Click here to switch to the map view.

The map label for this gene is cbiF [H]

Identifier: 222526017

GI number: 222526017

Start: 3418529

End: 3419353

Strand: Reverse

Name: cbiF [H]

Synonym: Chy400_2773

Alternate gene names: 222526017

Gene position: 3419353-3418529 (Counterclockwise)

Preceding gene: 222526018

Following gene: 222526016

Centisome position: 64.9

GC content: 62.06

Gene sequence:

>825_bases
ATGACCTACCCTGCTATTCCAGGAACTGTCTACTTCGTGGGTGCCGGCCCCGGTGCACCCGATTTGATAACAGTGCGTGG
CCGTGATCTGCTGGCCCAGGCCGATCTCATTCTCTACGCCGATAGTCTGGTTGACGCGGCCTTGCCGGCAGCCTACGCTC
GCGCCGATGCCCGCATCCTCGGTTCGGTTGAGATGCACCTCGAACAAATTGTGCGGCTGATGTGCGAGGCAGCGCGAGCC
GGCCAGGTCGTTGTGCGGCTGCATAGTGGCGATCCGGCACTCTACGGCGCCATCCACGAGCAGATGGCTGCTCTCGACGA
AGCCGGTGTGCCTTACGAGATTGTACCGGGTGTGACTGCTGCATTTGCGCTCGCCGCCCGCCTGGGGGTTGAGCTGACGG
TTCCTGAACTGGTGCAGACAATCATCCTGACCCGGCCCGCCGGACGCACACCGCTCCCCGAACACGAACAACTGAGCCGA
ATGGCGGCCCATGGCGCATCACTGGCGATCTATCTCGGCATTACCCGGATGCAACAGGTCGTGCGTGATCTGCTGACCGG
TGGCGTCTACACGCCGGACACACCGGTCGTTGTTGCCTACCGCATTACCTGGCCCGACGAGGTGATCATTCACGGCACAT
TGGGCGACATCGCCGAAAAGGTGAAAGCTGCCGGCTTTACCCGGCAGGCATTGATCCTGGTTAGCCCTGCGCTTGATCCG
GCCAACAAGCGTACCGACCGGCCAACCAGCCGGCTCTACGATCCAACCTACAGTCATCGGTTGCGCCGTCGCCGTGAACC
GGCTGATGAGACGGCAGAGGCATAA

Upstream 100 bases:

>100_bases
TTGGCTTAGCCGGTGAGTTTATCACTCGTGATCTGCGCACACTCGACCTGAACCATCGTCCGTATCTGTCACTCGTAATT
GTTCGTCGCGGAGAGCTACT

Downstream 100 bases:

>100_bases
CAGCCGGGAAGAGGTTCGTATGACAACAACAGCCATCTTTGCCCTCACCCGTAACGGCGTTGAACTGGCCACTCGCCTGG
CCGCAACGTTACCGGCAACT

Product: precorrin-4 C11-methyltransferase

Products: S-adenosyl-L-homocysteine; Precorrin 5

Alternate protein names: Cobalt-precorrin-3 methylase [H]

Number of amino acids: Translated: 274; Mature: 273

Protein sequence:

>274_residues
MTYPAIPGTVYFVGAGPGAPDLITVRGRDLLAQADLILYADSLVDAALPAAYARADARILGSVEMHLEQIVRLMCEAARA
GQVVVRLHSGDPALYGAIHEQMAALDEAGVPYEIVPGVTAAFALAARLGVELTVPELVQTIILTRPAGRTPLPEHEQLSR
MAAHGASLAIYLGITRMQQVVRDLLTGGVYTPDTPVVVAYRITWPDEVIIHGTLGDIAEKVKAAGFTRQALILVSPALDP
ANKRTDRPTSRLYDPTYSHRLRRRREPADETAEA

Sequences:

>Translated_274_residues
MTYPAIPGTVYFVGAGPGAPDLITVRGRDLLAQADLILYADSLVDAALPAAYARADARILGSVEMHLEQIVRLMCEAARA
GQVVVRLHSGDPALYGAIHEQMAALDEAGVPYEIVPGVTAAFALAARLGVELTVPELVQTIILTRPAGRTPLPEHEQLSR
MAAHGASLAIYLGITRMQQVVRDLLTGGVYTPDTPVVVAYRITWPDEVIIHGTLGDIAEKVKAAGFTRQALILVSPALDP
ANKRTDRPTSRLYDPTYSHRLRRRREPADETAEA
>Mature_273_residues
TYPAIPGTVYFVGAGPGAPDLITVRGRDLLAQADLILYADSLVDAALPAAYARADARILGSVEMHLEQIVRLMCEAARAG
QVVVRLHSGDPALYGAIHEQMAALDEAGVPYEIVPGVTAAFALAARLGVELTVPELVQTIILTRPAGRTPLPEHEQLSRM
AAHGASLAIYLGITRMQQVVRDLLTGGVYTPDTPVVVAYRITWPDEVIIHGTLGDIAEKVKAAGFTRQALILVSPALDPA
NKRTDRPTSRLYDPTYSHRLRRRREPADETAEA

Specific function: Catalyzes the methylation of C-11 in cobalt-precorrin-4 to form cobalt-precorrin-5 [H]

COG id: COG2875

COG function: function code H; Precorrin-4 methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the precorrin methyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI1789768, Length=223, Percent_Identity=29.5964125560538, Blast_Score=95, Evalue=4e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000878
- InterPro:   IPR014777
- InterPro:   IPR014776
- InterPro:   IPR006362
- InterPro:   IPR003043 [H]

Pfam domain/function: PF00590 TP_methylase [H]

EC number: 2.1.1.133

Molecular weight: Translated: 29513; Mature: 29382

Theoretical pI: Translated: 6.16; Mature: 6.16

Prosite motif: PS00839 SUMT_1 ; PS00840 SUMT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTYPAIPGTVYFVGAGPGAPDLITVRGRDLLAQADLILYADSLVDAALPAAYARADARIL
CCCCCCCCEEEEEECCCCCCCEEEECCCHHHHHHHHHEEHHHHHHHHHHHHHHHHHHHHH
GSVEMHLEQIVRLMCEAARAGQVVVRLHSGDPALYGAIHEQMAALDEAGVPYEIVPGVTA
HHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCHHHHHHHHHHHHHHHCCCCEEECCCHHH
AFALAARLGVELTVPELVQTIILTRPAGRTPLPEHEQLSRMAAHGASLAIYLGITRMQQV
HHHHHHHHCCEEEHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHCCCCEEEEHHHHHHHHH
VRDLLTGGVYTPDTPVVVAYRITWPDEVIIHGTLGDIAEKVKAAGFTRQALILVSPALDP
HHHHHHCCCCCCCCCEEEEEEEECCCCEEEECCHHHHHHHHHHCCCCCEEEEEEECCCCC
ANKRTDRPTSRLYDPTYSHRLRRRREPADETAEA
CCCCCCCCHHHCCCCHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure 
TYPAIPGTVYFVGAGPGAPDLITVRGRDLLAQADLILYADSLVDAALPAAYARADARIL
CCCCCCCEEEEEECCCCCCCEEEECCCHHHHHHHHHEEHHHHHHHHHHHHHHHHHHHHH
GSVEMHLEQIVRLMCEAARAGQVVVRLHSGDPALYGAIHEQMAALDEAGVPYEIVPGVTA
HHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCHHHHHHHHHHHHHHHCCCCEEECCCHHH
AFALAARLGVELTVPELVQTIILTRPAGRTPLPEHEQLSRMAAHGASLAIYLGITRMQQV
HHHHHHHHCCEEEHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHCCCCEEEEHHHHHHHHH
VRDLLTGGVYTPDTPVVVAYRITWPDEVIIHGTLGDIAEKVKAAGFTRQALILVSPALDP
HHHHHHCCCCCCCCCEEEEEEEECCCCEEEECCHHHHHHHHHHCCCCCEEEEEEECCCCC
ANKRTDRPTSRLYDPTYSHRLRRRREPADETAEA
CCCCCCCCHHHCCCCHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: S-adenosyl-L-methionine; Precorrin 4

Specific reaction: S-adenosyl-L-methionine + precorrin-4 = S-adenosyl-L-homocysteine + precorrin-5

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]