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The map label for this gene is gyrB [H]

Identifier: 225629988

GI number: 225629988

Start: 140397

End: 142826

Strand: Reverse

Name: gyrB [H]

Synonym: WRi_001420

Alternate gene names: 225629988

Gene position: 142826-140397 (Counterclockwise)

Preceding gene: 225629989

Following gene: 225629973

Centisome position: 9.88

GC content: 34.49

Gene sequence:

>2430_bases
ATGAATGTAATATTAATTAACATTCATAAACGAAGCAATACCATGGAAAATAGTTATAATGCCGATGCAATAAAAATCTT
AAGGGGTCTTGATGCTGTAAGGAAACGTCCAGGTATGTACATTGGTGACACTGACGATGGATCTGGCTTGCACCACATGG
TATATGAGGTTATTGACAACGCAATAGATGAGTCTTTAGCTGGATATTGTGATAAAATTGAAGTTAGCATAAATAAAGAT
GGTTCAGTATCTGTAACTGATAATGGTCGTGGCATTCCAACTGACATTCATGAAGAAGAAGGAATATCAGCAGCAGAGGT
AATAATGACTCAACTCCATGCTGGTGGTAAATTTGACAGTAATACCTATAAAGTTTCTGGGGGATTACATGGTGTTGGAA
TCTCAGTTGTGAATGCATTATCAAGCTGGTTGGAATTAACTATTTGGCGCAATAAGAAAGAACACTTTATGCGTTTTGAA
GATGGTGAGTCTATTGAACCTTTAAAGGTAGTCAACGAAAATACAAACAAAAGAGGAACTAAAGTCACGTTCATGCCATC
AACAGAGACTTTTAATGGCATTAATTTCAGTTACTCAACGCTTGAAAGTCGTATAAGAGAATTAGCATTTTTAAATTCAA
ATATCGACATTACTTTACGTGATCTGCGCAATGAACCATATACAGAATCTCATTTCAATCAGAGCAATCAATCTAAAGAC
AATTTTGGTACAGCAAATTTTGTGCAGTATTTAGATAAGAATAAGACACATGTAACTAAAATTGCCAGTATGAAAGGAGA
TGCAGAAGATCTTGGCATCAGCTTGGAAATATCGATGGAATGGAATGATTCCTACTATGAGAATATGTTGTGTTTCACTA
ATAATATACGGCAACGAGATGGTGGTACGCATTTGGCAGGATTTAGATCTGCACTAACTAGATGTATCAATACCTATGCA
ACTAATGAAGGGTTTTTAAAGAAAGCAAAAGTAAATTTAACTGGAGAGGACGTTAGAGAAGGTTTAACTTGTGTTTTATC
TCTTAAGATGCCTGATCCTAAGTTTTCTTCACAAACAAAAGATAAACTAGTTAGTTCTGAAGCACGTGCAGTTGTAGAAA
GTATAGTTTCTGATAAATTGAGCACAATCCTTGAAACTGATCCCAAATTGGCAGCAAGCATAGTAGAAAGAGCAATCAGG
TCAGCAAAAGGAAGAGAAGCTGCAAGAAAAGCGCGCGAGCTAGTTAAAAGCAAAAACAATATTGATATTGCAACTCTGCC
AGGAAAGCTTGCTGATTGTCAGGAAAAAGCTCCTGAGTTATCGGAATTATTTATAGTAGAGGGTAATTCAGCAGGTGGTA
CTGCAAAGCAGGGGCGTAATCGTAAAACACAAGCAGTGCTTGCCTTAAGAGGAAAAATTCTAAACGTAGAACGTGTAAGT
TTAGATCGTATTTTTTCATCTGCAGAAATAGGCTCTTTAATTACTGCAATTGGTGCTGGAATAGGGAGTGAACACTTCGA
TATTGAAAAAGCTAGATATCATAAAATTATCATCATGACAGATGCAGATGTTGATGGTTCACATATAAGAACTTTGATTC
TGACTTTCTTTTTTAGACACATGCGTGAAGTTATTGAAAAAGGCTACTTATACATAGCACAGCCACCCCTCTATAAAGTT
ACAAAGAATGCTAAAGACACTTATATTAAAGATGACGAAACTTTTGAAGAGTATATAATAAACTCAGCAGTTAAAAGATT
AACACTAAGTAGTACAGCTACAGAATTTAACGATTTGCGCCTTATTTTGAATAAATGCGTTAGCATTTCTAATATTAGCA
AAAATTATGATAGAGAGATACCACAAAATCTCTTAGAGTCATTGCTAATTTTAAGCAAAAAGAATGCTCTATCATCGGCT
GATGATATATTAAAGTATTTGAAGTTAATGTACAGCGAATATACTTGGGAAGTGGAAATAAAAGATGAAGAAAAAGAAAT
TCACATCTCTAAATTGTTTCAAGGATTGGCAGACAAATATATATTTGCACTTAGTATGCTTGAAGGCAAAGATATGCAAA
ACATATTAAGTTTGCTTGATGGTATAAGTAATTTATTTAATGGTGATTCATTTTTAAAATCACAAGAAACTGAAATAAGA
ATCACTTCTCCAAGTACGCTTGCAAAAATAATAATGGACTATGGTAAAAAGGGTTTAACTCTGCAGAGATTTAAAGGTCT
TGGTGAAATGAATGCTGATCAGCTATGGGATACTACGTTAAATCCTGAGGCTAGAACTTTACTAAAGGTTGAAATAAAAG
ATTGTGAAGAAGCTGATTCCATATTTTCAATACTAATGGGTGATATAGTTGAACCACGCCGTGATTTTATTAATAGCAAT
GCACTCAACGTGCATGATGTTGATATTTGA

Upstream 100 bases:

>100_bases
ACATTGGAATGACACTATTTGCTGTCTTCAAAAATAAACATTCGTAAAGTTGTGCTTTTAATATGGGTTATCAGTTTAAA
AATGTTGCTAATCAGCATAT

Downstream 100 bases:

>100_bases
ACTTTTAGCAAAATGTACGAACGTTACGGGTTTAAGCAAAGACTGTGTCATTCAAGTAGCTGACACTGGTTCCTTTATAA
CGGTGTCATTCCAGTGCTTG

Product: DNA gyrase, B subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 809; Mature: 809

Protein sequence:

>809_residues
MNVILINIHKRSNTMENSYNADAIKILRGLDAVRKRPGMYIGDTDDGSGLHHMVYEVIDNAIDESLAGYCDKIEVSINKD
GSVSVTDNGRGIPTDIHEEEGISAAEVIMTQLHAGGKFDSNTYKVSGGLHGVGISVVNALSSWLELTIWRNKKEHFMRFE
DGESIEPLKVVNENTNKRGTKVTFMPSTETFNGINFSYSTLESRIRELAFLNSNIDITLRDLRNEPYTESHFNQSNQSKD
NFGTANFVQYLDKNKTHVTKIASMKGDAEDLGISLEISMEWNDSYYENMLCFTNNIRQRDGGTHLAGFRSALTRCINTYA
TNEGFLKKAKVNLTGEDVREGLTCVLSLKMPDPKFSSQTKDKLVSSEARAVVESIVSDKLSTILETDPKLAASIVERAIR
SAKGREAARKARELVKSKNNIDIATLPGKLADCQEKAPELSELFIVEGNSAGGTAKQGRNRKTQAVLALRGKILNVERVS
LDRIFSSAEIGSLITAIGAGIGSEHFDIEKARYHKIIIMTDADVDGSHIRTLILTFFFRHMREVIEKGYLYIAQPPLYKV
TKNAKDTYIKDDETFEEYIINSAVKRLTLSSTATEFNDLRLILNKCVSISNISKNYDREIPQNLLESLLILSKKNALSSA
DDILKYLKLMYSEYTWEVEIKDEEKEIHISKLFQGLADKYIFALSMLEGKDMQNILSLLDGISNLFNGDSFLKSQETEIR
ITSPSTLAKIIMDYGKKGLTLQRFKGLGEMNADQLWDTTLNPEARTLLKVEIKDCEEADSIFSILMGDIVEPRRDFINSN
ALNVHDVDI

Sequences:

>Translated_809_residues
MNVILINIHKRSNTMENSYNADAIKILRGLDAVRKRPGMYIGDTDDGSGLHHMVYEVIDNAIDESLAGYCDKIEVSINKD
GSVSVTDNGRGIPTDIHEEEGISAAEVIMTQLHAGGKFDSNTYKVSGGLHGVGISVVNALSSWLELTIWRNKKEHFMRFE
DGESIEPLKVVNENTNKRGTKVTFMPSTETFNGINFSYSTLESRIRELAFLNSNIDITLRDLRNEPYTESHFNQSNQSKD
NFGTANFVQYLDKNKTHVTKIASMKGDAEDLGISLEISMEWNDSYYENMLCFTNNIRQRDGGTHLAGFRSALTRCINTYA
TNEGFLKKAKVNLTGEDVREGLTCVLSLKMPDPKFSSQTKDKLVSSEARAVVESIVSDKLSTILETDPKLAASIVERAIR
SAKGREAARKARELVKSKNNIDIATLPGKLADCQEKAPELSELFIVEGNSAGGTAKQGRNRKTQAVLALRGKILNVERVS
LDRIFSSAEIGSLITAIGAGIGSEHFDIEKARYHKIIIMTDADVDGSHIRTLILTFFFRHMREVIEKGYLYIAQPPLYKV
TKNAKDTYIKDDETFEEYIINSAVKRLTLSSTATEFNDLRLILNKCVSISNISKNYDREIPQNLLESLLILSKKNALSSA
DDILKYLKLMYSEYTWEVEIKDEEKEIHISKLFQGLADKYIFALSMLEGKDMQNILSLLDGISNLFNGDSFLKSQETEIR
ITSPSTLAKIIMDYGKKGLTLQRFKGLGEMNADQLWDTTLNPEARTLLKVEIKDCEEADSIFSILMGDIVEPRRDFINSN
ALNVHDVDI
>Mature_809_residues
MNVILINIHKRSNTMENSYNADAIKILRGLDAVRKRPGMYIGDTDDGSGLHHMVYEVIDNAIDESLAGYCDKIEVSINKD
GSVSVTDNGRGIPTDIHEEEGISAAEVIMTQLHAGGKFDSNTYKVSGGLHGVGISVVNALSSWLELTIWRNKKEHFMRFE
DGESIEPLKVVNENTNKRGTKVTFMPSTETFNGINFSYSTLESRIRELAFLNSNIDITLRDLRNEPYTESHFNQSNQSKD
NFGTANFVQYLDKNKTHVTKIASMKGDAEDLGISLEISMEWNDSYYENMLCFTNNIRQRDGGTHLAGFRSALTRCINTYA
TNEGFLKKAKVNLTGEDVREGLTCVLSLKMPDPKFSSQTKDKLVSSEARAVVESIVSDKLSTILETDPKLAASIVERAIR
SAKGREAARKARELVKSKNNIDIATLPGKLADCQEKAPELSELFIVEGNSAGGTAKQGRNRKTQAVLALRGKILNVERVS
LDRIFSSAEIGSLITAIGAGIGSEHFDIEKARYHKIIIMTDADVDGSHIRTLILTFFFRHMREVIEKGYLYIAQPPLYKV
TKNAKDTYIKDDETFEEYIINSAVKRLTLSSTATEFNDLRLILNKCVSISNISKNYDREIPQNLLESLLILSKKNALSSA
DDILKYLKLMYSEYTWEVEIKDEEKEIHISKLFQGLADKYIFALSMLEGKDMQNILSLLDGISNLFNGDSFLKSQETEIR
ITSPSTLAKIIMDYGKKGLTLQRFKGLGEMNADQLWDTTLNPEARTLLKVEIKDCEEADSIFSILMGDIVEPRRDFINSN
ALNVHDVDI

Specific function: DNA gyrase negatively supercoils closed circular double- stranded DNA in an ATP-dependent manner and also catalyzes the interconversion of other topological isomers of double-stranded DNA rings, including catenanes and knotted rings [H]

COG id: COG0187

COG function: function code L; Type IIA topoisomerase (DNA gyrase/topo II, topoisomerase IV), B subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Homo sapiens, GI19913406, Length=577, Percent_Identity=24.263431542461, Blast_Score=117, Evalue=5e-26,
Organism=Homo sapiens, GI19913408, Length=585, Percent_Identity=23.5897435897436, Blast_Score=106, Evalue=7e-23,
Organism=Escherichia coli, GI48994957, Length=815, Percent_Identity=50.3067484662577, Blast_Score=776, Evalue=0.0,
Organism=Escherichia coli, GI1789408, Length=549, Percent_Identity=40.0728597449909, Blast_Score=379, Evalue=1e-106,
Organism=Caenorhabditis elegans, GI17535065, Length=558, Percent_Identity=23.2974910394265, Blast_Score=114, Evalue=2e-25,
Organism=Caenorhabditis elegans, GI212645845, Length=518, Percent_Identity=24.1312741312741, Blast_Score=103, Evalue=3e-22,
Organism=Saccharomyces cerevisiae, GI6324241, Length=601, Percent_Identity=25.9567387687188, Blast_Score=119, Evalue=2e-27,
Organism=Drosophila melanogaster, GI17136538, Length=567, Percent_Identity=24.1622574955908, Blast_Score=119, Evalue=1e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR011557
- InterPro:   IPR020568
- InterPro:   IPR014721
- InterPro:   IPR001241
- InterPro:   IPR013759
- InterPro:   IPR002288
- InterPro:   IPR013506
- InterPro:   IPR013760
- InterPro:   IPR018522
- InterPro:   IPR006171 [H]

Pfam domain/function: PF00204 DNA_gyraseB; PF00986 DNA_gyraseB_C; PF02518 HATPase_c; PF01751 Toprim [H]

EC number: =5.99.1.3 [H]

Molecular weight: Translated: 90625; Mature: 90625

Theoretical pI: Translated: 5.72; Mature: 5.72

Prosite motif: PS00177 TOPOISOMERASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNVILINIHKRSNTMENSYNADAIKILRGLDAVRKRPGMYIGDTDDGSGLHHMVYEVIDN
CCEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHCCCCEECCCCCCCCHHHHHHHHHHH
AIDESLAGYCDKIEVSINKDGSVSVTDNGRGIPTDIHEEEGISAAEVIMTQLHAGGKFDS
HHHHHHHCCCCEEEEEECCCCCEEECCCCCCCCCCCCHHCCCCHHHHHHHHHHCCCCCCC
NTYKVSGGLHGVGISVVNALSSWLELTIWRNKKEHFMRFEDGESIEPLKVVNENTNKRGT
CCEEECCCCCHHHHHHHHHHHHHHHEEHCCCCHHHCCCCCCCCCCCCHHHCCCCCCCCCC
KVTFMPSTETFNGINFSYSTLESRIRELAFLNSNIDITLRDLRNEPYTESHFNQSNQSKD
EEEEECCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCHHHCCCCCCCCC
NFGTANFVQYLDKNKTHVTKIASMKGDAEDLGISLEISMEWNDSYYENMLCFTNNIRQRD
CCCHHHHHHHHHCCCHHHHHHHHCCCCHHHCCEEEEEEEEECCHHHHHHHHHHCCHHHCC
GGTHLAGFRSALTRCINTYATNEGFLKKAKVNLTGEDVREGLTCVLSLKMPDPKFSSQTK
CCCHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCHHHHHCCCEEEEEEECCCCCCCCHHH
DKLVSSEARAVVESIVSDKLSTILETDPKLAASIVERAIRSAKGREAARKARELVKSKNN
HHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCC
IDIATLPGKLADCQEKAPELSELFIVEGNSAGGTAKQGRNRKTQAVLALRGKILNVERVS
CEEEECCCHHHHHHHHCCCCEEEEEEECCCCCCCCCCCCCCHHHHHHHHHCCEECCHHHH
LDRIFSSAEIGSLITAIGAGIGSEHFDIEKARYHKIIIMTDADVDGSHIRTLILTFFFRH
HHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHH
MREVIEKGYLYIAQPPLYKVTKNAKDTYIKDDETFEEYIINSAVKRLTLSSTATEFNDLR
HHHHHHCCCEEEECCCCHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LILNKCVSISNISKNYDREIPQNLLESLLILSKKNALSSADDILKYLKLMYSEYTWEVEI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCEEEEEE
KDEEKEIHISKLFQGLADKYIFALSMLEGKDMQNILSLLDGISNLFNGDSFLKSQETEIR
CCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCHHHHCCCCCEEE
ITSPSTLAKIIMDYGKKGLTLQRFKGLGEMNADQLWDTTLNPEARTLLKVEIKDCEEADS
EECHHHHHHHHHHHCCCCCCHHHHCCCCCCCHHHHCCCCCCCCCEEEEEEEECCCHHHHH
IFSILMGDIVEPRRDFINSNALNVHDVDI
HHHHHHHHHHHHHHHHCCCCCCCEEECCC
>Mature Secondary Structure
MNVILINIHKRSNTMENSYNADAIKILRGLDAVRKRPGMYIGDTDDGSGLHHMVYEVIDN
CCEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHCCCCEECCCCCCCCHHHHHHHHHHH
AIDESLAGYCDKIEVSINKDGSVSVTDNGRGIPTDIHEEEGISAAEVIMTQLHAGGKFDS
HHHHHHHCCCCEEEEEECCCCCEEECCCCCCCCCCCCHHCCCCHHHHHHHHHHCCCCCCC
NTYKVSGGLHGVGISVVNALSSWLELTIWRNKKEHFMRFEDGESIEPLKVVNENTNKRGT
CCEEECCCCCHHHHHHHHHHHHHHHEEHCCCCHHHCCCCCCCCCCCCHHHCCCCCCCCCC
KVTFMPSTETFNGINFSYSTLESRIRELAFLNSNIDITLRDLRNEPYTESHFNQSNQSKD
EEEEECCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCHHHCCCCCCCCC
NFGTANFVQYLDKNKTHVTKIASMKGDAEDLGISLEISMEWNDSYYENMLCFTNNIRQRD
CCCHHHHHHHHHCCCHHHHHHHHCCCCHHHCCEEEEEEEEECCHHHHHHHHHHCCHHHCC
GGTHLAGFRSALTRCINTYATNEGFLKKAKVNLTGEDVREGLTCVLSLKMPDPKFSSQTK
CCCHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCHHHHHCCCEEEEEEECCCCCCCCHHH
DKLVSSEARAVVESIVSDKLSTILETDPKLAASIVERAIRSAKGREAARKARELVKSKNN
HHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCC
IDIATLPGKLADCQEKAPELSELFIVEGNSAGGTAKQGRNRKTQAVLALRGKILNVERVS
CEEEECCCHHHHHHHHCCCCEEEEEEECCCCCCCCCCCCCCHHHHHHHHHCCEECCHHHH
LDRIFSSAEIGSLITAIGAGIGSEHFDIEKARYHKIIIMTDADVDGSHIRTLILTFFFRH
HHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHH
MREVIEKGYLYIAQPPLYKVTKNAKDTYIKDDETFEEYIINSAVKRLTLSSTATEFNDLR
HHHHHHCCCEEEECCCCHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LILNKCVSISNISKNYDREIPQNLLESLLILSKKNALSSADDILKYLKLMYSEYTWEVEI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCEEEEEE
KDEEKEIHISKLFQGLADKYIFALSMLEGKDMQNILSLLDGISNLFNGDSFLKSQETEIR
CCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCHHHHCCCCCEEE
ITSPSTLAKIIMDYGKKGLTLQRFKGLGEMNADQLWDTTLNPEARTLLKVEIKDCEEADS
EECHHHHHHHHHHHCCCCCCHHHHCCCCCCCHHHHCCCCCCCCCEEEEEEEECCCHHHHH
IFSILMGDIVEPRRDFINSNALNVHDVDI
HHHHHHHHHHHHHHHHCCCCCCCEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA