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The map label for this gene is pheS [H]

Identifier: 225629973

GI number: 225629973

Start: 121882

End: 122925

Strand: Reverse

Name: pheS [H]

Synonym: WRi_001200

Alternate gene names: 225629973

Gene position: 122925-121882 (Counterclockwise)

Preceding gene: 225629988

Following gene: 225629972

Centisome position: 8.5

GC content: 35.06

Gene sequence:

>1044_bases
ATGAATAAAGAGTTATTAGATGACATACTTTCACTTGAAGATAAAGCAGTTTCCGAAATTGAGAATGCTTCTTCTTTACA
AGATTTAGAAAAAGTTAGGCTATCATATTTGGGAAAAAAGGGTGTAATTAAAGCTTATTTTGACAACTTAAAAGAGATAG
AAGACGCAGGAAAAAAGCGCAATTTAGGCGAAGTTATCAACGTTTTACGTAATAAGCTAGATCAGCTTATAATGAATAAG
GAGAATATACTAAAAGCCGAAGAAGTTAACTTTAAATTGCAGAACGAGGCAGTTGATATCACGTTGCCTGCGAGGCCAGA
AAAAATTGGCAAGGTCCATCCACTCAGTAAAGTTATAAATGAAGTAAAGCTTATTTTTGCACATATGGGTTTTAAAGCAG
TTGATGGCCCTGATATTGAAGATGAATTTCATGTATTTGATGCACTGAATACTCCAAGTCATCATCCTGCGCGAGAGGAG
CAAGATACCTTCTACTTAAAGAATAAAAGAAACGATAAAAGAATGGTACTGCGCACTCACACTTCATCTGTACAGATTAG
AACCATGGAAAAAACAAAAAAATTCCCAATTAAAATAGTAGCCCCTGGTAGGGTATACAGAAATGACTTTGATGCAACTC
ATACTCCTATGTTTCACCAGATAGAAGGGTTATATGTCGATGAAAACGTCAATATGGGCCAGTTAAAATTTACTATTCAT
CACTTCCTTAATAAGTTTTTTGGAGATAAAGGGCTGAAGATACGTTTTCGTAATAGTTTTTTTCCTTTTACTGAACCCTC
TGCGGAAGTGGACATAAGTTATAAAGGTAGTAAATGGATAGAAGTGCTTGGATGTGGTATGGTGCATCCAAATGTCTTTC
AAAATGTTGGAATAGACCATACTAAATACAACGGTTTTGCGTTTGGCATTGGTATAGAAAGGCTTGCGATGCTAAAATAT
CAAATCAGCGACCTAAGAAGCTTTTACGATAACAAAATCAGCTGGCTTGATCATTACGGTTTTCATTTTTCATCTTTAAG
ATAA

Upstream 100 bases:

>100_bases
AATTAGTCAATAAATTAATAATCAGAATAGGGCAATTTTTCGGATTGTTGATGATATTTAAAATTTCACTATAATGAAAA
TTTAAGCCAGCGTTTTTAAG

Downstream 100 bases:

>100_bases
GTGATAAGTAAAACTCATACGTTCGTTATACTTGCTGCAGGGCATGGCAGGCGGATGAATTCAGATTTGCCTAAGGTCCT
ACACAAAATAGGCAATTTTT

Product: phenylalanyl-tRNA synthetase subunit alpha

Products: NA

Alternate protein names: Phenylalanine--tRNA ligase alpha chain; PheRS [H]

Number of amino acids: Translated: 347; Mature: 347

Protein sequence:

>347_residues
MNKELLDDILSLEDKAVSEIENASSLQDLEKVRLSYLGKKGVIKAYFDNLKEIEDAGKKRNLGEVINVLRNKLDQLIMNK
ENILKAEEVNFKLQNEAVDITLPARPEKIGKVHPLSKVINEVKLIFAHMGFKAVDGPDIEDEFHVFDALNTPSHHPAREE
QDTFYLKNKRNDKRMVLRTHTSSVQIRTMEKTKKFPIKIVAPGRVYRNDFDATHTPMFHQIEGLYVDENVNMGQLKFTIH
HFLNKFFGDKGLKIRFRNSFFPFTEPSAEVDISYKGSKWIEVLGCGMVHPNVFQNVGIDHTKYNGFAFGIGIERLAMLKY
QISDLRSFYDNKISWLDHYGFHFSSLR

Sequences:

>Translated_347_residues
MNKELLDDILSLEDKAVSEIENASSLQDLEKVRLSYLGKKGVIKAYFDNLKEIEDAGKKRNLGEVINVLRNKLDQLIMNK
ENILKAEEVNFKLQNEAVDITLPARPEKIGKVHPLSKVINEVKLIFAHMGFKAVDGPDIEDEFHVFDALNTPSHHPAREE
QDTFYLKNKRNDKRMVLRTHTSSVQIRTMEKTKKFPIKIVAPGRVYRNDFDATHTPMFHQIEGLYVDENVNMGQLKFTIH
HFLNKFFGDKGLKIRFRNSFFPFTEPSAEVDISYKGSKWIEVLGCGMVHPNVFQNVGIDHTKYNGFAFGIGIERLAMLKY
QISDLRSFYDNKISWLDHYGFHFSSLR
>Mature_347_residues
MNKELLDDILSLEDKAVSEIENASSLQDLEKVRLSYLGKKGVIKAYFDNLKEIEDAGKKRNLGEVINVLRNKLDQLIMNK
ENILKAEEVNFKLQNEAVDITLPARPEKIGKVHPLSKVINEVKLIFAHMGFKAVDGPDIEDEFHVFDALNTPSHHPAREE
QDTFYLKNKRNDKRMVLRTHTSSVQIRTMEKTKKFPIKIVAPGRVYRNDFDATHTPMFHQIEGLYVDENVNMGQLKFTIH
HFLNKFFGDKGLKIRFRNSFFPFTEPSAEVDISYKGSKWIEVLGCGMVHPNVFQNVGIDHTKYNGFAFGIGIERLAMLKY
QISDLRSFYDNKISWLDHYGFHFSSLR

Specific function: Unknown

COG id: COG0016

COG function: function code J; Phenylalanyl-tRNA synthetase alpha subunit

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. Phe-tRNA synthetase alpha chain type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI4758340, Length=266, Percent_Identity=32.3308270676692, Blast_Score=131, Evalue=7e-31,
Organism=Homo sapiens, GI5729820, Length=220, Percent_Identity=32.2727272727273, Blast_Score=108, Evalue=6e-24,
Organism=Escherichia coli, GI1788007, Length=334, Percent_Identity=48.2035928143713, Blast_Score=337, Evalue=8e-94,
Organism=Caenorhabditis elegans, GI17508957, Length=265, Percent_Identity=30.5660377358491, Blast_Score=126, Evalue=2e-29,
Organism=Caenorhabditis elegans, GI32563657, Length=265, Percent_Identity=30.5660377358491, Blast_Score=125, Evalue=2e-29,
Organism=Caenorhabditis elegans, GI32566635, Length=246, Percent_Identity=31.7073170731707, Blast_Score=114, Evalue=7e-26,
Organism=Saccharomyces cerevisiae, GI6321087, Length=266, Percent_Identity=31.9548872180451, Blast_Score=137, Evalue=2e-33,
Organism=Saccharomyces cerevisiae, GI6325304, Length=262, Percent_Identity=26.3358778625954, Blast_Score=100, Evalue=3e-22,
Organism=Drosophila melanogaster, GI18858079, Length=363, Percent_Identity=27.2727272727273, Blast_Score=122, Evalue=5e-28,
Organism=Drosophila melanogaster, GI17137424, Length=244, Percent_Identity=28.6885245901639, Blast_Score=101, Evalue=6e-22,

Paralogues:

None

Copy number: 2,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006195
- InterPro:   IPR004529
- InterPro:   IPR004188
- InterPro:   IPR022911
- InterPro:   IPR002319
- InterPro:   IPR010978 [H]

Pfam domain/function: PF02912 Phe_tRNA-synt_N; PF01409 tRNA-synt_2d [H]

EC number: =6.1.1.20 [H]

Molecular weight: Translated: 40110; Mature: 40110

Theoretical pI: Translated: 8.79; Mature: 8.79

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKELLDDILSLEDKAVSEIENASSLQDLEKVRLSYLGKKGVIKAYFDNLKEIEDAGKKR
CCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCC
NLGEVINVLRNKLDQLIMNKENILKAEEVNFKLQNEAVDITLPARPEKIGKVHPLSKVIN
CHHHHHHHHHHHHHHHHHCHHHCCCHHHCCEEEECCEEEEEECCCCHHHCCCCHHHHHHH
EVKLIFAHMGFKAVDGPDIEDEFHVFDALNTPSHHPAREEQDTFYLKNKRNDKRMVLRTH
HHHHHHHHCCCEECCCCCCCCCHHEEECCCCCCCCCCCCCCCEEEEECCCCCCEEEEEEC
TSSVQIRTMEKTKKFPIKIVAPGRVYRNDFDATHTPMFHQIEGLYVDENVNMGQLKFTIH
CCCEEEEEHHHCCCCCEEEEECCHHCCCCCCCCCCCHHHHCCCEEEECCCCCCHHHHHHH
HFLNKFFGDKGLKIRFRNSFFPFTEPSAEVDISYKGSKWIEVLGCGMVHPNVFQNVGIDH
HHHHHHHCCCCCEEEECCCCCCCCCCCCCEEEEECCCCEEHHEECCCCCCHHHHHCCCCC
TKYNGFAFGIGIERLAMLKYQISDLRSFYDNKISWLDHYGFHFSSLR
EECCCEEEEHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCHHCCCC
>Mature Secondary Structure
MNKELLDDILSLEDKAVSEIENASSLQDLEKVRLSYLGKKGVIKAYFDNLKEIEDAGKKR
CCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCC
NLGEVINVLRNKLDQLIMNKENILKAEEVNFKLQNEAVDITLPARPEKIGKVHPLSKVIN
CHHHHHHHHHHHHHHHHHCHHHCCCHHHCCEEEECCEEEEEECCCCHHHCCCCHHHHHHH
EVKLIFAHMGFKAVDGPDIEDEFHVFDALNTPSHHPAREEQDTFYLKNKRNDKRMVLRTH
HHHHHHHHCCCEECCCCCCCCCHHEEECCCCCCCCCCCCCCCEEEEECCCCCCEEEEEEC
TSSVQIRTMEKTKKFPIKIVAPGRVYRNDFDATHTPMFHQIEGLYVDENVNMGQLKFTIH
CCCEEEEEHHHCCCCCEEEEECCHHCCCCCCCCCCCHHHHCCCEEEECCCCCCHHHHHHH
HFLNKFFGDKGLKIRFRNSFFPFTEPSAEVDISYKGSKWIEVLGCGMVHPNVFQNVGIDH
HHHHHHHCCCCCEEEECCCCCCCCCCCCCEEEEECCCCEEHHEECCCCCCHHHHHCCCCC
TKYNGFAFGIGIERLAMLKYQISDLRSFYDNKISWLDHYGFHFSSLR
EECCCEEEEHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA