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The map label for this gene is ybjS [C]

Identifier: 222527028

GI number: 222527028

Start: 4738821

End: 4739816

Strand: Reverse

Name: ybjS [C]

Synonym: Chy400_3807

Alternate gene names: 222527028

Gene position: 4739816-4738821 (Counterclockwise)

Preceding gene: 222527029

Following gene: 222527027

Centisome position: 89.96

GC content: 58.03

Gene sequence:

>996_bases
ATGATTGCATTAGTTACCGGGGGGAATGGGTTTGTCGGTCGTTACATCGTCGAGCAACTGCTGGCACGTGGTGATCATGT
GCGCGTGATTGGACGTGGTGCCTATCCTGAATTGCAATCACTGGGGGCTGAGACCTATCAGGCCGACCTGACCTTACCTG
AGTCGGCGCCGGTGCTGGCACGGGCAATGCGCGGGGTAACGACCGTATTTCACGTTGCTGCGAAAGCCGGATTATGGGGT
AGCTACGACGACTTCTACCGGGCCAACGTCAGTGCCACCCAGCGGGTGGTCAAGGCGGCCATTCGGGCCGGCGTGCCAAA
ACTGGTCTATACCTCAACCCCTTCGGTTGTGATTGGACACGAGGACATCCACGGTGGGGACGAACACCTGCCATACCCCA
GGCGGTATCTTGCACCGTATCCGCACACCAAGGCGATTGCCGAACGGTACGTGCTTGCCCAGACCGACATTGCGACGGTT
TCGTTGCGTCCACATCTGATCTGGGGGCCACGCGATCCCCACATTCTGCCGCGGCTGCTCCGACGCGCACGACGGCGAAT
GCTCTTCCAAATCGGTGATGGAACAAATCTAGTTGACGTATGTTACGTCGAAAATGCTGCCACTGCGCACATCCAGGCAG
CTTCAGCGCTGAACGAACGCTCGCCGCTCCGTGGTCGCGCCTACTTCATTGGTCAGGAACGTCCGGTCAACCTCTGGCAA
TTTATCGGCGAAATTCTGAAAGCGGCCAACTGTCCACCGGTACGCGGTCGGATATCAGCGTCAGCAGCAACAATCCTGGC
TACCGGCTTAGAATTGCTTTACACCATACTCCGCCTTCCCGGCGAGCCACCACTCACGCGCCTGATGGTACACGAACTCA
GCCATTCGCACTGGTTTAGCCATGCTGCTGCTGAACGAGATTTTGGGTACACGCCGCGGATCAGCATTGAGGAAGGTTTA
GAGCGCACGTTTGCCCTGACAGGCAGCCAGCCGTAA

Upstream 100 bases:

>100_bases
CCGTCATTTCCAGTAGACATTCGCCATAACGCAAAGATTTTTCGCGAACAACTGGCCGATTGGGCCGCACGTCGCCTGTG
TATACCTGTGTGAAAAAGCT

Downstream 100 bases:

>100_bases
ACGAGAGCACCGTTTCAGCAATCTCCAATCATTACCGAACAGGGAGGATTTCGTGCTATTCAGGCATGTCATGATCGAAG
CAGTCAGTTATGTGTTAGCT

Product: 3-beta hydroxysteroid dehydrogenase/isomerase

Products: NA

Alternate protein names: Cholesterol dehydrogenase; 3-beta-hydroxy-Delta(5)-steroid dehydrogenase; 3-beta-HSD; 3BHSD; 3-beta hydroxysterol dehydrogenase; 3-beta-hydroxy-5-ene steroid dehydrogenase; Progesterone reductase; Steroid Delta-isomerase; Delta-5-3-ketosteroid isomerase [H]

Number of amino acids: Translated: 331; Mature: 331

Protein sequence:

>331_residues
MIALVTGGNGFVGRYIVEQLLARGDHVRVIGRGAYPELQSLGAETYQADLTLPESAPVLARAMRGVTTVFHVAAKAGLWG
SYDDFYRANVSATQRVVKAAIRAGVPKLVYTSTPSVVIGHEDIHGGDEHLPYPRRYLAPYPHTKAIAERYVLAQTDIATV
SLRPHLIWGPRDPHILPRLLRRARRRMLFQIGDGTNLVDVCYVENAATAHIQAASALNERSPLRGRAYFIGQERPVNLWQ
FIGEILKAANCPPVRGRISASAATILATGLELLYTILRLPGEPPLTRLMVHELSHSHWFSHAAAERDFGYTPRISIEEGL
ERTFALTGSQP

Sequences:

>Translated_331_residues
MIALVTGGNGFVGRYIVEQLLARGDHVRVIGRGAYPELQSLGAETYQADLTLPESAPVLARAMRGVTTVFHVAAKAGLWG
SYDDFYRANVSATQRVVKAAIRAGVPKLVYTSTPSVVIGHEDIHGGDEHLPYPRRYLAPYPHTKAIAERYVLAQTDIATV
SLRPHLIWGPRDPHILPRLLRRARRRMLFQIGDGTNLVDVCYVENAATAHIQAASALNERSPLRGRAYFIGQERPVNLWQ
FIGEILKAANCPPVRGRISASAATILATGLELLYTILRLPGEPPLTRLMVHELSHSHWFSHAAAERDFGYTPRISIEEGL
ERTFALTGSQP
>Mature_331_residues
MIALVTGGNGFVGRYIVEQLLARGDHVRVIGRGAYPELQSLGAETYQADLTLPESAPVLARAMRGVTTVFHVAAKAGLWG
SYDDFYRANVSATQRVVKAAIRAGVPKLVYTSTPSVVIGHEDIHGGDEHLPYPRRYLAPYPHTKAIAERYVLAQTDIATV
SLRPHLIWGPRDPHILPRLLRRARRRMLFQIGDGTNLVDVCYVENAATAHIQAASALNERSPLRGRAYFIGQERPVNLWQ
FIGEILKAANCPPVRGRISASAATILATGLELLYTILRLPGEPPLTRLMVHELSHSHWFSHAAAERDFGYTPRISIEEGL
ERTFALTGSQP

Specific function: 3-beta-HSD is a bifunctional enzyme, that catalyzes the oxidation and isomerization of cholesterol, pregnenolone, and dehydroepiandrosterone (DHEA) into cholest-4-en-3-one, progesterone, and androsterone, respectively [H]

COG id: COG0451

COG function: function code MG; Nucleoside-diphosphate-sugar epimerases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 3-beta-HSD family [H]

Homologues:

Organism=Homo sapiens, GI193211614, Length=330, Percent_Identity=35.7575757575758, Blast_Score=183, Evalue=2e-46,
Organism=Homo sapiens, GI8393516, Length=330, Percent_Identity=35.7575757575758, Blast_Score=183, Evalue=2e-46,
Organism=Homo sapiens, GI116268111, Length=341, Percent_Identity=31.0850439882698, Blast_Score=125, Evalue=4e-29,
Organism=Homo sapiens, GI4504507, Length=354, Percent_Identity=28.5310734463277, Blast_Score=118, Evalue=8e-27,
Organism=Homo sapiens, GI239745448, Length=333, Percent_Identity=32.1321321321321, Blast_Score=117, Evalue=2e-26,
Organism=Homo sapiens, GI310132178, Length=333, Percent_Identity=31.8318318318318, Blast_Score=114, Evalue=1e-25,
Organism=Homo sapiens, GI310113012, Length=333, Percent_Identity=31.8318318318318, Blast_Score=114, Evalue=1e-25,
Organism=Homo sapiens, GI260763931, Length=354, Percent_Identity=28.2485875706215, Blast_Score=112, Evalue=4e-25,
Organism=Homo sapiens, GI4504509, Length=354, Percent_Identity=28.2485875706215, Blast_Score=112, Evalue=4e-25,
Organism=Homo sapiens, GI19923621, Length=373, Percent_Identity=29.2225201072386, Blast_Score=83, Evalue=5e-16,
Organism=Escherichia coli, GI87081792, Length=341, Percent_Identity=30.2052785923754, Blast_Score=115, Evalue=4e-27,
Organism=Caenorhabditis elegans, GI17570557, Length=326, Percent_Identity=26.9938650306748, Blast_Score=97, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI193211272, Length=340, Percent_Identity=23.8235294117647, Blast_Score=92, Evalue=3e-19,
Organism=Caenorhabditis elegans, GI17509709, Length=271, Percent_Identity=27.3062730627306, Blast_Score=71, Evalue=8e-13,
Organism=Saccharomyces cerevisiae, GI6321437, Length=345, Percent_Identity=30.7246376811594, Blast_Score=141, Evalue=2e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: =1.1.1.145; =5.3.3.1 [H]

Molecular weight: Translated: 36506; Mature: 36506

Theoretical pI: Translated: 9.67; Mature: 9.67

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIALVTGGNGFVGRYIVEQLLARGDHVRVIGRGAYPELQSLGAETYQADLTLPESAPVLA
CEEEEECCCCCHHHHHHHHHHHCCCEEEEEECCCCHHHHHCCCCCEEEEEECCCCCHHHH
RAMRGVTTVFHVAAKAGLWGSYDDFYRANVSATQRVVKAAIRAGVPKLVYTSTPSVVIGH
HHHHHHHHHHHHHHHCCCCCCCHHHHHCCHHHHHHHHHHHHHHCCCCEEEECCCCEEECC
EDIHGGDEHLPYPRRYLAPYPHTKAIAERYVLAQTDIATVSLRPHLIWGPRDPHILPRLL
CCCCCCCCCCCCCHHHCCCCCCHHHHHHHHHHEECCEEEEEECCEEEECCCCCCHHHHHH
RRARRRMLFQIGDGTNLVDVCYVENAATAHIQAASALNERSPLRGRAYFIGQERPVNLWQ
HHHHHHHEEEECCCCCEEEEEEECCCCHHHHHHHHHHCCCCCCCCCEEEECCCCCCHHHH
FIGEILKAANCPPVRGRISASAATILATGLELLYTILRLPGEPPLTRLMVHELSHSHWFS
HHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHH
HAAAERDFGYTPRISIEEGLERTFALTGSQP
HHHHCCCCCCCCCCCHHHHHHHEEEECCCCC
>Mature Secondary Structure
MIALVTGGNGFVGRYIVEQLLARGDHVRVIGRGAYPELQSLGAETYQADLTLPESAPVLA
CEEEEECCCCCHHHHHHHHHHHCCCEEEEEECCCCHHHHHCCCCCEEEEEECCCCCHHHH
RAMRGVTTVFHVAAKAGLWGSYDDFYRANVSATQRVVKAAIRAGVPKLVYTSTPSVVIGH
HHHHHHHHHHHHHHHCCCCCCCHHHHHCCHHHHHHHHHHHHHHCCCCEEEECCCCEEECC
EDIHGGDEHLPYPRRYLAPYPHTKAIAERYVLAQTDIATVSLRPHLIWGPRDPHILPRLL
CCCCCCCCCCCCCHHHCCCCCCHHHHHHHHHHEECCEEEEEECCEEEECCCCCCHHHHHH
RRARRRMLFQIGDGTNLVDVCYVENAATAHIQAASALNERSPLRGRAYFIGQERPVNLWQ
HHHHHHHEEEECCCCCEEEEEEECCCCHHHHHHHHHHCCCCCCCCCEEEECCCCCCHHHH
FIGEILKAANCPPVRGRISASAATILATGLELLYTILRLPGEPPLTRLMVHELSHSHWFS
HHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHH
HAAAERDFGYTPRISIEEGLERTFALTGSQP
HHHHCCCCCCCCCCCHHHHHHHEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]