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The map label for this gene is mutY [C]

Identifier: 222526393

GI number: 222526393

Start: 3917946

End: 3918629

Strand: Reverse

Name: mutY [C]

Synonym: Chy400_3159

Alternate gene names: 222526393

Gene position: 3918629-3917946 (Counterclockwise)

Preceding gene: 222526394

Following gene: 222526392

Centisome position: 74.37

GC content: 56.58

Gene sequence:

>684_bases
ATGACCAACGATACGCTGCTTCCATTCAGCTCCGATGAGGTACAGGCCCTGCGTGTAGCGTTACACCAATGGGGGGCTGA
GCATGTACGCTCATTTCCGTGGCGGCAAACGACGGACCCATACCATATCTTACTGGCAGAGATTTTGCTACATCGGACAC
AGGTAAAGCAGGTTGTGCCGGTATATGAAGCCTTCCTGGCCCGCTATCCTGATCTCACATCGCTCTCACAGGCATCTCAG
GGTGACATCTATCAAATGCTCGGTTCACTGGGGTTAACCTGGCGATCCCGGTTGTTCTACGAGATGCTTCAGGCAATTGC
CACTCGCTACCACGGCAACGTTCCACGCCGCAAAGAGGATTTGCTCTCCCTTCCCGGTATCAGTGATTACATTGCCAGTG
CTGTACGTTGCTTTGCCTGGAACGAGCCAGAGGTTCTACTTGACACCAATACCGTGCGCATTACCGGACGACTGCTGGGC
TGGCCGGTCAGAGACTCATCCCGACGCAGCGCACGCTTTCGGCACGCACTTGCTTCGCTCCTCGACCATGAACAGCCACG
AATGTTCAACTACGCCCTGCTCGATCTGGCGCACCTGGTTTGCCTGACGCGCCGGCAACCGCTCTGCGCCCAATGCCCGC
TCAACACCTGGTGTACCTTCGCAACACAGGAGTCCACGGCGTGA

Upstream 100 bases:

>100_bases
TCATTAATCACGGTAACCGCAGAGAACAGATTAAGATGTTGGGAAACGCCGTGTGTCCGCCAGTGATGCAGGCTATCGTT
CAGACGCTGATTGCTGATAC

Downstream 100 bases:

>100_bases
CATCCGCTCACGATCTGACCGTCTGTCCATTTGTTGTCGGACCCAAAATTACCGATCTGCATCTGTTCGTGGGGCGAACA
GAATAGCTGGAGCAGACCAT

Product: helix-turn-helix domain-containing protein

Products: NA

Alternate protein names: Mismatch glycosylase; MIG; Thymine-DNA glycosylase [H]

Number of amino acids: Translated: 227; Mature: 226

Protein sequence:

>227_residues
MTNDTLLPFSSDEVQALRVALHQWGAEHVRSFPWRQTTDPYHILLAEILLHRTQVKQVVPVYEAFLARYPDLTSLSQASQ
GDIYQMLGSLGLTWRSRLFYEMLQAIATRYHGNVPRRKEDLLSLPGISDYIASAVRCFAWNEPEVLLDTNTVRITGRLLG
WPVRDSSRRSARFRHALASLLDHEQPRMFNYALLDLAHLVCLTRRQPLCAQCPLNTWCTFATQESTA

Sequences:

>Translated_227_residues
MTNDTLLPFSSDEVQALRVALHQWGAEHVRSFPWRQTTDPYHILLAEILLHRTQVKQVVPVYEAFLARYPDLTSLSQASQ
GDIYQMLGSLGLTWRSRLFYEMLQAIATRYHGNVPRRKEDLLSLPGISDYIASAVRCFAWNEPEVLLDTNTVRITGRLLG
WPVRDSSRRSARFRHALASLLDHEQPRMFNYALLDLAHLVCLTRRQPLCAQCPLNTWCTFATQESTA
>Mature_226_residues
TNDTLLPFSSDEVQALRVALHQWGAEHVRSFPWRQTTDPYHILLAEILLHRTQVKQVVPVYEAFLARYPDLTSLSQASQG
DIYQMLGSLGLTWRSRLFYEMLQAIATRYHGNVPRRKEDLLSLPGISDYIASAVRCFAWNEPEVLLDTNTVRITGRLLGW
PVRDSSRRSARFRHALASLLDHEQPRMFNYALLDLAHLVCLTRRQPLCAQCPLNTWCTFATQESTA

Specific function: Could act in DNA repair. Restriction methylase M.mthtI, which is encoded by this plasmid, generates 5-methylcytosine which is, especially under thermophilic conditions, subject to deamination resulting in G-T mismatches. This protein could correct these m

COG id: COG1194

COG function: function code L; A/G-specific DNA glycosylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Nth/MutY family [H]

Homologues:

Organism=Homo sapiens, GI115298648, Length=216, Percent_Identity=30.5555555555556, Blast_Score=107, Evalue=9e-24,
Organism=Homo sapiens, GI115298654, Length=216, Percent_Identity=30.5555555555556, Blast_Score=107, Evalue=1e-23,
Organism=Homo sapiens, GI115298652, Length=216, Percent_Identity=30.5555555555556, Blast_Score=107, Evalue=1e-23,
Organism=Homo sapiens, GI115298650, Length=216, Percent_Identity=30.5555555555556, Blast_Score=107, Evalue=1e-23,
Organism=Homo sapiens, GI190358497, Length=216, Percent_Identity=30.5555555555556, Blast_Score=107, Evalue=1e-23,
Organism=Homo sapiens, GI6912520, Length=216, Percent_Identity=30.5555555555556, Blast_Score=107, Evalue=1e-23,
Organism=Escherichia coli, GI1789331, Length=205, Percent_Identity=31.219512195122, Blast_Score=115, Evalue=2e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011257
- InterPro:   IPR004036
- InterPro:   IPR004035
- InterPro:   IPR003651
- InterPro:   IPR003265
- InterPro:   IPR000445
- InterPro:   IPR023170 [H]

Pfam domain/function: PF00633 HHH; PF00730 HhH-GPD [H]

EC number: 3.2.2.-

Molecular weight: Translated: 26069; Mature: 25938

Theoretical pI: Translated: 8.23; Mature: 8.23

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTNDTLLPFSSDEVQALRVALHQWGAEHVRSFPWRQTTDPYHILLAEILLHRTQVKQVVP
CCCCCCCCCCCHHHHHHHHHHHHHCHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
VYEAFLARYPDLTSLSQASQGDIYQMLGSLGLTWRSRLFYEMLQAIATRYHGNVPRRKED
HHHHHHHHCCCHHHHHHCCCCHHHHHHHHHCHHHHHHHHHHHHHHHHHHHCCCCCCCHHH
LLSLPGISDYIASAVRCFAWNEPEVLLDTNTVRITGRLLGWPVRDSSRRSARFRHALASL
HHCCCCHHHHHHHHHHHHCCCCCCEEEECCEEEEEHHHHCCCCCCCHHHHHHHHHHHHHH
LDHEQPRMFNYALLDLAHLVCLTRRQPLCAQCPLNTWCTFATQESTA
HCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHEEECCCCCCC
>Mature Secondary Structure 
TNDTLLPFSSDEVQALRVALHQWGAEHVRSFPWRQTTDPYHILLAEILLHRTQVKQVVP
CCCCCCCCCCHHHHHHHHHHHHHCHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
VYEAFLARYPDLTSLSQASQGDIYQMLGSLGLTWRSRLFYEMLQAIATRYHGNVPRRKED
HHHHHHHHCCCHHHHHHCCCCHHHHHHHHHCHHHHHHHHHHHHHHHHHHHCCCCCCCHHH
LLSLPGISDYIASAVRCFAWNEPEVLLDTNTVRITGRLLGWPVRDSSRRSARFRHALASL
HHCCCCHHHHHHHHHHHHCCCCCCEEEECCEEEEEHHHHCCCCCCCHHHHHHHHHHHHHH
LDHEQPRMFNYALLDLAHLVCLTRRQPLCAQCPLNTWCTFATQESTA
HCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: 4Fe-4S Cluster [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Glycosylases; Hydrolysing N-glycosyl compounds [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1336177; 11786018 [H]