Click here to switch to the map view.

The map label for this gene is hybC [C]

Identifier: 222526233

GI number: 222526233

Start: 3698283

End: 3699920

Strand: Reverse

Name: hybC [C]

Synonym: Chy400_2995

Alternate gene names: 222526233

Gene position: 3699920-3698283 (Counterclockwise)

Preceding gene: 222526234

Following gene: 222526232

Centisome position: 70.22

GC content: 59.28

Gene sequence:

>1638_bases
ATGGTTAATATCATCGAGAATCCGGCAACACTTACCGGTCGTGACTTGCGCATCAGCCCACTTGGCCGTGTCGAAGGTGA
CCTCGATCTGCGGGTGACCATCCGCGACGGCGTTGTCACCAGTGCGTGGACCGAAGCCTCAATGTTTCGCGGCTTCGAGA
TTATTCTGAAGGGCAAAGACCCGCAGGCCGGGTTGATTGTCACCCCTCGCATCTGTGGTATTTGCGGCGGTAGCCATCTG
ACCAAAGCCGTGTATGCGCTGGACACCGCATGGCAGACCGAGCTGCCACCCAATGCCACCCTGATCCGCAACATCGCCCA
GGCGTGCGAGACCTTGCAGAGCATTCCGCGCTGGTTCTACGCTCTCTTCGCCATCGATTTGACGAACAAGAAGTACGCCC
ATCTGCCCGAATACGATGAAGCGGTGCGCCGCTTCGCACCCTTCGTCGGCACCAGTTACGAGCACGGGGTGACCCTCTCG
AATAAGCCGGTAGAGATTTACGCTATCTTCGGCGGCCAGTGGCCTCACTCCAGTTTCATGATCCCCGGCGGTGTCATGTG
TGCGCCAACGCTGGCCGATGTGACCCGCGCCATCGCCATTCTTGAGTACTGGAAAGACGAATGGCTGGAGAAGAAGTGGC
TCGGCTGTTCGGTTGATCGCTGGCTGCAAAACAAGAGCTGGGCCGATGTGATGGAATGGATGCACGAGAACGAGCGCCAT
TACAATTCCGACTGCGGCTTCTTCCTGCGCTTCGCGATGGCCGCCGGTCTCGACAAATATGGCGCCGGGTGGAATAACTA
TCTTGCCACCGGTACCTACTTCCACCCCGAACTGTACGCTCGCCCCACCATCGAAGGGCGCAACGCTGCCCTGATTGCCC
GTTCCGGCGTCTACGTGAACGGTCAGTTCTACGACTTCGATCAGGCTCAGGTCCGCGAAGATGTCAGCCACTCCTTCTAC
GAGGGGAACCATGTCTTGCACCCATTTGAGGGGCGTACCGAACCAATTGACCCGGAAGTTGGCCGGCGACAGGGCAAGTA
CACCTGGGCCAAAGCTCCCCGCTATCTCATCCCCGGCGTAGGGAGCCATCCGCTGGAGGCCGGCCCGCTGGCGCGCCAGG
TGATTGCCGGTCGTCCAGGCGCTGCCGACTGGCAGGACTACGATCCGCTCTTCCTCGATGCCGTAACCACCGTCGGGCCG
AGTGTGTTGGTGCGGGTGATGGCTCGGATGCACGAAGCACCGAAATACTACAAGCTGGTGCGCAAATGGCTCGATCAGAT
CAACCTGCACGAGAAGTTCTACATCAAGCCAAAAGAACTACCGGAAGGGCGTGGTTTTGGCTCAACTGAAGCTGCTCGTG
GCAGCCTCTCCGACTGGATCGTTTTGAAGGACGGTAAGATCGAGAATTATCAGGTGATTACACCGACGGCGTGGAACATC
GGCCCGCGTGATAGCCGCGAGGTCAATGGGCCGATGGAACAGGCGTTCCTCGGCGCTCCCATTGCCGACCCCAACGATCC
GGTCGAACTTGGTCATGTGGCGCGGAGCTACGACTCATGCCTGGTCTGTACGGTACACGCCTACGACGAGAAGACCGGCA
AGGAGTTGGCACGGTTCCGCATTGGTGAAGGCGCGTAG

Upstream 100 bases:

>100_bases
TCCGATCAGCTATATGGCACTGGCCGCAGCCGCTCGTGTTGCTGCGCCCAAGTGGGCCAAAGAAGATATGTTCGTTGTGT
AGTAAGGGGAGAAGCTGGCA

Downstream 100 bases:

>100_bases
CAGGGGCAAGCGCGCAGGAACGTCTGCACCGGTACTGGTCACCATGCCGGTGCAGACGAACTCGCATTGTTCGTGCTGGC
AATATCTGAACAGGTACGAT

Product: nickel-dependent hydrogenase large subunit

Products: NA

Alternate protein names: NiFeSe hydrogenlyase large chain [H]

Number of amino acids: Translated: 545; Mature: 545

Protein sequence:

>545_residues
MVNIIENPATLTGRDLRISPLGRVEGDLDLRVTIRDGVVTSAWTEASMFRGFEIILKGKDPQAGLIVTPRICGICGGSHL
TKAVYALDTAWQTELPPNATLIRNIAQACETLQSIPRWFYALFAIDLTNKKYAHLPEYDEAVRRFAPFVGTSYEHGVTLS
NKPVEIYAIFGGQWPHSSFMIPGGVMCAPTLADVTRAIAILEYWKDEWLEKKWLGCSVDRWLQNKSWADVMEWMHENERH
YNSDCGFFLRFAMAAGLDKYGAGWNNYLATGTYFHPELYARPTIEGRNAALIARSGVYVNGQFYDFDQAQVREDVSHSFY
EGNHVLHPFEGRTEPIDPEVGRRQGKYTWAKAPRYLIPGVGSHPLEAGPLARQVIAGRPGAADWQDYDPLFLDAVTTVGP
SVLVRVMARMHEAPKYYKLVRKWLDQINLHEKFYIKPKELPEGRGFGSTEAARGSLSDWIVLKDGKIENYQVITPTAWNI
GPRDSREVNGPMEQAFLGAPIADPNDPVELGHVARSYDSCLVCTVHAYDEKTGKELARFRIGEGA

Sequences:

>Translated_545_residues
MVNIIENPATLTGRDLRISPLGRVEGDLDLRVTIRDGVVTSAWTEASMFRGFEIILKGKDPQAGLIVTPRICGICGGSHL
TKAVYALDTAWQTELPPNATLIRNIAQACETLQSIPRWFYALFAIDLTNKKYAHLPEYDEAVRRFAPFVGTSYEHGVTLS
NKPVEIYAIFGGQWPHSSFMIPGGVMCAPTLADVTRAIAILEYWKDEWLEKKWLGCSVDRWLQNKSWADVMEWMHENERH
YNSDCGFFLRFAMAAGLDKYGAGWNNYLATGTYFHPELYARPTIEGRNAALIARSGVYVNGQFYDFDQAQVREDVSHSFY
EGNHVLHPFEGRTEPIDPEVGRRQGKYTWAKAPRYLIPGVGSHPLEAGPLARQVIAGRPGAADWQDYDPLFLDAVTTVGP
SVLVRVMARMHEAPKYYKLVRKWLDQINLHEKFYIKPKELPEGRGFGSTEAARGSLSDWIVLKDGKIENYQVITPTAWNI
GPRDSREVNGPMEQAFLGAPIADPNDPVELGHVARSYDSCLVCTVHAYDEKTGKELARFRIGEGA
>Mature_545_residues
MVNIIENPATLTGRDLRISPLGRVEGDLDLRVTIRDGVVTSAWTEASMFRGFEIILKGKDPQAGLIVTPRICGICGGSHL
TKAVYALDTAWQTELPPNATLIRNIAQACETLQSIPRWFYALFAIDLTNKKYAHLPEYDEAVRRFAPFVGTSYEHGVTLS
NKPVEIYAIFGGQWPHSSFMIPGGVMCAPTLADVTRAIAILEYWKDEWLEKKWLGCSVDRWLQNKSWADVMEWMHENERH
YNSDCGFFLRFAMAAGLDKYGAGWNNYLATGTYFHPELYARPTIEGRNAALIARSGVYVNGQFYDFDQAQVREDVSHSFY
EGNHVLHPFEGRTEPIDPEVGRRQGKYTWAKAPRYLIPGVGSHPLEAGPLARQVIAGRPGAADWQDYDPLFLDAVTTVGP
SVLVRVMARMHEAPKYYKLVRKWLDQINLHEKFYIKPKELPEGRGFGSTEAARGSLSDWIVLKDGKIENYQVITPTAWNI
GPRDSREVNGPMEQAFLGAPIADPNDPVELGHVARSYDSCLVCTVHAYDEKTGKELARFRIGEGA

Specific function: This Is One Of Three E.Coli Hydrogenases Synthesized In Response To Different Physiological Conditions. Hyd2 Is Involved In Hydrogen Uptake. [C]

COG id: COG0374

COG function: function code C; Ni,Fe-hydrogenase I large subunit

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the [NiFe]/[NiFeSe] hydrogenase large subunit family [H]

Homologues:

Organism=Escherichia coli, GI1789368, Length=587, Percent_Identity=27.5979557069847, Blast_Score=189, Evalue=3e-49,
Organism=Escherichia coli, GI1787207, Length=616, Percent_Identity=27.2727272727273, Blast_Score=187, Evalue=1e-48,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001501
- InterPro:   IPR018194 [H]

Pfam domain/function: PF00374 NiFeSe_Hases [H]

EC number: =1.12.99.6 [H]

Molecular weight: Translated: 61189; Mature: 61189

Theoretical pI: Translated: 6.46; Mature: 6.46

Prosite motif: PS00507 NI_HGENASE_L_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVNIIENPATLTGRDLRISPLGRVEGDLDLRVTIRDGVVTSAWTEASMFRGFEIILKGKD
CCEEECCCCCCCCCCEEECCCCCCCCCEEEEEEEECCCEEHHHHHHHHHCCEEEEEECCC
PQAGLIVTPRICGICGGSHLTKAVYALDTAWQTELPPNATLIRNIAQACETLQSIPRWFY
CCCCEEECCHHHHHCCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
ALFAIDLTNKKYAHLPEYDEAVRRFAPFVGTSYEHGVTLSNKPVEIYAIFGGQWPHSSFM
HHHHEECCCCCCCCCCCHHHHHHHHHHHHCCCCCCCEEECCCCEEEEEEECCCCCCCCEE
IPGGVMCAPTLADVTRAIAILEYWKDEWLEKKWLGCSVDRWLQNKSWADVMEWMHENERH
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCHHHHHHHHHCCCCC
YNSDCGFFLRFAMAAGLDKYGAGWNNYLATGTYFHPELYARPTIEGRNAALIARSGVYVN
CCCCHHHHHHHHHHHCCHHHCCCCCCCCCCCEEECCHHEECCCCCCCCEEEEEECCEEEC
GQFYDFDQAQVREDVSHSFYEGNHVLHPFEGRTEPIDPEVGRRQGKYTWAKAPRYLIPGV
CEEECCCHHHHHHHHHHHHHCCCCEECCCCCCCCCCCCHHHCCCCCEEHHCCCCEECCCC
GSHPLEAGPLARQVIAGRPGAADWQDYDPLFLDAVTTVGPSVLVRVMARMHEAPKYYKLV
CCCCCCCCHHHHHHHCCCCCCCCCCCCCCHHHHHHHHCCHHHHHHHHHHHHCCHHHHHHH
RKWLDQINLHEKFYIKPKELPEGRGFGSTEAARGSLSDWIVLKDGKIENYQVITPTAWNI
HHHHHHCCCCCEEEECCCCCCCCCCCCCCHHCCCCCCCEEEEECCCCCCEEEECCCCCCC
GPRDSREVNGPMEQAFLGAPIADPNDPVELGHVARSYDSCLVCTVHAYDEKTGKELARFR
CCCCCCCCCCCHHHHHHCCCCCCCCCCHHHHHHHHCCCCEEEEEEECCCCHHHHHHHHHH
IGEGA
CCCCC
>Mature Secondary Structure
MVNIIENPATLTGRDLRISPLGRVEGDLDLRVTIRDGVVTSAWTEASMFRGFEIILKGKD
CCEEECCCCCCCCCCEEECCCCCCCCCEEEEEEEECCCEEHHHHHHHHHCCEEEEEECCC
PQAGLIVTPRICGICGGSHLTKAVYALDTAWQTELPPNATLIRNIAQACETLQSIPRWFY
CCCCEEECCHHHHHCCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
ALFAIDLTNKKYAHLPEYDEAVRRFAPFVGTSYEHGVTLSNKPVEIYAIFGGQWPHSSFM
HHHHEECCCCCCCCCCCHHHHHHHHHHHHCCCCCCCEEECCCCEEEEEEECCCCCCCCEE
IPGGVMCAPTLADVTRAIAILEYWKDEWLEKKWLGCSVDRWLQNKSWADVMEWMHENERH
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCHHHHHHHHHCCCCC
YNSDCGFFLRFAMAAGLDKYGAGWNNYLATGTYFHPELYARPTIEGRNAALIARSGVYVN
CCCCHHHHHHHHHHHCCHHHCCCCCCCCCCCEEECCHHEECCCCCCCCEEEEEECCEEEC
GQFYDFDQAQVREDVSHSFYEGNHVLHPFEGRTEPIDPEVGRRQGKYTWAKAPRYLIPGV
CEEECCCHHHHHHHHHHHHHCCCCEECCCCCCCCCCCCHHHCCCCCEEHHCCCCEECCCC
GSHPLEAGPLARQVIAGRPGAADWQDYDPLFLDAVTTVGPSVLVRVMARMHEAPKYYKLV
CCCCCCCCHHHHHHHCCCCCCCCCCCCCCHHHHHHHHCCHHHHHHHHHHHHCCHHHHHHH
RKWLDQINLHEKFYIKPKELPEGRGFGSTEAARGSLSDWIVLKDGKIENYQVITPTAWNI
HHHHHHCCCCCEEEECCCCCCCCCCCCCCHHCCCCCCCEEEEECCCCCCEEEECCCCCCC
GPRDSREVNGPMEQAFLGAPIADPNDPVELGHVARSYDSCLVCTVHAYDEKTGKELARFR
CCCCCCCCCCCHHHHHHCCCCCCCCCCHHHHHHHHCCCCEEEEEEECCCCHHHHHHHHHH
IGEGA
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3316183; 10378275 [H]