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The map label for this gene is pyrB

Identifier: 222525301

GI number: 222525301

Start: 2590425

End: 2591351

Strand: Direct

Name: pyrB

Synonym: Chy400_2043

Alternate gene names: 222525301

Gene position: 2590425-2591351 (Clockwise)

Preceding gene: 222525300

Following gene: 222525302

Centisome position: 49.16

GC content: 58.25

Gene sequence:

>927_bases
ATGACTGAATTACGTCGTCATGCAATCGATCTCGATAACTTTAGTGCGACTGAGATCGAGGAGATTCTGGAAACGGCTGA
GAGTATGCGCGAGGTGCTGAGCCGTGAAATTAAGCAGGTACCGGCACTACGCGGTAAAACTGTGGTCAATATGTTTTTTG
AGGAGAGTACGCGCACGCGCATCTCGTTTGAGCTGGCAGCGCGGGCTTTGTCGGCAAATGTGGTCGCCTTTACCGCACGG
GGGAGCAGTGTCGAGAAGGGTGAGTCGCTGGTTGATACGGTGCGCACGCTGCAAGCGTTGGGTGCCGACATCATTGTTAT
GCGCCACAGTCAGTCGGGTGCGCCTTATCTGGTGGCGCGTCACTTTCGTGGCTCGCTTATCAATGCCGGCGATGGCCGCC
ATGCCCATCCTACGCAGGCACTGCTCGATCTCTATACGATGCAGAGTCGATTGGGACAAATCCGCGATCTGCACGTCGTT
ATTGTCGGCGATATTCTGCATAGCCGGGTGGTGCGCTCCAACTTGTGGGGCTTGACCCGTCTCGGTGCGCGGGTGACGCT
CTGTGGCCCTCCCACCCTGATCGGCCCGGCGGCGTTCTGGACGGCGACCTGGCCGCAGGTGCGGATTGCGTATGAACTCG
ACCCACTGCTGCCGGAAGCGGATGTGGTGATGGCGTTGCGTCTGCAAAAAGAACGGATGCAAAGCGGATTGCTGCCGGCG
TTGCGCGAGTACACTCGGATCTATGGCCTCACCAGCGAACGCCTGGCCCGCCTGCCGTCACATGCGATTGTGATGCATCC
CGGCCCGATGAATGAAGGGATCGAAATCTTTCCCGAAGTGGCTACCGCATCACCGGCAGTGATTGAAGAGCAGGTCACCA
ATGGCGTCGCAGTGCGGATGGCGTTACTCTATCGAATGGCCGGTTGA

Upstream 100 bases:

>100_bases
GTGGGGAAGAATGTACCAACCTCGCTCTCCGAGAGGGTGATGGTGCGTTTGCGCGAGACCGATGGGGTTGATGAGGTTGT
GATTCTGCGAGGTTCTGCCA

Downstream 100 bases:

>100_bases
GAGGAGATGGCAATGCGTTATCTGATTAAAAATGGCACGATTATCGATCCGGCCAATCGTGTAGCCACCATTGGCGATAT
TCTGGTTGCCAACGGTAAAG

Product: aspartate carbamoyltransferase catalytic subunit

Products: NA

Alternate protein names: Aspartate transcarbamylase; ATCase

Number of amino acids: Translated: 308; Mature: 307

Protein sequence:

>308_residues
MTELRRHAIDLDNFSATEIEEILETAESMREVLSREIKQVPALRGKTVVNMFFEESTRTRISFELAARALSANVVAFTAR
GSSVEKGESLVDTVRTLQALGADIIVMRHSQSGAPYLVARHFRGSLINAGDGRHAHPTQALLDLYTMQSRLGQIRDLHVV
IVGDILHSRVVRSNLWGLTRLGARVTLCGPPTLIGPAAFWTATWPQVRIAYELDPLLPEADVVMALRLQKERMQSGLLPA
LREYTRIYGLTSERLARLPSHAIVMHPGPMNEGIEIFPEVATASPAVIEEQVTNGVAVRMALLYRMAG

Sequences:

>Translated_308_residues
MTELRRHAIDLDNFSATEIEEILETAESMREVLSREIKQVPALRGKTVVNMFFEESTRTRISFELAARALSANVVAFTAR
GSSVEKGESLVDTVRTLQALGADIIVMRHSQSGAPYLVARHFRGSLINAGDGRHAHPTQALLDLYTMQSRLGQIRDLHVV
IVGDILHSRVVRSNLWGLTRLGARVTLCGPPTLIGPAAFWTATWPQVRIAYELDPLLPEADVVMALRLQKERMQSGLLPA
LREYTRIYGLTSERLARLPSHAIVMHPGPMNEGIEIFPEVATASPAVIEEQVTNGVAVRMALLYRMAG
>Mature_307_residues
TELRRHAIDLDNFSATEIEEILETAESMREVLSREIKQVPALRGKTVVNMFFEESTRTRISFELAARALSANVVAFTARG
SSVEKGESLVDTVRTLQALGADIIVMRHSQSGAPYLVARHFRGSLINAGDGRHAHPTQALLDLYTMQSRLGQIRDLHVVI
VGDILHSRVVRSNLWGLTRLGARVTLCGPPTLIGPAAFWTATWPQVRIAYELDPLLPEADVVMALRLQKERMQSGLLPAL
REYTRIYGLTSERLARLPSHAIVMHPGPMNEGIEIFPEVATASPAVIEEQVTNGVAVRMALLYRMAG

Specific function: Pyrimidine biosynthesis; second step. [C]

COG id: COG0540

COG function: function code F; Aspartate carbamoyltransferase, catalytic chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATCase/OTCase family

Homologues:

Organism=Homo sapiens, GI18105007, Length=309, Percent_Identity=35.5987055016181, Blast_Score=176, Evalue=4e-44,
Organism=Homo sapiens, GI38788445, Length=176, Percent_Identity=28.4090909090909, Blast_Score=67, Evalue=2e-11,
Organism=Escherichia coli, GI2367364, Length=311, Percent_Identity=34.0836012861736, Blast_Score=145, Evalue=3e-36,
Organism=Escherichia coli, GI1786469, Length=219, Percent_Identity=29.6803652968037, Blast_Score=71, Evalue=1e-13,
Organism=Escherichia coli, GI1790703, Length=197, Percent_Identity=29.4416243654822, Blast_Score=66, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI193204318, Length=335, Percent_Identity=33.134328358209, Blast_Score=124, Evalue=9e-29,
Organism=Saccharomyces cerevisiae, GI6322331, Length=314, Percent_Identity=33.4394904458599, Blast_Score=144, Evalue=2e-35,
Organism=Drosophila melanogaster, GI24642586, Length=312, Percent_Identity=33.0128205128205, Blast_Score=146, Evalue=1e-35,

Paralogues:

None

Copy number: 2080 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 14328 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): PYRB_CHLAA (A9WDU7)

Other databases:

- EMBL:   CP000909
- RefSeq:   YP_001635495.1
- ProteinModelPortal:   A9WDU7
- SMR:   A9WDU7
- GeneID:   5826342
- GenomeReviews:   CP000909_GR
- KEGG:   cau:Caur_1891
- HOGENOM:   HBG579429
- OMA:   MNRGLEI
- ProtClustDB:   PRK00856
- HAMAP:   MF_00001
- InterPro:   IPR006132
- InterPro:   IPR006130
- InterPro:   IPR006131
- InterPro:   IPR002082
- PRINTS:   PR00100
- PRINTS:   PR00101
- TIGRFAMs:   TIGR00670

Pfam domain/function: PF00185 OTCace; PF02729 OTCace_N; SSF53671 Asp/Orn_carbamoyltranf

EC number: =2.1.3.2

Molecular weight: Translated: 34065; Mature: 33934

Theoretical pI: Translated: 7.86; Mature: 7.86

Prosite motif: PS00097 CARBAMOYLTRANSFERASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTELRRHAIDLDNFSATEIEEILETAESMREVLSREIKQVPALRGKTVVNMFFEESTRTR
CCCHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCE
ISFELAARALSANVVAFTARGSSVEKGESLVDTVRTLQALGADIIVMRHSQSGAPYLVAR
EHHHHHHHHHHCCEEEEEECCCCCHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCEEEEH
HFRGSLINAGDGRHAHPTQALLDLYTMQSRLGQIRDLHVVIVGDILHSRVVRSNLWGLTR
HHCCCEEECCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEEEHHHHHHHHHHHHHHHHHH
LGARVTLCGPPTLIGPAAFWTATWPQVRIAYELDPLLPEADVVMALRLQKERMQSGLLPA
HCCEEEEECCCCCCCCHHHHCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHCCCHHH
LREYTRIYGLTSERLARLPSHAIVMHPGPMNEGIEIFPEVATASPAVIEEQVTNGVAVRM
HHHHHHHHCCCHHHHHHCCCCEEEECCCCCCCCHHHCCHHHCCCHHHHHHHHHCHHHHHH
ALLYRMAG
HHHHHHCC
>Mature Secondary Structure 
TELRRHAIDLDNFSATEIEEILETAESMREVLSREIKQVPALRGKTVVNMFFEESTRTR
CCHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCE
ISFELAARALSANVVAFTARGSSVEKGESLVDTVRTLQALGADIIVMRHSQSGAPYLVAR
EHHHHHHHHHHCCEEEEEECCCCCHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCEEEEH
HFRGSLINAGDGRHAHPTQALLDLYTMQSRLGQIRDLHVVIVGDILHSRVVRSNLWGLTR
HHCCCEEECCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEEEHHHHHHHHHHHHHHHHHH
LGARVTLCGPPTLIGPAAFWTATWPQVRIAYELDPLLPEADVVMALRLQKERMQSGLLPA
HCCEEEEECCCCCCCCHHHHCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHCCCHHH
LREYTRIYGLTSERLARLPSHAIVMHPGPMNEGIEIFPEVATASPAVIEEQVTNGVAVRM
HHHHHHHHCCCHHHHHHCCCCEEEECCCCCCCCHHHCCHHHCCCHHHHHHHHHCHHHHHH
ALLYRMAG
HHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA