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The map label for this gene is rpfG [H]

Identifier: 222526570

GI number: 222526570

Start: 4160157

End: 4161230

Strand: Reverse

Name: rpfG [H]

Synonym: Chy400_3338

Alternate gene names: 222526570

Gene position: 4161230-4160157 (Counterclockwise)

Preceding gene: 222526571

Following gene: 222526562

Centisome position: 78.98

GC content: 54.1

Gene sequence:

>1074_bases
ATGCAAAATCAACCCTATAATGTGTTGGTCATCGACGATGACCCTAACGTGCGTGATATTCTAAGTAGCCTGCTCAAACG
TGAACGCTGTGTCGTCCGCACCGCAGCATCAGGGCAAATCGGCATTGCGGCTGCCAGGTCCGAGTTACCCGATCTCATCC
TGCTTGATGTCATGATGCCCGATCTCGATGGGTTCAGTGTCTGCCGCATGTTGCGTGAAGATCCGCTGACGGCTGAAGTT
CCCATTATTATGATTACTGCGCTTGATGATCGGGCTTCGCGGCTGGAAGGGGTAAAAGCCGGGGCTGATGAGTTTTTATC
CAAGCCGGTCGAATTGAGCGAGTTGCGCCTGCGGGTGCGCACGATGCAGCGGATCAACCGCTACCGCCGATTAATTGAAG
AGCGCGAGCGCGCAGCAGCTCAGGAGCGTCTCCTGACCGAGCAAGCCCGGCAGGCAGCCCAGGCGATTGCCGAAGCGTAT
GATCAAACGCTGATCGGCTGGTCACGTGCCCTCGATCTCCGGGACAAAGAGACAGAGGGACATTCACAACGAGTCGCTCA
ACTTACGCTGGCTGTGGCGCAGGCACTCAATATTCCTCCAGAAGAGCAGATCAATATGTGGCGCGGGGCGATGCTCCATG
ATATTGGCAAAATTGGCGTACCAGACGCCATTTTGCACAAACCAGGACCACTAACCGAAGAAGAATGGGAGATTATGCGT
CGCCACACAACCTATGCCTACGAGTTACTCTACCCAATCGCTTACCTGCGCCCCGCCCTGGACATTCCATATTGCCATCA
TGAAAAATGGGATGGTACCGGCTACCCACGAGGCTTGCGCGGTGAACAGATTCCACTCGCGGCCCGTATCTTTGCCCTGG
TTGATGTTTGGGATGCGCTGACCAATGATCGACCCTACCGCAAGGCTTGGAGTCGTGAGCAAGCCTGGCAATACATTCAC
GATCAAAAGGGTAAACATTTTGATCCGCAACTGGTTGATCTTTTTCTCTCCATTGCTGCACCTGACCTGACGAAGGTGAT
GGAACGCCAGGTTTCTGGAGGGAAAGAAGATTAG

Upstream 100 bases:

>100_bases
CGAAATTTTATCAGTACGATTTGAACCCGATATACCACGTCACGCCATGCTACAATAAACATCGCAGCGACATCCGTTAA
GCCGGAAAGCATCATAGCCT

Downstream 100 bases:

>100_bases
CGGTTAGCTCTTGTTTTCAGGACAGATCACACCACTGATAGCGGGTCACGTCTTCTCTACTTCCAGATATGATGTGAAAT
ACCCCCCACCCTTCCCGCCC

Product: response regulator receiver modulated metal dependent phosphohydrolase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 357; Mature: 357

Protein sequence:

>357_residues
MQNQPYNVLVIDDDPNVRDILSSLLKRERCVVRTAASGQIGIAAARSELPDLILLDVMMPDLDGFSVCRMLREDPLTAEV
PIIMITALDDRASRLEGVKAGADEFLSKPVELSELRLRVRTMQRINRYRRLIEERERAAAQERLLTEQARQAAQAIAEAY
DQTLIGWSRALDLRDKETEGHSQRVAQLTLAVAQALNIPPEEQINMWRGAMLHDIGKIGVPDAILHKPGPLTEEEWEIMR
RHTTYAYELLYPIAYLRPALDIPYCHHEKWDGTGYPRGLRGEQIPLAARIFALVDVWDALTNDRPYRKAWSREQAWQYIH
DQKGKHFDPQLVDLFLSIAAPDLTKVMERQVSGGKED

Sequences:

>Translated_357_residues
MQNQPYNVLVIDDDPNVRDILSSLLKRERCVVRTAASGQIGIAAARSELPDLILLDVMMPDLDGFSVCRMLREDPLTAEV
PIIMITALDDRASRLEGVKAGADEFLSKPVELSELRLRVRTMQRINRYRRLIEERERAAAQERLLTEQARQAAQAIAEAY
DQTLIGWSRALDLRDKETEGHSQRVAQLTLAVAQALNIPPEEQINMWRGAMLHDIGKIGVPDAILHKPGPLTEEEWEIMR
RHTTYAYELLYPIAYLRPALDIPYCHHEKWDGTGYPRGLRGEQIPLAARIFALVDVWDALTNDRPYRKAWSREQAWQYIH
DQKGKHFDPQLVDLFLSIAAPDLTKVMERQVSGGKED
>Mature_357_residues
MQNQPYNVLVIDDDPNVRDILSSLLKRERCVVRTAASGQIGIAAARSELPDLILLDVMMPDLDGFSVCRMLREDPLTAEV
PIIMITALDDRASRLEGVKAGADEFLSKPVELSELRLRVRTMQRINRYRRLIEERERAAAQERLLTEQARQAAQAIAEAY
DQTLIGWSRALDLRDKETEGHSQRVAQLTLAVAQALNIPPEEQINMWRGAMLHDIGKIGVPDAILHKPGPLTEEEWEIMR
RHTTYAYELLYPIAYLRPALDIPYCHHEKWDGTGYPRGLRGEQIPLAARIFALVDVWDALTNDRPYRKAWSREQAWQYIH
DQKGKHFDPQLVDLFLSIAAPDLTKVMERQVSGGKED

Specific function: Positively regulates the synthesis of extracellular enzymes and extracellular polysaccharides. The cell-cell signaling system encoded by rpf genes is required for full virulence and is implicated in regulation of the formation and dispersal of biofilms [H

COG id: COG3437

COG function: function code KT; Response regulator containing a CheY-like receiver domain and an HD-GYP domain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1787229, Length=118, Percent_Identity=35.5932203389831, Blast_Score=78, Evalue=1e-15,
Organism=Escherichia coli, GI1788394, Length=162, Percent_Identity=29.6296296296296, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI1786784, Length=117, Percent_Identity=36.7521367521368, Blast_Score=76, Evalue=3e-15,
Organism=Escherichia coli, GI1790860, Length=123, Percent_Identity=32.520325203252, Blast_Score=76, Evalue=3e-15,
Organism=Escherichia coli, GI1789809, Length=119, Percent_Identity=36.1344537815126, Blast_Score=75, Evalue=5e-15,
Organism=Escherichia coli, GI1786911, Length=118, Percent_Identity=35.5932203389831, Blast_Score=75, Evalue=7e-15,
Organism=Escherichia coli, GI1786599, Length=117, Percent_Identity=28.2051282051282, Blast_Score=69, Evalue=6e-13,
Organism=Escherichia coli, GI87082012, Length=117, Percent_Identity=31.6239316239316, Blast_Score=65, Evalue=5e-12,
Organism=Escherichia coli, GI1788550, Length=131, Percent_Identity=26.7175572519084, Blast_Score=65, Evalue=7e-12,
Organism=Escherichia coli, GI1789149, Length=116, Percent_Identity=31.0344827586207, Blast_Score=62, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR003607
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00072 Response_reg [H]

EC number: NA

Molecular weight: Translated: 40761; Mature: 40761

Theoretical pI: Translated: 5.50; Mature: 5.50

Prosite motif: PS50110 RESPONSE_REGULATORY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQNQPYNVLVIDDDPNVRDILSSLLKRERCVVRTAASGQIGIAAARSELPDLILLDVMMP
CCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHEECCCCCEEEHHHHHCCCHHHHHHHHHC
DLDGFSVCRMLREDPLTAEVPIIMITALDDRASRLEGVKAGADEFLSKPVELSELRLRVR
CCCHHHHHHHHHHCCCCCCCCEEEEEECCHHHHHHHHHHCCHHHHHCCCCCHHHHHHHHH
TMQRINRYRRLIEERERAAAQERLLTEQARQAAQAIAEAYDQTLIGWSRALDLRDKETEG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
HSQRVAQLTLAVAQALNIPPEEQINMWRGAMLHDIGKIGVPDAILHKPGPLTEEEWEIMR
HHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCHHHHCCCCCCCHHHHHHHH
RHTTYAYELLYPIAYLRPALDIPYCHHEKWDGTGYPRGLRGEQIPLAARIFALVDVWDAL
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHH
TNDRPYRKAWSREQAWQYIHDQKGKHFDPQLVDLFLSIAAPDLTKVMERQVSGGKED
HCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MQNQPYNVLVIDDDPNVRDILSSLLKRERCVVRTAASGQIGIAAARSELPDLILLDVMMP
CCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHEECCCCCEEEHHHHHCCCHHHHHHHHHC
DLDGFSVCRMLREDPLTAEVPIIMITALDDRASRLEGVKAGADEFLSKPVELSELRLRVR
CCCHHHHHHHHHHCCCCCCCCEEEEEECCHHHHHHHHHHCCHHHHHCCCCCHHHHHHHHH
TMQRINRYRRLIEERERAAAQERLLTEQARQAAQAIAEAYDQTLIGWSRALDLRDKETEG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
HSQRVAQLTLAVAQALNIPPEEQINMWRGAMLHDIGKIGVPDAILHKPGPLTEEEWEIMR
HHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCHHHHCCCCCCCHHHHHHHH
RHTTYAYELLYPIAYLRPALDIPYCHHEKWDGTGYPRGLRGEQIPLAARIFALVDVWDAL
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHH
TNDRPYRKAWSREQAWQYIHDQKGKHFDPQLVDLFLSIAAPDLTKVMERQVSGGKED
HCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11123673 [H]