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The map label for this gene is ribE [H]

Identifier: 222525620

GI number: 222525620

Start: 2994224

End: 2994832

Strand: Direct

Name: ribE [H]

Synonym: Chy400_2371

Alternate gene names: 222525620

Gene position: 2994224-2994832 (Clockwise)

Preceding gene: 222525619

Following gene: 222525621

Centisome position: 56.83

GC content: 57.31

Gene sequence:

>609_bases
ATGTTTACCGGCATTGTTGAAGAGATGGGCACAGTTGCTCATATCGTAAGCACGCAAGAACAGGCCAACCAGATCACGAT
TACCTGTCGCACTGTGCTTGAGGGAACGCGTATCGGCGATAGCATTGCCGTCAATGGCACCTGTCTGACGGTGACGGCAC
TCACCGCAGATAGCTTCACCGTCGGCCTGAGTCCAGAGACCTTGCGTCGCACCAATCTGGGCCGGCTGCACGTCGGTGAC
CCGGTGAATCTCGAACGGGCACTGGCGTTTGGTGGTCGGATGGGTGGGCATTACGTGCAGGGGCACGTCGACGGCGTTGG
CGAGATTGTAGCGATTGTGCCTGAAGGTGACTCAAAGCGAGTGACAATCCGCCCGCCGGCTGCTTTGCTGCCGTATATCG
TCGAAAAAGGCTACATCGCGGTTGACGGCGTCAGCCTGACGGTGGCCGAGATGGGGCGTGAGACGTTTACCGTTGCCCTG
GTTGCGTATACCCAATCGGCAGTCATCATGGGCCGTCAGACGGTGGGAGCATTGGTGAATATAGAGGTTGATATTATCGC
CAAGTATGTTGAACGCCTGCTTGAGGCGTATCGCCAGGAGGCTCGCTAA

Upstream 100 bases:

>100_bases
CGCCGTTATCGGCTCCGGCGGCTTGAACAATACGACCAGGATGCGTTGTTGATTGCCACGGCAGTTGATGCACCGTGGTG
GGATGTTGAGGAGGTTGCTG

Downstream 100 bases:

>100_bases
TGTTCGCATCAGTGGAAGAAGCGCTGCGCGATTACGCGGCTGGCCGGATGATCATCATCGTTGATGATGCTGATCGCGAG
AATGAGGGCGATCTGGCCTG

Product: riboflavin synthase subunit alpha

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 202; Mature: 202

Protein sequence:

>202_residues
MFTGIVEEMGTVAHIVSTQEQANQITITCRTVLEGTRIGDSIAVNGTCLTVTALTADSFTVGLSPETLRRTNLGRLHVGD
PVNLERALAFGGRMGGHYVQGHVDGVGEIVAIVPEGDSKRVTIRPPAALLPYIVEKGYIAVDGVSLTVAEMGRETFTVAL
VAYTQSAVIMGRQTVGALVNIEVDIIAKYVERLLEAYRQEAR

Sequences:

>Translated_202_residues
MFTGIVEEMGTVAHIVSTQEQANQITITCRTVLEGTRIGDSIAVNGTCLTVTALTADSFTVGLSPETLRRTNLGRLHVGD
PVNLERALAFGGRMGGHYVQGHVDGVGEIVAIVPEGDSKRVTIRPPAALLPYIVEKGYIAVDGVSLTVAEMGRETFTVAL
VAYTQSAVIMGRQTVGALVNIEVDIIAKYVERLLEAYRQEAR
>Mature_202_residues
MFTGIVEEMGTVAHIVSTQEQANQITITCRTVLEGTRIGDSIAVNGTCLTVTALTADSFTVGLSPETLRRTNLGRLHVGD
PVNLERALAFGGRMGGHYVQGHVDGVGEIVAIVPEGDSKRVTIRPPAALLPYIVEKGYIAVDGVSLTVAEMGRETFTVAL
VAYTQSAVIMGRQTVGALVNIEVDIIAKYVERLLEAYRQEAR

Specific function: Riboflavin synthase is a bifunctional enzyme complex catalyzing the formation of riboflavin from 5-amino-6-(1'-D)- ribityl-amino-2,4(1H,3H)-pyrimidinedione and L-3,4-dihydrohy-2- butanone-4-phosphate via 6,7-dimethyl-8-lumazine. The alpha subunit catalyze

COG id: COG0307

COG function: function code H; Riboflavin synthase alpha chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 lumazine-binding repeats [H]

Homologues:

Organism=Escherichia coli, GI1787952, Length=204, Percent_Identity=39.7058823529412, Blast_Score=133, Evalue=8e-33,
Organism=Saccharomyces cerevisiae, GI6319733, Length=203, Percent_Identity=45.8128078817734, Blast_Score=157, Evalue=8e-40,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001783
- InterPro:   IPR017938 [H]

Pfam domain/function: PF00677 Lum_binding [H]

EC number: =2.5.1.9 [H]

Molecular weight: Translated: 21626; Mature: 21626

Theoretical pI: Translated: 5.28; Mature: 5.28

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFTGIVEEMGTVAHIVSTQEQANQITITCRTVLEGTRIGDSIAVNGTCLTVTALTADSFT
CCCCHHHHHHHHHHHHCCHHHCCEEEEEEEEEECCCCCCCEEEECCEEEEEEEEECCCEE
VGLSPETLRRTNLGRLHVGDPVNLERALAFGGRMGGHYVQGHVDGVGEIVAIVPEGDSKR
ECCCHHHHHHCCCCEEECCCCCCHHHHHHHCCCCCCEEEEEEECCCEEEEEEEECCCCCE
VTIRPPAALLPYIVEKGYIAVDGVSLTVAEMGRETFTVALVAYTQSAVIMGRQTVGALVN
EEECCCHHHHHHHHHCCEEEECCCEEEHHHCCCCEEEEEEEEECCCEEEEECHHHCEEEE
IEVDIIAKYVERLLEAYRQEAR
EHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MFTGIVEEMGTVAHIVSTQEQANQITITCRTVLEGTRIGDSIAVNGTCLTVTALTADSFT
CCCCHHHHHHHHHHHHCCHHHCCEEEEEEEEEECCCCCCCEEEECCEEEEEEEEECCCEE
VGLSPETLRRTNLGRLHVGDPVNLERALAFGGRMGGHYVQGHVDGVGEIVAIVPEGDSKR
ECCCHHHHHHCCCCEEECCCCCCHHHHHHHCCCCCCEEEEEEECCCEEEEEEEECCCCCE
VTIRPPAALLPYIVEKGYIAVDGVSLTVAEMGRETFTVALVAYTQSAVIMGRQTVGALVN
EEECCCHHHHHHHHHCCEEEECCCEEEHHHCCCCEEEEEEEEECCCEEEEECHHHCEEEE
IEVDIIAKYVERLLEAYRQEAR
EHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8522537 [H]