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The map label for this gene is ydaB [H]

Identifier: 222527029

GI number: 222527029

Start: 4739824

End: 4741518

Strand: Reverse

Name: ydaB [H]

Synonym: Chy400_3808

Alternate gene names: 222527029

Gene position: 4741518-4739824 (Counterclockwise)

Preceding gene: 222527030

Following gene: 222527028

Centisome position: 89.99

GC content: 59.47

Gene sequence:

>1695_bases
ATGCGCAACGAAGAACAGACCGCTGCGCGCGTCACCTTCACAGTGGTGGCCGGCGCGAACGTTGCCCGCCACCTGCCCTT
GATGGCCCGGGAGCGTCCGGATCAGACCGCTGTTGTCAGTGGGGTTGGACGGGATCGGGCAGGGAAGGTCATCTACCGTA
GACAGAGCTTTGCCGGTTTACACGCGGCCAGTGATCGGCTGGCGTGGGGGCTGACGGCTTATGGGTTACGGAAGGGAATG
CGGGTTTTGCTGATGGTTCCGGCGGGTGTGCCGCTGATCAGCCTTACCTTTGCGTTGATGAAGGCCGGTTGTGTGCCGAT
TCTGATTGACCCGGCAATGGGGCGGCGGAATCTGGCTCAGTGCATTGCCGAAGTCGAACCAGAGGCATTCATCGGGGTAC
CGCGGGCGCATCTGTTACGGCTGATCTTTCCCCGCTCCTGTGCGACCATTAGATATGCCGTCTCGGTAGGGCCAGCCTTG
CCCGGTGCTGCTGCCTTACGCGAGCTTGACATACCGATGAAGACAGCGTTTCCGCTGGCGGATATGCGATCTGATGATCC
GGCAGCAATTGTCTTTACCAGTGGTAGCACCGGTACCCCGAAAGGTGTGCTCTATACGCACGGGATGTTTGAGGCGCAGA
TTCATACGCTGCGCGATCTGTTTGGGATTGCCGCGGGTGAGGTCGAGATGCCGGCCTTCCCGCTCTTCGCCCTGTTCAAT
GTTGCCCTGGGGGTGACATCGGCAATTCCGCCCATCGATCCGACCCGACCGGCGCAGTGCGATCCGGCAGCCGTGGTTGA
GTTTATCCGTGATCTCGGCGTAACATCGACCTTCGGTTCACCGGCAATCTGGGAAAAGGTGACAGCGTATTGCCTGACCC
ACGGTATTCAACTACCGTCGCTCCGGCGGGTGTTGATGGCGGGAGCACCGGTGCCAATCCATCTGCACGAGCGTTTGCAT
CGCATTCTATCACCTACGGCTGATAGCTTTACCCCTTACGGAGCCACCGAAGCGTTGCCGGTAACTTCAATCAGCGGTCG
TGAGGTACTGGCAGCGCATACCGAATACCCCTCACCACTGGCCGGGACATGTATTGGCTATCCGGTTCCCGGCGTTGAGG
TCGCGATTATTCCGATCACCGATGAGCCAATTCACGCCTGGCAGGATACGCACCGCTTGCCGCCCGGCGTCATTGGCGAG
ATTTGTGTGAGTGGTGCCACCGTCACCCATACCTACGTCGGGCGGCCCCAGGCGACCGCGTTAGCCAAAATCAGTGATGG
CAACCGCATCTGGCACCGCATGGGTGATCTCGGTTATTTCGACGAGCGAGGGCGGCTCTGGTTTTACGGGCGTAAAAGTC
AGCGCGTGATTACGGCGCATGGAACGCTCTTCACCGAGCCGGTTGAGCGCCTCTTCAACCAGCATCCGGCAGTTGCCCGT
TCGGCGCTGGTGGGGATCGGGAAGCCCGGCACACAATTGCCGGTCGTCGTTGTCGAACGGCGGCGTGATCGGGCCATTTC
AACCTCACAACTGATCCGTGAGCTGCGCCAACTGGCGACCACAACCGAAACCACAGCAATCATCCAGACCTTTCTGATCC
ATCCGTCATTTCCAGTAGACATTCGCCATAACGCAAAGATTTTTCGCGAACAACTGGCCGATTGGGCCGCACGTCGCCTG
TGTATACCTGTGTGA

Upstream 100 bases:

>100_bases
GGTCATTCGGGGCGGTTCGCCCGACTCGAAGAGTTTGCCTGGGAGTATGCGTTTATCTTAACCGCATTGGGCATCCATGC
GTAGAGAAGCAGAGGTCAGG

Downstream 100 bases:

>100_bases
AAAAGCTATGATTGCATTAGTTACCGGGGGGAATGGGTTTGTCGGTCGTTACATCGTCGAGCAACTGCTGGCACGTGGTG
ATCATGTGCGCGTGATTGGA

Product: peptide synthase

Products: AMP; diphosphate +an acyl-CoA [C]

Alternate protein names: NA

Number of amino acids: Translated: 564; Mature: 564

Protein sequence:

>564_residues
MRNEEQTAARVTFTVVAGANVARHLPLMARERPDQTAVVSGVGRDRAGKVIYRRQSFAGLHAASDRLAWGLTAYGLRKGM
RVLLMVPAGVPLISLTFALMKAGCVPILIDPAMGRRNLAQCIAEVEPEAFIGVPRAHLLRLIFPRSCATIRYAVSVGPAL
PGAAALRELDIPMKTAFPLADMRSDDPAAIVFTSGSTGTPKGVLYTHGMFEAQIHTLRDLFGIAAGEVEMPAFPLFALFN
VALGVTSAIPPIDPTRPAQCDPAAVVEFIRDLGVTSTFGSPAIWEKVTAYCLTHGIQLPSLRRVLMAGAPVPIHLHERLH
RILSPTADSFTPYGATEALPVTSISGREVLAAHTEYPSPLAGTCIGYPVPGVEVAIIPITDEPIHAWQDTHRLPPGVIGE
ICVSGATVTHTYVGRPQATALAKISDGNRIWHRMGDLGYFDERGRLWFYGRKSQRVITAHGTLFTEPVERLFNQHPAVAR
SALVGIGKPGTQLPVVVVERRRDRAISTSQLIRELRQLATTTETTAIIQTFLIHPSFPVDIRHNAKIFREQLADWAARRL
CIPV

Sequences:

>Translated_564_residues
MRNEEQTAARVTFTVVAGANVARHLPLMARERPDQTAVVSGVGRDRAGKVIYRRQSFAGLHAASDRLAWGLTAYGLRKGM
RVLLMVPAGVPLISLTFALMKAGCVPILIDPAMGRRNLAQCIAEVEPEAFIGVPRAHLLRLIFPRSCATIRYAVSVGPAL
PGAAALRELDIPMKTAFPLADMRSDDPAAIVFTSGSTGTPKGVLYTHGMFEAQIHTLRDLFGIAAGEVEMPAFPLFALFN
VALGVTSAIPPIDPTRPAQCDPAAVVEFIRDLGVTSTFGSPAIWEKVTAYCLTHGIQLPSLRRVLMAGAPVPIHLHERLH
RILSPTADSFTPYGATEALPVTSISGREVLAAHTEYPSPLAGTCIGYPVPGVEVAIIPITDEPIHAWQDTHRLPPGVIGE
ICVSGATVTHTYVGRPQATALAKISDGNRIWHRMGDLGYFDERGRLWFYGRKSQRVITAHGTLFTEPVERLFNQHPAVAR
SALVGIGKPGTQLPVVVVERRRDRAISTSQLIRELRQLATTTETTAIIQTFLIHPSFPVDIRHNAKIFREQLADWAARRL
CIPV
>Mature_564_residues
MRNEEQTAARVTFTVVAGANVARHLPLMARERPDQTAVVSGVGRDRAGKVIYRRQSFAGLHAASDRLAWGLTAYGLRKGM
RVLLMVPAGVPLISLTFALMKAGCVPILIDPAMGRRNLAQCIAEVEPEAFIGVPRAHLLRLIFPRSCATIRYAVSVGPAL
PGAAALRELDIPMKTAFPLADMRSDDPAAIVFTSGSTGTPKGVLYTHGMFEAQIHTLRDLFGIAAGEVEMPAFPLFALFN
VALGVTSAIPPIDPTRPAQCDPAAVVEFIRDLGVTSTFGSPAIWEKVTAYCLTHGIQLPSLRRVLMAGAPVPIHLHERLH
RILSPTADSFTPYGATEALPVTSISGREVLAAHTEYPSPLAGTCIGYPVPGVEVAIIPITDEPIHAWQDTHRLPPGVIGE
ICVSGATVTHTYVGRPQATALAKISDGNRIWHRMGDLGYFDERGRLWFYGRKSQRVITAHGTLFTEPVERLFNQHPAVAR
SALVGIGKPGTQLPVVVVERRRDRAISTSQLIRELRQLATTTETTAIIQTFLIHPSFPVDIRHNAKIFREQLADWAARRL
CIPV

Specific function: Esterification, Concomitant With Transport, Of Exogenous Long-Chain Fatty Acids Into Metabolically Active CoA Thioesters For Subsequent Degradation Or Incorporation Into Phospholipids. [C]

COG id: COG0318

COG function: function code IQ; Acyl-CoA synthetases (AMP-forming)/AMP-acid ligases II

Gene ontology:

Cell location: Partially Membrane-Associated [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family [H]

Homologues:

Organism=Escherichia coli, GI1788107, Length=558, Percent_Identity=24.0143369175627, Blast_Score=108, Evalue=9e-25,
Organism=Escherichia coli, GI145693145, Length=501, Percent_Identity=24.3512974051896, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI221142682, Length=497, Percent_Identity=24.5472837022133, Blast_Score=91, Evalue=2e-19,
Organism=Escherichia coli, GI1786801, Length=424, Percent_Identity=23.1132075471698, Blast_Score=77, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI71994690, Length=475, Percent_Identity=24, Blast_Score=106, Evalue=3e-23,
Organism=Caenorhabditis elegans, GI71994703, Length=475, Percent_Identity=24, Blast_Score=106, Evalue=3e-23,
Organism=Caenorhabditis elegans, GI71994694, Length=475, Percent_Identity=24, Blast_Score=106, Evalue=4e-23,
Organism=Caenorhabditis elegans, GI17560308, Length=502, Percent_Identity=24.1035856573705, Blast_Score=98, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI17558820, Length=472, Percent_Identity=20.5508474576271, Blast_Score=82, Evalue=1e-15,
Organism=Drosophila melanogaster, GI24648253, Length=320, Percent_Identity=24.375, Blast_Score=73, Evalue=4e-13,
Organism=Drosophila melanogaster, GI24648255, Length=320, Percent_Identity=24.375, Blast_Score=72, Evalue=9e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020845
- InterPro:   IPR000873 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: 6.2.1.3 [C]

Molecular weight: Translated: 61263; Mature: 61263

Theoretical pI: Translated: 9.52; Mature: 9.52

Prosite motif: PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRNEEQTAARVTFTVVAGANVARHLPLMARERPDQTAVVSGVGRDRAGKVIYRRQSFAGL
CCCCHHHHEEEEEEEEECCCHHHHCCHHHCCCCCCHHHHCCCCCCCCCCEEEEEHHHCCC
HAASDRLAWGLTAYGLRKGMRVLLMVPAGVPLISLTFALMKAGCVPILIDPAMGRRNLAQ
HHCCCHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHHCCCEEEEECCCCCHHHHHH
CIAEVEPEAFIGVPRAHLLRLIFPRSCATIRYAVSVGPALPGAAALRELDIPMKTAFPLA
HHHHCCCHHEECCCHHHHHHHHCCCCCCEEEEEEECCCCCCCHHHHHHCCCCHHHCCCHH
DMRSDDPAAIVFTSGSTGTPKGVLYTHGMFEAQIHTLRDLFGIAAGEVEMPAFPLFALFN
HCCCCCCCEEEEECCCCCCCCCEEEECCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHH
VALGVTSAIPPIDPTRPAQCDPAAVVEFIRDLGVTSTFGSPAIWEKVTAYCLTHGIQLPS
HHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCCHH
LRRVLMAGAPVPIHLHERLHRILSPTADSFTPYGATEALPVTSISGREVLAAHTEYPSPL
HHHHHHCCCCCEEHHHHHHHHHHCCCCCCCCCCCCCCCCEECCCCCCEEEEECCCCCCCC
AGTCIGYPVPGVEVAIIPITDEPIHAWQDTHRLPPGVIGEICVSGATVTHTYVGRPQATA
CCCCCCCCCCCCEEEEEEECCCCHHHHHHHCCCCCCHHHHHHHCCCEEEEEECCCCCCEE
LAKISDGNRIWHRMGDLGYFDERGRLWFYGRKSQRVITAHGTLFTEPVERLFNQHPAVAR
EEEECCCCHHHHHHCCCCCCCCCCCEEEEECCCCEEEEECCCCHHHHHHHHHHCCCHHHH
SALVGIGKPGTQLPVVVVERRRDRAISTSQLIRELRQLATTTETTAIIQTFLIHPSFPVD
HHHHCCCCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
IRHNAKIFREQLADWAARRLCIPV
CCCCHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MRNEEQTAARVTFTVVAGANVARHLPLMARERPDQTAVVSGVGRDRAGKVIYRRQSFAGL
CCCCHHHHEEEEEEEEECCCHHHHCCHHHCCCCCCHHHHCCCCCCCCCCEEEEEHHHCCC
HAASDRLAWGLTAYGLRKGMRVLLMVPAGVPLISLTFALMKAGCVPILIDPAMGRRNLAQ
HHCCCHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHHCCCEEEEECCCCCHHHHHH
CIAEVEPEAFIGVPRAHLLRLIFPRSCATIRYAVSVGPALPGAAALRELDIPMKTAFPLA
HHHHCCCHHEECCCHHHHHHHHCCCCCCEEEEEEECCCCCCCHHHHHHCCCCHHHCCCHH
DMRSDDPAAIVFTSGSTGTPKGVLYTHGMFEAQIHTLRDLFGIAAGEVEMPAFPLFALFN
HCCCCCCCEEEEECCCCCCCCCEEEECCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHH
VALGVTSAIPPIDPTRPAQCDPAAVVEFIRDLGVTSTFGSPAIWEKVTAYCLTHGIQLPS
HHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCCHH
LRRVLMAGAPVPIHLHERLHRILSPTADSFTPYGATEALPVTSISGREVLAAHTEYPSPL
HHHHHHCCCCCEEHHHHHHHHHHCCCCCCCCCCCCCCCCEECCCCCCEEEEECCCCCCCC
AGTCIGYPVPGVEVAIIPITDEPIHAWQDTHRLPPGVIGEICVSGATVTHTYVGRPQATA
CCCCCCCCCCCCEEEEEEECCCCHHHHHHHCCCCCCHHHHHHHCCCEEEEEECCCCCCEE
LAKISDGNRIWHRMGDLGYFDERGRLWFYGRKSQRVITAHGTLFTEPVERLFNQHPAVAR
EEEECCCCHHHHHHCCCCCCCCCCCEEEEECCCCEEEEECCCCHHHHHHHHHHCCCHHHH
SALVGIGKPGTQLPVVVVERRRDRAISTSQLIRELRQLATTTETTAIIQTFLIHPSFPVD
HHHHCCCCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
IRHNAKIFREQLADWAARRLCIPV
CCCCHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Salts [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.0408 {octanoate}} 0.0125 {octanoate}} 0.0059 {octanoate}} 0.00072 {octanoate}} 0.0013 {undecanoate}} 0.0022 {pentadecanoate}} 0.053 {CoA}} 0.035 {CoA}} 0.034 {CoA}} 0.0034 {tridecanoate}} 0.002 {laurate}} 0.0019 {laurate}} 0.0

Substrates: ATP; a long-chain carboxylic acid; CoA [C]

Specific reaction: ATP + a long-chain carboxylic acid + CoA = AMP + diphosphate +an acyl-CoA [C]

General reaction: Acid-thiol ligation; Phosphorylation [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]