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The map label for this gene is hgiDIM [H]

Identifier: 222526394

GI number: 222526394

Start: 3918626

End: 3919594

Strand: Reverse

Name: hgiDIM [H]

Synonym: Chy400_3160

Alternate gene names: 222526394

Gene position: 3919594-3918626 (Counterclockwise)

Preceding gene: 222526395

Following gene: 222526393

Centisome position: 74.39

GC content: 52.12

Gene sequence:

>969_bases
ATGAATACCATTCGAGCTATCGATCTATTTTGTGGTATTGGTGGTAATTCCTGCGGTGCGCGAGCGGCAGGGATCGACAT
CGCTGCCGGTTTCGACAAATGGGCACTCGCCGGACAGGTATTTCAGGATAACTTCCCGGAAGCACGATTTTACAACGTTG
ATCTTGCCATCCTGTCACGAAGGCAGATTCACCACTTTCACGAAACAATTGGTCACGTTGATCTTATTCTTGCTTCACCG
GAGTGTACCAGCCACAGCGTGGCACGAGGCGCGAGTCCGAAAGACAAGGCCAGCCTTCGCCTTTCCTGGAATGTCTGGCG
GTTTGCAGAAGTGTTTCAACCACGATGGGTAGTGGTTGAAAATGTTCCCGCTTTTCGTTTATGGGAACACTACAGACATT
TTCTTGAGATCATGCAGCGTAGTGGTTATCGTGTTTTGGAGCAGATGCTGGTTGCCAGTGCATTCGGTGTGCCGCAAAGA
CGCCGTCGCCTGTATCTGCTCTTCGACCGTGATCGTGAACCACGCGCAGTTGTGCCCTGTCGTTATGAACCACTTCCAGC
ATCGTATGCGATAAACCTCAATGGCTCCTATCGTTATACACCACTGGTAACTGCAAACCGGGCTGCCCGCACGCTGGTAA
GAGCACAGAACGCTATCGATGTTCTGGGCAGGGATACACCCTTTTTGCTCGTGTATTACGGTTCGGACAAAGCTGGTGGC
TGGCAGTCACTTGACACACCACTGCGCACCATTACAACCCTGGACCGCTTTGCACTGGTGCGCCCGGACGGTCGTGGCGG
CCATGAGATGCGAATGCTTCAGCCGCCTGAATTGAAAAAAGCGATGGGGTTTCCGCCGGAATTTGTCATTAATCACGGTA
ACCGCAGAGAACAGATTAAGATGTTGGGAAACGCCGTGTGTCCGCCAGTGATGCAGGCTATCGTTCAGACGCTGATTGCT
GATACATGA

Upstream 100 bases:

>100_bases
CACAACGCGAGCGGGAGCGGGGAAGTAGCTGAAATGTGGCGAATAGCCCACTCTTCGTATACAATATGATCCTGCTGTTG
CAGGATCAGAGAAATCAGGC

Downstream 100 bases:

>100_bases
CCAACGATACGCTGCTTCCATTCAGCTCCGATGAGGTACAGGCCCTGCGTGTAGCGTTACACCAATGGGGGGCTGAGCAT
GTACGCTCATTTCCGTGGCG

Product: C-5 cytosine-specific DNA methylase

Products: NA

Alternate protein names: M.HgiDI; Cytosine-specific methyltransferase HgiDI [H]

Number of amino acids: Translated: 322; Mature: 322

Protein sequence:

>322_residues
MNTIRAIDLFCGIGGNSCGARAAGIDIAAGFDKWALAGQVFQDNFPEARFYNVDLAILSRRQIHHFHETIGHVDLILASP
ECTSHSVARGASPKDKASLRLSWNVWRFAEVFQPRWVVVENVPAFRLWEHYRHFLEIMQRSGYRVLEQMLVASAFGVPQR
RRRLYLLFDRDREPRAVVPCRYEPLPASYAINLNGSYRYTPLVTANRAARTLVRAQNAIDVLGRDTPFLLVYYGSDKAGG
WQSLDTPLRTITTLDRFALVRPDGRGGHEMRMLQPPELKKAMGFPPEFVINHGNRREQIKMLGNAVCPPVMQAIVQTLIA
DT

Sequences:

>Translated_322_residues
MNTIRAIDLFCGIGGNSCGARAAGIDIAAGFDKWALAGQVFQDNFPEARFYNVDLAILSRRQIHHFHETIGHVDLILASP
ECTSHSVARGASPKDKASLRLSWNVWRFAEVFQPRWVVVENVPAFRLWEHYRHFLEIMQRSGYRVLEQMLVASAFGVPQR
RRRLYLLFDRDREPRAVVPCRYEPLPASYAINLNGSYRYTPLVTANRAARTLVRAQNAIDVLGRDTPFLLVYYGSDKAGG
WQSLDTPLRTITTLDRFALVRPDGRGGHEMRMLQPPELKKAMGFPPEFVINHGNRREQIKMLGNAVCPPVMQAIVQTLIA
DT
>Mature_322_residues
MNTIRAIDLFCGIGGNSCGARAAGIDIAAGFDKWALAGQVFQDNFPEARFYNVDLAILSRRQIHHFHETIGHVDLILASP
ECTSHSVARGASPKDKASLRLSWNVWRFAEVFQPRWVVVENVPAFRLWEHYRHFLEIMQRSGYRVLEQMLVASAFGVPQR
RRRLYLLFDRDREPRAVVPCRYEPLPASYAINLNGSYRYTPLVTANRAARTLVRAQNAIDVLGRDTPFLLVYYGSDKAGG
WQSLDTPLRTITTLDRFALVRPDGRGGHEMRMLQPPELKKAMGFPPEFVINHGNRREQIKMLGNAVCPPVMQAIVQTLIA
DT

Specific function: This methylase recognizes the double-stranded sequence GRCGYC, causes specific methylation on C-? on both strands, and protects the DNA from cleavage by the HgiDI endonuclease [H]

COG id: COG0270

COG function: function code L; Site-specific DNA methylase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the C5-methyltransferase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018117
- InterPro:   IPR001525 [H]

Pfam domain/function: PF00145 DNA_methylase [H]

EC number: =2.1.1.37 [H]

Molecular weight: Translated: 36474; Mature: 36474

Theoretical pI: Translated: 9.84; Mature: 9.84

Prosite motif: PS00095 C5_MTASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNTIRAIDLFCGIGGNSCGARAAGIDIAAGFDKWALAGQVFQDNFPEARFYNVDLAILSR
CCCCEEEEEEECCCCCCCCCCCCCCEEECCCCHHHHHHHHHHCCCCCCEEEEEHHHHHHH
RQIHHFHETIGHVDLILASPECTSHSVARGASPKDKASLRLSWNVWRFAEVFQPRWVVVE
HHHHHHHHHHCCEEEEEECCCCCCCHHHCCCCCCCCCEEEEEECHHHHHHHCCCCEEEEE
NVPAFRLWEHYRHFLEIMQRSGYRVLEQMLVASAFGVPQRRRRLYLLFDRDREPRAVVPC
CCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCHHHCEEEEEEECCCCCCEECCC
RYEPLPASYAINLNGSYRYTPLVTANRAARTLVRAQNAIDVLGRDTPFLLVYYGSDKAGG
CCCCCCCEEEEEECCCEEEEEEEECCHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCC
WQSLDTPLRTITTLDRFALVRPDGRGGHEMRMLQPPELKKAMGFPPEFVINHGNRREQIK
CCCCCCHHHHHHHHHHHEEECCCCCCCCCEECCCCCHHHHHCCCCHHHEECCCCHHHHHH
MLGNAVCPPVMQAIVQTLIADT
HHHCCCCHHHHHHHHHHHHCCC
>Mature Secondary Structure
MNTIRAIDLFCGIGGNSCGARAAGIDIAAGFDKWALAGQVFQDNFPEARFYNVDLAILSR
CCCCEEEEEEECCCCCCCCCCCCCCEEECCCCHHHHHHHHHHCCCCCCEEEEEHHHHHHH
RQIHHFHETIGHVDLILASPECTSHSVARGASPKDKASLRLSWNVWRFAEVFQPRWVVVE
HHHHHHHHHHCCEEEEEECCCCCCCHHHCCCCCCCCCEEEEEECHHHHHHHCCCCEEEEE
NVPAFRLWEHYRHFLEIMQRSGYRVLEQMLVASAFGVPQRRRRLYLLFDRDREPRAVVPC
CCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCHHHCEEEEEEECCCCCCEECCC
RYEPLPASYAINLNGSYRYTPLVTANRAARTLVRAQNAIDVLGRDTPFLLVYYGSDKAGG
CCCCCCCEEEEEECCCEEEEEEEECCHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCC
WQSLDTPLRTITTLDRFALVRPDGRGGHEMRMLQPPELKKAMGFPPEFVINHGNRREQIK
CCCCCCHHHHHHHHHHHEEECCCCCCCCCEECCCCCHHHHHCCCCHHHEECCCCHHHHHH
MLGNAVCPPVMQAIVQTLIADT
HHHCCCCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2020544; 7607523 [H]