPVISLIELNIKNYSEEELPS
HHHHCCCCHHHHHHHCCCCCCEEEEEHHHHHCCCCCCCCCHHHHHEEECCCCCCHHHHHH
ELKQLPITKPGSREYLTK
HHHHCCCCCCCCCCCCCC
>Mature Secondary Structure
MILDENNLIIKEFKEAEAILHGHFILSSGLHSNTYIQCAKIFENPSRAMKVCALLADKIK
CEECCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHH
KELNSSIDLILSPAIGGIIVGYEIGRQLGIRTMFCERVNGKFELRRGFEIKKGEKILLIE
HHHCCCEEEEECCCCCCEEEHHHHHHHHCHHHHHHHHCCCEEEEECCCEECCCCEEEEEH
DVITTGKSSLEAVECAEGEGGKVVAEASLIKRNSETKLPFPVISLIELNIKNYSEEELPS
HHHHCCCCHHHHHHHCCCCCCEEEEEHHHHHCCCCCCCCCHHHHHEEECCCCCCHHHHHH
ELKQLPITKPGSREYLTK
HHHHCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA