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The map label for this gene is kojP [H]

Identifier: 222525433

GI number: 222525433

Start: 2759372

End: 2761537

Strand: Reverse

Name: kojP [H]

Synonym: Chy400_2178

Alternate gene names: 222525433

Gene position: 2761537-2759372 (Counterclockwise)

Preceding gene: 222525434

Following gene: 222525432

Centisome position: 52.41

GC content: 58.22

Gene sequence:

>2166_bases
ATGTGGATACTTGCTGAACCATCATTCGAGCCAACCAAACAACATCACAAAGAGACCATCTTTACCATCGGGAACGGCTA
TCTTTCGACCCGCGGTGCCTTTGAAGAGGGCTATCCTAACGACCGTCGCGCCACTTTCATCCACGGGGTCTTTGATGATG
CGCCGATAGTCGTTACTGAACTCGCCAATGCACCCGACTGGCTGGCTACCCAGATTGTGCTTGACGGTGAGAAGTTCTCG
CTGGCTAGCGGTACCGTTCTGGCCTATCGGCGTGAACTTGACTTACGGACGGGGGTGCTGCGCCGTGAAGTGCGCTGGCA
ATCGCCCCAGGGTCGCGCGGCCACCATCATCTTTACCCGCTTTGCCTCACTGGCCGACCAGCACCTGCTGGCATTGCGCT
GCGAGATCATTCCTGAATTTGACGGGCAGGTTGAGCTGCGGAGCGCAATCAACGGTCAAACCGACAACGAAGGGTTGCTG
CACTGGCGACCAATTGCCCAGGGTCAGCTCGCCGATGGCACCGTCTTCTTACGCACGCGCACCCGCAAGAGCGGGATTGA
GCTGGCGCTGACCATGCGCGTAAGTGGTTCTGACACTGCAACCACCACCTTCTGGGATGTGGAGAATGTGCCAACCCTGC
AACAGGTCTATCAGGCGCAGGCCGGTGTCCCCATCGTAGTGACGAAGTTTGTCGGTGTTGCCACGTCGCGCGACGATGCC
AACCCCCTGGAGCTGGCGCATCGACACCTTCAGGCCGCAACTGGCTGGGATGAGGCGCTGGCGGCACAGCAGCAGGTCTG
GGCCAAAGAATGGGAGCGCTGCAATGTGGAAATCGAGGGCGATGAAGAGGCCGATCTGGCCGTGCGTTTCAGCATATTCC
AGCTCCTGATTGCAGCGCCACGTCACGACCCACGGGTCAATATCGGCGCCAAAACCCTCTCTGGGTTTGGCTATCGCGGT
CATGCCTTCTGGGATACCGAAATCTTCATGCTCCCGCTCTTCATCTACACTGCACCAGACATTGCCCGTAATTTGCTGGA
CTATCGTTATCTGACGCTACCGGCTGCCCGCGCTAAAGCCCGCGCTGCCGGCTACGAGGGTGCCTGGTACGCCTGGGAGA
GTGCCGACAGCGGCGAAGAGGTAACACCAACCTGGGTGCCGGATTTTCACGACAAAAAGAAACTGGCCCGCGTATGGACT
GGCGATCTGGCGATTCACATCTCTTCAGACGTTGCCTACGCGGTTCAACAATACTGGCAGGCCACCGGAGACGATGCGTG
GTACATCGAGCGCGGTGCCGAAATCGTTCTCGACACGGCCAAATTCTGCGCTGCTCGTGCTGAATGGCTGAACGACCGCG
GCTGCTATGGCTACACCGATGTGATCGGCCCGGACGAATATCACGATCACGTCAACAACAACGCCTATACGAACCTGCTG
GCGCAATGGAACCTGCGCGCTGCGCTGGAGACATTGGCCTGGCTTGACCAACACGCACCGCAGAAGGCTGCCGAGCTACG
CCAGCGCCTCGATCTTACGCCTGAACGTTTACAGCACTGGCAAACGGTAGCCGAGAAGATGTGTGTAAACGTTTCACCCG
ACGGCTTGATTGAACAATTCGACGGCTTCTTCAAGCTCAAGGATGTCAATCTGGCCGACTACGAACCACGCACCAAATCA
ATGCATGAAATCTTCGGCATTGAGGGTGCCAATGAATATCAGGCCATTAAGCAACCGGATGTGTTGATGTTGCAATACTT
GCTGCGCGAACACTACAGCGACAGCCAGATTCGGGTCAACTACAACTACTACACACCGCGCACCGATCACACCTACGGCT
CATCGCTTGGCCCACCGATCCAGGCCATTGTCGCCTGCCAGATGGGTGATGTTGCCGAAGCCTACGAACACTTCATTCGG
GCCGCACGCGCCGATCTACGCGATGTCCGTGGCAACGCCGGCGACGGTATCCACGCCGCGTCGGCGGGTGGTGTCTGGCA
AGCGGTGGTCTTTGGCTTTGGCGGCTTGCGCGTGCATCCAGACGGGAGCTGGGACATTCAACCGCGACTTCCCCAACACT
GGCAGCGTCTGACCTTCCGCTTTACGCTGCGGGGCAAAACCTATACCGTCACCTGCTACCCCGACGGACGTTACACCACT
GCGTAA

Upstream 100 bases:

>100_bases
CGTCGTTTTGTCACGTCGTACCAACGGGCAGCGCCGGGATGCGCTTGTTTCGCGGAGCTGCCCGCCTTCTTTTCGCAGTT
TATATCACACGAGAACGGGT

Downstream 100 bases:

>100_bases
CCAACAGCAGGAGTGCAATCCCCACCATTGCACTCCTGTTTGTTAATAGTTGTTTCACAAATGTGAACTAAAGGAATAGC
TTTATGACAATTCAAGGGTT

Product: Kojibiose phosphorylase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 721; Mature: 721

Protein sequence:

>721_residues
MWILAEPSFEPTKQHHKETIFTIGNGYLSTRGAFEEGYPNDRRATFIHGVFDDAPIVVTELANAPDWLATQIVLDGEKFS
LASGTVLAYRRELDLRTGVLRREVRWQSPQGRAATIIFTRFASLADQHLLALRCEIIPEFDGQVELRSAINGQTDNEGLL
HWRPIAQGQLADGTVFLRTRTRKSGIELALTMRVSGSDTATTTFWDVENVPTLQQVYQAQAGVPIVVTKFVGVATSRDDA
NPLELAHRHLQAATGWDEALAAQQQVWAKEWERCNVEIEGDEEADLAVRFSIFQLLIAAPRHDPRVNIGAKTLSGFGYRG
HAFWDTEIFMLPLFIYTAPDIARNLLDYRYLTLPAARAKARAAGYEGAWYAWESADSGEEVTPTWVPDFHDKKKLARVWT
GDLAIHISSDVAYAVQQYWQATGDDAWYIERGAEIVLDTAKFCAARAEWLNDRGCYGYTDVIGPDEYHDHVNNNAYTNLL
AQWNLRAALETLAWLDQHAPQKAAELRQRLDLTPERLQHWQTVAEKMCVNVSPDGLIEQFDGFFKLKDVNLADYEPRTKS
MHEIFGIEGANEYQAIKQPDVLMLQYLLREHYSDSQIRVNYNYYTPRTDHTYGSSLGPPIQAIVACQMGDVAEAYEHFIR
AARADLRDVRGNAGDGIHAASAGGVWQAVVFGFGGLRVHPDGSWDIQPRLPQHWQRLTFRFTLRGKTYTVTCYPDGRYTT
A

Sequences:

>Translated_721_residues
MWILAEPSFEPTKQHHKETIFTIGNGYLSTRGAFEEGYPNDRRATFIHGVFDDAPIVVTELANAPDWLATQIVLDGEKFS
LASGTVLAYRRELDLRTGVLRREVRWQSPQGRAATIIFTRFASLADQHLLALRCEIIPEFDGQVELRSAINGQTDNEGLL
HWRPIAQGQLADGTVFLRTRTRKSGIELALTMRVSGSDTATTTFWDVENVPTLQQVYQAQAGVPIVVTKFVGVATSRDDA
NPLELAHRHLQAATGWDEALAAQQQVWAKEWERCNVEIEGDEEADLAVRFSIFQLLIAAPRHDPRVNIGAKTLSGFGYRG
HAFWDTEIFMLPLFIYTAPDIARNLLDYRYLTLPAARAKARAAGYEGAWYAWESADSGEEVTPTWVPDFHDKKKLARVWT
GDLAIHISSDVAYAVQQYWQATGDDAWYIERGAEIVLDTAKFCAARAEWLNDRGCYGYTDVIGPDEYHDHVNNNAYTNLL
AQWNLRAALETLAWLDQHAPQKAAELRQRLDLTPERLQHWQTVAEKMCVNVSPDGLIEQFDGFFKLKDVNLADYEPRTKS
MHEIFGIEGANEYQAIKQPDVLMLQYLLREHYSDSQIRVNYNYYTPRTDHTYGSSLGPPIQAIVACQMGDVAEAYEHFIR
AARADLRDVRGNAGDGIHAASAGGVWQAVVFGFGGLRVHPDGSWDIQPRLPQHWQRLTFRFTLRGKTYTVTCYPDGRYTT
A
>Mature_721_residues
MWILAEPSFEPTKQHHKETIFTIGNGYLSTRGAFEEGYPNDRRATFIHGVFDDAPIVVTELANAPDWLATQIVLDGEKFS
LASGTVLAYRRELDLRTGVLRREVRWQSPQGRAATIIFTRFASLADQHLLALRCEIIPEFDGQVELRSAINGQTDNEGLL
HWRPIAQGQLADGTVFLRTRTRKSGIELALTMRVSGSDTATTTFWDVENVPTLQQVYQAQAGVPIVVTKFVGVATSRDDA
NPLELAHRHLQAATGWDEALAAQQQVWAKEWERCNVEIEGDEEADLAVRFSIFQLLIAAPRHDPRVNIGAKTLSGFGYRG
HAFWDTEIFMLPLFIYTAPDIARNLLDYRYLTLPAARAKARAAGYEGAWYAWESADSGEEVTPTWVPDFHDKKKLARVWT
GDLAIHISSDVAYAVQQYWQATGDDAWYIERGAEIVLDTAKFCAARAEWLNDRGCYGYTDVIGPDEYHDHVNNNAYTNLL
AQWNLRAALETLAWLDQHAPQKAAELRQRLDLTPERLQHWQTVAEKMCVNVSPDGLIEQFDGFFKLKDVNLADYEPRTKS
MHEIFGIEGANEYQAIKQPDVLMLQYLLREHYSDSQIRVNYNYYTPRTDHTYGSSLGPPIQAIVACQMGDVAEAYEHFIR
AARADLRDVRGNAGDGIHAASAGGVWQAVVFGFGGLRVHPDGSWDIQPRLPQHWQRLTFRFTLRGKTYTVTCYPDGRYTT
A

Specific function: Hydrolyzes kojibiose in the presence of an inorganic phosphoric acid to form D-glucose and beta-D-glucose-1-phosphoric acid. Can act with alpha-1,2-oligoglucans, such as selaginose, as substrate, but more slowly. Inactive when dissaccharides with linkages

COG id: COG1554

COG function: function code G; Trehalose and maltose hydrolases (possible phosphorylases)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 65 family [H]

Homologues:

Organism=Homo sapiens, GI187829418, Length=434, Percent_Identity=30.6451612903226, Blast_Score=142, Evalue=2e-33,
Organism=Escherichia coli, GI1787575, Length=725, Percent_Identity=33.9310344827586, Blast_Score=375, Evalue=1e-105,
Organism=Saccharomyces cerevisiae, GI6325283, Length=701, Percent_Identity=21.5406562054208, Blast_Score=106, Evalue=2e-23,
Organism=Drosophila melanogaster, GI24583760, Length=467, Percent_Identity=27.6231263383298, Blast_Score=147, Evalue=4e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008928
- InterPro:   IPR012341
- InterPro:   IPR011013
- InterPro:   IPR005194
- InterPro:   IPR005195
- InterPro:   IPR005196
- InterPro:   IPR017045 [H]

Pfam domain/function: PF03633 Glyco_hydro_65C; PF03632 Glyco_hydro_65m; PF03636 Glyco_hydro_65N [H]

EC number: =2.4.1.230 [H]

Molecular weight: Translated: 81389; Mature: 81389

Theoretical pI: Translated: 5.49; Mature: 5.49

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MWILAEPSFEPTKQHHKETIFTIGNGYLSTRGAFEEGYPNDRRATFIHGVFDDAPIVVTE
CEEECCCCCCCHHHHCCCEEEEECCCEECCCCCCCCCCCCCCCEEEEEEECCCCCEEEEE
LANAPDWLATQIVLDGEKFSLASGTVLAYRRELDLRTGVLRREVRWQSPQGRAATIIFTR
CCCCCCCEEEEEEECCCEEEECCCEEEEEEHHHHHHHHHHHHHHCCCCCCCCEEEEHHHH
FASLADQHLLALRCEIIPEFDGQVELRSAINGQTDNEGLLHWRPIAQGQLADGTVFLRTR
HHHHHHHHHHEEEEEECCCCCCCEEEHHHCCCCCCCCCCEEEECCCCCCCCCCEEEEEEC
TRKSGIELALTMRVSGSDTATTTFWDVENVPTLQQVYQAQAGVPIVVTKFVGVATSRDDA
CCCCCCEEEEEEEECCCCCCEEEEEECCCCCHHHHHHHHHCCCCEEEEEHHHCCCCCCCC
NPLELAHRHLQAATGWDEALAAQQQVWAKEWERCNVEIEGDEEADLAVRFSIFQLLIAAP
CHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCEEECCCCCCCHHHHHHHHHHHHHCC
RHDPRVNIGAKTLSGFGYRGHAFWDTEIFMLPLFIYTAPDIARNLLDYRYLTLPAARAKA
CCCCCEEECHHHHCCCCCCCCEEECCHHHHHHHHHHCCHHHHHHHHHHHEEECCHHHHHH
RAAGYEGAWYAWESADSGEEVTPTWVPDFHDKKKLARVWTGDLAIHISSDVAYAVQQYWQ
HHCCCCCCEEEECCCCCCCCCCCCCCCCCCHHHHHHHHCCCCEEEEECCHHHHHHHHHHH
ATGDDAWYIERGAEIVLDTAKFCAARAEWLNDRGCYGYTDVIGPDEYHDHVNNNAYTNLL
CCCCCEEEEECCCEEEEEHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHCCCCHHHHHH
AQWNLRAALETLAWLDQHAPQKAAELRQRLDLTPERLQHWQTVAEKMCVNVSPDGLIEQF
HHCCHHHHHHHHHHHHHCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHEECCCCCHHHHHC
DGFFKLKDVNLADYEPRTKSMHEIFGIEGANEYQAIKQPDVLMLQYLLREHYSDSQIRVN
CCEEEEEECCCCCCCCCHHHHHHHHCCCCCCHHHHHCCCHHHHHHHHHHHCCCCCEEEEE
YNYYTPRTDHTYGSSLGPPIQAIVACQMGDVAEAYEHFIRAARADLRDVRGNAGDGIHAA
EEEECCCCCCCCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCEEC
SAGGVWQAVVFGFGGLRVHPDGSWDIQPRLPQHWQRLTFRFTLRGKTYTVTCYPDGRYTT
CCCCHHHHHHHCCCCEEECCCCCCCCCCCCHHHHCEEEEEEEECCCEEEEEEECCCCCCC
A
C
>Mature Secondary Structure
MWILAEPSFEPTKQHHKETIFTIGNGYLSTRGAFEEGYPNDRRATFIHGVFDDAPIVVTE
CEEECCCCCCCHHHHCCCEEEEECCCEECCCCCCCCCCCCCCCEEEEEEECCCCCEEEEE
LANAPDWLATQIVLDGEKFSLASGTVLAYRRELDLRTGVLRREVRWQSPQGRAATIIFTR
CCCCCCCEEEEEEECCCEEEECCCEEEEEEHHHHHHHHHHHHHHCCCCCCCCEEEEHHHH
FASLADQHLLALRCEIIPEFDGQVELRSAINGQTDNEGLLHWRPIAQGQLADGTVFLRTR
HHHHHHHHHHEEEEEECCCCCCCEEEHHHCCCCCCCCCCEEEECCCCCCCCCCEEEEEEC
TRKSGIELALTMRVSGSDTATTTFWDVENVPTLQQVYQAQAGVPIVVTKFVGVATSRDDA
CCCCCCEEEEEEEECCCCCCEEEEEECCCCCHHHHHHHHHCCCCEEEEEHHHCCCCCCCC
NPLELAHRHLQAATGWDEALAAQQQVWAKEWERCNVEIEGDEEADLAVRFSIFQLLIAAP
CHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCEEECCCCCCCHHHHHHHHHHHHHCC
RHDPRVNIGAKTLSGFGYRGHAFWDTEIFMLPLFIYTAPDIARNLLDYRYLTLPAARAKA
CCCCCEEECHHHHCCCCCCCCEEECCHHHHHHHHHHCCHHHHHHHHHHHEEECCHHHHHH
RAAGYEGAWYAWESADSGEEVTPTWVPDFHDKKKLARVWTGDLAIHISSDVAYAVQQYWQ
HHCCCCCCEEEECCCCCCCCCCCCCCCCCCHHHHHHHHCCCCEEEEECCHHHHHHHHHHH
ATGDDAWYIERGAEIVLDTAKFCAARAEWLNDRGCYGYTDVIGPDEYHDHVNNNAYTNLL
CCCCCEEEEECCCEEEEEHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHCCCCHHHHHH
AQWNLRAALETLAWLDQHAPQKAAELRQRLDLTPERLQHWQTVAEKMCVNVSPDGLIEQF
HHCCHHHHHHHHHHHHHCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHEECCCCCHHHHHC
DGFFKLKDVNLADYEPRTKSMHEIFGIEGANEYQAIKQPDVLMLQYLLREHYSDSQIRVN
CCEEEEEECCCCCCCCCHHHHHHHHCCCCCCHHHHHCCCHHHHHHHHHHHCCCCCEEEEE
YNYYTPRTDHTYGSSLGPPIQAIVACQMGDVAEAYEHFIRAARADLRDVRGNAGDGIHAA
EEEECCCCCCCCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCEEC
SAGGVWQAVVFGFGGLRVHPDGSWDIQPRLPQHWQRLTFRFTLRGKTYTVTCYPDGRYTT
CCCCHHHHHHHCCCCEEECCCCCCCCCCCCHHHHCEEEEEEEECCCEEEEEEECCCCCCC
A
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA