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The map label for this gene is tyrS [H]

Identifier: 222526556

GI number: 222526556

Start: 4141082

End: 4142254

Strand: Reverse

Name: tyrS [H]

Synonym: Chy400_3324

Alternate gene names: 222526556

Gene position: 4142254-4141082 (Counterclockwise)

Preceding gene: 222526562

Following gene: 222526551

Centisome position: 78.62

GC content: 57.54

Gene sequence:

>1173_bases
ATGGATCTGTCCGAACTACTACGGCGCGGCGTTGCCGAAGTGATCGTCGAGAGCGAACTACGCGCTCGCTTGCAAAGTGG
AACGCCGCTGAGGCTAAAACAGGGCTTTGATCCCACCAAGCCCGATATGCACATCGGCCATGCCGTTGGTCTGCGCAAAT
TGCGTGCTTTCCAGGAATTGGGGCACCAGGTGGTGTTGATTGTTGGTGATTGGACGGCACAAATCGGCGATCCGAGCGGT
CGCGACGAAACCCGTACCCGCCTGTCGGCTGCTGAAGTACGGGCCAACGCCGAGACCTACATGGAGCAGTTTTTCCGCGT
GGTTGATCGCCAACGCACCGAGGTGCGCTGGCAGAGTGAATGGTTTGGGCAATTCACGCTGGAGCATGCCCTCGATCTGG
CCGGTCGCTTCACCCTGGCCCAAATGCTGGCCCACGAAACGTTCCGTAAGCGGTATGAAACCGGTGCGCCACTGACCATC
CTCGAATTGATGTATCCAATGCTTCAGGCGTATGACTCGGTGGCGATCAAGGCCGATGTAGAATTCGGTGGTACCGACCA
GAAATTCAATATCCTCGCCGGTCGTGAATTGATGGCGCAACTGGGTATGACCCCGCAACAGGTGTTCCTCGTGCCGCTCA
TTCCCGGCACCGATGGCCGTAAGATGTCGAAGACGTTTAATAACACGGTTGATATTCGGATGCCGCCATCGGAAATGTAT
GGTCGCATTATGTCAATGAGCGACGAGGTATTACCTCTCTATTTTGAAGTGCTGACCGATGTGCCGCTGGCAGAGATAGA
CGAGATGAAGCTGGCAATGGCCGCCGGGCAGGTCAATCCACGCGATCTGAAAATGCGGCTGGCGCGTGAGATTGTGGCGC
AGTTCCACGACCCGGCTGCTGCCGCTGCCGCCGAAGCTGCCTTCGTTCGTCAATTCGTTGAGCGCGAGATTCCAGAGGAT
ATTCCAACGTTTACCCTCACAGCACCGAGCGGGATAGTTGAGGTGTTGGTCGCCAGCGGTCTCGCCCCTAGCAAGAGTGA
GGCGCGCCGCCTGATTGACGGTGGTGGCGTGCGGGTTGACGGTGAACGGGTTGAGGACTACGCCCTAACCCTGTCGCCCG
GTGCTAATGTCGTGGTACAGGTTGGTCGCCGTAAGTTTGTGCGCGTTGTGTAG

Upstream 100 bases:

>100_bases
ACTCCCAACATCCGCTCGCGTTTTCCGCTTGACACACACCCCGCTCCCTGTTAGCATAAACTACGTTTGCAGTCTTCATA
AAGCGCTGGGAGCGTCTGGG

Downstream 100 bases:

>100_bases
GGGCAGGTTGTTTATCCTGCCCCTTTTGCTTCCTGGCATTGCTGTATGCCACGCTGTATCAGCACGCTGTCGCGTGCGAT
GATAGCCCGAAACCCCGCAT

Product: tyrosyl-tRNA synthetase

Products: NA

Alternate protein names: Tyrosine--tRNA ligase; TyrRS [H]

Number of amino acids: Translated: 390; Mature: 390

Protein sequence:

>390_residues
MDLSELLRRGVAEVIVESELRARLQSGTPLRLKQGFDPTKPDMHIGHAVGLRKLRAFQELGHQVVLIVGDWTAQIGDPSG
RDETRTRLSAAEVRANAETYMEQFFRVVDRQRTEVRWQSEWFGQFTLEHALDLAGRFTLAQMLAHETFRKRYETGAPLTI
LELMYPMLQAYDSVAIKADVEFGGTDQKFNILAGRELMAQLGMTPQQVFLVPLIPGTDGRKMSKTFNNTVDIRMPPSEMY
GRIMSMSDEVLPLYFEVLTDVPLAEIDEMKLAMAAGQVNPRDLKMRLAREIVAQFHDPAAAAAAEAAFVRQFVEREIPED
IPTFTLTAPSGIVEVLVASGLAPSKSEARRLIDGGGVRVDGERVEDYALTLSPGANVVVQVGRRKFVRVV

Sequences:

>Translated_390_residues
MDLSELLRRGVAEVIVESELRARLQSGTPLRLKQGFDPTKPDMHIGHAVGLRKLRAFQELGHQVVLIVGDWTAQIGDPSG
RDETRTRLSAAEVRANAETYMEQFFRVVDRQRTEVRWQSEWFGQFTLEHALDLAGRFTLAQMLAHETFRKRYETGAPLTI
LELMYPMLQAYDSVAIKADVEFGGTDQKFNILAGRELMAQLGMTPQQVFLVPLIPGTDGRKMSKTFNNTVDIRMPPSEMY
GRIMSMSDEVLPLYFEVLTDVPLAEIDEMKLAMAAGQVNPRDLKMRLAREIVAQFHDPAAAAAAEAAFVRQFVEREIPED
IPTFTLTAPSGIVEVLVASGLAPSKSEARRLIDGGGVRVDGERVEDYALTLSPGANVVVQVGRRKFVRVV
>Mature_390_residues
MDLSELLRRGVAEVIVESELRARLQSGTPLRLKQGFDPTKPDMHIGHAVGLRKLRAFQELGHQVVLIVGDWTAQIGDPSG
RDETRTRLSAAEVRANAETYMEQFFRVVDRQRTEVRWQSEWFGQFTLEHALDLAGRFTLAQMLAHETFRKRYETGAPLTI
LELMYPMLQAYDSVAIKADVEFGGTDQKFNILAGRELMAQLGMTPQQVFLVPLIPGTDGRKMSKTFNNTVDIRMPPSEMY
GRIMSMSDEVLPLYFEVLTDVPLAEIDEMKLAMAAGQVNPRDLKMRLAREIVAQFHDPAAAAAAEAAFVRQFVEREIPED
IPTFTLTAPSGIVEVLVASGLAPSKSEARRLIDGGGVRVDGERVEDYALTLSPGANVVVQVGRRKFVRVV

Specific function: Catalyzes the attachment of tyrosine to tRNA(Tyr) in a two-step reaction:tyrosine is first activated by ATP to form Tyr- AMP and then transferred to the acceptor end of tRNA(Tyr) [H]

COG id: COG0162

COG function: function code J; Tyrosyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S4 RNA-binding domain [H]

Homologues:

Organism=Homo sapiens, GI94681057, Length=429, Percent_Identity=26.1072261072261, Blast_Score=125, Evalue=5e-29,
Organism=Escherichia coli, GI1787925, Length=406, Percent_Identity=27.5862068965517, Blast_Score=128, Evalue=5e-31,
Organism=Caenorhabditis elegans, GI25145802, Length=204, Percent_Identity=25.4901960784314, Blast_Score=78, Evalue=7e-15,
Organism=Saccharomyces cerevisiae, GI6325160, Length=337, Percent_Identity=26.4094955489614, Blast_Score=97, Evalue=4e-21,
Organism=Drosophila melanogaster, GI20130383, Length=402, Percent_Identity=24.6268656716418, Blast_Score=127, Evalue=2e-29,

Paralogues:

None

Copy number: 892 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001412
- InterPro:   IPR002305
- InterPro:   IPR014729
- InterPro:   IPR002942
- InterPro:   IPR002307 [H]

Pfam domain/function: PF01479 S4; PF00579 tRNA-synt_1b [H]

EC number: =6.1.1.1 [H]

Molecular weight: Translated: 43515; Mature: 43515

Theoretical pI: Translated: 5.54; Mature: 5.54

Prosite motif: PS50889 S4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDLSELLRRGVAEVIVESELRARLQSGTPLRLKQGFDPTKPDMHIGHAVGLRKLRAFQEL
CCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCHHHHHHHHHHHHHHHHC
GHQVVLIVGDWTAQIGDPSGRDETRTRLSAAEVRANAETYMEQFFRVVDRQRTEVRWQSE
CCEEEEEEECCEEECCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHH
WFGQFTLEHALDLAGRFTLAQMLAHETFRKRYETGAPLTILELMYPMLQAYDSVAIKADV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCEEEEEEE
EFGGTDQKFNILAGRELMAQLGMTPQQVFLVPLIPGTDGRKMSKTFNNTVDIRMPPSEMY
EECCCCCEEEEHHHHHHHHHHCCCCCCEEEEEECCCCCCCHHHHHCCCEEEEEECHHHHH
GRIMSMSDEVLPLYFEVLTDVPLAEIDEMKLAMAAGQVNPRDLKMRLAREIVAQFHDPAA
HHHHHCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCHH
AAAAEAAFVRQFVEREIPEDIPTFTLTAPSGIVEVLVASGLAPSKSEARRLIDGGGVRVD
HHHHHHHHHHHHHHHHCCCCCCCEEEECCHHHHHHHHHCCCCCCHHHHHHHHCCCCEEEC
GERVEDYALTLSPGANVVVQVGRRKFVRVV
CCCCCEEEEEECCCCCEEEEECCHHHHCCC
>Mature Secondary Structure
MDLSELLRRGVAEVIVESELRARLQSGTPLRLKQGFDPTKPDMHIGHAVGLRKLRAFQEL
CCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCHHHHHHHHHHHHHHHHC
GHQVVLIVGDWTAQIGDPSGRDETRTRLSAAEVRANAETYMEQFFRVVDRQRTEVRWQSE
CCEEEEEEECCEEECCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHH
WFGQFTLEHALDLAGRFTLAQMLAHETFRKRYETGAPLTILELMYPMLQAYDSVAIKADV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCEEEEEEE
EFGGTDQKFNILAGRELMAQLGMTPQQVFLVPLIPGTDGRKMSKTFNNTVDIRMPPSEMY
EECCCCCEEEEHHHHHHHHHHCCCCCCEEEEEECCCCCCCHHHHHCCCEEEEEECHHHHH
GRIMSMSDEVLPLYFEVLTDVPLAEIDEMKLAMAAGQVNPRDLKMRLAREIVAQFHDPAA
HHHHHCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCHH
AAAAEAAFVRQFVEREIPEDIPTFTLTAPSGIVEVLVASGLAPSKSEARRLIDGGGVRVD
HHHHHHHHHHHHHHHHCCCCCCCEEEECCHHHHHHHHHCCCCCCHHHHHHHHCCCCEEEC
GERVEDYALTLSPGANVVVQVGRRKFVRVV
CCCCCEEEEEECCCCCEEEEECCHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA