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The map label for this gene is hydA [H]

Identifier: 222526234

GI number: 222526234

Start: 3699939

End: 3700910

Strand: Reverse

Name: hydA [H]

Synonym: Chy400_2996

Alternate gene names: 222526234

Gene position: 3700910-3699939 (Counterclockwise)

Preceding gene: 222526235

Following gene: 222526233

Centisome position: 70.24

GC content: 59.77

Gene sequence:

>972_bases
ATGGCAACATTGCTCTGGTTACAGGGTGGCGCCTGTAGCGGCAACACGATGTCATTCCTCAATGCCGAGGAACCAAGTGC
GTGTGATCTGGTCACCGATTTTGGAATTGAGATTCTGTGGCACCCGTCGCTCGGCATGGAGCTGGGTGAGAACGCCAAAC
GTATCTTTCACGACTGTGCCAGTGGCGCCCGCCCGCTTGACATCTTTGTCTTCGAGGGTACGGTGATCCAGGCCCCGAAC
GGCACTGGCCGCTACAACATGTTTGCCGACCGCCCAATGCAAGAGTGGGTGCGCGAACTGGCTGCGCAGGCCAGCATTGT
GGTCGCCATCGGTGATTGCGCCTGCTGGGGCGGCATCCCGGCCATGGCCCCTAATCCCAGTCAGTCCACCGGTCTTCAGT
TTCACAAGCGCGAGCTGGGCGGTTTCCTCGGCCCCAACTGGCGCTCGAAGGCCGGTCTGCCGGTGATTAACATTCCCGGC
TGTCCGGCTCACCCCGACTGGGTGACCCAGATTCTGGTCGCGGTTGCCAGTGGTCGCGCCGCCGACATCGCCCTCGACGA
TCTGCAACGTCCGCAGACCTTCTTTACCAGCTTCACCCAAACCGGCTGTACACGGGTACAATTTTTCGAGTACAAACAAT
CAACGCTCGAATTTGGGCAGGGCACGCGCACCGGCTGTCTCTTCTACGAGTTTGGTTGTCGTGGCCCGATGACTCGCTCA
CCCTGCAATCGCATTCTCTGGAACCGCCAGTCATCGAAGACCCGCGCCGGTATGCCCTGTACGGGTTGTACCGAACCGGA
GTTTCCCTTCTTCGATCTCGCGCCCGGCACCGTCTTCAAAACCCAGAAGATCAGCGGGGCGATCCCGAAAGAGGTGCCAA
CCGGAACCGATCCGATCAGCTATATGGCACTGGCCGCAGCCGCTCGTGTTGCTGCGCCCAAGTGGGCCAAAGAAGATATG
TTCGTTGTGTAG

Upstream 100 bases:

>100_bases
ACCAGATGCGAACACGATCATCAAATTCAAAAACACCATCGGCGTAGCGCTGTTTTTGATAGGGAAGTCTAGCATCTTGA
GAGGTGAAGGAGGCGGTTCA

Downstream 100 bases:

>100_bases
TAAGGGGAGAAGCTGGCAATGGTTAATATCATCGAGAATCCGGCAACACTTACCGGTCGTGACTTGCGCATCAGCCCACT
TGGCCGTGTCGAAGGTGACC

Product: NADH ubiquinone oxidoreductase 20 kDa subunit

Products: NA

Alternate protein names: NiFe hydrogenlyase small chain [H]

Number of amino acids: Translated: 323; Mature: 322

Protein sequence:

>323_residues
MATLLWLQGGACSGNTMSFLNAEEPSACDLVTDFGIEILWHPSLGMELGENAKRIFHDCASGARPLDIFVFEGTVIQAPN
GTGRYNMFADRPMQEWVRELAAQASIVVAIGDCACWGGIPAMAPNPSQSTGLQFHKRELGGFLGPNWRSKAGLPVINIPG
CPAHPDWVTQILVAVASGRAADIALDDLQRPQTFFTSFTQTGCTRVQFFEYKQSTLEFGQGTRTGCLFYEFGCRGPMTRS
PCNRILWNRQSSKTRAGMPCTGCTEPEFPFFDLAPGTVFKTQKISGAIPKEVPTGTDPISYMALAAAARVAAPKWAKEDM
FVV

Sequences:

>Translated_323_residues
MATLLWLQGGACSGNTMSFLNAEEPSACDLVTDFGIEILWHPSLGMELGENAKRIFHDCASGARPLDIFVFEGTVIQAPN
GTGRYNMFADRPMQEWVRELAAQASIVVAIGDCACWGGIPAMAPNPSQSTGLQFHKRELGGFLGPNWRSKAGLPVINIPG
CPAHPDWVTQILVAVASGRAADIALDDLQRPQTFFTSFTQTGCTRVQFFEYKQSTLEFGQGTRTGCLFYEFGCRGPMTRS
PCNRILWNRQSSKTRAGMPCTGCTEPEFPFFDLAPGTVFKTQKISGAIPKEVPTGTDPISYMALAAAARVAAPKWAKEDM
FVV
>Mature_322_residues
ATLLWLQGGACSGNTMSFLNAEEPSACDLVTDFGIEILWHPSLGMELGENAKRIFHDCASGARPLDIFVFEGTVIQAPNG
TGRYNMFADRPMQEWVRELAAQASIVVAIGDCACWGGIPAMAPNPSQSTGLQFHKRELGGFLGPNWRSKAGLPVINIPGC
PAHPDWVTQILVAVASGRAADIALDDLQRPQTFFTSFTQTGCTRVQFFEYKQSTLEFGQGTRTGCLFYEFGCRGPMTRSP
CNRILWNRQSSKTRAGMPCTGCTEPEFPFFDLAPGTVFKTQKISGAIPKEVPTGTDPISYMALAAAARVAAPKWAKEDMF
VV

Specific function: This Is One Of Three E.Coli Hydrogenases Synthesized In Response To Different Physiological Conditions. Hyd2 Is Involved In Hydrogen Uptake. [C]

COG id: COG1740

COG function: function code C; Ni,Fe-hydrogenase I small subunit

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the [NiFe]/[NiFeSe] hydrogenase small subunit family [H]

Homologues:

Organism=Escherichia coli, GI1789371, Length=272, Percent_Identity=28.3088235294118, Blast_Score=103, Evalue=2e-23,
Organism=Escherichia coli, GI1787206, Length=271, Percent_Identity=29.520295202952, Blast_Score=97, Evalue=1e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006137
- InterPro:   IPR001821 [H]

Pfam domain/function: PF01058 Oxidored_q6 [H]

EC number: =1.12.2.1 [H]

Molecular weight: Translated: 35119; Mature: 34988

Theoretical pI: Translated: 6.49; Mature: 6.49

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.7 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
6.8 %Cys+Met (Translated Protein)
3.7 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
6.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATLLWLQGGACSGNTMSFLNAEEPSACDLVTDFGIEILWHPSLGMELGENAKRIFHDCA
CCEEEEEECCCCCCCCEEEECCCCCCHHHHHHCCCEEEEECCCCCCHHCHHHHHHHHHHH
SGARPLDIFVFEGTVIQAPNGTGRYNMFADRPMQEWVRELAAQASIVVAIGDCACWGGIP
CCCCCEEEEEECCEEEECCCCCCCCCEEECCHHHHHHHHHHHCCEEEEEEECCHHHCCCC
AMAPNPSQSTGLQFHKRELGGFLGPNWRSKAGLPVINIPGCPAHPDWVTQILVAVASGRA
CCCCCCCCCCCCCHHHHHHCCCCCCCCCCCCCCCEEECCCCCCCHHHHHHHHHHHHCCCC
ADIALDDLQRPQTFFTSFTQTGCTRVQFFEYKQSTLEFGQGTRTGCLFYEFGCRGPMTRS
CEEEHHHHCCHHHHHHHHHHCCCCEEEEEHHHHHHHHHCCCCCCEEEEEEECCCCCCCCC
PCNRILWNRQSSKTRAGMPCTGCTEPEFPFFDLAPGTVFKTQKISGAIPKEVPTGTDPIS
CHHHHHCCCCCCCCCCCCCCCCCCCCCCCEEECCCCCEEEECCCCCCCCCCCCCCCCHHH
YMALAAAARVAAPKWAKEDMFVV
HHHHHHHHHHCCCCCCCCCCCCC
>Mature Secondary Structure 
ATLLWLQGGACSGNTMSFLNAEEPSACDLVTDFGIEILWHPSLGMELGENAKRIFHDCA
CEEEEEECCCCCCCCEEEECCCCCCHHHHHHCCCEEEEECCCCCCHHCHHHHHHHHHHH
SGARPLDIFVFEGTVIQAPNGTGRYNMFADRPMQEWVRELAAQASIVVAIGDCACWGGIP
CCCCCEEEEEECCEEEECCCCCCCCCEEECCHHHHHHHHHHHCCEEEEEEECCHHHCCCC
AMAPNPSQSTGLQFHKRELGGFLGPNWRSKAGLPVINIPGCPAHPDWVTQILVAVASGRA
CCCCCCCCCCCCCHHHHHHCCCCCCCCCCCCCCCEEECCCCCCCHHHHHHHHHHHHCCCC
ADIALDDLQRPQTFFTSFTQTGCTRVQFFEYKQSTLEFGQGTRTGCLFYEFGCRGPMTRS
CEEEHHHHCCHHHHHHHHHHCCCCEEEEEHHHHHHHHHCCCCCCEEEEEEECCCCCCCCC
PCNRILWNRQSSKTRAGMPCTGCTEPEFPFFDLAPGTVFKTQKISGAIPKEVPTGTDPIS
CHHHHHCCCCCCCCCCCCCCCCCCCCCCCEEECCCCCEEEECCCCCCCCCCCCCCCCHHH
YMALAAAARVAAPKWAKEDMFVV
HHHHHHHHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3322743; 2651421; 3322275; 3134950; 7854413 [H]