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The map label for this gene is phoB [H]

Identifier: 222526562

GI number: 222526562

Start: 4151381

End: 4152694

Strand: Reverse

Name: phoB [H]

Synonym: Chy400_3330

Alternate gene names: 222526562

Gene position: 4152694-4151381 (Counterclockwise)

Preceding gene: 222526570

Following gene: 222526556

Centisome position: 78.81

GC content: 54.03

Gene sequence:

>1314_bases
ATGACAGCACCGGCTCGCATTCTCGTTGCCGAAGATGAACCAGATGTGGGCATGCTCCTGCGGGAAATTCTAGTAAGCGA
AGGGTACGAGGTAGAACTGGTACCTAATGGCCACCTCCTGGTGGAACGCGCCCAGGAACAGCCACCGGATTTGATCCTGG
TTGATCTGCTCATGCCGTTGATGGATGGCCTGGAGGCAATCCGCCAACTGCGTAACGACACCCGCACGTCACACCTGCCG
ATGCTCATCCTCACCGCACTTGGCGAGTCGTCGCGCATTGTTGAAGGATTGGAGACCGGTGCCGATGACTATATTGTCAA
ACCTTATGAACGCGAAGTATTGCTGGCCCGTGTACGCTCACAATTGCGACGAGCATCGCAATTACCGGCCCGGAATCCGC
TCACCGGCTTACCCGGCAATGTCTCCATTCAGGCCGAGATCAATCGTCAACTCCAGCAAGGGGCTAAATTTGCCCTGCTG
TATATCGATCTCGATAATTTCAAAGCGTTCAATGATGTTTATGGGTTTGCACGTGGCGACAAGGCGCTACACCTCGTGGC
CAGTGTCTTACAGGAACAGTGCCCTTCGGGAGACTTTATCGGTCATATCGGTGGCGACGATTTTGTGGTGATTCACTTCG
GTGCTGACCCTGAGACGTTGTGCAAGCGTATTATCCAGGTCTTTGACCAGCGTGTTCGTACCCTGTACGACGAGGCAGAT
CTCCAACGGGGCTATCTGGTTGCCACAGATCGCTACGGTATTGTGCGTCAGTTTGGAATTATTAGCCTTTCTATCGCAGT
GGTAACGACCTACACACGCAGCTTTCGCAATTATGACGAAATAGGGCGGGTTGCGGCGGAATTGAAGCAGGCGGCCAAGC
AGATTGCCGGCAGTGCGTATAAGATAGATCGGCGCAGTGATGATGCAATCCCCCCCTCTCTCAACGACCGCCGCAGTGGT
TCATCACCCGAAGCGCTTATCGTCTGTCATAATGACATTGTGCGCGCAGCAATTATTGCTACGCTCAATGTACGTGGCTA
TCGCCTGCTCATTGCCGATAACGAGATCGCAGCCCAGGGATTACTCGCTCACAATCCCCATCCGGCGCTTATTGTTGCTG
AGTTTCAGCGATCCACACCAATTCAGATCTGGCAACAAGTGAATCACAATTCCACGCTGATTGCGTTGATCAACGATGAC
GAGGATGCCACCATCGCCCAGCAGGCTGGGGCGCAGGTTATCATTCGCCTGGACGGCAGTCCCCTCAATCTCTCTGATCA
GTTGCAGGCGGCGCTGGCGACACTGGCACGATGA

Upstream 100 bases:

>100_bases
ACGGATCACCAGTGTGTGTGGTATCCTATAGTCATCAGAAAGATTTCGCTTTCATTGGGCATCTGCTCATATGATTCATA
TAATGCGGTAGGAGCGTGAC

Downstream 100 bases:

>100_bases
GGCAGTCGTTCCTGGCCGGTGCTGCTCCGGCGGGTTAAAACCCTGCCTCAAGCCACACCAGCCCCGGTGGGCTGGCCTGC
CGGCGGAGTGTGGGGGCCTC

Product: response regulator receiver modulated diguanylate cyclase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 437; Mature: 436

Protein sequence:

>437_residues
MTAPARILVAEDEPDVGMLLREILVSEGYEVELVPNGHLLVERAQEQPPDLILVDLLMPLMDGLEAIRQLRNDTRTSHLP
MLILTALGESSRIVEGLETGADDYIVKPYEREVLLARVRSQLRRASQLPARNPLTGLPGNVSIQAEINRQLQQGAKFALL
YIDLDNFKAFNDVYGFARGDKALHLVASVLQEQCPSGDFIGHIGGDDFVVIHFGADPETLCKRIIQVFDQRVRTLYDEAD
LQRGYLVATDRYGIVRQFGIISLSIAVVTTYTRSFRNYDEIGRVAAELKQAAKQIAGSAYKIDRRSDDAIPPSLNDRRSG
SSPEALIVCHNDIVRAAIIATLNVRGYRLLIADNEIAAQGLLAHNPHPALIVAEFQRSTPIQIWQQVNHNSTLIALINDD
EDATIAQQAGAQVIIRLDGSPLNLSDQLQAALATLAR

Sequences:

>Translated_437_residues
MTAPARILVAEDEPDVGMLLREILVSEGYEVELVPNGHLLVERAQEQPPDLILVDLLMPLMDGLEAIRQLRNDTRTSHLP
MLILTALGESSRIVEGLETGADDYIVKPYEREVLLARVRSQLRRASQLPARNPLTGLPGNVSIQAEINRQLQQGAKFALL
YIDLDNFKAFNDVYGFARGDKALHLVASVLQEQCPSGDFIGHIGGDDFVVIHFGADPETLCKRIIQVFDQRVRTLYDEAD
LQRGYLVATDRYGIVRQFGIISLSIAVVTTYTRSFRNYDEIGRVAAELKQAAKQIAGSAYKIDRRSDDAIPPSLNDRRSG
SSPEALIVCHNDIVRAAIIATLNVRGYRLLIADNEIAAQGLLAHNPHPALIVAEFQRSTPIQIWQQVNHNSTLIALINDD
EDATIAQQAGAQVIIRLDGSPLNLSDQLQAALATLAR
>Mature_436_residues
TAPARILVAEDEPDVGMLLREILVSEGYEVELVPNGHLLVERAQEQPPDLILVDLLMPLMDGLEAIRQLRNDTRTSHLPM
LILTALGESSRIVEGLETGADDYIVKPYEREVLLARVRSQLRRASQLPARNPLTGLPGNVSIQAEINRQLQQGAKFALLY
IDLDNFKAFNDVYGFARGDKALHLVASVLQEQCPSGDFIGHIGGDDFVVIHFGADPETLCKRIIQVFDQRVRTLYDEADL
QRGYLVATDRYGIVRQFGIISLSIAVVTTYTRSFRNYDEIGRVAAELKQAAKQIAGSAYKIDRRSDDAIPPSLNDRRSGS
SPEALIVCHNDIVRAAIIATLNVRGYRLLIADNEIAAQGLLAHNPHPALIVAEFQRSTPIQIWQQVNHNSTLIALINDDE
DATIAQQAGAQVIIRLDGSPLNLSDQLQAALATLAR

Specific function: This protein is a positive regulator for the phosphate regulon. Transcription of this operon is positively regulated by phoB and phoR when phosphate is limited [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1788394, Length=121, Percent_Identity=42.9752066115703, Blast_Score=94, Evalue=2e-20,
Organism=Escherichia coli, GI1786784, Length=121, Percent_Identity=41.3223140495868, Blast_Score=89, Evalue=5e-19,
Organism=Escherichia coli, GI1786599, Length=123, Percent_Identity=38.2113821138211, Blast_Score=86, Evalue=4e-18,
Organism=Escherichia coli, GI1788550, Length=111, Percent_Identity=36.9369369369369, Blast_Score=80, Evalue=3e-16,
Organism=Escherichia coli, GI1790860, Length=128, Percent_Identity=35.9375, Blast_Score=79, Evalue=6e-16,
Organism=Escherichia coli, GI1789809, Length=126, Percent_Identity=38.0952380952381, Blast_Score=79, Evalue=6e-16,
Organism=Escherichia coli, GI1786911, Length=131, Percent_Identity=39.6946564885496, Blast_Score=76, Evalue=3e-15,
Organism=Escherichia coli, GI87082012, Length=132, Percent_Identity=34.8484848484849, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI1787229, Length=123, Percent_Identity=39.0243902439024, Blast_Score=72, Evalue=8e-14,
Organism=Escherichia coli, GI1790437, Length=127, Percent_Identity=33.0708661417323, Blast_Score=69, Evalue=6e-13,
Organism=Escherichia coli, GI1790552, Length=122, Percent_Identity=36.0655737704918, Blast_Score=67, Evalue=3e-12,
Organism=Escherichia coli, GI1787375, Length=126, Percent_Identity=34.9206349206349, Blast_Score=66, Evalue=4e-12,
Organism=Escherichia coli, GI1789402, Length=121, Percent_Identity=34.7107438016529, Blast_Score=66, Evalue=5e-12,
Organism=Escherichia coli, GI2367329, Length=120, Percent_Identity=36.6666666666667, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR001867
- InterPro:   IPR016032
- InterPro:   IPR011879
- InterPro:   IPR001789
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00072 Response_reg; PF00486 Trans_reg_C [H]

EC number: NA

Molecular weight: Translated: 48228; Mature: 48097

Theoretical pI: Translated: 4.98; Mature: 4.98

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS00152 ATPASE_ALPHA_BETA ; PS50887 GGDEF ; PS00217 SUGAR_TRANSPORT_2 ; PS00237 G_PROTEIN_RECEP_F1_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAPARILVAEDEPDVGMLLREILVSEGYEVELVPNGHLLVERAQEQPPDLILVDLLMPL
CCCCCEEEEECCCCCHHHHHHHHHHCCCCEEEEECCCCEEEEEHHCCCCCEEHHHHHHHH
MDGLEAIRQLRNDTRTSHLPMLILTALGESSRIVEGLETGADDYIVKPYEREVLLARVRS
HHHHHHHHHHHHCCCCCCCHHHHEECCCCCHHHHHHHHCCCCCCEECCHHHHHHHHHHHH
QLRRASQLPARNPLTGLPGNVSIQAEINRQLQQGAKFALLYIDLDNFKAFNDVYGFARGD
HHHHHHCCCCCCCCCCCCCCEEEEHHHHHHHHCCCCEEEEEEECCCCHHHHHHHHHCCCC
KALHLVASVLQEQCPSGDFIGHIGGDDFVVIHFGADPETLCKRIIQVFDQRVRTLYDEAD
HHHHHHHHHHHHHCCCCCEEEECCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHH
LQRGYLVATDRYGIVRQFGIISLSIAVVTTYTRSFRNYDEIGRVAAELKQAAKQIAGSAY
CCCCEEEEECCCHHHHHHHHHEEHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCE
KIDRRSDDAIPPSLNDRRSGSSPEALIVCHNDIVRAAIIATLNVRGYRLLIADNEIAAQG
EECCCCCCCCCCCCCCCCCCCCCCEEEEECCHHHHHHHHHEEECCCEEEEEECCCHHHCC
LLAHNPHPALIVAEFQRSTPIQIWQQVNHNSTLIALINDDEDATIAQQAGAQVIIRLDGS
CCCCCCCCEEEEEECCCCCHHHHHHHCCCCCEEEEEEECCCCHHHHHHCCCEEEEEECCC
PLNLSDQLQAALATLAR
CCCHHHHHHHHHHHHCC
>Mature Secondary Structure 
TAPARILVAEDEPDVGMLLREILVSEGYEVELVPNGHLLVERAQEQPPDLILVDLLMPL
CCCCEEEEECCCCCHHHHHHHHHHCCCCEEEEECCCCEEEEEHHCCCCCEEHHHHHHHH
MDGLEAIRQLRNDTRTSHLPMLILTALGESSRIVEGLETGADDYIVKPYEREVLLARVRS
HHHHHHHHHHHHCCCCCCCHHHHEECCCCCHHHHHHHHCCCCCCEECCHHHHHHHHHHHH
QLRRASQLPARNPLTGLPGNVSIQAEINRQLQQGAKFALLYIDLDNFKAFNDVYGFARGD
HHHHHHCCCCCCCCCCCCCCEEEEHHHHHHHHCCCCEEEEEEECCCCHHHHHHHHHCCCC
KALHLVASVLQEQCPSGDFIGHIGGDDFVVIHFGADPETLCKRIIQVFDQRVRTLYDEAD
HHHHHHHHHHHHHCCCCCEEEECCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHH
LQRGYLVATDRYGIVRQFGIISLSIAVVTTYTRSFRNYDEIGRVAAELKQAAKQIAGSAY
CCCCEEEEECCCHHHHHHHHHEEHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCE
KIDRRSDDAIPPSLNDRRSGSSPEALIVCHNDIVRAAIIATLNVRGYRLLIADNEIAAQG
EECCCCCCCCCCCCCCCCCCCCCCEEEEECCHHHHHHHHHEEECCCEEEEEECCCHHHCC
LLAHNPHPALIVAEFQRSTPIQIWQQVNHNSTLIALINDDEDATIAQQAGAQVIIRLDGS
CCCCCCCCEEEEEECCCCCHHHHHHHCCCCCEEEEEEECCCCHHHHHHCCCEEEEEECCC
PLNLSDQLQAALATLAR
CCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11481430 [H]