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The map label for this gene is cbiH [H]

Identifier: 222526016

GI number: 222526016

Start: 3416158

End: 3418509

Strand: Reverse

Name: cbiH [H]

Synonym: Chy400_2772

Alternate gene names: 222526016

Gene position: 3418509-3416158 (Counterclockwise)

Preceding gene: 222526017

Following gene: 222526015

Centisome position: 64.88

GC content: 63.1

Gene sequence:

>2352_bases
ATGACAACAACAGCCATCTTTGCCCTCACCCGTAACGGCGTTGAACTGGCCACTCGCCTGGCCGCAACGTTACCGGCAAC
TATCTGGCTCCCAGAGCGGTTTGCTGCGTTTGCGCCTGGTGGACGCTGCTACACCAACCTCAGCGCAGCCGTGCAAACAG
CCTGGCAACAGAGCAAAGCAATCATTCTGATCGCGGCTACCGGCATTGCTGTACGCCTGATTGCCCCGCTCCTCCAGGCG
AAGACCAGCGATCCGGCTGTGATCTGCCTTGATGAACAGGGACATGTAGTCGTGCCGTTGATCGGTGGTCATCGCGCCGG
TGCCAATGCCCTGGCCCGGCAGATCGCTGCACTCACCGGAGGTCAGGCTGCGATCACCACCGCCAGCGATGGGCAGGGGT
TACCGGCGCTCGATCTGATCGGTCAGGCGCAGGGCTGGCGCATCGCCACCGACTCGGCCACCACCCACGTGATGGCGTGT
CTGGTCAATGGTGATCCGATTGGGGTCTGGGTTGACCCCGACCTACCCGCTGCACGGGCACTGCTCGGCGCCGAACTGGC
ACCGGTAGCGACAGTCGAATGGGTCGCCGATTCCGAAGAGCTAACCAACCCACGCTTCGCAGCAGCCATTGTTGTCTCAC
ACCGTCGCCTCGATCCGCTGTGGCACAAACTACGCGATAAAGGGTTGCGGTACATGCCTCCGGTACTGGTGATCGGGATC
GGGTGTCGGCGCGATGTACCGGTACACGAACTCGCTACCGCCGTCTCCGCGACCCTGGCGACCGCCGATCTTGCCCCGGA
GTGCGTGGCAACTATTGCGACCGCCGATCTCAAGGCTGACGAAGCCGGCATCAGCGATCTGGCGCGGCAACTCGGTGTAC
CGCTCACTATCGTCACAACTGCTCAGCTCCAGACCCTTGACCCGACAGCCTTTAGCCCCAGTGCGGCCAGTCGCTTCGAT
ATTCCCGGTGTGGCCGAACCCTGCGCCACCCTGGTCGCGCAGGGGCCACTGCTGGTACCGAAACAGCGCTTTGCCCGTTG
CACCGTCGCTGTTGCTTTGCAGCAGGCAACGTTCGGTAGCGACACCACACCGACCGGCCAGCTCACCCTGGTCAGCATCG
GCCCTGGCGATCTCGCCCACCTGACCGAAGCGGCGCGACTGGCCCTGATCAAAGCCGAGGTCATCACCGGTTATGCCCGC
TACATCGATCTGATCCGGCCCCTGCTGCGTCCAGACCAGGAAGTTATCGCAACACCGGCGATGGGTGACGAGATGGGGCG
AGCGCGCCACGCCATTGAGCTGGCCCGCAGCGGGCGGCGGGTGGCGCTGATCAGCAGCGGAGACATCGGCATCTACGCTA
TGGCCGCACCGGTCTTCGAGAACCTTCAGGCCGGCGGCTGGGATGGGCGACACCCACAAGTCGAGGTCATTCCGGGGGTG
AGCGCATTTCAGGCACTGGCCGCCCGCATCGGCGCTCCGATCAACCACGATCTCTGCCTGATCAGCCTGAGCGACCTGCT
CACGCCATGGCCTCTGATCGAACGCCGGCTACGAGCTGCGGCACATGCCGACTTTGTCGTTGCCCTATACAACCCCCGCT
CGCAGGGTCGAAACTGGCAACTGGCCACAGCACTCTCTATCCTGCGCGACCACCGACCGGCAACGACACCGGTCGTCTTT
GGCCGGCAGGTGAGTCGCGAAGACGAACAGATTACGATAACCACCCTGGCCGACGCCGATCCGCAACAGGCCGACATGCT
GACCCTGGTGCTAATTGGGAACAGTCAGAGCTTTCATCTGGCCGGCCACGTCGTCACCCCGCGTGGCTACACGACCCAAC
CAGCCCGACCGTCAGACTTTCTTATGTCAAACAAAACAACAGATTACCCGATTGTGATCACCAAACCGGCCCACATGCCG
GCAGTGGTGATTGGTGGTGGTGCGGTTGGCGAACGCAAAGTCCGTGGCTTGCTGGCCGCCGGCATACCGGTACGGCTCAT
CAGCCCAACCGCAACCGACCAACTGATGGCGTGGGCGCAGGAAGGGCGCCTGATCTGGGAACGGCGCACATACCAGTCCG
GCGACCTCACCGGTGCCCGGTTGGTCTTCGCCGCCACCAATGATCGTGCAGTCAATGCCCGCATCGCTGCGGCGGCGATT
GCCGCCGGCGCACTCTGCAATGTCGCCGATGCCCCAGACGAAGGCGATTTTCACGTTCCGGCAATCTATCGCAGTGGCGG
GATCACGATTACGGTGAGCAGCGCCGGCACAGCCCCAGGTCGGGCCGTTGCCTTGCGCGATGCAATCGCCGATTGGCTAG
ACAGCATTGGAGTTCACAACCATGAGCGGTAA

Upstream 100 bases:

>100_bases
ACCAGCCGGCTCTACGATCCAACCTACAGTCATCGGTTGCGCCGTCGCCGTGAACCGGCTGATGAGACGGCAGAGGCATA
ACAGCCGGGAAGAGGTTCGT

Downstream 100 bases:

>100_bases
AGCATACCTTGTCGGCGCCGGCCCCGGTCGCGCCGATCTGATCACAGTGCGTGGGTTGACGGTGTTGCGCCAGGCTGATG
TCGTGCTCTACGACCGGCTG

Product: precorrin-3B C17-methyltransferase

Products: S-adenosyl-L-homocysteine; Precorrin 4

Alternate protein names: Cobalt-precorrin-3 methylase; Cobalt-precorrin-3 methyltransferase [H]

Number of amino acids: Translated: 783; Mature: 782

Protein sequence:

>783_residues
MTTTAIFALTRNGVELATRLAATLPATIWLPERFAAFAPGGRCYTNLSAAVQTAWQQSKAIILIAATGIAVRLIAPLLQA
KTSDPAVICLDEQGHVVVPLIGGHRAGANALARQIAALTGGQAAITTASDGQGLPALDLIGQAQGWRIATDSATTHVMAC
LVNGDPIGVWVDPDLPAARALLGAELAPVATVEWVADSEELTNPRFAAAIVVSHRRLDPLWHKLRDKGLRYMPPVLVIGI
GCRRDVPVHELATAVSATLATADLAPECVATIATADLKADEAGISDLARQLGVPLTIVTTAQLQTLDPTAFSPSAASRFD
IPGVAEPCATLVAQGPLLVPKQRFARCTVAVALQQATFGSDTTPTGQLTLVSIGPGDLAHLTEAARLALIKAEVITGYAR
YIDLIRPLLRPDQEVIATPAMGDEMGRARHAIELARSGRRVALISSGDIGIYAMAAPVFENLQAGGWDGRHPQVEVIPGV
SAFQALAARIGAPINHDLCLISLSDLLTPWPLIERRLRAAAHADFVVALYNPRSQGRNWQLATALSILRDHRPATTPVVF
GRQVSREDEQITITTLADADPQQADMLTLVLIGNSQSFHLAGHVVTPRGYTTQPARPSDFLMSNKTTDYPIVITKPAHMP
AVVIGGGAVGERKVRGLLAAGIPVRLISPTATDQLMAWAQEGRLIWERRTYQSGDLTGARLVFAATNDRAVNARIAAAAI
AAGALCNVADAPDEGDFHVPAIYRSGGITITVSSAGTAPGRAVALRDAIADWLDSIGVHNHER

Sequences:

>Translated_783_residues
MTTTAIFALTRNGVELATRLAATLPATIWLPERFAAFAPGGRCYTNLSAAVQTAWQQSKAIILIAATGIAVRLIAPLLQA
KTSDPAVICLDEQGHVVVPLIGGHRAGANALARQIAALTGGQAAITTASDGQGLPALDLIGQAQGWRIATDSATTHVMAC
LVNGDPIGVWVDPDLPAARALLGAELAPVATVEWVADSEELTNPRFAAAIVVSHRRLDPLWHKLRDKGLRYMPPVLVIGI
GCRRDVPVHELATAVSATLATADLAPECVATIATADLKADEAGISDLARQLGVPLTIVTTAQLQTLDPTAFSPSAASRFD
IPGVAEPCATLVAQGPLLVPKQRFARCTVAVALQQATFGSDTTPTGQLTLVSIGPGDLAHLTEAARLALIKAEVITGYAR
YIDLIRPLLRPDQEVIATPAMGDEMGRARHAIELARSGRRVALISSGDIGIYAMAAPVFENLQAGGWDGRHPQVEVIPGV
SAFQALAARIGAPINHDLCLISLSDLLTPWPLIERRLRAAAHADFVVALYNPRSQGRNWQLATALSILRDHRPATTPVVF
GRQVSREDEQITITTLADADPQQADMLTLVLIGNSQSFHLAGHVVTPRGYTTQPARPSDFLMSNKTTDYPIVITKPAHMP
AVVIGGGAVGERKVRGLLAAGIPVRLISPTATDQLMAWAQEGRLIWERRTYQSGDLTGARLVFAATNDRAVNARIAAAAI
AAGALCNVADAPDEGDFHVPAIYRSGGITITVSSAGTAPGRAVALRDAIADWLDSIGVHNHER
>Mature_782_residues
TTTAIFALTRNGVELATRLAATLPATIWLPERFAAFAPGGRCYTNLSAAVQTAWQQSKAIILIAATGIAVRLIAPLLQAK
TSDPAVICLDEQGHVVVPLIGGHRAGANALARQIAALTGGQAAITTASDGQGLPALDLIGQAQGWRIATDSATTHVMACL
VNGDPIGVWVDPDLPAARALLGAELAPVATVEWVADSEELTNPRFAAAIVVSHRRLDPLWHKLRDKGLRYMPPVLVIGIG
CRRDVPVHELATAVSATLATADLAPECVATIATADLKADEAGISDLARQLGVPLTIVTTAQLQTLDPTAFSPSAASRFDI
PGVAEPCATLVAQGPLLVPKQRFARCTVAVALQQATFGSDTTPTGQLTLVSIGPGDLAHLTEAARLALIKAEVITGYARY
IDLIRPLLRPDQEVIATPAMGDEMGRARHAIELARSGRRVALISSGDIGIYAMAAPVFENLQAGGWDGRHPQVEVIPGVS
AFQALAARIGAPINHDLCLISLSDLLTPWPLIERRLRAAAHADFVVALYNPRSQGRNWQLATALSILRDHRPATTPVVFG
RQVSREDEQITITTLADADPQQADMLTLVLIGNSQSFHLAGHVVTPRGYTTQPARPSDFLMSNKTTDYPIVITKPAHMPA
VVIGGGAVGERKVRGLLAAGIPVRLISPTATDQLMAWAQEGRLIWERRTYQSGDLTGARLVFAATNDRAVNARIAAAAIA
AGALCNVADAPDEGDFHVPAIYRSGGITITVSSAGTAPGRAVALRDAIADWLDSIGVHNHER

Specific function: Methyltransferase that catalyzes the methylation of C-17 in cobalt-precorrin-3B to form cobalt-precorrin-4 [H]

COG id: COG1010

COG function: function code H; Precorrin-3B methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the precorrin methyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI1789768, Length=133, Percent_Identity=36.8421052631579, Blast_Score=77, Evalue=3e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000878
- InterPro:   IPR014777
- InterPro:   IPR014776
- InterPro:   IPR006363 [H]

Pfam domain/function: PF00590 TP_methylase [H]

EC number: 2.1.1.131

Molecular weight: Translated: 82584; Mature: 82452

Theoretical pI: Translated: 6.90; Mature: 6.90

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTTAIFALTRNGVELATRLAATLPATIWLPERFAAFAPGGRCYTNLSAAVQTAWQQSKA
CCCEEEEEEECCCHHHHHHHHHHCCCEEEECHHHHHCCCCCCHHHHHHHHHHHHHHCCCE
IILIAATGIAVRLIAPLLQAKTSDPAVICLDEQGHVVVPLIGGHRAGANALARQIAALTG
EEEEEECCHHHHHHHHHHHCCCCCCEEEEECCCCCEEEEEECCCCCCHHHHHHHHHHHCC
GQAAITTASDGQGLPALDLIGQAQGWRIATDSATTHVMACLVNGDPIGVWVDPDLPAARA
CCEEEEECCCCCCCCHHHHHCCCCCCEEEECCCCEEEEEEEECCCEEEEEECCCCHHHHH
LLGAELAPVATVEWVADSEELTNPRFAAAIVVSHRRLDPLWHKLRDKGLRYMPPVLVIGI
HHCCCCCCCEEEEEECCCHHHCCCCEEEEEEECCCCCCHHHHHHHHCCCCCCCCEEEEEE
GCRRDVPVHELATAVSATLATADLAPECVATIATADLKADEAGISDLARQLGVPLTIVTT
CCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCHHHHHHHHCCCEEEEEE
AQLQTLDPTAFSPSAASRFDIPGVAEPCATLVAQGPLLVPKQRFARCTVAVALQQATFGS
EEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCEECCHHHHHHHHHHHHHHHHCCCC
DTTPTGQLTLVSIGPGDLAHLTEAARLALIKAEVITGYARYIDLIRPLLRPDQEVIATPA
CCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHEECCC
MGDEMGRARHAIELARSGRRVALISSGDIGIYAMAAPVFENLQAGGWDGRHPQVEVIPGV
CCHHHHHHHHHHHHHHCCCEEEEEECCCCEEEEEHHHHHHHHCCCCCCCCCCEEEEECCH
SAFQALAARIGAPINHDLCLISLSDLLTPWPLIERRLRAAAHADFVVALYNPRSQGRNWQ
HHHHHHHHHHCCCCCCCEEEEEHHHHCCCCHHHHHHHHHHHCCCEEEEEECCCCCCCCEE
LATALSILRDHRPATTPVVFGRQVSREDEQITITTLADADPQQADMLTLVLIGNSQSFHL
HHHHHHHHHCCCCCCCCEEECCCCCCCCCEEEEEEECCCCCCCCCEEEEEEEECCCCEEE
AGHVVTPRGYTTQPARPSDFLMSNKTTDYPIVITKPAHMPAVVIGGGAVGERKVRGLLAA
EEEEECCCCCCCCCCCCCHHHCCCCCCCCCEEEECCCCCCEEEEECCCCCHHHHHHHHHC
GIPVRLISPTATDQLMAWAQEGRLIWERRTYQSGDLTGARLVFAATNDRAVNARIAAAAI
CCCEEEECCCCHHHHHHHHHCCCEEEEECCCCCCCCCCEEEEEEECCCCEEHHHHHHHHH
AAGALCNVADAPDEGDFHVPAIYRSGGITITVSSAGTAPGRAVALRDAIADWLDSIGVHN
HHHHHCCCCCCCCCCCEECCEEEECCCEEEEEECCCCCCCCCHHHHHHHHHHHHHCCCCC
HER
CCC
>Mature Secondary Structure 
TTTAIFALTRNGVELATRLAATLPATIWLPERFAAFAPGGRCYTNLSAAVQTAWQQSKA
CCEEEEEEECCCHHHHHHHHHHCCCEEEECHHHHHCCCCCCHHHHHHHHHHHHHHCCCE
IILIAATGIAVRLIAPLLQAKTSDPAVICLDEQGHVVVPLIGGHRAGANALARQIAALTG
EEEEEECCHHHHHHHHHHHCCCCCCEEEEECCCCCEEEEEECCCCCCHHHHHHHHHHHCC
GQAAITTASDGQGLPALDLIGQAQGWRIATDSATTHVMACLVNGDPIGVWVDPDLPAARA
CCEEEEECCCCCCCCHHHHHCCCCCCEEEECCCCEEEEEEEECCCEEEEEECCCCHHHHH
LLGAELAPVATVEWVADSEELTNPRFAAAIVVSHRRLDPLWHKLRDKGLRYMPPVLVIGI
HHCCCCCCCEEEEEECCCHHHCCCCEEEEEEECCCCCCHHHHHHHHCCCCCCCCEEEEEE
GCRRDVPVHELATAVSATLATADLAPECVATIATADLKADEAGISDLARQLGVPLTIVTT
CCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCHHHHHHHHCCCEEEEEE
AQLQTLDPTAFSPSAASRFDIPGVAEPCATLVAQGPLLVPKQRFARCTVAVALQQATFGS
EEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCEECCHHHHHHHHHHHHHHHHCCCC
DTTPTGQLTLVSIGPGDLAHLTEAARLALIKAEVITGYARYIDLIRPLLRPDQEVIATPA
CCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHEECCC
MGDEMGRARHAIELARSGRRVALISSGDIGIYAMAAPVFENLQAGGWDGRHPQVEVIPGV
CCHHHHHHHHHHHHHHCCCEEEEEECCCCEEEEEHHHHHHHHCCCCCCCCCCEEEEECCH
SAFQALAARIGAPINHDLCLISLSDLLTPWPLIERRLRAAAHADFVVALYNPRSQGRNWQ
HHHHHHHHHHCCCCCCCEEEEEHHHHCCCCHHHHHHHHHHHCCCEEEEEECCCCCCCCEE
LATALSILRDHRPATTPVVFGRQVSREDEQITITTLADADPQQADMLTLVLIGNSQSFHL
HHHHHHHHHCCCCCCCCEEECCCCCCCCCEEEEEEECCCCCCCCCEEEEEEEECCCCEEE
AGHVVTPRGYTTQPARPSDFLMSNKTTDYPIVITKPAHMPAVVIGGGAVGERKVRGLLAA
EEEEECCCCCCCCCCCCCHHHCCCCCCCCCEEEECCCCCCEEEEECCCCCHHHHHHHHHC
GIPVRLISPTATDQLMAWAQEGRLIWERRTYQSGDLTGARLVFAATNDRAVNARIAAAAI
CCCEEEECCCCHHHHHHHHHCCCEEEEECCCCCCCCCCEEEEEEECCCCEEHHHHHHHHH
AAGALCNVADAPDEGDFHVPAIYRSGGITITVSSAGTAPGRAVALRDAIADWLDSIGVHN
HHHHHCCCCCCCCCCCEECCEEEECCCEEEEEECCCCCCCCCHHHHHHHHHHHHHCCCCC
HER
CCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: S-adenosyl-L-methionine; Precorrin 3B

Specific reaction: S-adenosyl-L-methionine + precorrin-3B = S-adenosyl-L-homocysteine + precorrin-4

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8501034; 11677609 [H]